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Translation PDB id
1ej1
Jmol
Contents
Protein chains
178 a.a. *
190 a.a. *
Ligands
M7G ×2
Waters ×130
* Residue conservation analysis
PDB id:
1ej1
Name: Translation
Title: Cocrystal structure of the messenger RNA 5' cap-binding protein (eif4e) bound to 7-methyl-gdp
Structure: Eukaryotic translation initiation factor 4e. Chain: a, b. Fragment: residues 28-217. Engineered: yes
Source: Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Dimer (from PQS)
Resolution:
2.20Å     R-factor:   0.209     R-free:   0.235
Authors: J.Marcotrigiano,A.-C.Gingras,N.Sonenberg,S.K.Burley
Key ref:
J.Marcotrigiano et al. (1997). Cocrystal structure of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP. Cell, 89, 951-961. PubMed id: 9200613 DOI: 10.1016/S0092-8674(00)80280-9
Date:
29-Feb-00     Release date:   15-Mar-00    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P63073  (IF4E_MOUSE) -  Eukaryotic translation initiation factor 4E
Seq:
Struc: 		217 a.a.
178 a.a.
Protein chain
Pfam   ArchSchema ?
P63073  (IF4E_MOUSE) -  Eukaryotic translation initiation factor 4E
Seq:
Struc: 		217 a.a.
190 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   1 term 
  Biological process     translational initiation   1 term 
  Biochemical function     RNA binding     2 terms  

 

 
DOI no: 10.1016/S0092-8674(00)80280-9 Cell 89:951-961 (1997)
PubMed id: 9200613  
 
 
Cocrystal structure of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP.
J.Marcotrigiano, A.C.Gingras, N.Sonenberg, S.K.Burley.
 
  ABSTRACT  
 
The X-ray structure of the eukaryotic translation initiation factor 4E (eIF4E), bound to 7-methyl-GDP, has been determined at 2.2 A resolution. eIF4E recognizes 5' 7-methyl-G(5')ppp(5')N mRNA caps during the rate-limiting initiation step of translation. The protein resembles a cupped hand and consists of a curved, 8-stranded antiparallel beta sheet, backed by three long alpha helices. 7-methyl-GDP binds in a narrow cap-binding slot on the molecule's concave surface, where 7-methyl-guanine recognition is mediated by base sandwiching between two conserved tryptophans, plus formation of three hydrogen bonds and a van der Waals contact between its N7-methyl group and a third conserved tryptophan. The convex dorsal surface of the molecule displays a phylogenetically conserved hydrophobic/acidic portion, which may interact with other translation initiation factors and regulatory proteins.
 
  Selected figure(s)  
 
Figure 1.
Figure 1. eIF4E Sequence AlignmentsSequence alignments of eIF4E from mouse ([5]), human ( [51]), rat ( [41]), rabbit ( [50]), Xenopus laevis ( [63]), Drosophila melanogaster ( [21]), Saccharomyces cerevisiae ( [3]), Schizosaccharomyces pombe ( [48]), and wheat germ ( [39]), with their respective sequence numbers. The secondary structural elements were assigned from the X-ray structure. Boldface W indicates absolutely conserved tryptophan. Functional classifications: boldface s, π stacking tryptophan; boldface g, hydrogen bonded to guanine; boldface r, stabilizing Arg-157; boldface p, interaction with phosphate groups of 7-methyl-GDP; boldface m, van der Waals interaction with methyl group of 7-methyl-GDP; asterisk, site of phosphorylation in vivo at Ser-209. Environment classification: =, solvent-accessible side chain. 		Figure 3.
Figure 3. Structure of the Murine eIF4E-7-methyl-GDP Complex(A) RIBBONS ([9]) stereodrawing showing the concave cap-binding surface of eIF4E(28–217). 7-methyl-GDP, included as an atomic stick figure, is located in the cap-binding slot. α helices are labeled H1-H4 and β strands are labeled 1–8, with the N and C termini labeled with N and C, respectively. The 5′-untranslated region of the mRNA would presumably project down and left to the entrance of the cap-binding slot, overlying helix H3 and strands S5, S6, and S4.(B) Stereo drawing viewed along the face of the β sheet, showing eIF4E in profile and the location of the α helices on the molecule's convex dorsal surface.(C) Stereo drawing viewed along the face of the β sheet, showing 7-methyl-GDP and the entrance to the cap-binding slot.(D) Stereo drawing viewed perpendicular to the β strands, showing the convex dorsal surface of eIF4E with its three long α helices.
