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C-type lectin PDB id
1hup
Jmol
Contents
Protein chain
141 a.a. *
Ligands
SO4
Metals
_CA ×4
Waters ×25
* Residue conservation analysis
PDB id:
1hup
Name: C-type lectin
Title: Human mannose binding protein carbohydrate recognition domai trimerizes through a triple alpha-helical coiled-coil
Structure: Mannose-binding protein. Chain: a. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: mus musculus. Expression_system_taxid: 10090
Biol. unit: Trimer (from PQS)
Resolution:
2.50Å     R-factor:   not given    
Authors: S.Sheriff
Key ref: S.Sheriff et al. (1994). Human mannose-binding protein carbohydrate recognition domain trimerizes through a triple alpha-helical coiled-coil. Nat Struct Biol, 1, 789-794. PubMed id: 7634089 DOI: 10.1038/nsb1194-789
Date:
21-Sep-94     Release date:   15-Oct-95    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P11226  (MBL2_HUMAN) -  Mannose-binding protein C
Seq:
Struc: 		248 a.a.
141 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biochemical function     binding     1 term  

 

 
DOI no: 10.1038/nsb1194-789 Nat Struct Biol 1:789-794 (1994)
PubMed id: 7634089  
 
 
Human mannose-binding protein carbohydrate recognition domain trimerizes through a triple alpha-helical coiled-coil.
S.Sheriff, C.Y.Chang, R.A.Ezekowitz.
 
  ABSTRACT  
 
Human mannose-binding protein is a hexamer of trimers with each subunit consisting of an amino-terminal region rich in cysteine, 19 collagen repeats, a 'neck', and a carbohydrate recognition domain that requires calcium to bind ligand. A 148-residue peptide, consisting of the 'neck' and carbohydrate recognition domains forms trimers in solution and in crystals. The structure of this trimeric peptide has been determined in two different crystal forms. The 'neck' forms a triple alpha-helical coiled-coil. Each alpha-helix interacts with a neighbouring carbohydrate recognition domain. The spatial arrangement of the carbohydrate recognition domains suggest how MBP trimers form the basic recognition unit for branched oligosaccharides on microorganisms.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21091907 M.M.Dean, R.L.Flower, D.P.Eisen, R.M.Minchinton, D.N.Hart, and S.Vuckovic (2011).
Mannose-binding lectin deficiency influences innate and antigen-presenting functions of blood myeloid dendritic cells.
  Immunology, 132, 296-305.  
21383675 M.Wang, Y.Chen, Y.Zhang, L.Zhang, X.Lu, and Z.Chen (2011).
Mannan-binding lectin directly interacts with Toll-like receptor 4 and suppresses lipopolysaccharide-induced inflammatory cytokine secretion from THP-1 cells.
  Cell Mol Immunol, 8, 265-275.  
21194488 M.Wang, Y.Zhang, Y.Chen, L.Zhang, X.Lu, and Z.Chen (2011).
Mannan-binding lectin regulates dendritic cell maturation and cytokine production induced by lipopolysaccharide.
  BMC Immunol, 12, 1.  
20676430 B.Apostolovic, M.Danial, and H.A.Klok (2010).
Coiled coils: attractive protein folding motifs for the fabrication of self-assembled, responsive and bioactive materials.
  Chem Soc Rev, 39, 3541-3575.  
20338057 C.Miller, S.Wilgenbusch, M.Michaels, D.S.Chi, G.Youngberg, and G.Krishnaswamy (2010).
Molecular defects in the mannose binding lectin pathway in dermatological disease: Case report and literature review.
  Clin Mol Allergy, 8, 6.  
19218213 P.Guardado-Calvo, G.C.Fox, A.L.Llamas-Saiz, and M.J.van Raaij (2009).
Crystallographic structure of the {alpha}-helical triple coiled-coil domain of avian reovirus S1133 fibre.
  J Gen Virol, 90, 672-677.
PDB codes: 2jjl 2vrs
19631658 S.P.Boudko, T.Sasaki, J.Engel, T.F.Lerch, J.Nix, M.S.Chapman, and H.P.Bächinger (2009).
Crystal structure of human collagen XVIII trimerization domain: A novel collagen trimerization Fold.
  J Mol Biol, 392, 787-802.
