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Cytokine (chemotactic) PDB id
1hrj
Jmol
Contents
Protein chains
68 a.a. *
* Residue conservation analysis
PDB id:
1hrj
Name: Cytokine (chemotactic)
Title: Human rantes, nmr, 13 structures
Structure: Human regulated upon activation normal t-cell expressed and secreted. Chain: a, b. Synonym: human rantes. Engineered: yes. Other_details: ph 3.2, 313 k
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562
NMR struc: 13 models
Authors: C.Chung,R.M.Cooke,A.E.I.Proudfoot,T.N.C.Wells
Key ref:
C.W.Chung et al. (1995). The three-dimensional solution structure of RANTES. Biochemistry, 34, 9307-9314. PubMed id: 7542919 DOI: 10.1021/bi00029a005
Date:
18-Aug-95     Release date:   14-Oct-96    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P13501  (CCL5_HUMAN) -  C-C motif chemokine 5
Seq:
Struc: 		91 a.a.
68 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   4 terms 
  Biological process     cellular response to fibroblast growth factor stimulus   97 terms 
  Biochemical function     chemoattractant activity     15 terms  

 

 
DOI no: 10.1021/bi00029a005 Biochemistry 34:9307-9314 (1995)
PubMed id: 7542919  
 
 
The three-dimensional solution structure of RANTES.
C.W.Chung, R.M.Cooke, A.E.Proudfoot, T.N.Wells.
 
  ABSTRACT  
 
The solution structure of the chemokine RANTES (regulated on activation, normal T-cell expressed and secreted) has been determined using NMR spectroscopy. Backbone and side-chain 1H and 15N assignments have been obtained using a combination of two-dimensional homonuclear and three-dimensional heteronuclear spectra. Regular elements of secondary structure have been identified on the basis of a qualitative interpretation of NOE data, J(NH-H alpha) coupling constants, and amide exchange rates. Three-dimensional structures were calculated from a total of 2146 experimental restraints using a combination of distance geometry and simulated annealing protocols. For the 13 best structures the average backbone (N, C alpha, C) atomic rmsd from the mean coordinates for residues 5-65 is 0.64 A (+/- 0.14 A) for the dimer and 0.50 A (+/- 0.08 A) for the individual monomers. Each monomer consists of a three-stranded antiparallel beta-sheet (residues 26-30, 38-43, 48-51) in a Greek key motif with a C-terminal helix (56-65) packed across the sheet, an arrangement similar to the monomeric structure of other members of this chemokine family (IL-8, PF4, MGSA/Gro alpha, and MIP-1 beta). Overall, the RANTES dimer resembles that previously reported for MIP-1 beta.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19573605 M.Secchi, Q.Xu, P.Lusso, and L.Vangelista (2009).
The superior folding of a RANTES analogue expressed in lactobacilli as compared to mammalian cells reveals a promising system to screen new RANTES mutants.
  Protein Expr Purif, 68, 34-41.  
19122657 R.R.Koenen, P.von Hundelshausen, I.V.Nesmelova, A.Zernecke, E.A.Liehn, A.Sarabi, B.K.Kramp, A.M.Piccinini, S.R.Paludan, M.A.Kowalska, A.J.Kungl, T.M.Hackeng, K.H.Mayo, and C.Weber (2009).
Disrupting functional interactions between platelet chemokines inhibits atherosclerosis in hyperlipidemic mice.
  Nat Med, 15, 97.  
18323622 C.Barinka, A.Prahl, and J.Lubkowski (2008).
Structure of human monocyte chemoattractant protein 4 (MCP-4/CCL13).
  Acta Crystallogr D Biol Crystallogr, 64, 273-278.
PDB code: 2ra4
18243436 L.Vangelista, M.Secchi, and P.Lusso (2008).
Rational design of novel HIV-1 entry inhibitors by RANTES engineering.
  Vaccine, 26, 3008-3015.  