 
  The above figures are reprinted by permission from Cell Press: Cell (1997, 89, 951-961) copyright 1997.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21283665 J.A.Ashby, C.E.Stevenson, G.E.Jarvis, D.M.Lawson, and A.J.Maule (2011).
Structure-Based Mutational Analysis of eIF4E in Relation to sbm1 Resistance to Pea Seed-Borne Mosaic Virus in Pea.
  PLoS One, 6, e15873.
PDB code: 2wmc
20535623 K.Ruszczyńska-Bartnik, M.Maciejczyk, and R.Stolarski (2011).
Dynamical insight into Caenorhabditis elegans eIF4E recognition specificity for mono-and trimethylated structures of mRNA 5' cap.
  J Mol Model, 17, 727-737.  
20539312 A.Boland, F.Tritschler, S.Heimstädt, E.Izaurralde, and O.Weichenrieder (2010).
Crystal structure and ligand binding of the MID domain of a eukaryotic Argonaute protein.
  EMBO Rep, 11, 522-527.
PDB code: 2xdy
20648000 C.Goetz, R.G.Everson, L.C.Zhang, and M.Gromeier (2010).
MAPK signal-integrating kinase controls cap-independent translation and cell type-specific cytotoxicity of an oncolytic poliovirus.
  Mol Ther, 18, 1937-1946.  
20593023 F.Piron, M.Nicolaï, S.Minoïa, E.Piednoir, A.Moretti, A.Salgues, D.Zamir, C.Caranta, and A.Bendahmane (2010).
An induced mutation in tomato eIF4E leads to immunity to two potyviruses.
  PLoS One, 5, e11313.  
20964625 J.Y.Yin, Z.Dong, Z.Q.Liu, and J.T.Zhang (2010).
Translational control gone awry: a new mechanism of tumorigenesis and novel targets of cancer treatments.
  Biosci Rep, 31, 1.  
20383144 K.Das, J.M.Aramini, L.C.Ma, R.M.Krug, and E.Arnold (2010).
Structures of influenza A proteins and insights into antiviral drug targets.
  Nat Struct Mol Biol, 17, 530-538.  
20854261 K.Wollenhaupt, K.Reinke, K.P.Brüssow, D.Albrecht, U.Tiemann, H.M.Seyfert, and W.Tomek (2010).
Natural occurrence and physiological role of a truncated eIF4E in the porcine endometrium during implantation.
  Biochem J, 432, 353-363.  
20062058 S.Djuranovic, M.K.Zinchenko, J.K.Hur, A.Nahvi, J.L.Brunelle, E.J.Rogers, and R.Green (2010).
Allosteric regulation of Argonaute proteins by miRNAs.
  Nat Struct Mol Biol, 17, 144-150.  
19114555 A.Yanagiya, Y.V.Svitkin, S.Shibata, S.Mikami, H.Imataka, and N.Sonenberg (2009).
Requirement of RNA binding of mammalian eukaryotic translation initiation factor 4GI (eIF4GI) for efficient interaction of eIF4E with the mRNA cap.
  Mol Cell Biol, 29, 1661-1669.  
19782035 A.Zdanowicz, R.Thermann, J.Kowalska, J.Jemielity, K.Duncan, T.Preiss, E.Darzynkiewicz, and M.W.Hentze (2009).
Drosophila miR2 primarily targets the m7GpppN cap structure for translational repression.
  Mol Cell, 35, 881-888.  
  19351181 B.Ghosh, A.O.Benyumov, P.Ghosh, Y.Jia, S.Avdulov, P.S.Dahlberg, M.Peterson, K.Smith, V.A.Polunovsky, P.B.Bitterman, and C.R.Wagner (2009).
Nontoxic chemical interdiction of the epithelial-to-mesenchymal transition by targeting cap-dependent translation.
  ACS Chem Biol, 4, 367-377.  
19729311 D.R.Schoenberg, and L.E.Maquat (2009).
Re-capping the message.
  Trends Biochem Sci, 34, 435-442.  