PDB codes: 3hon 3hsh
19594626 W.K.Ip, K.Takahashi, R.A.Ezekowitz, and L.M.Stuart (2009).
Mannose-binding lectin and innate immunity.
  Immunol Rev, 230, 9.  
18070030 E.Acosta-Ramírez, R.Pérez-Flores, N.Majeau, R.Pastelin-Palacios, C.Gil-Cruz, M.Ramírez-Saldaña, N.Manjarrez-Orduño, L.Cervantes-Barragán, L.Santos-Argumedo, L.Flores-Romo, I.Becker, A.Isibasi, D.Leclerc, and C.López-Macías (2008).
Translating innate response into long-lasting antibody response by the intrinsic antigen-adjuvant properties of papaya mosaic virus.
  Immunology, 124, 186-197.  
18497748 R.Conners, D.J.Hill, E.Borodina, C.Agnew, S.J.Daniell, N.M.Burton, R.B.Sessions, A.R.Clarke, L.E.Catto, D.Lammie, T.Wess, R.L.Brady, and M.Virji (2008).
The Moraxella adhesin UspA1 binds to its human CEACAM1 receptor by a deformable trimeric coiled-coil.
  EMBO J, 27, 1779-1789.
PDB code: 2qih
18177377 R.Pagh, K.Duus, I.Laursen, P.R.Hansen, J.Mangor, N.Thielens, G.J.Arlaud, L.Kongerslev, P.Højrup, and G.Houen (2008).
The chaperone and potential mannan-binding lectin (MBL) co-receptor calreticulin interacts with MBL through the binding site for MBL-associated serine proteases.
  FEBS J, 275, 515-526.  
17497166 B.Roberts, B.Davidson, G.MacMaster, V.Lockhart, E.Ma, S.S.Wallace, and B.J.Swalla (2007).
A complement response may activate metamorphosis in the ascidian Boltenia villosa.
  Dev Genes Evol, 217, 449-458.  
17148457 M.Tanio, S.Kondo, S.Sugio, and T.Kohno (2007).
Trivalent recognition unit of innate immunity system: crystal structure of trimeric human M-ficolin fibrinogen-like domain.
  J Biol Chem, 282, 3889-3895.
PDB code: 2d39
17897951 V.Garlatti, L.Martin, E.Gout, J.B.Reiser, T.Fujita, G.J.Arlaud, N.M.Thielens, and C.Gaboriaud (2007).
Structural basis for innate immune sensing by M-ficolin and its control by a pH-dependent conformational switch.
  J Biol Chem, 282, 35814-35820.
PDB codes: 2jhh 2jhi 2jhk 2jhl 2jhm
17215869 V.Garlatti, N.Belloy, L.Martin, M.Lacroix, M.Matsushita, Y.Endo, T.Fujita, J.C.Fontecilla-Camps, G.J.Arlaud, N.M.Thielens, and C.Gaboriaud (2007).
Structural insights into the innate immune recognition specificities of L- and H-ficolins.
  EMBO J, 26, 623-633.
PDB codes: 2j0g 2j0h 2j0y 2j1g 2j2p 2j3f 2j3g 2j3o 2j3u 2j5z 2j60 2j61 2j64
16407218 J.N.Arnold, R.Wallis, A.C.Willis, D.J.Harvey, L.Royle, R.A.Dwek, P.M.Rudd, and R.B.Sim (2006).
Interaction of mannan binding lectin with alpha2 macroglobulin via exposed oligomannose glycans: a conserved feature of the thiol ester protein family?
  J Biol Chem, 281, 6955-6963.  
16368230 K.Takahashi, W.E.Ip, I.C.Michelow, and R.A.Ezekowitz (2006).
The mannose-binding lectin: a prototypic pattern recognition molecule.
  Curr Opin Immunol, 18, 16-23.  
16948640 R.M.Dommett, N.Klein, and M.W.Turner (2006).
Mannose-binding lectin in innate immunity: past, present and future.
  Tissue Antigens, 68, 193-209.  
15611777 E.Vivier, and B.Malissen (2005).
Innate and adaptive immunity: specificities and signaling hierarchies revisited.
  Nat Immunol, 6, 17-21.  
16150695 K.Okumura, A.Ohno, M.Nishida, K.Hayashi, K.Ikeda, and S.Inoue (2005).
Mapping the region of the alpha-type phospholipase A2 inhibitor responsible for its inhibitory activity.