17071104 C.T.Veldkamp, F.C.Peterson, P.L.Hayes, J.E.Mattmiller, J.C.Haugner, N.de la Cruz, and B.F.Volkman (2007).
On-column refolding of recombinant chemokines for NMR studies and biological assays.
  Protein Expr Purif, 52, 202-209.  
16810664 F.García-Martín, P.White, R.Steinauer, S.Côté, J.Tulla-Puche, and F.Albericio (2006).
The synergy of ChemMatrix resin and pseudoproline building blocks renders RANTES, a complex aggregated chemokine.
  Biopolymers, 84, 566-575.  
15979374 J.Y.Springael, E.Urizar, and M.Parmentier (2005).
Dimerization of chemokine receptors and its functional consequences.
  Cytokine Growth Factor Rev, 16, 611-623.  
11807180 E.J.Fernandez, and E.Lolis (2002).
Structure, function, and inhibition of chemokines.
  Annu Rev Pharmacol Toxicol, 42, 469-499.  
11889129 E.S.Kuloğlu, D.R.McCaslin, J.L.Markley, and B.F.Volkman (2002).
Structural rearrangement of human lymphotactin, a C chemokine, under physiological solution conditions.
  J Biol Chem, 277, 17863-17870.  
11470923 B.T.Seet, R.Singh, C.Paavola, E.K.Lau, T.M.Handel, and G.McFadden (2001).
Molecular determinants for CC-chemokine recognition by a poxvirus CC-chemokine inhibitor.
  Proc Natl Acad Sci U S A, 98, 9008-9013.  
11358512 W.Shao, E.Fernandez, A.Sachpatzidis, J.Wilken, D.A.Thompson, B.I.Schweitzer, and E.Lolis (2001).
CCR2 and CCR5 receptor-binding properties of herpesvirus-8 vMIP-II based on sequence analysis and its solution structure.
  Eur J Biochem, 268, 2948-2959.
PDB code: 1hhv
11041848 E.J.Fernandez, J.Wilken, D.A.Thompson, S.C.Peiper, and E.Lolis (2000).
Comparison of the structure of vMIP-II with eotaxin-1, RANTES, and MCP-3 suggests a unique mechanism for CCR3 activation.
  Biochemistry, 39, 12837-12844.
PDB code: 1cm9
11087354 J.Blaszczyk, E.V.Coillie, P.Proost, J.V.Damme, G.Opdenakker, G.D.Bujacz, J.M.Wang, and X.Ji (2000).
Complete crystal structure of monocyte chemotactic protein-2, a CC chemokine that interacts with multiple receptors.
  Biochemistry, 39, 14075-14081.
PDB code: 1esr
  11106181 J.T.Ashfield, T.Meyers, D.Lowne, P.G.Varley, J.R.Arnold, P.Tan, J.C.Yang, L.G.Czaplewski, T.Dudgeon, and J.Fisher (2000).
Chemical modification of a variant of human MIP-1alpha; implications for dimer structure.
  Protein Sci, 9, 2047-2053.  
11093134 S.Polo, V.Nardese, C.De Santis, C.Arcelloni, R.Paroni, F.Sironi, A.Verani, M.Rizzi, M.Bolognesi, and P.Lusso (2000).
Enhancement of the HIV-1 inhibitory activity of RANTES by modification of the N-terminal region: dissociation from CCR5 activation.
  Eur J Immunol, 30, 3190-3198.  
  10595530 A.C.Liwang, Z.X.Wang, Y.Sun, S.C.Peiper, and P.J.Liwang (1999).
The solution structure of the anti-HIV chemokine vMIP-II.
  Protein Sci, 8, 2270-2280.
PDB code: 1vmp
  10400729 A.Trkola, C.Gordon, J.Matthews, E.Maxwell, T.Ketas, L.Czaplewski, A.E.Proudfoot, and J.P.Moore (1999).
The CC-chemokine RANTES increases the attachment of human immunodeficiency virus type 1 to target cells via glycosaminoglycans and also activates a signal transduction pathway that enhances viral infectivity.