19122207 H.Okade, Y.Fujita, S.Miyamoto, K.Tomoo, S.Muto, H.Miyoshi, T.Natsuaki, R.E.Rhoads, and T.Ishida (2009).
Turnip mosaic virus genome-linked protein VPg binds C-terminal region of cap-bound initiation factor 4E orthologue without exhibiting host cellular specificity.
  J Biochem, 145, 299-307.  
19884259 H.Sato, and L.E.Maquat (2009).
Remodeling of the pioneer translation initiation complex involves translation and the karyopherin importin beta.
  Genes Dev, 23, 2537-2550.  
19114552 I.Topisirovic, N.Siddiqui, S.Orolicki, L.A.Skrabanek, M.Tremblay, T.Hoang, and K.L.Borden (2009).
Stability of eukaryotic translation initiation factor 4E mRNA is regulated by HuR, and this activity is dysregulated in cancer.
  Mol Cell Biol, 29, 1152-1162.  
19820912 K.S.Ling, K.R.Harris, J.D.Meyer, A.Levi, N.Guner, T.C.Wehner, A.Bendahmane, and M.J.Havey (2009).
Non-synonymous single nucleotide polymorphisms in the watermelon eIF4E gene are closely associated with resistance to Zucchini yellow mosaic virus.
  Theor Appl Genet, 120, 191-200.  
19858189 M.A.Khan, H.Yumak, and D.J.Goss (2009).
Kinetic mechanism for the binding of eIF4F and tobacco Etch virus internal ribosome entry site rna: effects of eIF4B and poly(A)-binding protein.
  J Biol Chem, 284, 35461-35470.  
19217398 M.Wu, P.Nilsson, N.Henriksson, A.Niedzwiecka, M.K.Lim, Z.Cheng, K.Kokkoris, A.Virtanen, and H.Song (2009).
Structural basis of m(7)GpppG binding to poly(A)-specific ribonuclease.
  Structure, 17, 276-286.  
19414591 N.M.Kaye, K.J.Emmett, W.C.Merrick, and E.Jankowsky (2009).
Intrinsic RNA binding by the eukaryotic initiation factor 4F depends on a minimal RNA length but not on the m7G cap.
  J Biol Chem, 284, 17742-17750.  
19237539 R.E.Rhoads (2009).
eIF4E: New Family Members, New Binding Partners, New Roles.
  J Biol Chem, 284, 16711-16715.  
  19641745 R.Tuteja (2009).
Identification and bioinformatics characterization of translation initiation complex eIF4F components and poly(A)-binding protein from Plasmodium falciparum.
  Commun Integr Biol, 2, 245-260.  
19750007 S.S.Bradrick, and M.Gromeier (2009).
Identification of gemin5 as a novel 7-methylguanosine cap-binding protein.
  PLoS One, 4, e7030.  
19952439 T.Ishida (2009).
Structural studies of specific intermolecular interactions and self-aggregation of biomolecules and their application to drug design.
  Chem Pharm Bull (Tokyo), 57, 1309-1334.  
19710013 W.Liu, R.Zhao, C.McFarland, J.Kieft, A.Niedzwiecka, M.Jankowska-Anyszka, J.Stepinski, E.Darzynkiewicz, D.N.Jones, and R.E.Davis (2009).
Structural insights into parasite eIF4E binding specificity for m7G and m2,2,7G mRNA caps.
  J Biol Chem, 284, 31336-31349.
PDB codes: 3hxg 3hxi
19272168 X.Gao, and D.Xing (2009).
Molecular mechanisms of cell proliferation induced by low power laser irradiation.
  J Biomed Sci, 16, 4.  
19276085 Z.Wang, K.Treder, and W.A.Miller (2009).
Structure of a Viral Cap-independent Translation Element That Functions via High Affinity Binding to the eIF4E Subunit of eIF4F.
  J Biol Chem, 284, 14189-14202.  
18345015 A.Eulalio, E.Huntzinger, and E.Izaurralde (2008).
GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay.
  Nat Struct Mol Biol, 15, 346-353.  
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PDB code: 2vqz
18928538 I.Y.Tcherepanova, M.D.Adams, X.Feng, A.Hinohara, J.Horvatinovich, D.Calderhead, D.Healey, and C.A.Nicolette (2008).
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  BMC Mol Biol, 9, 90.  
