PDBsum entry 1gsc

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protein links
Transferase(glutathione) PDB id
Protein chains
217 a.a.* *
* Residue conservation analysis
* C-alpha coords only
PDB id:
Name: Transferase(glutathione)
Title: New crystal forms of a mu class glutathione s-transferase from rat liver
Structure: Glutathione s-transferase. Chain: a, b, c, d. Engineered: yes
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: liver
Biol. unit: Dimer (from PQS)
2.50Å     R-factor:   not given    
Authors: J.-H.Fu,J.P.Rose,B.-C.Wang
Key ref:
J.H.Fu et al. (1994). New crystal forms of a micro-class glutathione S-transferase from rat liver. Acta Crystallogr D Biol Crystallogr, 50, 219-224. PubMed id: 15299462 DOI: 10.1107/S0907444993009370
08-Sep-93     Release date:   31-Oct-93    

Protein chains
Pfam   ArchSchema ?
P04905  (GSTM1_RAT) -  Glutathione S-transferase Mu 1
218 a.a.
217 a.a.
Key:    PfamA domain  Secondary structure

 Enzyme reactions 
   Enzyme class: E.C.  - Glutathione transferase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: RX + glutathione = HX + R-S-glutathione
+ glutathione
= HX
+ R-S-glutathione
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   3 terms 
  Biological process     metabolic process   12 terms 
  Biochemical function     transferase activity     5 terms  


DOI no: 10.1107/S0907444993009370 Acta Crystallogr D Biol Crystallogr 50:219-224 (1994)
PubMed id: 15299462  
New crystal forms of a micro-class glutathione S-transferase from rat liver.
J.H.Fu, J.Rose, M.F.Tam, B.C.Wang.
Two new crystal forms of isoenzyme 3-3 of rat liver glutathione S-transferase (GST 3-3) have been obtained. They were grown under essentially the same crystallization conditions as those reported for the C2 crystal form [Fu, Rose, Chung, Tam & Wang (1991). Acta Cryst. B47, 813-814]. The new crystals belong to space group P2(1) with one form having cell dimensions a = 101.6, b = 69.5, c = 81.4 A, and beta = 113.6 degrees, and the other form having cell parameters a = 97.4, b = 81.1, c = 69.4 A and beta = 109.2 degrees. These new crystals diffract to at least 2.5 A, resolution. The molecular packing arrangements in these P2(1) crystals have been found by molecular replacement studies.
  Selected figure(s)  
Figure 1.
Fig. 1. Multiple crystal forms of rat liver GST 3-3, grown under the same conditions: 20% PEG 3350, 10 mM fl-OG, 0.6 mM EtHgC1, 100 mM Hepes, pH 8.0.
Figure 2.
Fig. 2. The self-rotation functions, K = 180~: (a) form B crystals of GST 3-3; (b) form C crystals of GST 3-3. The b axes are perpendicular to the paper. For the calculation, data to 5 A were used with a Patterson radius of 30/k.
  The above figures are reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (1994, 50, 219-224) copyright 1994.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
11970949 F.E.Chen-Park, D.B.Huang, B.Noro, D.Thanos, and G.Ghosh (2002).
The kappa B DNA sequence from the HIV long terminal repeat functions as an allosteric regulator of HIV transcription.
  J Biol Chem, 277, 24701-24708.
PDB code: 1lei
10570132 J.M.van Den Elsen, D.A.Kuntz, F.J.Hoedemaeker, and D.R.Rose (1999).
Antibody C219 recognizes an alpha-helical epitope on P-glycoprotein.
  Proc Natl Acad Sci U S A, 96, 13679-13684.
PDB code: 2ap2
7892174 K.Zeng, J.P.Rose, H.C.Chen, C.L.Strickland, C.P.Tu, and B.C.Wang (1994).
A surface mutant (G82R) of a human alpha-glutathione S-transferase shows decreased thermal stability and a new mode of molecular association in the crystal.
  Proteins, 20, 259-263.
PDB code: 1ags
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