PDBsum entry 1ezv

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protein ligands Protein-protein interface(s) links
Oxidoreductase/electron transport PDB id
Protein chains
430 a.a. *
352 a.a. *
385 a.a. *
245 a.a. *
185 a.a. *
74 a.a. *
125 a.a. *
93 a.a. *
55 a.a. *
127 a.a. *
107 a.a. *
HEM ×3
Waters ×346
* Residue conservation analysis
PDB id:
Name: Oxidoreductase/electron transport
Title: Structure of the yeast cytochrome bc1 complex co- crystallized with an antibody fv-fragment
Structure: Ubiquinol-cytochromE C reductase complex core protein i. Chain: a. Fragment: residues 24-457. Engineered: yes. Ubiquinol-cytochromE C reductase complex core protein 2. Chain: b. Fragment: residues 17-368.
Source: Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Organelle: mitochondria. Other_details: mitochondria, yeast, saccharomyces cerevisiae. Other_details: fv-fragment derived from the murine monoclonal antibody 18e11, expression system escherichia coli.
Biol. unit: 22mer (from PDB file)
2.30Å     R-factor:   0.222     R-free:   0.254
Authors: C.Hunte,J.Koepke,C.Lange,T.Rossmanith,H.Michel
Key ref:
C.Hunte et al. (2000). Structure at 2.3 A resolution of the cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment. Structure, 8, 669-684. PubMed id: 10873857
12-May-00     Release date:   16-May-01    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
P07256  (QCR1_YEAST) -  Cytochrome b-c1 complex subunit 1, mitochondrial
457 a.a.
430 a.a.*
Protein chain
Pfam   ArchSchema ?
P07257  (QCR2_YEAST) -  Cytochrome b-c1 complex subunit 2, mitochondrial
368 a.a.
352 a.a.
Protein chain
Pfam   ArchSchema ?
P00163  (CYB_YEAST) -  Cytochrome b
385 a.a.
385 a.a.*
Protein chain
Pfam   ArchSchema ?
P07143  (CY1_YEAST) -  Cytochrome c1, heme protein, mitochondrial
309 a.a.
245 a.a.
Protein chain
Pfam   ArchSchema ?
P08067  (UCRI_YEAST) -  Cytochrome b-c1 complex subunit Rieske, mitochondrial
215 a.a.
185 a.a.
Protein chain
Pfam   ArchSchema ?
P00127  (QCR6_YEAST) -  Cytochrome b-c1 complex subunit 6
147 a.a.
74 a.a.
Protein chain
Pfam   ArchSchema ?
P00128  (QCR7_YEAST) -  Cytochrome b-c1 complex subunit 7
127 a.a.
125 a.a.
Protein chain
Pfam   ArchSchema ?
P08525  (QCR8_YEAST) -  Cytochrome b-c1 complex subunit 8
94 a.a.
93 a.a.
Protein chain
Pfam   ArchSchema ?
P22289  (QCR9_YEAST) -  Cytochrome b-c1 complex subunit 9
66 a.a.
55 a.a.
Protein chain
Pfam   ArchSchema ?
P18531  (HVM60_MOUSE) -  Ig heavy chain V region 3-6
116 a.a.
127 a.a.*
Protein chain
Pfam   ArchSchema ?
P01647  (KV5AE_MOUSE) -  Ig kappa chain V-V region HP 124E1
108 a.a.
107 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 29 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chain E: E.C.  - Quinol--cytochrome-c reductase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+
Bound ligand (Het Group name = UQ6)
matches with 53.00% similarity
2 × ferricytochrome c
Bound ligand (Het Group name = HEM)
matches with 63.00% similarity
= quinone
+ 2 × ferrocytochrome c
+ 2 × H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   8 terms 
  Biological process     oxidation-reduction process   7 terms 
  Biochemical function     catalytic activity     13 terms  


Structure 8:669-684 (2000)
PubMed id: 10873857  
Structure at 2.3 A resolution of the cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment.
C.Hunte, J.Koepke, C.Lange, T.Rossmanith, H.Michel.
BACKGROUND: The cytochrome bc(1) complex is part of the energy conversion machinery of the respiratory and photosynthetic electron transfer chains. This integral membrane protein complex catalyzes electron transfer from ubiquinol to cytochrome c. It couples the electron transfer to the electrogenic translocation of protons across the membrane via a so-called Q cycle mechanism. RESULTS: The cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae was crystallized together with a bound antibody Fv fragment. The structure was determined at 2.3 A resolution using multiple isomorphous replacement, and refined to a crystallographic R factor of 22.2% (R(free) = 25.4%). The complex is present as a homodimer. Each 'monomer' of the refined model includes 2178 amino acid residues of subunits COR1, QCR2, COB, CYT1, RIP1, QCR6, QCR7, QCR8 and QCR9 of the cytochrome bc(1) complex and of the polypeptides V(H) and V(L) of the Fv fragment, the cofactors heme b(H), heme b(L), heme c(1), the [2Fe-2S] cluster and 346 water molecules. The Fv fragment binds to the extrinsic domain of the [2Fe-2S] Rieske protein and is essential for formation of the crystal lattice. CONCLUSIONS: The approach to crystallize membrane proteins as complexes with specific antibody fragments appears to be of general importance. The structure of the yeast cytochrome bc(1) complex reveals in detail the binding sites of the natural substrate coenzyme Q6 and the inhibitor stigmatellin. Buried water molecules close to the binding sites suggest possible pathways for proton uptake and release. A comparison with other cytochrome bc(1) complexes shows features that are specific to yeast.

