PDBsum entry: 1ihd

Hydrolase

PDB-id: 1ihd

Asymmetric unit

Contents

Description

Header details

Header records

References

PROCHECK

Protein chains

307 a.a. *

Waters ×54

* Residue conservation analysis

Tools

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Biological unit, tetramer
- as defined in PDB file (see also PQS)

PDB id:

1ihd

Name:

Hydrolase

Title:

Crystal structure of trigonal form of d90e mutant of escherichia coli asparaginase ii

Structure:

L-asparaginase ii. Chain: a, c. Synonym: l-asnase ii. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biological unit:

Tetramer (from PDB file)

UniProt:

Chains A, C: P00805 (ASPG2_ECOLI)

Seq:

Struc:

Seq:

348 a.a.

Struc:

307 a.a.*

Key:	PfamA domain
Secondary structure	CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme class:

E.C.3.5.1.1   [IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

Reaction:

L-asparagine + H₂O = L-aspartate + NH₃ (see diagram below)

Resolution:

2.65Å

R-factor:

0.169

R-free:

0.209

Authors:

D.Borek,M.Jaskolski

Key ref:

d.borek and m.jaskolski Structure Analysis of D90E Mutant of E.coli Asparaginase II in Three Crystal Forms. To be Published, .

Date:

19-Apr-01

Release date:

09-Sep-03

Related entries:

3eca
structure of native escherichia coli asparaginase ii
4eca
structure of t89v mutant of escherichia coli asparaginase i
1ho3
structure of y25f mutant of escherichia coli asparaginase i
1agx
structure of acinetobacter glutaminasificans glutaminase
asparaginase
1djo
... plus others (see Header records)

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