PDBsum entry: 1hxa

Lyase

PDB id: 1hxa

Contents

Protein chain

528 a.a. *

Ligands

FHP

Metals

_MG ×3

Waters ×209

* Residue conservation analysis

PDB id:

1hxa

Name:

Lyase

Title:

Crystal structure of teas w273s form 2

Structure:

5-epi-aristolochene synthase. Chain: a. Synonym: teas. Engineered: yes. Mutation: yes

Source:

Nicotiana tabacum. Common tobacco. Organism_taxid: 4097. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.32Å R-factor: 0.225 R-free: 0.246

Authors:

C.S.Starks,K.A.Rising,J.Chappell,J.P.Noel

Key ref:

C.S.Starks et al. Single active site mutations change the specificity of a sesquiterpene cyclase. To be published,

Date:

12-Jan-01 Release date: 24-Jun-03

Protein chain

Q40577  (5EAS_TOBAC) -  5-epi-aristolochene synthase

Seq: 548 a.a.

Struc: 528 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.4.2.3.61 - 5-epiaristolochene synthase.
• Reaction: (2E,6E)-farnesyl diphosphate = ⁺-5-epiaristolochene + diphosphate

(2E,6E)-farnesyl diphosphate = (+)-5-epiaristolochene (Bound ligand (Het Group name = FHP) matches with 66.67% similarity) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Cellular component: cytoplasm (1 term)
• Biological process: metabolic process (1 term)
• Biochemical function: lyase activity (4 terms)