Literature references that cite this PDB file's key reference

  PubMed id Reference
21168530 L.Mathieu, S.Marsy, Y.Saint-Georges, C.Jacq, and G.Dujardin (2011).
A transcriptome screen in yeast identifies a novel assembly factor for the mitochondrial complex III.
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20814880 C.X.Luo, M.J.Hu, X.Jin, L.F.Yin, P.K.Bryson, and G.Schnabel (2010).
An intron in the cytochrome b gene of Monilinia fructicola mitigates the risk of resistance development to QoI fungicides.
  Pest Manag Sci, 66, 1308-1315.  
20025846 E.A.Berry, L.S.Huang, D.W.Lee, F.Daldal, K.Nagai, and N.Minagawa (2010).
Ascochlorin is a novel, specific inhibitor of the mitochondrial cytochrome bc1 complex.
  Biochim Biophys Acta, 1797, 360-370.
PDB code: 3h1l
19739941 G.Lenaz, and M.L.Genova (2010).
Structure and organization of mitochondrial respiratory complexes: a new understanding of an old subject.
  Antioxid Redox Signal, 12, 961.  
20496909 K.L.Hsueh, W.M.Westler, and J.L.Markley (2010).
NMR investigations of the Rieske protein from Thermus thermophilus support a coupled proton and electron transfer mechanism.
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19826804 K.McLuskey, A.W.Roszak, Y.Zhu, and N.W.Isaacs (2010).
Crystal structures of all-alpha type membrane proteins.
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20667175 K.R.Vinothkumar, and R.Henderson (2010).
Structures of membrane proteins.
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19660431 L.M.Hughes, R.Covian, G.W.Gribble, and B.L.Trumpower (2010).
Probing binding determinants in center P of the cytochrome bc(1) complex using novel hydroxy-naphthoquinones.
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19892700 M.Castellani, R.Covian, T.Kleinschroth, O.Anderka, B.Ludwig, and B.L.Trumpower (2010).
Direct demonstration of half-of-the-sites reactivity in the dimeric cytochrome bc1 complex: enzyme with one inactive monomer is fully active but unable to activate the second ubiquinol oxidation site in response to ligand binding at the ubiquinone reduction site.
  J Biol Chem, 285, 502-510.  
19099453 D.R.Kolling, R.I.Samoilova, A.A.Shubin, A.R.Crofts, and S.A.Dikanov (2009).
Proton environment of reduced Rieske iron-sulfur cluster probed by two-dimensional ESEEM spectroscopy.
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19179332 M.G.Ding, J.P.di Rago, and B.L.Trumpower (2009).
Combining Inhibitor Resistance-conferring Mutations in Cytochrome b Creates Conditional Synthetic Lethality in Saccharomyces cerevisiae.
  J Biol Chem, 284, 8478-8485.  
19415898 M.Sarewicz, M.Dutka, W.Froncisz, and A.Osyczka (2009).
Magnetic interactions sense changes in distance between heme b(L) and the iron-sulfur cluster in cytochrome bc(1).
  Biochemistry, 48, 5708-5720.  
19176478 R.Covian, and B.L.Trumpower (2009).
Ilicicolin Inhibition and Binding at Center N of the Dimeric Cytochrome bc1 Complex Reveal Electron Transfer and Regulatory Interactions between Monomers.
  J Biol Chem, 284, 8614-8620.  
19325183 R.Covian, and B.L.Trumpower (2009).
The Rate-limiting Step in the Cytochrome bc1 Complex (Ubiquinol-Cytochrome c Oxidoreductase) Is Not Changed by Inhibition of Cytochrome b-dependent Deprotonation: IMPLICATIONS FOR THE MECHANISM OF UBIQUINOL OXIDATION AT CENTER P OF THE bc1 COMPLEX.
  J Biol Chem, 284, 14359-14367.  
19175316 R.E.Berry, M.N.Shokhirev, A.Y.Ho, F.Yang, T.K.Shokhireva, H.Zhang, A.Weichsel, W.R.Montfort, and F.A.Walker (2009).
Effect of mutation of carboxyl side-chain amino acids near the heme on the midpoint potentials and ligand binding constants of nitrophorin 2 and its NO, histamine, and imidazole complexes.
  J Am Chem Soc, 131, 2313-2327.
PDB code: 3fll
19369254 R.Pardossi-Piquard, S.P.Yang, S.Kanemoto, Y.Gu, F.Chen, C.Böhm, J.Sevalle, T.Li, P.C.Wong, F.Checler, G.Schmitt-Ulms, P.St George-Hyslop, and P.E.Fraser (2009).
APH1 Polar Transmembrane Residues Regulate the Assembly and Activity of Presenilin Complexes.
  J Biol Chem, 284, 16298-16307.  
