PDBsum entry: 1f4s

Transcription/DNA

PDB id: 1f4s

Contents

Protein chain

65 a.a. *

DNA/RNA

Metals

_ZN ×2

Waters ×3

* Residue conservation analysis

PDB id:

1f4s

Name:

Transcription/DNA

Title:

Structure of transcriptional factor alcr in complex with a target DNA

Structure:

DNA (5'-d(p Cp Gp Tp Gp Cp Gp Gp Ap Tp C)-3'). Chain: a. Engineered: yes. Other_details: alcr consensus half-target. DNA (5'-d(p Gp Ap Tp Cp Cp Gp Cp Ap Cp G)-3'). Chain: b. Engineered: yes. Other_details: alcr consensus half-target. Ethanol regulon transcriptional factor.

Source:

Synthetic: yes. Emericella nidulans. Organism_taxid: 162425. Expressed in: escherichia coli. Expression_system_taxid: 562

NMR struc:

10 models

Authors:

B.Cahuzac,R.Cerdan,B.Felenbok,E.Guittet

Key ref:

B.Cahuzac et al. (2001). The solution structure of an AlcR-DNA complex sheds light onto the unique tight and monomeric DNA binding of a Zn(2)Cys(6) protein. Structure, 9, 827-836. PubMed id: 11566132 DOI: 10.1016/S0969-2126(01)00640-2

Date:

09-Jun-00 Release date: 28-Sep-01

Protein chain

P21228  (ALCR_EMENI) -  Regulatory protein alcR

Seq: 821 a.a.

Struc: 65 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 

• Cellular component: nucleus (1 term)
• Biological process: regulation of transcription, DNA-dependent (1 term)
• Biochemical function: transcription factor activity (2 terms)

DOI no: 10.1016/S0969-2126(01)00640-2

Structure 9:827-836 (2001)

PubMed id: 11566132

The solution structure of an AlcR-DNA complex sheds light onto the unique tight and monomeric DNA binding of a Zn(2)Cys(6) protein.

B.Cahuzac, R.Cerdan, B.Felenbok, E.Guittet.

ABSTRACT

BACKGROUND: In Aspergillus nidulans, the transcription activator AlcR mediates specific induction of a number of the genes of the alc cluster. This cluster includes genes involved in the oxidation of ethanol and other alcohols to acetate. The pattern of binding and of transactivation of AlcR is unique within the Zn(2)Cys(6) family. The structural bases for these specificities have not been analyzed at the atomic level until now. RESULTS: We have used NMR spectroscopy and restrained molecular dynamics to determine a set of structures of the AlcR DNA binding domain [AlcR(1-60)] in complex with a 10-mer DNA duplex. Analysis of the structures reveals specific interactions between AlcR and DNA common to the other known zinc clusters. In addition, the involvement of the N-terminal residues upstream of the AlcR zinc cluster in DNA binding is clearly highlighted, and the pivotal role of R6 is confirmed. Totally unprecedented specific and nonspecific contacts of two additional regions of the protein with the DNA are demonstrated. The differences with the available crystallographic structures of other zinc binuclear cluster proteins-DNA complexes are analyzed. CONCLUSIONS: The structures of the AlcR(1-60)-DNA complex provide the basis for a better understanding of some of the specificities of the AlcR system: the DNA consensus recognition sequence--usually the triplet CGG--is extended to five base pairs, AlcR acts as a monomer, and additional contacts inside and outside the DNA binding domain in the major and minor groove are observed. These extensive interactions stabilize the AlcR monomer to its cognate DNA site.

Selected figure(s)

Figure 3.
Figure 3. Schematic Representation of Some of the Protein-DNA Contacts Observed in the AlcR-DNA ComplexSequence-specific contacts are shown with plain arrows and dashed arrows for the major and minor groove, respectively. Hydrophobic contacts involving W45 are also depicted with plain arrows. Nonspecific interactions with the sugar phosphate backbone are shown in black

The above figure is reprinted by permission from Cell Press: Structure (2001, 9, 827-836) copyright 2001.

Figure was selected by the author.