PDBsum entry: 1f2m

Hydrolase

PDB-id: 1f2m

Contents

Description

Header details

Header records

References

PROCHECK

Protein chain

136 a.a. *

Waters ×58

* Residue conservation analysis

Tools

Clefts Calculation

PDB id:

1f2m

Name:

Hydrolase

Title:

Simplification of a protein loop in staphylococcal nuclease

Structure:

Staphylococcal nuclease. Chain: a. Engineered: yes. Mutation: yes

Source:

Staphylococcus aureus. Organism_taxid: 1280. Strain: foggi. Expressed in: escherichia coli. Expression_system_taxid: 562.

UniProt:

P00644 (NUC_STAAU)

Seq:

231 a.a.

Struc:

136 a.a.*

Key:	PfamA domain	PfamB domain
Secondary structure	CATH domain

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme class:

E.C.3.1.31.1   [IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

Reaction:

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

Resolution:

2.00Å

R-factor:

0.195

Authors:

A.Hodel,P.C.Harkins,D.M.Adelman,M.A.White,R.O.Fox

Key ref:

a.hodel et al. Simplification of a Protein Loop in Staphylococcal Nuclease. To be Published, .

Date:

26-May-00

Release date:

16-Sep-03

Related entries:

1stn
wild-type protein
1eqv
part of same study
1f2y
1f2z

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Clefts

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