19128449 S.Carmi, E.Y.Levanon, and E.Eisenberg (2009).
Efficiency of complex production in changing environment.
  BMC Syst Biol, 3, 3.  
19236481 V.Zara, L.Conte, and B.L.Trumpower (2009).
Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane.
  FEBS J, 276, 1900-1914.  
19503808 W.A.Beckstead, M.T.Ebbert, M.J.Rowe, and D.A.McClellan (2009).
Evolutionary pressure on mitochondrial cytochrome b is consistent with a role of CytbI7T affecting longevity during caloric restriction.
  PLoS One, 4, e5836.  
18501698 A.R.Crofts, J.T.Holland, D.Victoria, D.R.Kolling, S.A.Dikanov, R.Gilbreth, S.Lhee, R.Kuras, and M.G.Kuras (2008).
The Q-cycle reviewed: How well does a monomeric mechanism of the bc(1) complex account for the function of a dimeric complex?
  Biochim Biophys Acta, 1777, 1001-1019.  
18701458 B.Gurung, L.Yu, and C.A.Yu (2008).
Stigmatellin Induces Reduction of Iron-Sulfur Protein in the Oxidized Cytochrome bc1 Complex.
  J Biol Chem, 283, 28087-28094.  
18953640 D.Xia, L.Esser, M.Elberry, F.Zhou, L.Yu, and C.A.Yu (2008).
The road to the crystal structure of the cytochrome bc (1) complex from the anoxigenic, photosynthetic bacterium Rhodobacter sphaeroides.
  J Bioenerg Biomembr, 40, 485-492.  
18418633 E.A.Berry, and F.A.Walker (2008).
Bis-histidine-coordinated hemes in four-helix bundles: how the geometry of the bundle controls the axial imidazole plane orientations in transmembrane cytochromes of mitochondrial complexes II and III and related proteins.
  J Biol Inorg Chem, 13, 481-498.  
18674618 E.P.Carpenter, K.Beis, A.D.Cameron, and S.Iwata (2008).
Overcoming the challenges of membrane protein crystallography.
  Curr Opin Struct Biol, 18, 581-586.  
18022381 F.A.Rotsaert, M.G.Ding, and B.L.Trumpower (2008).
Differential efficacy of inhibition of mitochondrial and bacterial cytochrome bc1 complexes by center N inhibitors antimycin, ilicicolin H and funiculosin.
  Biochim Biophys Acta, 1777, 211-219.  
19006325 J.Janzon, Q.Yuan, F.Malatesta, P.Hellwig, B.Ludwig, B.Durham, and F.Millett (2008).
Probing the Paracoccus denitrificans cytochrome c(1)-cytochrome c(552) interaction by mutagenesis and fast kinetics.
  Biochemistry, 47, 12974-12984.  
18498758 M.G.Ding, C.A.Butler, S.A.Saracco, T.D.Fox, F.Godard, J.P.di Rago, and B.L.Trumpower (2008).
Introduction of cytochrome b mutations in Saccharomyces cerevisiae by a method that allows selection for both functional and non-functional cytochrome b proteins.
  Biochim Biophys Acta, 1777, 1147-1156.  
18093133 N.Fisher, and B.Meunier (2008).
Molecular basis of resistance to cytochrome bc1 inhibitors.
  FEMS Yeast Res, 8, 183-192.  
18703839 P.Lukat, M.Hoffmann, and O.Einsle (2008).
Crystal packing of the c(6)-type cytochrome OmcF from Geobacter sulfurreducens is mediated by an N-terminal Strep-tag II.
  Acta Crystallogr D Biol Crystallogr, 64, 919-926.
PDB code: 3dp5
18454936 R.Covian, and B.L.Trumpower (2008).
The dimeric structure of the cytochrome bc(1) complex prevents center P inhibition by reverse reactions at center N.
  Biochim Biophys Acta, 1777, 1044-1052.  
18471987 R.Covian, and B.L.Trumpower (2008).
Regulatory interactions in the dimeric cytochrome bc(1) complex: the advantages of being a twin.
  Biochim Biophys Acta, 1777, 1079-1091.  
18180306 S.L.Cuddihy, S.S.Ali, E.S.Musiek, J.Lucero, S.J.Kopp, J.D.Morrow, and L.L.Dugan (2008).
Prolonged alpha-tocopherol deficiency decreases oxidative stress and unmasks alpha-tocopherol-dependent regulation of mitochondrial function in the brain.
  J Biol Chem, 283, 6915-6924.  
18721136 T.Kleinschroth, O.Anderka, M.Ritter, A.Stocker, T.A.Link, B.Ludwig, and P.Hellwig (2008).
Characterization of mutations in crucial residues around the Q(o) binding site of the cytochrome bc complex from Paracoccus denitrificans.
  FEBS J, 275, 4773-4785.  
18445622 V.Tereshko, S.Uysal, A.Koide, K.Margalef, S.Koide, and A.A.Kossiakoff (2008).
Toward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold.
  Protein Sci, 17, 1175-1187.
PDB codes: 2p42 2p43 2p44 2p45 2p46 2p47 2p48
17200733 A.Y.Mulkidjanian (2007).
Proton translocation by the cytochrome bc1 complexes of phototrophic bacteria: introducing the activated Q-cycle.
