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PDBsum entry 1ef1

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protein ligands Protein-protein interface(s) links
Membrane protein PDB id
1ef1
Jmol
Contents
Protein chains
294 a.a. *
90 a.a. *
Ligands
SO4 ×2
Waters ×412
* Residue conservation analysis
PDB id:
1ef1
Name: Membrane protein
Title: Crystal structure of the moesin ferm domain/tail domain comp
Structure: Moesin. Chain: a, b. Fragment: n-terminal ferm domain. Engineered: yes. Moesin. Chain: c, d. Fragment: c-terminal tail domain. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
Biol. unit: Tetramer (from PQS)
Resolution:
1.90Å     R-factor:   0.227     R-free:   0.287
Authors: M.A.Pearson,D.Reczek,A.Bretscher,P.A.Karplus
Key ref:
M.A.Pearson et al. (2000). Structure of the ERM protein moesin reveals the FERM domain fold masked by an extended actin binding tail domain. Cell, 101, 259-270. PubMed id: 10847681 DOI: 10.1016/S0092-8674(00)80836-3
Date:
04-Feb-00     Release date:   10-May-00    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P26038  (MOES_HUMAN) -  Moesin
Seq:
Struc:
 
Seq:
Struc:
577 a.a.
294 a.a.
Protein chains
Pfam   ArchSchema ?
P26038  (MOES_HUMAN) -  Moesin
Seq:
Struc:
 
Seq:
Struc:
577 a.a.
90 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   3 terms 
  Biochemical function     cytoskeletal protein binding     2 terms  

 

 
DOI no: 10.1016/S0092-8674(00)80836-3 Cell 101:259-270 (2000)
PubMed id: 10847681  
 
 
Structure of the ERM protein moesin reveals the FERM domain fold masked by an extended actin binding tail domain.
M.A.Pearson, D.Reczek, A.Bretscher, P.A.Karplus.
 
  ABSTRACT  
 
The ezrin-radixin-moesin (ERM) protein family link actin filaments of cell surface structures to the plasma membrane, using a C-terminal F-actin binding segment and an N-terminal FERM domain, a common membrane binding module. ERM proteins are regulated by an intramolecular association of the FERM and C-terminal tail domains that masks their binding sites. The crystal structure of a dormant moesin FERM/tail complex reveals that the FERM domain has three compact lobes including an integrated PTB/PH/ EVH1 fold, with the C-terminal segment bound as an extended peptide masking a large surface of the FERM domain. This extended binding mode suggests a novel mechanism for how different signals could produce varying levels of activation. Sequence conservation suggests a similar regulation of the tumor suppressor merlin.
 
  Selected figure(s)  
 
Figure 6.
Figure 6. Stereodiagram of the Region Surrounding Thr558Thr558 is in van der Waals contact with FERM residues Thr235 and Pro236, and is accessible to solvent from the right between the side chains of Arg553, Lys557, and Gln561. The electron density (from an NCS averaged map calculated with coefficients 2F[O] − 2F[C], αC) is contoured at 2.2 × ρ[rms]. A phosphoryl group modeled onto Thr558 collides with surrounding atoms from Arg553 and Gln561.
Figure 7.
Figure 7. Potential FERM Domain Interaction SitesThe positions of five binding sites on proteins that are structurally similar to the three FERM lobes are indicated: (1) by analogy with the protein interaction site seen for elongin B ([62]) and the Ras binding domain of Raf kinase ( [47]); (2) by analogy with the lipid binding site of the acyl CoA binding protein ( [36]), but this site is blocked by the loop connecting the F2 helices C and D; (3) by analogy with the proline-containing peptide binding site on EVH1 domains ( [53]; Federov et al., 1999); (4) by analogy with the canonical peptide binding site of PTB domains ( [74]); and (5) by analogy with the inositol phosphate binding sites on PH domains ( [13 and 31]).
 
