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PDBsum entry 1eon

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protein dna_rna ligands metals Protein-protein interface(s) links
Hydrolase/DNA PDB id
1eon
Jmol
Contents
Protein chains
236 a.a.
DNA/RNA
Ligands
ACY ×2
Metals
_CL ×4
Waters ×357
PDB id:
1eon
Name: Hydrolase/DNA
Title: Ecorv bound to 3'-s-phosphorothiolate DNA and ca2+
Structure: DNA (5'-d( Ap Ap Ap Gp Ap (Tsp)p Ap Tp Cp Tp T)- 3'). Chain: c. Engineered: yes. DNA (5'-d( Cp Ap Ap Gp Ap (Tsp)p Ap Tp Cp Tp T)- 3'). Chain: d. Engineered: yes. Type ii restriction enzyme ecorv.
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Tetramer (from PQS)
Resolution:
1.60Å     R-factor:   0.229     R-free:   0.281
Authors: N.C.Horton,B.A.Connolly,J.J.Perona
Key ref: N.C.Horton et al. (2000). Inhibition of ecorv endonuclease by deoxyribo-3'-S-Phosphorothiolates: a high-Resolution x-Ray crystallographic study. J.Am.Chem.Soc., 122, 3314-3324.
Date:
23-Mar-00     Release date:   04-Apr-00    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P04390  (T2E5_ECOLX) -  Type-2 restriction enzyme EcoRV
Seq:
Struc:
245 a.a.
236 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.1.21.4  - Type Ii site-specific deoxyribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
      Cofactor: Magnesium
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     nucleic acid phosphodiester bond hydrolysis   3 terms 
  Biochemical function     hydrolase activity     6 terms