  Photochem Photobiol Sci, 6, 19-34.  
17665226 B.N.Hudder, J.G.Morales, A.Stubna, E.Münck, M.P.Hendrich, and P.A.Lindahl (2007).
Electron paramagnetic resonance and Mössbauer spectroscopy of intact mitochondria from respiring Saccharomyces cerevisiae.
  J Biol Inorg Chem, 12, 1029-1053.  
17457691 D.Xia, L.Esser, L.Yu, and C.A.Yu (2007).
Structural basis for the mechanism of electron bifurcation at the quinol oxidation site of the cytochrome bc1 complex.
  Photosynth Res, 92, 17-34.  
17498743 E.Yamashita, H.Zhang, and W.A.Cramer (2007).
Structure of the cytochrome b6f complex: quinone analogue inhibitors as ligands of heme cn.
  J Mol Biol, 370, 39-52.
PDB codes: 2e74 2e75 2e76
17164236 G.D.Fairn, K.Macdonald, and C.R.McMaster (2007).
A chemogenomic screen in Saccharomyces cerevisiae uncovers a primary role for the mitochondria in farnesol toxicity and its regulation by the Pkc1 pathway.
  J Biol Chem, 282, 4868-4874.  
17383607 J.J.Kessl, N.V.Moskalev, G.W.Gribble, M.Nasr, S.R.Meshnick, and B.L.Trumpower (2007).
Parameters determining the relative efficacy of hydroxy-naphthoquinone inhibitors of the cytochrome bc1 complex.
  Biochim Biophys Acta, 1767, 319-326.  
17573435 L.Giachini, F.Francia, G.Veronesi, D.W.Lee, F.Daldal, L.S.Huang, E.A.Berry, T.Cocco, S.Papa, F.Boscherini, and G.Venturoli (2007).
X-Ray absorption studies of Zn2+ binding sites in bacterial, avian, and bovine cytochrome bc1 complexes.
  Biophys J, 93, 2934-2951.  
16963124 M.R.Jones (2007).
Lipids in photosynthetic reaction centres: structural roles and functional holes.
  Prog Lipid Res, 46, 56-87.  
17557793 N.Buzhynskyy, P.Sens, V.Prima, J.N.Sturgis, and S.Scheuring (2007).
Rows of ATP synthase dimers in native mitochondrial inner membranes.
  Biophys J, 93, 2870-2876.  
17544294 N.Morgner, T.Kleinschroth, H.D.Barth, B.Ludwig, and B.Brutschy (2007).
A novel approach to analyze membrane proteins by laser mass spectrometry: from protein subunits to the integral complex.
  J Am Soc Mass Spectrom, 18, 1429-1438.  
17561507 R.Covian, T.Kleinschroth, B.Ludwig, and B.L.Trumpower (2007).
Asymmetric binding of stigmatellin to the dimeric Paracoccus denitrificans bc1 complex: evidence for anti-cooperative ubiquinol oxidation and communication between center P ubiquinol oxidation sites.
  J Biol Chem, 282, 22289-22297.  
17616531 S.A.Dikanov, J.T.Holland, B.Endeward, D.R.Kolling, R.I.Samoilova, T.F.Prisner, and A.R.Crofts (2007).
Hydrogen bonds between nitrogen donors and the semiquinone in the Qi-site of the bc1 complex.
  J Biol Chem, 282, 25831-25841.  
17962407 S.Nauli, S.Farr, Y.J.Lee, H.Y.Kim, S.Faham, and J.U.Bowie (2007).
Polymer-driven crystallization.
  Protein Sci, 16, 2542-2551.
PDB codes: 2qar 2qb0 2qb1
17145759 T.Wenz, R.Covian, P.Hellwig, F.Macmillan, B.Meunier, B.L.Trumpower, and C.Hunte (2007).
Mutational analysis of cytochrome b at the ubiquinol oxidation site of yeast complex III.
  J Biol Chem, 282, 3977-3988.  
17399709 V.P.Shinkarev, and C.A.Wraight (2007).
Intermonomer electron transfer in the bc1 complex dimer is controlled by the energized state and by impaired electron transfer between low and high potential hemes.
  FEBS Lett, 581, 1535-1541.  
17680808 V.Zara, L.Conte, and B.L.Trumpower (2007).
Identification and characterization of cytochrome bc(1) subcomplexes in mitochondria from yeast with single and double deletions of genes encoding cytochrome bc(1) subunits.
  FEBS J, 274, 4526-4539.  
17534481 X.Liang, D.J.Campopiano, and P.J.Sadler (2007).
Metals in membranes.
  Chem Soc Rev, 36, 968-992.  
16829675 E.Maklashina, P.Hellwig, R.A.Rothery, V.Kotlyar, Y.Sher, J.H.Weiner, and G.Cecchini (2006).
Differences in protonation of ubiquinone and menaquinone in fumarate reductase from Escherichia coli.
  J Biol Chem, 281, 26655-26664.  
16551638 E.Schäfer, H.Seelert, N.H.Reifschneider, F.Krause, N.A.Dencher, and J.Vonck (2006).
Architecture of active mammalian respiratory chain supercomplexes.