  The above figures are reprinted by permission from Cell Press: Cell (2000, 101, 259-270) copyright 2000.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21366541 C.Willenborg, and R.Prekeris (2011).
Apical protein transport and lumen morphogenesis in polarized epithelial cells.
  Biosci Rep, 31, 245-256.  
21373988 K.L.Jones, R.P.Smyth, C.F.Pereira, P.U.Cameron, S.R.Lewin, A.Jaworowski, and J.Mak (2011).
Early Events of HIV-1 Infection: Can Signaling be the Next Therapeutic Target?
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21463584 S.Bosk, J.A.Braunger, V.Gerke, and C.Steinem (2011).
Activation of F-actin binding capacity of ezrin: synergism of PIP₂ interaction and phosphorylation.
  Biophys J, 100, 1708-1717.  
21321230 Z.Wei, J.Yan, Q.Lu, L.Pan, and M.Zhang (2011).
Cargo recognition mechanism of myosin X revealed by the structure of its tail MyTH4-FERM tandem in complex with the DCC P3 domain.
  Proc Natl Acad Sci U S A, 108, 3572-3577.
PDB code: 3pzd
20001213 C.A.Lipinski, and J.C.Loftus (2010).
Targeting Pyk2 for therapeutic intervention.
  Expert Opin Ther Targets, 14, 95.  
20156804 F.C.Morales, J.R.Molina, Y.Hayashi, and M.M.Georgescu (2010).
Overexpression of ezrin inactivates NF2 tumor suppressor in glioblastoma.
  Neuro Oncol, 12, 528-539.  
20491622 I.Stamenkovic, and Q.Yu (2010).
Merlin, a "magic" linker between extracellular cues and intracellular signaling pathways that regulate cell motility, proliferation, and survival.
  Curr Protein Pept Sci, 11, 471-484.  
20546116 K.H.Zawawi, A.Kantarci, U.Schulze-Späte, T.Fujita, E.L.Batista, S.Amar, and T.E.Van Dyke (2010).
Moesin-induced signaling in response to lipopolysaccharide in macrophages.
  J Periodontal Res, 45, 589-601.  
20966971 M.C.Frame, H.Patel, B.Serrels, D.Lietha, and M.J.Eck (2010).
The FERM domain: organizing the structure and function of FAK.
  Nat Rev Mol Cell Biol, 11, 802-814.  
20855527 M.Gloerich, B.Ponsioen, M.J.Vliem, Z.Zhang, J.Zhao, M.R.Kooistra, L.S.Price, L.Ritsma, F.J.Zwartkruis, H.Rehmann, K.Jalink, and J.L.Bos (2010).
Spatial regulation of cyclic AMP-Epac1 signaling in cell adhesion by ERM proteins.
  Mol Cell Biol, 30, 5421-5431.  
20947018 P.R.Elliott, B.T.Goult, P.M.Kopp, N.Bate, J.G.Grossmann, G.C.Roberts, D.R.Critchley, and I.L.Barsukov (2010).
The Structure of the talin head reveals a novel extended conformation of the FERM domain.
  Structure, 18, 1289-1299.
PDB code: 3ivf
19884346 R.F.Hennigan, L.A.Foster, M.F.Chaiken, T.Mani, M.M.Gomes, A.B.Herr, and W.Ip (2010).
Fluorescence resonance energy transfer analysis of merlin conformational changes.
  Mol Cell Biol, 30, 54-67.  
20308985 R.G.Fehon, A.I.McClatchey, and A.Bretscher (2010).
Organizing the cell cortex: the role of ERM proteins.
  Nat Rev Mol Cell Biol, 11, 276-287.  
19345106 A.I.McClatchey, and R.G.Fehon (2009).
Merlin and the ERM proteins--regulators of receptor distribution and signaling at the cell cortex.
  Trends Cell Biol, 19, 198-206.  
19129194 D.J.Killock, M.Parsons, M.Zarrouk, S.M.Ameer-Beg, A.J.Ridley, D.O.Haskard, M.Zvelebil, and A.Ivetic (2009).
In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin.
  J Biol Chem, 284, 8833-8845.  
19664602 D.Zheng, E.Kurenova, D.Ucar, V.Golubovskaya, A.Magis, D.Ostrov, W.G.Cance, and S.N.Hochwald (2009).
Targeting of the protein interaction site between FAK and IGF-1R.
  Biochem Biophys Res Commun, 388, 301-305.  