18953590 J.R.Cavatorta, A.E.Savage, I.Yeam, S.M.Gray, and M.M.Jahn (2008).
Positive Darwinian selection at single amino acid sites conferring plant virus resistance.
  J Mol Evol, 67, 551-559.  
18025255 K.Treder, E.L.Kneller, E.M.Allen, Z.Wang, K.S.Browning, and W.A.Miller (2008).
The 3' cap-independent translation element of Barley yellow dwarf virus binds eIF4F via the eIF4G subunit to initiate translation.
  RNA, 14, 134-147.  
18971945 M.A.Mir, and A.T.Panganiban (2008).
A protein that replaces the entire cellular eIF4F complex.
  EMBO J, 27, 3129-3139.  
18280238 M.V.Deshmukh, B.N.Jones, D.U.Quang-Dang, J.Flinders, S.N.Floor, C.Kim, J.Jemielity, M.Kalek, E.Darzynkiewicz, and J.D.Gross (2008).
mRNA decapping is promoted by an RNA-binding channel in Dcp2.
  Mol Cell, 29, 324-336.
PDB code: 2jvb
18479935 P.Ghosh, J.Cheng, T.F.Chou, Y.Jia, S.Avdulov, P.B.Bitterman, V.A.Polunovsky, and C.R.Wagner (2008).
Expression, purification and characterization of recombinant mouse translation initiation factor eIF4E as a dihydrofolate reductase (DHFR) fusion protein.
  Protein Expr Purif, 60, 132-139.  
18186485 R.Worch, and R.Stolarski (2008).
Stacking efficiency and flexibility analysis of aromatic amino acids in cap-binding proteins.
  Proteins, 71, 2026-2037.  
18480444 S.German-Retana, J.Walter, B.Doublet, G.Roudet-Tavert, V.Nicaise, C.Lecampion, M.C.Houvenaghel, C.Robaglia, T.Michon, and O.Le Gall (2008).
Mutational analysis of plant cap-binding protein eIF4E reveals key amino acids involved in biochemical functions and potyvirus infection.
  J Virol, 82, 7601-7612.  
18614538 S.V.Slepenkov, N.L.Korneeva, and R.E.Rhoads (2008).
Kinetic mechanism for assembly of the m7GpppG.eIF4E.eIF4G complex.
  J Biol Chem, 283, 25227-25237.  
18197166 W.Filipowicz, S.N.Bhattacharyya, and N.Sonenberg (2008).
Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?
  Nat Rev Genet, 9, 102-114.  
17268778 B.Kierdaszuk, and J.WÅ‚odarczyk (2007).
Interpretation of fluorescence decay kinetics in 3-methylbenzimidazolyl(5'-5')guanosine dinucleotides: exponential dependence on the number of phosphates in the polyphosphate bridge.
  Eur Biophys J, 36, 253-259.  
17584936 C.Nieto, F.Piron, M.Dalmais, C.F.Marco, E.Moriones, M.L.Gómez-Guillamón, V.Truniger, P.Gómez, J.Garcia-Mas, M.A.Aranda, and A.Bendahmane (2007).
EcoTILLING for the identification of allelic variants of melon eIF4E, a factor that controls virus susceptibility.
  BMC Plant Biol, 7, 34.  
18072984 C.S.Fraser, and J.A.Doudna (2007).
Quantitative studies of ribosome conformational dynamics.
  Q Rev Biophys, 40, 163-189.  
17289916 F.Okumura, W.Zou, and D.E.Zhang (2007).
ISG15 modification of the eIF4E cognate 4EHP enhances cap structure-binding activity of 4EHP.
  Genes Dev, 21, 255-260.  
18055695 H.G.Wendel, R.L.Silva, A.Malina, J.R.Mills, H.Zhu, T.Ueda, R.Watanabe-Fukunaga, R.Fukunaga, J.Teruya-Feldstein, J.Pelletier, and S.W.Lowe (2007).
Dissecting eIF4E action in tumorigenesis.
  Genes Dev, 21, 3232-3237.  
17490476 H.K.Saini, and D.Fischer (2007).
Structural and functional insights into Mimivirus ORFans.
  BMC Genomics, 8, 115.  