  J Biol Chem, 281, 15370-15375.  
16586113 F.A.Walker (2006).
The heme environment of mouse neuroglobin: histidine imidazole plane orientations obtained from solution NMR and EPR spectroscopy as compared with X-ray crystallography.
  J Biol Inorg Chem, 11, 391-397.  
16756489 J.P.Hosler, S.Ferguson-Miller, and D.A.Mills (2006).
Energy transduction: proton transfer through the respiratory complexes.
  Annu Rev Biochem, 75, 165-187.  
16987808 M.G.Ding, J.P.di Rago, and B.L.Trumpower (2006).
Investigating the Qn site of the cytochrome bc1 complex in Saccharomyces cerevisiae with mutants resistant to ilicicolin H, a novel Qn site inhibitor.
  J Biol Chem, 281, 36036-36043.  
16616870 N.V.Dudkina, J.Heinemeyer, S.Sunderhaus, E.J.Boekema, and H.P.Braun (2006).
Respiratory chain supercomplexes in the plant mitochondrial membrane.
  Trends Plant Sci, 11, 232-240.  
16908520 R.Covian, and B.L.Trumpower (2006).
Regulatory interactions between ubiquinol oxidation and ubiquinone reduction sites in the dimeric cytochrome bc1 complex.
  J Biol Chem, 281, 30925-30932.  
16407191 R.Horsefield, V.Yankovskaya, G.Sexton, W.Whittingham, K.Shiomi, S.Omura, B.Byrne, G.Cecchini, and S.Iwata (2006).
Structural and computational analysis of the quinone-binding site of complex II (succinate-ubiquinone oxidoreductase): a mechanism of electron transfer and proton conduction during ubiquinone reduction.
  J Biol Chem, 281, 7309-7316.
PDB codes: 2acz 2ad0
16504567 T.M.Iverson (2006).
Evolution and unique bioenergetic mechanisms in oxygenic photosynthesis.
  Curr Opin Chem Biol, 10, 91.  
  16946459 T.P.Roosild, S.Castronovo, and S.Choe (2006).
Structure of anti-FLAG M2 Fab domain and its use in the stabilization of engineered membrane proteins.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 62, 835-839.
PDB code: 2g60
16433558 T.Teschner, L.Yatsunyk, V.Schünemann, H.Paulsen, H.Winkler, C.Hu, W.R.Scheidt, F.A.Walker, and A.X.Trautwein (2006).
Models of the membrane-bound cytochromes: mössbauer spectra of crystalline low-spin ferriheme complexes having axial ligand plane dihedral angles ranging from 0 degree to 90 degrees.
  J Am Chem Soc, 128, 1379-1389.  
16756511 W.A.Cramer, H.Zhang, J.Yan, G.Kurisu, and J.L.Smith (2006).
Transmembrane traffic in the cytochrome b6f complex.
  Annu Rev Biochem, 75, 769-790.  
16436386 Y.Matoba, T.Kumagai, A.Yamamoto, H.Yoshitsu, and M.Sugiyama (2006).
Crystallographic evidence that the dinuclear copper center of tyrosinase is flexible during catalysis.
  J Biol Chem, 281, 8981-8990.
PDB codes: 1wx2 1wx3 1wx4 1wx5 1wxc 2ahk 2ahl 2zmx
15817393 A.Osyczka, C.C.Moser, and P.L.Dutton (2005).
Fixing the Q cycle.
  Trends Biochem Sci, 30, 176-182.  
15878858 B.Gurung, L.Yu, D.Xia, and C.A.Yu (2005).
The iron-sulfur cluster of the Rieske iron-sulfur protein functions as a proton-exiting gate in the cytochrome bc(1) complex.
  J Biol Chem, 280, 24895-24902.  
15792955 B.Marbois, P.Gin, K.F.Faull, W.W.Poon, P.T.Lee, J.Strahan, J.N.Shepherd, and C.F.Clarke (2005).
Coq3 and Coq4 define a polypeptide complex in yeast mitochondria for the biosynthesis of coenzyme Q.
  J Biol Chem, 280, 20231-20238.  
15807653 E.Mileykovskaya, M.Zhang, and W.Dowhan (2005).
Cardiolipin in energy transducing membranes.
  Biochemistry (Mosc), 70, 154-158.  
15691836 F.Musiani, A.Dikiy, A.Y.Semenov, and S.Ciurli (2005).
Structure of the intermolecular complex between plastocyanin and cytochrome f from spinach.
  J Biol Chem, 280, 18833-18841.
PDB code: 1ylb
  16511142 G.Uyeda, A.Cámara-Artigas, J.C.Williams, and J.P.Allen (2005).
New tetragonal form of reaction centers from Rhodobacter sphaeroides and the involvement of a manganese ion at a crystal contact point.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 61, 733-736.  
16211522 H.Zhang, and W.A.Cramer (2005).
Problems in obtaining diffraction-quality crystals of hetero-oligomeric integral membrane proteins.
  J Struct Funct Genomics, 6, 219-223.  
