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Hydrolase angiogenesis PDB id
1gio
Jmol
Contents
Protein chain
125 a.a. *
* Residue conservation analysis
PDB id:
1gio
Name: Hydrolase angiogenesis
Title: Nmr solution structure of bovine angiogenin, 10 structures
Structure: Angiogenin. Chain: a. Ec: 3.1.27.-
Source: Bos taurus. Cattle. Organism_taxid: 9913. Secretion: milk
NMR struc: 10 models
Authors: O.Lequin,C.Albaret,F.Bontems,G.Spik,J.Y.Lallemand
Key ref:
O.Lequin et al. (1996). Solution structure of bovine angiogenin by 1H nuclear magnetic resonance spectroscopy. Biochemistry, 35, 8870-8880. PubMed id: 8688423 DOI: 10.1021/bi960022r
Date:
12-Apr-96     Release date:   07-Dec-96    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P10152  (ANG1_BOVIN) -  Angiogenin-1
Seq:
Struc: 		148 a.a.
125 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   8 terms 
  Biological process     multicellular organismal development   14 terms 
  Biochemical function     nucleic acid binding     11 terms  

 

 
DOI no: 10.1021/bi960022r Biochemistry 35:8870-8880 (1996)
PubMed id: 8688423  
 
 
Solution structure of bovine angiogenin by 1H nuclear magnetic resonance spectroscopy.
O.Lequin, C.Albaret, F.Bontems, G.Spik, J.Y.Lallemand.
 
  ABSTRACT  
 
The three-dimensional structure of bovine angiogenin has been determined using two- and three-dimensional proton NMR spectroscopy. The solution structure is very close to that recently determined by X-ray diffraction analysis. This structure appears well defined, even if five loops and one helix exhibit greater flexibility. Analysis of the active site geometry confirms the position of the Glu-118 residue which obstructs the pyrimidine binding site. There is no experimental evidence of an unobstructed conformation of angiogenin in solution. In addition, it appears that the Glu-118 and Ser-119 residues and the cell receptor binding loop may play an important role in the differences of C-terminal fragment organization and ribonucleolytic activity observed between angiogenins and ribonuclease A.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
11093266 M.S.Madhusudhan, and S.Vishveshwara (2001).
Computer modeling of human angiogenin-dinucleotide substrate interaction.
  Proteins, 42, 125-135.  
10070263 M.S.Madhusudhan, and S.Vishveshwara (1999).
Comparison of the dynamics of bovine and human angiogenin: a molecular dynamics study.
  Biopolymers, 49, 131-144.  
9578571 R.Shapiro (1998).
Structural features that determine the enzymatic potency and specificity of human angiogenin: threonine-80 and residues 58-70 and 116-123.
  Biochemistry, 37, 6847-6856.  
9181574 C.H.Schein (1997).
From housekeeper to microsurgeon: the diagnostic and therapeutic potential of ribonucleases.
  Nat Biotechnol, 15, 529-536.  
9461294 O.Lequin, H.Thüring, M.Robin, and J.Y.Lallemand (1997).
Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values.
  Eur J Biochem, 250, 712-726.
PDB code: 1awz
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.