17369309 J.Zuberek, D.Kubacka, A.Jablonowska, J.Jemielity, J.Stepinski, N.Sonenberg, and E.Darzynkiewicz (2007).
Weak binding affinity of human 4EHP for mRNA cap analogs.
  RNA, 13, 691-697.  
17524464 M.Kiriakidou, G.S.Tan, S.Lamprinaki, M.De Planell-Saguer, P.T.Nelson, and Z.Mourelatos (2007).
An mRNA m7G cap binding-like motif within human Ago2 represses translation.
  Cell, 129, 1141-1151.  
17942399 N.Minshall, M.H.Reiter, D.Weil, and N.Standart (2007).
CPEB interacts with an ovary-specific eIF4E and 4E-T in early Xenopus oocytes.
  J Biol Chem, 282, 37389-37401.  
17785461 P.Nilsson, N.Henriksson, A.Niedzwiecka, N.A.Balatsos, K.Kokkoris, J.Eriksson, and A.Virtanen (2007).
A multifunctional RNA recognition motif in poly(A)-specific ribonuclease with cap and poly(A) binding properties.
  J Biol Chem, 282, 32902-32911.  
17091471 T.M.Goldson, G.Vielhauer, E.Staub, S.Miller, H.Shim, and C.H.Hagedorn (2007).
Eukaryotic initiation factor 4E variants alter the morphology, proliferation, and colony-formation properties of MDA-MB-435 cancer cells.
  Mol Carcinog, 46, 71-84.  
16886072 A.Schmidt, A.Lindner, M.Nieger, M.d.e.l. .C.Ruiz-Delgado, and F.J.Ramirez (2006).
Syntheses, pi-stacking interactions and base-pairings of uracil pyridinium salts and uracilyl betaines with nucleobases.
  Org Biomol Chem, 4, 3056-3066.  
16518651 B.Kierdaszuk, and J.WÅ‚odarczyk (2006).
Interpretation of intramolecular stacking effect on the fluorescence intensity decay of 3-methylbenzimidazolyl(5'-5')guanosine dinucleotides using a model of lifetime distribution.
  Eur Biophys J, 35, 424-430.  
17026540 C.Nieto, M.Morales, G.Orjeda, C.Clepet, A.Monfort, B.Sturbois, P.Puigdomènech, M.Pitrat, M.Caboche, C.Dogimont, J.Garcia-Mas, M.A.Aranda, and A.Bendahmane (2006).
An eIF4E allele confers resistance to an uncapped and non-polyadenylated RNA virus in melon.
  Plant J, 48, 452-462.  
16257956 E.Grudzien, M.Kalek, J.Jemielity, E.Darzynkiewicz, and R.E.Rhoads (2006).
Differential inhibition of mRNA degradation pathways by novel cap analogs.
  J Biol Chem, 281, 1857-1867.  
17036047 L.Volpon, M.J.Osborne, I.Topisirovic, N.Siddiqui, and K.L.Borden (2006).
Cap-free structure of eIF4E suggests a basis for conformational regulation by its ligands.
  EMBO J, 25, 5138-5149.
PDB code: 2gpq
17028101 M.P.Hall, and C.K.Ho (2006).
Functional characterization of a 48 kDa Trypanosoma brucei cap 2 RNA methyltransferase.
  Nucleic Acids Res, 34, 5594-5602.  
17055983 P.F.Cho, C.Gamberi, Y.A.Cho-Park, I.B.Cho-Park, P.Lasko, and N.Sonenberg (2006).
Cap-dependent translational inhibition establishes two opposing morphogen gradients in Drosophila embryos.
  Curr Biol, 16, 2035-2041.  
16715044 S.P.Ryder (2006).
Oskar gains weight.
  Nat Struct Mol Biol, 13, 297-299.  
16540463 S.V.Slepenkov, E.Darzynkiewicz, and R.E.Rhoads (2006).
Stopped-flow kinetic analysis of eIF4E and phosphorylated eIF4E binding to cap analogs and capped oligoribonucleotides: evidence for a one-step binding mechanism.
  J Biol Chem, 281, 14927-14938.  
16519694 T.Michon, Y.Estevez, J.Walter, S.German-Retana, and O.Le Gall (2006).
The potyviral virus genome-linked protein VPg forms a ternary complex with the eukaryotic initiation factors eIF4E and eIF4G and reduces eIF4E affinity for a mRNA cap analogue.