16024040 L.S.Huang, D.Cobessi, E.Y.Tung, and E.A.Berry (2005).
Binding of the respiratory chain inhibitor antimycin to the mitochondrial bc1 complex: a new crystal structure reveals an altered intramolecular hydrogen-bonding pattern.
  J Mol Biol, 351, 573-597.
PDB codes: 1pp9 1ppj 2a06
15632120 M.E.Nelson, G.Finazzi, Q.J.Wang, K.A.Middleton-Zarka, J.Whitmarsh, and T.Kallas (2005).
Cytochrome b6 arginine 214 of Synechococcus sp. PCC 7002, a key residue for quinone-reductase site function and turnover of the cytochrome bf complex.
  J Biol Chem, 280, 10395-10402.  
15615728 M.G.Bertero, R.A.Rothery, N.Boroumand, M.Palak, F.Blasco, N.Ginet, J.H.Weiner, and N.C.Strynadka (2005).
Structural and biochemical characterization of a quinol binding site of Escherichia coli nitrate reductase A.
  J Biol Chem, 280, 14836-14843.
PDB codes: 1y4z 1y5i 1y5l 1y5n
15917236 M.W.Mather, E.Darrouzet, M.Valkova-Valchanova, J.W.Cooley, M.T.McIntosh, F.Daldal, and A.B.Vaidya (2005).
Uncovering the molecular mode of action of the antimalarial drug atovaquone using a bacterial system.
  J Biol Chem, 280, 27458-27465.  
15833742 R.Covian, and B.L.Trumpower (2005).
Rapid electron transfer between monomers when the cytochrome bc1 complex dimer is reduced through center N.
  J Biol Chem, 280, 22732-22740.  
16096988 R.W.Woody (2005).
Heme-heme interactions in tetramers and dimers of hemoglobin subunits: DeVoe theory calculations.
  Chirality, 17, 450-455.  
15615714 X.Gong, L.Yu, D.Xia, and C.A.Yu (2005).
Evidence for electron equilibrium between the two hemes bL in the dimeric cytochrome bc1 complex.
  J Biol Chem, 280, 9251-9257.  
16154994 Z.Y.Guo, S.Lin, J.A.Heinen, C.C.Chang, and T.Y.Chang (2005).
The active site His-460 of human acyl-coenzyme A:cholesterol acyltransferase 1 resides in a hitherto undisclosed transmembrane domain.
  J Biol Chem, 280, 37814-37826.  
14961113 A.Osyczka, C.C.Moser, F.Daldal, and P.L.Dutton (2004).
Reversible redox energy coupling in electron transfer chains.
  Nature, 427, 607-612.  
14977419 A.R.Crofts (2004).
The cytochrome bc1 complex: function in the context of structure.
  Annu Rev Physiol, 66, 689-733.  
14610094 A.Stroh, O.Anderka, K.Pfeiffer, T.Yagi, M.Finel, B.Ludwig, and H.Schägger (2004).
Assembly of respiratory complexes I, III, and IV into NADH oxidase supercomplex stabilizes complex I in Paracoccus denitrificans.
  J Biol Chem, 279, 5000-5007.  
15316016 Vitry, Y.Ouyang, G.Finazzi, F.A.Wollman, and T.Kallas (2004).
The chloroplast Rieske iron-sulfur protein. At the crossroad of electron transport and signal transduction.
  J Biol Chem, 279, 44621-44627.  
14670947 E.B.Gutierrez-Cirlos, T.Merbitz-Zahradnik, and B.L.Trumpower (2004).
Inhibition of the yeast cytochrome bc1 complex by ilicicolin H, a novel inhibitor that acts at the Qn site of the bc1 complex.
  J Biol Chem, 279, 8708-8714.  
15317809 F.L.Muller, Y.Liu, and H.Van Remmen (2004).
Complex III releases superoxide to both sides of the inner mitochondrial membrane.
  J Biol Chem, 279, 49064-49073.  
15039445 G.Brasseur, D.Lemesle-Meunier, F.Reinaud, and B.Meunier (2004).
QO site deficiency can be compensated by extragenic mutations in the hinge region of the iron-sulfur protein in the bc1 complex of Saccharomyces cerevisiae.
  J Biol Chem, 279, 24203-24211.  
15003236 J.F.Allen (2004).
Cytochrome b6f: structure for signalling and vectorial metabolism.
  Trends Plant Sci, 9, 130-137.  
14576156 J.J.Kessl, P.Hill, B.B.Lange, S.R.Meshnick, B.Meunier, and B.L.Trumpower (2004).
Molecular basis for atovaquone resistance in Pneumocystis jirovecii modeled in the cytochrome bc(1) complex of Saccharomyces cerevisiae.
  J Biol Chem, 279, 2817-2824.  
15313237 J.L.Smith, H.Zhang, J.Yan, G.Kurisu, and W.A.Cramer (2004).
Cytochrome bc complexes: a common core of structure and function surrounded by diversity in the outlying provinces.
  Curr Opin Struct Biol, 14, 432-439.  
14593095 J.M.Herrmann, and N.Bonnefoy (2004).
Protein export across the inner membrane of mitochondria: the nature of translocated domains determines the dependence on the Oxa1 translocase.
  J Biol Chem, 279, 2507-2512.  