  J Biol Chem, 280, 37651-37659.  
16299314 K.Takahashi, L.Shi, L.D.Gowda, and R.A.Ezekowitz (2005).
Relative roles of complement factor 3 and mannose-binding lectin in host defense against infection.
  Infect Immun, 73, 8188-8193.  
16189649 R.Sørensen, S.Thiel, and J.C.Jensenius (2005).
Mannan-binding-lectin-associated serine proteases, characteristics and disease associations.
  Springer Semin Immunopathol, 27, 299-319.  
  16511086 T.Ishigaki, I.Ohki, T.Oyama, S.Machida, K.Morikawa, and S.Tate (2005).
Purification, crystallization and preliminary X-ray analysis of the ligand-binding domain of human lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1).
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 61, 524-527.  
15148336 L.Shi, K.Takahashi, J.Dundee, S.Shahroor-Karni, S.Thiel, J.C.Jensenius, F.Gad, M.R.Hamblin, K.N.Sastry, and R.A.Ezekowitz (2004).
Mannose-binding lectin-deficient mice are susceptible to infection with Staphylococcus aureus.
  J Exp Med, 199, 1379-1390.  
15146501 T.A.Smith, P.M.Steinert, and D.A.Parry (2004).
Modeling effects of mutations in coiled-coil structures: case study using epidermolysis bullosa simplex mutations in segment 1a of K5/K14 intermediate filaments.
  Proteins, 55, 1043-1052.  
14660572 T.Ohashi, and H.P.Erickson (2004).
The disulfide bonding pattern in ficolin multimers.
  J Biol Chem, 279, 6534-6539.  
  12688114 A.Gruden-Movsesijan (2003).
[Mannose-binding protein--a factor in congenital immune reactions]
  Vojnosanit Pregl, 60, 67-75.  
12920133 A.McAlinden, T.A.Smith, L.J.Sandell, D.Ficheux, D.A.Parry, and D.J.Hulmes (2003).
Alpha-helical coiled-coil oligomerization domains are almost ubiquitous in the collagen superfamily.
  J Biol Chem, 278, 42200-42207.  
12792848 R.A.Ezekowitz (2003).
Role of the mannose-binding lectin in innate immunity.
  J Infect Dis, 187, S335-S339.  
12945048 S.Ebner, N.Sharon, and N.Ben-Tal (2003).
Evolutionary analysis reveals collective properties and specificity in the C-type lectin and lectin-like domain superfamily.
  Proteins, 53, 44-55.  
12524383 U.Holmskov, S.Thiel, and J.C.Jensenius (2003).
Collections and ficolins: humoral lectins of the innate immune defense.
  Annu Rev Immunol, 21, 547-578.  
11991814 H.Zhao, N.Wakamiya, Y.Suzuki, M.T.Hamonko, and G.L.Stahl (2002).
Identification of human mannose binding lectin (MBL) recognition sites for novel inhibitory antibodies.
  Hybrid Hybridomics, 21, 25-36.  
11850428 K.K.Ng, A.R.Kolatkar, S.Park-Snyder, H.Feinberg, D.A.Clark, K.Drickamer, and W.I.Weis (2002).
Orientation of bound ligands in mannose-binding proteins. Implications for multivalent ligand recognition.
  J Biol Chem, 277, 16088-16095.
PDB codes: 1kwt 1kwu 1kwv 1kww 1kwx 1kwy 1kwz 1kx0 1kx1 1kza 1kzb 1kzc 1kzd 1kze
11679589 L.J.Tai, S.M.McFall, K.Huang, B.Demeler, S.G.Fox, K.Brubaker, I.Radhakrishnan, and R.I.Morimoto (2002).
Structure-function analysis of the heat shock factor-binding protein reveals a protein composed solely of a highly conserved and dynamic coiled-coil trimerization domain.
  J Biol Chem, 277, 735-745.  
12438611 P.W.Hong, K.B.Flummerfelt, A.de Parseval, K.Gurney, J.H.Elder, and B.Lee (2002).
Human immunodeficiency virus envelope (gp120) binding to DC-SIGN and primary dendritic cells is carbohydrate dependent but does not involve 2G12 or cyanovirin binding sites: implications for structural analyses of gp120-DC-SIGN binding.