  FEBS J, 273, 1312-1322.  
16720573 T.Murata, and K.Shimotohno (2006).
Ubiquitination and proteasome-dependent degradation of human eukaryotic translation initiation factor 4E.
  J Biol Chem, 281, 20788-20800.  
16472736 V.Shen, and M.Kiledjian (2006).
Decapper comes into focus.
  Structure, 14, 171-172.  
17041189 Y.Yoffe, J.Zuberek, A.Lerer, M.Lewdorowicz, J.Stepinski, M.Altmann, E.Darzynkiewicz, and M.Shapira (2006).
Binding specificities and potential roles of isoforms of eukaryotic initiation factor 4E in Leishmania.
  Eukaryot Cell, 5, 1969-1979.  
15920472 A.Strasser, A.Dickmanns, R.Lührmann, and R.Ficner (2005).
Structural basis for m3G-cap-mediated nuclear import of spliceosomal UsnRNPs by snurportin1.
  EMBO J, 24, 2235-2243.
PDB code: 1xk5
15842624 B.C.Kang, I.Yeam, J.D.Frantz, J.F.Murphy, and M.M.Jahn (2005).
The pvr1 locus in Capsicum encodes a translation initiation factor eIF4E that interacts with Tobacco etch virus VPg.
  Plant J, 42, 392-405.  
15837800 B.Culjkovic, I.Topisirovic, L.Skrabanek, M.Ruiz-Gutierrez, and K.L.Borden (2005).
eIF4E promotes nuclear export of cyclin D1 mRNAs via an element in the 3'UTR.
  J Cell Biol, 169, 245-256.  
16191198 B.Joshi, K.Lee, D.L.Maeder, and R.Jagus (2005).
Phylogenetic analysis of eIF4E-family members.
  BMC Evol Biol, 5, 48.  
16288289 D.Ruggero, and N.Sonenberg (2005).
The Akt of translational control.
  Oncogene, 24, 7426-7434.  
  16511166 E.Bitto, C.A.Bingman, H.Robinson, S.T.Allard, G.E.Wesenberg, and G.N.Phillips (2005).
The structure at 2.5 A resolution of human basophilic leukemia-expressed protein BLES03.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 61, 812-817.
PDB code: 1ztp
15690031 J.D.Richter, and N.Sonenberg (2005).
Regulation of cap-dependent translation by eIF4E inhibitory proteins.
  Nature, 433, 477-480.  
15988567 J.P.Combe, M.E.Petracek, G.van Eldik, F.Meulewaeter, and D.Twell (2005).
Translation initiation factors eIF4E and eIFiso4E are required for polysome formation and regulate plant growth in tobacco.
  Plant Mol Biol, 57, 749-760.  
15611299 K.Mochizuki, A.Oguro, T.Ohtsu, N.Sonenberg, and Y.Nakamura (2005).
High affinity RNA for mammalian initiation factor 4E interferes with mRNA-cap binding and inhibits translation.
  RNA, 11, 77-89.  
15879529 L.Li, and C.C.Wang (2005).
Identification in the ancient protist Giardia lamblia of two eukaryotic translation initiation factor 4E homologues with distinctive functions.
  Eukaryot Cell, 4, 948-959.  
15941403 N.Stein, D.Perovic, J.Kumlehn, B.Pellio, S.Stracke, S.Streng, F.Ordon, and A.Graner (2005).
The eukaryotic translation initiation factor 4E confers multiallelic recessive Bymovirus resistance in Hordeum vulgare (L.).
  Plant J, 42, 912-922.  
15882623 P.F.Cho, F.Poulin, Y.A.Cho-Park, I.B.Cho-Park, J.D.Chicoine, P.Lasko, and N.Sonenberg (2005).
A new paradigm for translational control: inhibition via 5'-3' mRNA tethering by Bicoid and the eIF4E cognate 4EHP.
  Cell, 121, 411-423.  
16222335 R.Majumdar, and U.Maitra (2005).
Regulation of GTP hydrolysis prior to ribosomal AUG selection during eukaryotic translation initiation.
  EMBO J, 24, 3737-3746.  
15900314 S.J.Morley, M.J.Coldwell, and M.J.Clemens (2005).
Initiation factor modifications in the preapoptotic phase.
