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Structural protein
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PDB id
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1hqz
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Contents |
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(+ 1 more)
139 a.a.
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132 a.a.
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* Residue conservation analysis
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PDB id:
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Structural protein
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Title:
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Cofilin homology domain of a yeast actin-binding protein abp
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Structure:
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Actin-binding protein. Chain: 1, 2, 3, 4, 5, 6, 7, 8, 9. Fragment: n-terminal domain, residues 1-141. Synonym: abp1p. Engineered: yes
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Source:
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Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 562
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Resolution:
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2.10Å
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R-factor:
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0.213
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R-free:
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0.259
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Authors:
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B.V.Strokopytov,A.A.Fedorov,N.Mahoney,D.G.Drubin,S.C.Almo,S. New York Sgx Research Center For Structural Genomics (Nysgx
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Key ref:
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B.V.Strokopytov
et al.
(2005).
Phased translation function revisited: structure solution of the cofilin-homology domain from yeast actin-binding protein 1 using six-dimensional searches.
Acta Crystallogr D Biol Crystallogr,
61,
285-293.
PubMed id:
DOI:
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Date:
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20-Dec-00
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Release date:
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21-Dec-01
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PROCHECK
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Headers
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References
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Gene Ontology (GO) functional annotation
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Cellular component
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intracellular
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1 term
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Biochemical function
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actin binding
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1 term
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DOI no:
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Acta Crystallogr D Biol Crystallogr
61:285-293
(2005)
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PubMed id:
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Phased translation function revisited: structure solution of the cofilin-homology domain from yeast actin-binding protein 1 using six-dimensional searches.
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B.V.Strokopytov,
A.Fedorov,
N.M.Mahoney,
M.Kessels,
D.G.Drubin,
S.C.Almo.
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ABSTRACT
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A modified molecular-replacement method is described that makes use of
six-dimensional searches and the phased translation function, providing a
systematic examination of all possible search-model orientations in an
experimental electron-density map. As an example, the structure solution of the
cofilin-homology domain of the Saccharomyces cerevisiae actin-binding protein 1
(ABP1) is presented in detail. Additional examples are presented in which these
tools have significantly aided structure solutions in a variety of contexts.
These results suggest that this approach might be of widespread utility for
challenging structures involving weak phase information, complex asymmetric
units and search models with weak structural homology. Furthermore, this
approach supports an exhaustive molecular-replacement strategy in cases where an
appropriate search model cannot readily be identified on the basis of sequence
homology. The fully automated web-based implementation of this phased
translation function is described.
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Selected figure(s)
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Figure 4.
Figure 4
Averaged electron density at 2.1 Å resolution. A part of the CHD central [130][beta]
-sheet is shown formed by residues 32-37, 43-49, 65-71 and 80-86.
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Figure 5.
Figure 5
Superimposed structures of cofilin (in yellow) and ABP1p CHD (in green) shown as C^
[alpha] traces.
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The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(2005,
61,
285-293)
copyright 2005.
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Figures were
selected
by an automated process.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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J.Pfaendtner,
E.M.De La Cruz,
and
G.A.Voth
(2010).
Actin filament remodeling by actin depolymerization factor/cofilin.
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Proc Natl Acad Sci U S A, 107,
7299-7304.
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L.F.Westblade,
E.A.Campbell,
C.Pukhrambam,
J.C.Padovan,
B.E.Nickels,
V.Lamour,
and
S.A.Darst
(2010).
Structural basis for the bacterial transcription-repair coupling factor/RNA polymerase interaction.
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Nucleic Acids Res, 38,
8357-8369.
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PDB code:
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S.G.Brohawn,
and
T.U.Schwartz
(2009).
Molecular architecture of the Nup84-Nup145C-Sec13 edge element in the nuclear pore complex lattice.
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Nat Struct Mol Biol, 16,
1173-1177.
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PDB codes:
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B.A.Manjasetty,
A.P.Turnbull,
S.Panjikar,
K.Büssow,
and
M.R.Chance
(2008).
Automated technologies and novel techniques to accelerate protein crystallography for structural genomics.
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Proteomics, 8,
612-625.
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C.Bagnéris,
A.V.Ageichik,
N.Cronin,
B.Wallace,
M.Collins,
C.Boshoff,
G.Waksman,
and
T.Barrett
(2008).
Crystal structure of a vFlip-IKKgamma complex: insights into viral activation of the IKK signalosome.
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Mol Cell, 30,
620-631.
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PDB code:
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G.Wang,
M.G.Klein,
E.Tokonzaba,
Y.Zhang,
L.G.Holden,
and
X.S.Chen
(2008).
The structure of a DnaB-family replicative helicase and its interactions with primase.
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Nat Struct Mol Biol, 15,
94.
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PDB codes:
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H.S.Yin,
X.Wen,
R.G.Paterson,
R.A.Lamb,
and
T.S.Jardetzky
(2006).
Structure of the parainfluenza virus 5 F protein in its metastable, prefusion conformation.
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Nature, 439,
38-44.
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PDB code:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