  J Virol, 76, 12855-12865.  
11181965 S.Hawgood, and F.R.Poulain (2001).
The pulmonary collectins and surfactant metabolism.
  Annu Rev Physiol, 63, 495-519.  
11120776 I.Ghiran, S.F.Barbashov, L.B.Klickstein, S.W.Tas, J.C.Jensenius, and A.Nicholson-Weller (2000).
Complement receptor 1/CD35 is a receptor for mannan-binding lectin.
  J Exp Med, 192, 1797-1808.  
  11045608 K.Håkansson, and K.B.Reid (2000).
Collectin structure: a review.
  Protein Sci, 9, 1607-1617.  
10858200 M.Devyatyarova-Johnson, I.H.Rees, B.D.Robertson, M.W.Turner, N.J.Klein, and D.L.Jack (2000).
The lipopolysaccharide structures of Salmonella enterica serovar Typhimurium and Neisseria gonorrhoeae determine the attachment of human mannose-binding lectin to intact organisms.
  Infect Immun, 68, 3894-3899.  
10828943 N.Palaniyar, F.X.McCormack, F.Possmayer, and G.Harauz (2000).
Three-dimensional structure of rat surfactant protein A trimers in association with phospholipid monolayers.
  Biochemistry, 39, 6310-6316.
PDB codes: 1du8 1gie
10639434 O.Neth, D.L.Jack, A.W.Dodds, H.Holzel, N.J.Klein, and M.W.Turner (2000).
Mannose-binding lectin binds to a range of clinically relevant microorganisms and promotes complement deposition.
  Infect Immun, 68, 688-693.  
10792532 P.J.Naughton, G.Grant, S.Bardocz, and A.Pusztai (2000).
Modulation of Salmonella infection by the lectins of Canavalia ensiformis (Con A) and Galanthus nivalis (GNA) in a rat model in vivo.
  J Appl Microbiol, 88, 720-727.  
10023772 J.C.Boyington, A.N.Riaz, A.Patamawenu, J.E.Coligan, A.G.Brooks, and P.D.Sun (1999).
Structure of CD94 reveals a novel C-type lectin fold: implications for the NK cell-associated CD94/NKG2 receptors.
  Immunity, 10, 75-82.
PDB code: 1b6e
10508765 K.Drickamer (1999).
C-type lectin-like domains.
  Curr Opin Struct Biol, 9, 585-590.  
  10368295 K.Håkansson, N.K.Lim, H.J.Hoppe, and K.B.Reid (1999).
Crystal structure of the trimeric alpha-helical coiled-coil and the three lectin domains of human lung surfactant protein D.
  Structure, 7, 255-264.
PDB code: 1b08
10224141 K.Ohtani, Y.Suzuki, S.Eda, T.Kawai, T.Kase, H.Yamazaki, T.Shimada, H.Keshi, Y.Sakai, A.Fukuoh, T.Sakamoto, and N.Wakamiya (1999).
Molecular cloning of a novel human collectin from liver (CL-L1).
  J Biol Chem, 274, 13681-13689.  
10196140 S.Iida, K.Yamamoto, and T.Irimura (1999).
Interaction of human macrophage C-type lectin with O-linked N-acetylgalactosamine residues on mucin glycopeptides.
  J Biol Chem, 274, 10697-10705.  
  10545330 V.A.Kostyuchenko, G.A.Navruzbekov, L.P.Kurochkina, S.V.Strelkov, V.V.Mesyanzhinov, and M.G.Rossmann (1999).
The structure of bacteriophage T4 gene product 9: the trigger for tail contraction.
  Structure, 7, 1213-1222.
PDB codes: 1qex 1s2e
9443889 R.A.Ezekowitz (1998).
Genetic heterogeneity of mannose-binding proteins: the Jekyll and Hyde of innate immunity?
  Am J Hum Genet, 62, 6-9.  
9640255 S.B.Laursen, T.S.Dalgaard, S.Thiel, B.L.Lim, T.V.Jensen, H.R.Juul-Madsen, A.Takahashi, T.Hamana, M.Kawakami, and J.C.Jensenius (1998).
Cloning and sequencing of a cDNA encoding chicken mannan-binding lectin (MBL) and comparison with mammalian analogues.