14766756 M.Fabian, D.Jancura, and G.Palmer (2004).
Two sites of interaction of anions with cytochrome a in oxidized bovine cytochrome c oxidase.
  J Biol Chem, 279, 16170-16177.  
15465852 M.Gimpelev, L.R.Forrest, D.Murray, and B.Honig (2004).
Helical packing patterns in membrane and soluble proteins.
  Biophys J, 87, 4075-4086.  
15153117 N.Fisher, A.C.Brown, G.Sexton, A.Cook, J.Windass, and B.Meunier (2004).
Modeling the Qo site of crop pathogens in Saccharomyces cerevisiae cytochrome b.
  Eur J Biochem, 271, 2264-2271.  
14718526 N.Fisher, C.K.Castleden, I.Bourges, G.Brasseur, G.Dujardin, and B.Meunier (2004).
Human disease-related mutations in cytochrome b studied in yeast.
  J Biol Chem, 279, 12951-12958.  
15573135 N.Nelson, and A.Ben-Shem (2004).
The complex architecture of oxygenic photosynthesis.
  Nat Rev Mol Cell Biol, 5, 971-982.  
14761953 R.Covian, E.B.Gutierrez-Cirlos, and B.L.Trumpower (2004).
Anti-cooperative oxidation of ubiquinol by the yeast cytochrome bc1 complex.
  J Biol Chem, 279, 15040-15049.  
15009199 V.Zara, I.Palmisano, L.Conte, and B.L.Trumpower (2004).
Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b.
  Eur J Biochem, 271, 1209-1218.  
12874282 D.R.Kolling, R.I.Samoilova, J.T.Holland, E.A.Berry, S.A.Dikanov, and A.R.Crofts (2003).
Exploration of ligands to the Qi site semiquinone in the bc1 complex using high-resolution EPR.
  J Biol Chem, 278, 39747-39754.  
14526088 G.Kurisu, H.Zhang, J.L.Smith, and W.A.Cramer (2003).
Structure of the cytochrome b6f complex of oxygenic photosynthesis: tuning the cavity.
  Science, 302, 1009-1014.
PDB codes: 1um3 1vf5
12782631 H.Palsdottir, C.G.Lojero, B.L.Trumpower, and C.Hunte (2003).
Structure of the yeast cytochrome bc1 complex with a hydroxyquinone anion Qo site inhibitor bound.
  J Biol Chem, 278, 31303-31311.
PDB code: 1p84
12702760 H.Zhang, G.Kurisu, J.L.Smith, and W.A.Cramer (2003).
A defined protein-detergent-lipid complex for crystallization of integral membrane proteins: The cytochrome b6f complex of oxygenic photosynthesis.
  Proc Natl Acad Sci U S A, 100, 5160-5163.  
12791689 J.J.Kessl, B.B.Lange, T.Merbitz-Zahradnik, K.Zwicker, P.Hill, B.Meunier, H.Pálsdóttir, C.Hunte, S.Meshnick, and B.L.Trumpower (2003).
Molecular basis for atovaquone binding to the cytochrome bc1 complex.
  J Biol Chem, 278, 31312-31318.  
12672829 J.Yan, and W.A.Cramer (2003).
Functional insensitivity of the cytochrome b6f complex to structure changes in the hinge region of the Rieske iron-sulfur protein.
  J Biol Chem, 278, 20925-20933.  
12525495 K.Xiao, G.Engstrom, S.Rajagukguk, C.A.Yu, L.Yu, B.Durham, and F.Millett (2003).
Effect of famoxadone on photoinduced electron transfer between the iron-sulfur center and cytochrome c1 in the cytochrome bc1 complex.
  J Biol Chem, 278, 11419-11426.  
12626741 M.P.Washburn, A.Koller, G.Oshiro, R.R.Ulaszek, D.Plouffe, C.Deciu, E.Winzeler, and J.R.Yates (2003).
Protein pathway and complex clustering of correlated mRNA and protein expression analyses in Saccharomyces cerevisiae.
  Proc Natl Acad Sci U S A, 100, 3107-3112.  
14567700 M.Ritter, O.Anderka, B.Ludwig, W.Mäntele, and P.Hellwig (2003).
Electrochemical and FTIR spectroscopic characterization of the cytochrome bc1 complex from Paracoccus denitrificans: evidence for protonation reactions coupled to quinone binding.
  Biochemistry, 42, 12391-12399.  
  18629088 P.Aloy, and R.B.Russell (2003).
Understanding and Predicting Protein Assemblies With 3D Structures.
  Comp Funct Genomics, 4, 410-415.  
12936966 P.Hill, J.Kessl, N.Fisher, S.Meshnick, B.L.Trumpower, and B.Meunier (2003).
Recapitulation in Saccharomyces cerevisiae of cytochrome b mutations conferring resistance to atovaquone in Pneumocystis jiroveci.
  Antimicrob Agents Chemother, 47, 2725-2731.  
14622010 T.Merbitz-Zahradnik, K.Zwicker, J.H.Nett, T.A.Link, and B.L.Trumpower (2003).
Elimination of the disulfide bridge in the Rieske iron-sulfur protein allows assembly of the [2Fe-2S] cluster into the Rieske protein but damages the ubiquinol oxidation site in the cytochrome bc1 complex.