  J Virol, 73, 6370-6379.  
  10433951 E.Sato, K.L.Simpson, M.B.Grisham, S.Koyama, and R.A.Robbins (1999).
Effects of reactive oxygen and nitrogen metabolites on RANTES- and IL-5-induced eosinophil chemotactic activity in vitro.
  Am J Pathol, 155, 591-598.  
10320325 H.Sticht, S.E.Escher, K.Schweimer, W.G.Forssmann, P.Rösch, and K.Adermann (1999).
Solution structure of the human CC chemokine 2: A monomeric representative of the CC chemokine subtype.
  Biochemistry, 38, 5995-6002.
PDB code: 2hcc
9889151 J.Wilken, D.Hoover, D.A.Thompson, P.N.Barlow, H.McSparron, L.Picard, A.Wlodawer, J.Lubkowski, and S.B.Kent (1999).
Total chemical synthesis and high-resolution crystal structure of the potent anti-HIV protein AOP-RANTES.
  Chem Biol, 6, 43-51.
PDB code: 1b3a
10347159 L.G.Czaplewski, J.McKeating, C.J.Craven, L.D.Higgins, V.Appay, A.Brown, T.Dudgeon, L.A.Howard, T.Meyers, J.Owen, S.R.Palan, P.Tan, G.Wilson, N.R.Woods, C.M.Heyworth, B.I.Lord, D.Brotherton, R.Christison, S.Craig, S.Cribbes, R.M.Edwards, S.J.Evans, R.Gilbert, P.Morgan, E.Randle, N.Schofield, P.G.Varley, J.Fisher, J.P.Waltho, and M.G.Hunter (1999).
Identification of amino acid residues critical for aggregation of human CC chemokines macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, and RANTES. Characterization of active disaggregated chemokine variants.
  J Biol Chem, 274, 16077-16084.
PDB codes: 1b50 1b53
10419853 M.K.Schwarz, and T.N.Wells (1999).
Interfering with chemokine networks--the hope for new therapeutics.
  Curr Opin Chem Biol, 3, 407-417.  
10360735 Y.Nishiyama, T.Murakami, K.Kurita, and N.Yamamoto (1999).
Low-molecular-weight anti-HIV-1 peptides from the amino-terminal sequence of RANTES: possible lead compounds for coreceptor-directed anti-HIV-1 agents.
  Bioorg Med Chem Lett, 9, 1357-1360.  
9914924 B.Dixon, B.Shum, E.J.Adams, K.E.Magor, R.P.Hedrick, D.G.Muir, and P.Parham (1998).
CK-1, a putative chemokine of rainbow trout (Oncorhynchus mykiss).
  Immunol Rev, 166, 341-348.  
9837883 C.D.Paavola, S.Hemmerich, D.Grunberger, I.Polsky, A.Bloom, R.Freedman, M.Mulkins, S.Bhakta, D.McCarley, L.Wiesent, B.Wong, K.Jarnagin, and T.M.Handel (1998).
Monomeric monocyte chemoattractant protein-1 (MCP-1) binds and activates the MCP-1 receptor CCR2B.
  J Biol Chem, 273, 33157-33165.  
9618518 C.Dealwis, E.J.Fernandez, D.A.Thompson, R.J.Simon, M.A.Siani, and E.Lolis (1998).
Crystal structure of chemically synthesized [N33A] stromal cell-derived factor 1alpha, a potent ligand for the HIV-1 "fusin" coreceptor.
  Proc Natl Acad Sci U S A, 95, 6941-6946.
PDB code: 1a15
9662193 L.Ylisastigui, J.Vizzavona, E.Drakopoulou, P.Paindavoine, C.F.Calvo, M.Parmentier, J.C.Gluckman, C.Vita, and A.Benjouad (1998).
Synthetic full-length and truncated RANTES inhibit HIV-1 infection of primary macrophages.
  AIDS, 12, 977-984.  
