PDBsum entry: 1ihd

Hydrolase

PDB id: 1ihd

Contents

Protein chains

307 a.a. *

Waters ×54

* Residue conservation analysis

PDB id:

1ihd

Name:

Hydrolase

Title:

Crystal structure of trigonal form of d90e mutant of escheri asparaginase ii

Structure:

L-asparaginase ii. Chain: a, c. Synonym: l-asnase ii. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Tetramer (from PDB file)

Resolution:

2.65Å R-factor: 0.169 R-free: 0.209

Authors:

D.Borek,M.Jaskolski

Key ref:

D.Borek and m.jaskolski Structure analysis of d90e mutant of e.Coli asparaginase ii in three crystal forms. To be published,

Date:

19-Apr-01 Release date: 09-Sep-03

Protein chains

P00805  (ASPG2_ECOLI) -  L-asparaginase 2

Seq: 348 a.a.

Struc: 307 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.5.1.1 - Asparaginase.
• Reaction: L-asparagine + H₂O = L-aspartate + NH₃

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Cellular component: periplasmic space (2 terms)
• Biological process: cellular amino acid metabolic process (2 terms)
• Biochemical function: hydrolase activity (2 terms)