  Immunology, 93, 421-430.  
9720214 S.Eda, Y.Suzuki, T.Kawai, K.Ohtani, T.Kase, T.Sakamoto, and N.Wakamiya (1998).
Characterization of truncated human mannan-binding protein (MBP) expressed in Escherichia coli.
  Biosci Biotechnol Biochem, 62, 1326-1331.  
9700499 W.I.Weis, M.E.Taylor, and K.Drickamer (1998).
The C-type lectin superfamily in the immune system.
  Immunol Rev, 163, 19-34.  
9057819 H.J.Gabius (1997).
Animal lectins.
  Eur J Biochem, 243, 543-576.  
9108235 J.Johansson, and T.Curstedt (1997).
Molecular structures and interactions of pulmonary surfactant components.
  Eur J Biochem, 244, 675-693.  
9204768 J.Lu (1997).
Collectins: collectors of microorganisms for the innate immune system.
  Bioessays, 19, 509-518.  
9433125 S.Elgavish, and B.Shaanan (1997).
Lectin-carbohydrate interactions: different folds, common recognition principles.
  Trends Biochem Sci, 22, 462-467.  
  9232652 T.L.Holtet, J.H.Graversen, I.Clemmensen, H.C.Thøgersen, and M.Etzerodt (1997).
Tetranectin, a trimeric plasminogen-binding C-type lectin.
  Protein Sci, 6, 1511-1515.  
9345619 W.I.Weis (1997).
Cell-surface carbohydrate recognition by animal and viral lectins.
  Curr Opin Struct Biol, 7, 624-630.  
  9261070 Y.Tao, S.V.Strelkov, V.V.Mesyanzhinov, and M.G.Rossmann (1997).
Structure of bacteriophage T4 fibritin: a segmented coiled coil and the role of the C-terminal domain.
  Structure, 5, 789-798.
PDB code: 1aa0
8797823 D.Kohda, C.J.Morton, A.A.Parkar, H.Hatanaka, F.M.Inagaki, I.D.Campbell, and A.J.Day (1996).
Solution structure of the link module: a hyaluronan-binding domain involved in extracellular matrix stability and cell migration.
  Cell, 86, 767-775.
PDB code: 1tsg
9007273 H.Franzyk, M.Meldal, H.Paulsen, S.Thiel, J.C.Jensenius, and K.Bock (1996).
Glycopeptide mimics of mammalian Man9GlcNAc2. Ligand binding to mannan-binding proteins (MBPs).
  Bioorg Med Chem, 4, 1881-1899.  
  8654365 J.A.Bertrand, D.Pignol, J.P.Bernard, J.M.Verdier, J.C.Dagorn, and J.C.Fontecilla-Camps (1996).
Crystal structure of human lithostathine, the pancreatic inhibitor of stone formation.
  EMBO J, 15, 2678-2684.
PDB code: 1lit
  8745401 J.Bajorath, and A.Aruffo (1996).
Structure-based modeling of the ligand binding domain of the human cell surface receptor CD23 and comparison of two independently derived molecular models.
  Protein Sci, 5, 240-247.
PDB code: 1kje
8729443 J.Epstein, Q.Eichbaum, S.Sheriff, and R.A.Ezekowitz (1996).
The collectins in innate immunity.
  Curr Opin Immunol, 8, 29-35.  
8557671 K.K.Ng, K.Drickamer, and W.I.Weis (1996).
Structural analysis of monosaccharide recognition by rat liver mannose-binding protein.
  J Biol Chem, 271, 663-674.
PDB codes: 1rdi 1rdj 1rdk 1rdl 1rdm 1rdn 1rdo
8939653 P.Maurer, E.Hohenester, and J.Engel (1996).
Extracellular calcium-binding proteins.
  Curr Opin Cell Biol, 8, 609-617.  
8749360 D.I.Stuart, and E.Y.Jones (1995).
Recognition at the cell surface: recent structural insights.
  Curr Opin Struct Biol, 5, 735-743.  
8574697 J.M.Rini (1995).
X-ray crystal structures of animal lectins.
  Curr Opin Struct Biol, 5, 617-621.  
  7704523 H.J.Hoppe, and K.B.Reid (1994).
Trimeric C-type lectin domains in host defence.
  Structure, 2, 1129-1133.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.