 |
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Hydrolase
|
|
|
Title:
|
|
Arh-i, an angiogenin/rnase a chimera
|
|
Structure:
|
|
Angiogenin. Chain: a. Engineered: yes. Mutation: yes
|
|
Source:
|
|
Homo sapiens, bos taurus. Human, bovine. Organism_taxid: 9606, 9913. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: substituted sequence is bovine. Synthetic gene
|
|
Resolution:
|
|
|
2.10Å
|
R-factor:
|
0.220
|
R-free:
|
0.274
|
|
|
Authors:
|
|
D.E.Holloway,R.Shapiro,M.C.Hares,D.D.Leonidas,K.R.Acharya
|
Key ref:
|
|
D.E.Holloway
et al.
(2002).
Guest-host crosstalk in an angiogenin-RNase A chimeric protein.
Biochemistry,
41,
10482-10489.
PubMed id:
DOI:
|
|
|
Date:
|
|
|
06-Feb-02
|
Release date:
|
22-Aug-02
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
|
|
|
|
Enzyme class:
|
|
E.C.3.1.27.5
- Pancreatic ribonuclease.
|
|
|
|
|
|
|
Reaction:
|
|
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in C-P or U-P with 2',3'-cyclic phosphate intermediates.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Gene Ontology (GO) functional annotation
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Cellular component
|
extracellular region
|
8 terms
|
|
|
Biological process
|
metabolic process
|
24 terms
|
|
|
Biochemical function
|
nucleic acid binding
|
14 terms
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
DOI no:
|
Biochemistry
41:10482-10489
(2002)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Guest-host crosstalk in an angiogenin-RNase A chimeric protein.
|
|
D.E.Holloway,
R.Shapiro,
M.C.Hares,
D.D.Leonidas,
K.R.Acharya.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
Angiogenin and ribonuclease A share 33% sequence identity but have distinct
functions. Angiogenin is a potent inducer of angiogenesis that is only weakly
ribonucleolytic, whereas ribonuclease A is a robust ribonuclease that is not
angiogenic. A chimera ("ARH-I"), in which angiogenin residues 58-70
are replaced with residues 59-73 of ribonuclease A, has intermediate
ribonucleolytic potency and no angiogenic activity. Here we report a crystal
structure of ARH-I that reveals the molecular basis for these characteristics.
The ribonuclease A-derived (guest) segment adopts a structure largely similar to
that in ribonuclease A, and successfully converts this region from a
cell-binding site to a purine-binding site. At the same time, its presence
causes complex changes in the angiogenin-derived (host) portion that account for
much of the increased ribonuclease activity of ARH-I. Guest-host interactions of
this type probably occur more generally in protein chimeras, emphasizing the
importance of direct structural information for understanding the functional
behavior of such molecules.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Literature references that cite this PDB file's key reference
|
|
|
| |
PubMed id
|
|
Reference
|
|
|
|
|
|
R.Tomecki,
and
A.Dziembowski
(2010).
Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism.
|
| |
RNA, 16,
1692-1724.
|
|
|
|
|
|
|
W.M.Li,
T.Barnes,
and
C.H.Lee
(2010).
Endoribonucleases--enzymes gaining spotlight in mRNA metabolism.
|
| |
FEBS J, 277,
627-641.
|
|
|
|
|
|
|
K.Kazakou,
D.E.Holloway,
S.H.Prior,
V.Subramanian,
and
K.R.Acharya
(2008).
Ribonuclease A homologues of the zebrafish: polymorphism, crystal structures of two representatives and their evolutionary implications.
|
| |
J Mol Biol, 380,
206-222.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
S.Wang,
and
H.Li
(2006).
Radical scavenging activity of ribonuclease inhibitor from cow placenta.
|
| |
Biochemistry (Mosc), 71,
520-524.
|
|
|
|
|
|
|
D.E.Holloway,
G.B.Chavali,
M.C.Hares,
V.Subramanian,
and
K.R.Acharya
(2005).
Structure of murine angiogenin: features of the substrate- and cell-binding regions and prospects for inhibitor-binding studies.
|
| |
Acta Crystallogr D Biol Crystallogr, 61,
1568-1578.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
T.Wang,
M.Yang,
J.Chen,
T.Watkins,
and
C.Xiuyun
(2005).
Inhibition of B16 melanoma growth in vivo by retroviral vector-mediated human ribonuclease inhibitor.
|
| |
Angiogenesis, 8,
73-81.
|
|
|
|
|
|
|
X.Liu,
and
E.C.Theil
(2004).
Ferritin reactions: direct identification of the site for the diferric peroxide reaction intermediate.
|
| |
Proc Natl Acad Sci U S A, 101,
8557-8562.
|
|
|
|
|
|
The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
|
| |
Links