19204146 J.J.Hao, Y.Liu, M.Kruhlak, K.E.Debell, B.L.Rellahan, and S.Shaw (2009).
Phospholipase C-mediated hydrolysis of PIP2 releases ERM proteins from lymphocyte membrane.
  J Cell Biol, 184, 451-462.  
19556303 J.L.Espinoza, H.Takamatsu, X.Lu, Z.Qi, and S.Nakao (2009).
Anti-moesin antibodies derived from patients with aplastic anemia stimulate monocytic cells to secrete TNF-alpha through an ERK1/2-dependent pathway.
  Int Immunol, 21, 913-923.  
19060919 L.Ren, S.H.Hong, J.Cassavaugh, T.Osborne, A.J.Chou, S.Y.Kim, R.Gorlick, S.M.Hewitt, and C.Khanna (2009).
The actin-cytoskeleton linker protein ezrin is regulated during osteosarcoma metastasis by PKC.
  Oncogene, 28, 792-802.  
19156760 M.Gruber-Olipitz, T.Ströbel, S.U.Kang, J.P.John, M.A.Grotzer, I.Slavc, and G.Lubec (2009).
Neurotrophin 3/TrkC-regulated proteins in the human medulloblastoma cell line DAOY.
  Electrophoresis, 30, 540-549.  
19388049 M.Y.Niv, K.Iida, R.Zheng, A.Horiguchi, R.Shen, and D.M.Nanus (2009).
Rational redesign of neutral endopeptidase binding to merlin and moesin proteins.
  Protein Sci, 18, 1042-1050.  
19190245 Q.Kang, Y.Yu, X.Pei, R.Hughes, S.Heck, X.Zhang, X.Guo, G.Halverson, N.Mohandas, and X.An (2009).
Cytoskeletal protein 4.1R negatively regulates T-cell activation by inhibiting the phosphorylation of LAT.
  Blood, 113, 6128-6137.  
19273615 R.Rollason, V.Korolchuk, C.Hamilton, M.Jepson, and G.Banting (2009).
A CD317/tetherin-RICH2 complex plays a critical role in the organization of the subapical actin cytoskeleton in polarized epithelial cells.
  J Cell Biol, 184, 721-736.  
19596566 U.Tepass (2009).
FERM proteins in animal morphogenesis.
  Curr Opin Genet Dev, 19, 357-367.  
19255446 Y.Yang, T.G.Baboolal, V.Siththanandan, M.Chen, M.L.Walker, P.J.Knight, M.Peckham, and J.R.Sellers (2009).
A FERM domain autoregulates Drosophila myosin 7a activity.
  Proc Natl Acad Sci U S A, 106, 4189-4194.  
18786395 A.Schulte, B.Stolp, A.Schönichen, O.Pylypenko, A.Rak, O.T.Fackler, and M.Geyer (2008).
The human formin FHOD1 contains a bipartite structure of FH3 and GTPase-binding domains required for activation.
  Structure, 16, 1313-1323.
PDB code: 3dad
18651636 C.D.Mintz, I.Carcea, D.G.McNickle, T.C.Dickson, Y.Ge, S.R.Salton, and D.L.Benson (2008).
ERM proteins regulate growth cone responses to Sema3A.
  J Comp Neurol, 510, 351-366.  
18768751 D.Komander, M.Patel, M.Laurin, N.Fradet, A.Pelletier, D.Barford, and J.F.Côté (2008).
An alpha-helical extension of the ELMO1 pleckstrin homology domain mediates direct interaction to DOCK180 and is critical in Rac signaling.
  Mol Biol Cell, 19, 4837-4851.
PDB code: 2vsz
18614051 E.Goksoy, Y.Q.Ma, X.Wang, X.Kong, D.Perera, E.F.Plow, and J.Qin (2008).
Structural basis for the autoinhibition of talin in regulating integrin activation.
  Mol Cell, 31, 124-133.  
17827228 G.Blin, E.Margeat, K.Carvalho, C.A.Royer, C.Roy, and C.Picart (2008).
Quantitative analysis of the binding of ezrin to large unilamellar vesicles containing phosphatidylinositol 4,5 bisphosphate.
  Biophys J, 94, 1021-1033.  
18997731 H.Larjava, E.F.Plow, and C.Wu (2008).
Kindlins: essential regulators of integrin signalling and cell-matrix adhesion.
  EMBO Rep, 9, 1203-1208.  
18725408 J.Austermann, A.R.Nazmi, C.Müller-Tidow, and V.Gerke (2008).
Characterization of the Ca2+ -regulated ezrin-S100P interaction and its role in tumor cell migration.