  Cell Death Differ, 12, 571-584.  
15971038 S.Ruffel, J.L.Gallois, M.L.Lesage, and C.Caranta (2005).
The recessive potyvirus resistance gene pot-1 is the tomato orthologue of the pepper pvr2-eIF4E gene.
  Mol Genet Genomics, 274, 346-353.  
15601834 T.D.Dinkova, B.D.Keiper, N.L.Korneeva, E.J.Aamodt, and R.E.Rhoads (2005).
Translation of a small subset of Caenorhabditis elegans mRNAs is dependent on a specific eukaryotic translation initiation factor 4E isoform.
  Mol Cell Biol, 25, 100-113.  
16211509 X.Gao, K.Bain, J.B.Bonanno, M.Buchanan, D.Henderson, D.Lorimer, C.Marsh, J.A.Reynes, J.M.Sauder, K.Schwinn, C.Thai, and S.K.Burley (2005).
High-throughput limited proteolysis/mass spectrometry for protein domain elucidation.
  J Struct Funct Genomics, 6, 129-134.  
16043507 Y.Yan, Y.Svitkin, J.M.Lee, M.Bisaillon, and J.Pelletier (2005).
Ribavirin is not a functional mimic of the 7-methyl guanosine mRNA cap.
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Snurportin1, an m3G-cap-specific nuclear import receptor with a novel domain structure.
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9792652 J.R.Dyer, A.M.Pepio, S.K.Yanow, and W.S.Sossin (1998).
Phosphorylation of eIF4E at a conserved serine in Aplysia.
  J Biol Chem, 273, 29469-29474.  
9553087 K.A.Ruud, C.Kuhlow, D.J.Goss, and K.S.Browning (1998).
Identification and characterization of a novel cap-binding protein from Arabidopsis thaliana.
  J Biol Chem, 273, 10325-10330.  
9468520 M.Gao, W.Rychlik, and R.E.Rhoads (1998).
Cloning and characterization of human eIF4E genes.
  J Biol Chem, 273, 4622-4628.  
9553113 M.Jankowska-Anyszka, B.J.Lamphear, E.J.Aamodt, T.Harrington, E.Darzynkiewicz, R.Stolarski, and R.E.Rhoads (1998).
Multiple isoforms of eukaryotic protein synthesis initiation factor 4E in Caenorhabditis elegans can distinguish between mono- and trimethylated mRNA cap structures.
  J Biol Chem, 273, 10538-10542.  
9875850 M.P.Horvath, V.L.Schweiker, J.M.Bevilacqua, J.A.Ruggles, and S.C.Schultz (1998).
Crystal structure of the Oxytricha nova telomere end binding protein complexed with single strand DNA.
  Cell, 95, 963-974.
PDB code: 1otc
9707439 M.Ptushkina, T.von der Haar, S.Vasilescu, R.Frank, R.Birkenhäger, and J.E.McCarthy (1998).
Cooperative modulation by eIF4G of eIF4E-binding to the mRNA 5' cap in yeast involves a site partially shared by p20.
  EMBO J, 17, 4798-4808.  
9561852 N.Sonenberg, and A.C.Gingras (1998).
The mRNA 5' cap-binding protein eIF4E and control of cell growth.
  Curr Opin Cell Biol, 10, 268-275.  
9501904 N.Sonenberg, S.K.Burley, and A.C.Gingras (1998).
RNA chiropractics.
  Nat Struct Biol, 5, 172-174.  
9482745 T.E.LaGrandeur, and R.Parker (1998).
Isolation and characterization of Dcp1p, the yeast mRNA decapping enzyme.
  EMBO J, 17, 1487-1496.  
9334736 A.Liljas, and S.al-Karadaghi (1997).
Structural aspects of protein synthesis.
  Nat Struct Biol, 4, 767-771.  
  9261078 G.Varani (1997).
A cap for all occasions.
  Structure, 5, 855-858.  
9302999 H.Matsuo, H.Li, A.M.McGuire, C.M.Fletcher, A.C.Gingras, N.Sonenberg, and G.Wagner (1997).
Structure of translation factor eIF4E bound to m7GDP and interaction with 4E-binding protein.
  Nat Struct Biol, 4, 717-724.
PDB code: 1ap8
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.