PDBsum entry: 1hja

Complex (hydrolase/inhibitor)

PDB-id: 1hja

Asymmetric unit

Contents

Description

Header details

Header records

References

PROCHECK

Protein chains

131 a.a. *

97 a.a. *

51 a.a. *

Ligands

CYS-GLY-VAL-PRO-ALA-ILE-GLN-PRO-VAL-LEU

Waters ×99

* Residue conservation analysis

Tools

Clefts Calculation

Biological unit*, tetramer
(*as deduced by PQS)

PDB id:

1hja

Name:

Complex (hydrolase/inhibitor)

Title:

Lys 18 variant of turkey ovomucoid inhibitor third domain complexed with alpha-chymotrypsin

Structure:

Alpha-chymotrypsin. Chain: a. Alpha-chymotrypsin. Chain: b. Engineered: yes. Alpha-chymotrypsin. Chain: c. Engineered: yes. Ovomucoid inhibitor.

Source:

Bos taurus. Cattle. Organism_taxid: 9913. Other_details: commercial product of worthington biochemical corporation. Expressed in: escherichia coli. Expression_system_taxid: 562. Meleagris gallopavo. Turkey.

Biological unit:

Tetramer (from PQS)

UniProt:

Chain B: P00766 (CTRA_BOVIN)

Seq:	245 a.a.
Struc:	131 a.a.

Chain C: P00766 (CTRA_BOVIN)

Seq:	245 a.a.
Struc:	97 a.a.

Chain I: P68390 (IOVO_MELGA)

Seq:

185 a.a.

Struc:

51 a.a.*

Key:	PfamA domain
Secondary structure	CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme class:

Chains B, C: E.C.3.4.21.1   [IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

Reaction:

Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.

Resolution:

2.30Å

R-factor:

0.193

R-free:

0.246

Authors:

J.-H.Ding,M.N.G.James

Key ref:

j.ding et al. Crystal Structure of Lys18 Variant of Turkey Ovomucoid Inhibitor Third Domain Complexed with Alpha-Chymotrypsin at 2.3 A. To be Published, .

Date:

09-Jul-97

Release date:

14-Jan-98

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