  J Biol Chem, 283, 29331-29340.  
18397735 J.Rosenblatt (2008).
Mitosis: moesin and the importance of being round.
  Curr Biol, 18, R292-R293.  
18480298 L.Zhu, J.Hatakeyama, C.Chen, A.Shastri, K.Poon, and J.G.Forte (2008).
Comparative study of ezrin phosphorylation among different tissues: more is good; too much is bad.
  Am J Physiol Cell Physiol, 295, C192-C202.  
18165225 M.Bouaouina, Y.Lad, and D.A.Calderwood (2008).
The N-terminal domains of talin cooperate with the phosphotyrosine binding-like domain to activate beta1 and beta3 integrins.
  J Biol Chem, 283, 6118-6125.  
18207738 P.Kunda, A.E.Pelling, T.Liu, and B.Baum (2008).
Moesin controls cortical rigidity, cell rounding, and spindle morphogenesis during mitosis.
  Curr Biol, 18, 91.  
17729253 S.T.Zuckerman, and W.J.Kao (2008).
LC/MS identification of 12 intracellular cytoskeletal and inflammatory proteins from monocytes adherent on surface-adsorbed fibronectin-derived peptides.
  J Biomed Mater Res A, 85, 513-529.  
18753140 T.Mori, K.Kitano, S.Terawaki, R.Maesaki, Y.Fukami, and T.Hakoshima (2008).
Structural basis for CD44 recognition by ERM proteins.
  J Biol Chem, 283, 29602-29612.
PDB code: 2zpy
18039098 A.I.McClatchey (2007).
Neurofibromatosis.
  Annu Rev Pathol, 2, 191-216.  
17003372 A.Kasirer-Friede, B.Moran, J.Nagrampa-Orje, K.Swanson, Z.M.Ruggeri, B.Schraven, B.G.Neel, G.Koretzky, and S.J.Shattil (2007).
ADAP is required for normal alphaIIbbeta3 activation by VWF/GP Ib-IX-V and other agonists.
  Blood, 109, 1018-1025.  
17605815 A.M.Burroughs, S.Balaji, L.M.Iyer, and L.Aravind (2007).
Small but versatile: the extraordinary functional and structural diversity of the beta-grasp fold.
  Biol Direct, 2, 18.  
16889625 A.W.Maresso, Q.Deng, M.S.Pereckas, B.T.Wakim, and J.T.Barbieri (2007).
Pseudomonas aeruginosa ExoS ADP-ribosyltransferase inhibits ERM phosphorylation.
  Cell Microbiol, 9, 97.  
17574028 D.Lietha, X.Cai, D.F.Ceccarelli, Y.Li, M.D.Schaller, and M.J.Eck (2007).
Structural basis for the autoinhibition of focal adhesion kinase.
  Cell, 129, 1177-1187.
PDB codes: 2j0j 2j0k 2j0l 2j0m
  17982027 G.G.Garcia, A.A.Sadighi Akha, and R.A.Miller (2007).
Age-related defects in moesin/ezrin cytoskeletal signals in mouse CD4 T cells.
  J Immunol, 179, 6403-6409.  
17044054 J.P.Taskinen, D.M.van Aalten, J.Knudsen, and R.K.Wierenga (2007).
High resolution crystal structures of unliganded and liganded human liver ACBP reveal a new mode of binding for the acyl-CoA ligand.
  Proteins, 66, 229-238.
PDB codes: 2cb8 2fj9
17548515 M.Curto, B.K.Cole, D.Lallemand, C.H.Liu, and A.I.McClatchey (2007).
Contact-dependent inhibition of EGFR signaling by Nf2/Merlin.
  J Cell Biol, 177, 893-903.  
17134719 Q.Li, M.R.Nance, R.Kulikauskas, K.Nyberg, R.Fehon, P.A.Karplus, A.Bretscher, and J.J.Tesmer (2007).
Self-masking in an intact ERM-merlin protein: an active role for the central alpha-helical domain.
  J Mol Biol, 365, 1446-1459.
PDB codes: 2i1j 2i1k
17916086 S.Béraud-Dufour, R.Gautier, C.Albiges-Rizo, P.Chardin, and E.Faurobert (2007).
Krit 1 interactions with microtubules and membranes are regulated by Rap1 and integrin cytoplasmic domain associated protein-1.
  FEBS J, 274, 5518-5532.  
17538024 S.Prag, M.Parsons, M.D.Keppler, S.M.Ameer-Beg, P.Barber, J.Hunt, A.J.Beavil, R.Calvert, M.Arpin, B.Vojnovic, and T.Ng (2007).