9712872 M.P.Crump, K.Rajarathnam, K.S.Kim, I.Clark-Lewis, and B.D.Sykes (1998).
Solution structure of eotaxin, a chemokine that selectively recruits eosinophils in allergic inflammation.
  J Biol Chem, 273, 22471-22479.
PDB codes: 1eot 2eot
9662194 S.Menzo, R.Sampaolesi, E.Vicenzi, E.Santagostino, G.Liuzzi, A.Chirianni, M.Piazza, O.J.Cohen, P.Bagnarelli, and M.Clementi (1998).
Rare mutations in a domain crucial for V3-loop structure prevail in replicating HIV from long-term non-progressors.
  AIDS, 12, 985-997.  
9622482 W.Shao, L.F.Jerva, J.West, E.Lolis, and B.I.Schweitzer (1998).
Solution structure of murine macrophage inflammatory protein-2.
  Biochemistry, 37, 8303-8313.
PDB code: 1mi2
9164453 A.Wlodawer (1997).
Deposition of macromolecular coordinates resulting from crystallographic and NMR studies.
  Nat Struct Biol, 4, 173-174.  
9048206 B.J.Doranz, J.F.Berson, J.Rucker, and R.W.Doms (1997).
Chemokine receptors as fusion cofactors for human immunodeficiency virus type 1 (HIV-1).
  Immunol Res, 16, 15-28.  
9393669 G.McFadden, and D.Kelvin (1997).
New strategies for chemokine inhibition and modulation: you take the high road and I'll take the low road.
  Biochem Pharmacol, 54, 1271-1280.  
8989326 J.Lubkowski, G.Bujacz, L.Boqué, P.J.Domaille, T.M.Handel, and A.Wlodawer (1997).
The structure of MCP-1 in two crystal forms provides a rare example of variable quaternary interactions.
  Nat Struct Biol, 4, 64-69.
PDB codes: 1dok 1dol
8999852 K.Rajarathnam, C.M.Kay, B.Dewald, M.Wolf, M.Baggiolini, I.Clark-Lewis, and B.D.Sykes (1997).
Neutrophil-activating peptide-2 and melanoma growth-stimulatory activity are functional as monomers for neutrophil activation.
  J Biol Chem, 272, 1725-1729.  
9143704 M.Baggiolini, B.Dewald, and B.Moser (1997).
Human chemokines: an update.
  Annu Rev Immunol, 15, 675-705.  
9109648 S.Meunier, J.M.Bernassau, J.C.Guillemot, P.Ferrara, and H.Darbon (1997).
Determination of the three-dimensional structure of CC chemokine monocyte chemoattractant protein 3 by 1H two-dimensional NMR spectroscopy.
  Biochemistry, 36, 4412-4422.
PDB code: 1ncv
8662890 A.Meyer, A.J.Coyle, A.E.Proudfoot, T.N.Wells, and C.A.Power (1996).
Cloning and characterization of a novel murine macrophage inflammatory protein-1 alpha receptor.
  J Biol Chem, 271, 14445-14451.  
8639605 T.M.Handel, and P.J.Domaille (1996).
Heteronuclear (1H, 13C, 15N) NMR assignments and solution structure of the monocyte chemoattractant protein-1 (MCP-1) dimer.
  Biochemistry, 35, 6569-6584.
PDB codes: 1dom 1don
8906232 T.N.Wells, M.Lusti-Narasimhan, C.W.Chung, R.Cooke, C.A.Power, M.C.Peitsch, and A.E.Proudfoot (1996).
The molecular basis of selectivity between CC and CXC chemokines: the possibility of chemokine antagonists as anti-inflammatory agents.
  Ann N Y Acad Sci, 796, 245-256.  
8639560 W.J.Metzler, B.Leiting, K.Pryor, L.Mueller, and B.T.Farmer (1996).
The three-dimensional solution structure of the SH2 domain from p55blk kinase.
  Biochemistry, 35, 6201-6211.
PDB codes: 1blj 1blk
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.