  Biochemistry, 42, 13637-13645.  
11959503 B.Byrne, and S.Iwata (2002).
Membrane protein complexes.
  Curr Opin Struct Biol, 12, 239-243.  
12163074 C.Hunte, and H.Michel (2002).
Crystallisation of membrane proteins mediated by antibody fragments.
  Curr Opin Struct Biol, 12, 503-508.  
11880631 C.Lange, and C.Hunte (2002).
Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c.
  Proc Natl Acad Sci U S A, 99, 2800-2805.
PDB code: 1kyo
12146165 D.W.Bartlett, J.M.Clough, J.R.Godwin, A.A.Hall, M.Hamer, and B.Parr-Dobrzanski (2002).
The strobilurin fungicides.
  Pest Manag Sci, 58, 649-662.  
12377760 E.B.Gutierrez-Cirlos, T.Merbitz-Zahradnik, and B.L.Trumpower (2002).
Failure to insert the iron-sulfur cluster into the Rieske iron-sulfur protein impairs both center N and center P of the cytochrome bc1 complex.
  J Biol Chem, 277, 50703-50709.  
12045199 G.Engstrom, K.Xiao, C.A.Yu, L.Yu, B.Durham, and F.Millett (2002).
Photoinduced electron transfer between the Rieske iron-sulfur protein and cytochrome c(1) in the Rhodobacter sphaeroides cytochrome bc(1) complex. Effects of pH, temperature, and driving force.
  J Biol Chem, 277, 31072-31078.  
12207018 G.M.Soriano, L.W.Guo, C.De Vitry, T.Kallas, and W.A.Cramer (2002).
Electron transfer from the Rieske iron-sulfur protein (ISP) to cytochrome f in vitro. Is a guided trajectory of the ISP necessary for competent docking?
  J Biol Chem, 277, 41865-41871.  
12364341 M.Zhang, E.Mileykovskaya, and W.Dowhan (2002).
Gluing the respiratory chain together. Cardiolipin is required for supercomplex formation in the inner mitochondrial membrane.
  J Biol Chem, 277, 43553-43556.  
12468233 P.Aloy, and R.B.Russell (2002).
The third dimension for protein interactions and complexes.
  Trends Biochem Sci, 27, 633-638.  
11748214 R.I.Samoilova, D.Kolling, T.Uzawa, T.Iwasaki, A.R.Crofts, and S.A.Dikanov (2002).
The interaction of the Rieske iron-sulfur protein with occupants of the Qo-site of the bc1 complex, probed by electron spin echo envelope modulation.
  J Biol Chem, 277, 4605-4608.  
12384503 Y.Saint-Georges, N.Bonnefoy, J.P.di Rago, S.Chiron, and G.Dujardin (2002).
A pathogenic cytochrome b mutation reveals new interactions between subunits of the mitochondrial bc1 complex.
  J Biol Chem, 277, 49397-49402.  
  11415978 C.G.Tate (2001).
A feast of membrane protein structures in Madrid. Workshop: Pumps, channels and transporters: structure and function.
  EMBO Rep, 2, 476-480.  
11726495 C.Lange, J.H.Nett, B.L.Trumpower, and C.Hunte (2001).
Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structure.
  EMBO J, 20, 6591-6600.
PDB code: 1kb9
11440857 E.Darrouzet, C.C.Moser, P.L.Dutton, and F.Daldal (2001).
Large scale domain movement in cytochrome bc(1): a new device for electron transfer in proteins.
  Trends Biochem Sci, 26, 445-451.  
11266358 G.H.Thomas (2001).
New routes to membrane protein structures. Practical course: current methods in membrane protein research.
  EMBO Rep, 2, 187-191.  
11562368 K.Xiao, A.Chandrasekaran, L.Yu, and C.A.Yu (2001).
Evidence for the intertwined dimer of the cytochrome bc(1) complex in solution.
  J Biol Chem, 276, 46125-46131.  
11722564 R.Covián, and R.Moreno-Sánchez (2001).
Role of protonatable groups of bovine heart bc(1) complex in ubiquinol binding and oxidation.
  Eur J Biochem, 268, 5783-5790.  
11717439 Y.Saint-Georges, P.Hamel, C.Lemaire, and G.Dujardin (2001).
Role of positively charged transmembrane segments in the insertion and assembly of mitochondrial inner-membrane proteins.
  Proc Natl Acad Sci U S A, 98, 13814-13819.  
11054826 A.Blasco, and P.Sanz (2000).
Current awareness on yeast.
  Yeast, 16, 1449-1456.  
11004461 E.A.Berry, Z.Zhang, H.D.Bellamy, and L.Huang (2000).
Crystallographic location of two Zn(2+)-binding sites in the avian cytochrome bc(1) complex.
  Biochim Biophys Acta, 1459, 440-448.
PDB code: 3h1k
10971589 J.H.Nett, C.Hunte, and B.L.Trumpower (2000).
Changes to the length of the flexible linker region of the Rieske protein impair the interaction of ubiquinol with the cytochrome bc1 complex.
  Eur J Biochem, 267, 5777-5782.  
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