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Serine protease inhibitor
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PDB id
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1hpt
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Contents |
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* Residue conservation analysis
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Gene Ontology (GO) functional annotation
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Cellular component
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extracellular region
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1 term
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Biochemical function
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protein binding
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4 terms
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DOI no:
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J Mol Biol
225:1095-1103
(1992)
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PubMed id:
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Three-dimensional structure of a recombinant variant of human pancreatic secretory trypsin inhibitor (Kazal type).
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H.J.Hecht,
M.Szardenings,
J.Collins,
D.Schomburg.
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ABSTRACT
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A modified version of the human pancreatic trypsin inhibitor (PSTI), generated
in a protein-design project, has been crystallized in spacegroup P4(3) with
lattice constants a = 40.15 A, c = 33.91 A. The structure has been solved by
molecular replacement. Refinement of the structure by simulated annealing and
conventional restrained least-squares yielded for 8.0 to 2.3 A data a final
R-value of 19.1%. Differences to the known structures of porcine PSTI complexed
with trypsinogen and modified human PSTI complexed with chymotrypsinogen occur
at the flexible N-terminal part of the molecule. These differences are
influenced by crystal packing, as are low temperature factors for the binding
loop. The geometry of the binding loop is similar to the complexed structures.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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S.Chaudhury,
S.Lyskov,
and
J.J.Gray
(2010).
PyRosetta: a script-based interface for implementing molecular modeling algorithms using Rosetta.
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Bioinformatics, 26,
689-691.
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O.Király,
T.Wartmann,
and
M.Sahin-Tóth
(2007).
Missense mutations in pancreatic secretory trypsin inhibitor (SPINK1) cause intracellular retention and degradation.
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Gut, 56,
1433-1438.
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C.W.Luo,
H.J.Lin,
S.C.Gopinath,
and
Y.H.Chen
(2004).
Distinction of sperm-binding site and reactive site for trypsin inhibition on p12 secreted from the accessory sex glands of male mice.
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Biol Reprod, 70,
965-971.
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S.M.Lu,
W.Lu,
M.A.Qasim,
S.Anderson,
I.Apostol,
W.Ardelt,
T.Bigler,
Y.W.Chiang,
J.Cook,
M.N.James,
I.Kato,
C.Kelly,
W.Kohr,
T.Komiyama,
T.Y.Lin,
M.Ogawa,
J.Otlewski,
S.J.Park,
S.Qasim,
M.Ranjbar,
M.Tashiro,
N.Warne,
H.Whatley,
A.Wieczorek,
M.Wieczorek,
T.Wilusz,
R.Wynn,
W.Zhang,
and
M.Laskowski
(2001).
Predicting the reactivity of proteins from their sequence alone: Kazal family of protein inhibitors of serine proteinases.
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Proc Natl Acad Sci U S A, 98,
1410-1415.
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D.W.Ritchie,
and
G.J.Kemp
(2000).
Protein docking using spherical polar Fourier correlations.
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Proteins, 39,
178-194.
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R.Najmanovich,
J.Kuttner,
V.Sobolev,
and
M.Edelman
(2000).
Side-chain flexibility in proteins upon ligand binding.
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Proteins, 39,
261-268.
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G.Moont,
H.A.Gabb,
and
M.J.Sternberg
(1999).
Use of pair potentials across protein interfaces in screening predicted docked complexes.
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Proteins, 35,
364-373.
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J.Polanowska,
I.Krokoszynska,
H.Czapinska,
W.Watorek,
M.Dadlez,
and
J.Otlewski
(1998).
Specificity of human cathepsin G.
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Biochim Biophys Acta, 1386,
189-198.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
