spacer
spacer
Go to PDB code: 
protein links
Cytokine PDB id
1ilt
Jmol
Contents
Protein chains
143 a.a.* *
* Residue conservation analysis
* C-alpha coords only
PDB id:
1ilt
Name: Cytokine
Title: X-ray structure of interleukin-1 receptor antagonist at 2.0 angstroms resolution
Structure: Interleukin-1 receptor antagonist. Chain: a, b. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606
Resolution:
2.00Å     R-factor:   0.236    
Authors: B.J.Brandhuber,G.P.A.Vigers
Key ref: G.P.Vigers et al. (1994). X-ray structure of interleukin-1 receptor antagonist at 2.0-A resolution. J Biol Chem, 269, 12874-12879. PubMed id: 8175703
Date:
09-Mar-94     Release date:   01-Apr-95    
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P18510  (IL1RA_HUMAN) -  Interleukin-1 receptor antagonist protein
Seq:
Struc: 		177 a.a.
143 a.a.
Key:    PfamA domain  Secondary structure

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   4 terms 
  Biological process     immune response   3 terms 
  Biochemical function     protein binding     3 terms  

 

 
    Key reference    
 
 
J Biol Chem 269:12874-12879 (1994)
PubMed id: 8175703  
 
 
X-ray structure of interleukin-1 receptor antagonist at 2.0-A resolution.
G.P.Vigers, P.Caffes, R.J.Evans, R.C.Thompson, S.P.Eisenberg, B.J.Brandhuber.
 
  ABSTRACT  
 
Interleukin-1 receptor antagonist (IL-1ra) is a natural competitive antagonist of IL-1. In order to further elucidate the mechanism by which IL-1ra binds without activating the IL-1 receptor, we have solved the crystal structure of IL-1ra at 2.0-A resolution. IL-1ra has the same overall beta-trefoil fold as IL-1 alpha and IL-1 beta and has a very similar hydrophobic core. However, there are a number of structural differences between the molecules, including significant differences at the open end of the beta-barrel, which has been identified in IL-1 beta as a receptor binding site.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19836339 A.Lingel, T.M.Weiss, M.Niebuhr, B.Pan, B.A.Appleton, C.Wiesmann, J.F.Bazan, and W.J.Fairbrother (2009).
Structure of IL-33 and its interaction with the ST2 and IL-1RAcP receptors--insight into heterotrimeric IL-1 signaling complexes.
  Structure, 17, 1398-1410.
PDB code: 2kll
19298391 R.A.Skinner, R.M.Gibson, N.J.Rothwell, E.Pinteaux, and J.I.Penny (2009).
Transport of interleukin-1 across cerebromicrovascular endothelial cells.
  Br J Pharmacol, 156, 1115-1123.  
19134476 S.Krishnan, and A.A.Raibekas (2009).
Multistep aggregation pathway of human interleukin-1 receptor antagonist: kinetic, structural, and morphological characterization.
  Biophys J, 96, 199-208.  
17924426 J.R.Alford, B.S.Kendrick, J.F.Carpenter, and T.W.Randolph (2008).
High concentration formulations of recombinant human interleukin-1 receptor antagonist: II. Aggregation kinetics.
  J Pharm Sci, 97, 3005-3021.  
17914732 Z.Q.Wen, X.Cao, and A.Vance (2008).
Conformation and side chains environments of recombinant human interleukin-1 receptor antagonist (rh-IL-1ra) probed by raman, raman optical activity, and UV-resonance Raman spectroscopy.
  J Pharm Sci, 97, 2228-2241.  
17445891 S.Datta, and R.R.Maclean (2007).
Neurobiological mechanisms for the regulation of mammalian sleep-wake behavior: reinterpretation of historical evidence and inclusion of contemporary cellular and molecular evidence.
  Neurosci Biobehav Rev, 31, 775-824.  
17847076 Z.Q.Wen (2007).
Raman spectroscopy of protein pharmaceuticals.
  J Pharm Sci, 96, 2861-2878.  
15514986 Y.Zhang, S.Roy, L.S.Jones, S.Krishnan, B.A.Kerwin, B.S.Chang, M.C.Manning, T.W.Randolph, and J.F.Carpenter (2004).
Mechanism for benzyl alcohol-induced aggregation of recombinant human interleukin-1 receptor antagonist in aqueous solution.
  J Pharm Sci, 93, 3076-3089.  
  12401481 S.Bird, J.Zou, T.Wang, B.Munday, C.Cunningham, and C.J.Secombes (2002).
Evolution of interleukin-1beta.
  Cytokine Growth Factor Rev, 13, 483-502.  
  9646173 C.A.Dinarello (1998).
Interleukin-1, interleukin-1 receptors and interleukin-1 receptor antagonist.
  Int Rev Immunol, 16, 457-499.  
  9865956 H.C.Wang, J.Dopazo, L.G.de la Fraga, Y.P.Zhu, and J.M.Carazo (1998).
Self-organizing tree-growing network for the classification of protein sequences.
  Protein Sci, 7, 2613-2622.  
9597123 W.P.Arend, M.Malyak, C.J.Guthridge, and C.Gabay (1998).
Interleukin-1 receptor antagonist: role in biology.
  Annu Rev Immunol, 16, 27-55.  
9342337 B.S.Kendrick, B.S.Chang, T.Arakawa, B.Peterson, T.W.Randolph, M.C.Manning, and J.F.Carpenter (1997).
Preferential exclusion of sucrose from recombinant interleukin-1 receptor antagonist: role in restricted conformational mobility and compaction of native state.
  Proc Natl Acad Sci U S A, 94, 11917-11922.  
9620641 C.A.Dinarello (1997).
Interleukin-1.
  Cytokine Growth Factor Rev, 8, 253-265.  
8968609 B.S.Chang, R.M.Beauvais, T.Arakawa, L.O.Narhi, A.Dong, D.I.Aparisio, and J.F.Carpenter (1996).
Formation of an active dimer during storage of interleukin-1 receptor antagonist in aqueous solution.
  Biophys J, 71, 3399-3406.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.