Activated ezrin promotes cell migration through recruitment of the GEF Dbl to lipid rafts and preferential downstream activation of Cdc42.
  Mol Biol Cell, 18, 2935-2948.  
17459884 S.Terawaki, K.Kitano, and T.Hakoshima (2007).
Structural basis for type II membrane protein binding by ERM proteins revealed by the radixin-neutral endopeptidase 24.11 (NEP) complex.
  J Biol Chem, 282, 19854-19862.
PDB code: 2yvc
18025306 T.Ilani, C.Khanna, M.Zhou, T.D.Veenstra, and A.Bretscher (2007).
Immune synapse formation requires ZAP-70 recruitment by ezrin and CD43 removal by moesin.
  J Cell Biol, 179, 733-746.  
17442573 T.Okada, L.You, and F.G.Giancotti (2007).
Shedding light on Merlin's wizardry.
  Trends Cell Biol, 17, 222-229.  
17891137 X.Tang, S.W.Jang, X.Wang, Z.Liu, S.M.Bahr, S.Y.Sun, D.Brat, D.H.Gutmann, and K.Ye (2007).
Akt phosphorylation regulates the tumour-suppressor merlin through ubiquitination and degradation.
  Nat Cell Biol, 9, 1199-1207.  
17635696 Y.Q.Ma, J.Qin, and E.F.Plow (2007).
Platelet integrin alpha(IIb)beta(3): activation mechanisms.
  J Thromb Haemost, 5, 1345-1352.  
17520274 Y.S.Mao, and H.L.Yin (2007).
Regulation of the actin cytoskeleton by phosphatidylinositol 4-phosphate 5 kinases.
  Pflugers Arch, 455, 5.  
16626485 A.J.Baines (2006).
A FERM-adjacent (FA) region defines a subset of the 4.1 superfamily and is a potential regulator of FERM domain function.
  BMC Genomics, 7, 85.  
16436990 B.A.Neff, D.B.Welling, E.Akhmametyeva, and L.S.Chang (2006).
The molecular biology of vestibular schwannomas: dissecting the pathogenic process at the molecular level.
  Otol Neurotol, 27, 197-208.  
16540524 B.Cha, M.Tse, C.Yun, O.Kovbasnjuk, S.Mohan, A.Hubbard, M.Arpin, and M.Donowitz (2006).
The NHE3 juxtamembrane cytoplasmic domain directly binds ezrin: dual role in NHE3 trafficking and mobility in the brush border.
  Mol Biol Cell, 17, 2661-2673.  
16377631 C.M.Grimsley, M.Lu, L.B.Haney, J.M.Kinchen, and K.S.Ravichandran (2006).
Characterization of a novel interaction between ELMO1 and ERM proteins.
  J Biol Chem, 281, 5928-5937.  
16221668 D.F.Ceccarelli, H.K.Song, F.Poy, M.D.Schaller, and M.J.Eck (2006).
Crystal structure of the FERM domain of focal adhesion kinase.
  J Biol Chem, 281, 252-259.
PDB codes: 2aeh 2al6
  16569237 D.L.Scott, G.Diez, and W.H.Goldmann (2006).
Protein-lipid interactions: correlation of a predictive algorithm for lipid-binding sites with three-dimensional structural data.
  Theor Biol Med Model, 3, 17.  
17178913 H.Chiba, N.Sakai, M.Murata, M.Osanai, T.Ninomiya, T.Kojima, and N.Sawada (2006).
The nuclear receptor hepatocyte nuclear factor 4alpha acts as a morphogen to induce the formation of microvilli.
  J Cell Biol, 175, 971-980.  
16616874 J.H.Hurley (2006).
Membrane binding domains.
  Biochim Biophys Acta, 1761, 805-811.  
16247459 J.Y.Lee, H.J.Moon, W.K.Lee, H.J.Chun, C.W.Han, Y.W.Jeon, Y.Lim, Y.H.Kim, T.P.Yao, K.H.Lee, T.Y.Jun, H.K.Rha, and J.K.Kang (2006).
Merlin facilitates ubiquitination and degradation of transactivation-responsive RNA-binding protein.
  Oncogene, 25, 1143-1152.  
  16582480 K.Kitano, F.Yusa, and T.Hakoshima (2006).
Structure of dimerized radixin FERM domain suggests a novel masking motif in C-terminal residues 295-304.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 62, 340-345.
PDB code: 2d2q
16368689 K.M.Abdi, P.J.Mohler, J.Q.Davis, and V.Bennett (2006).
Isoform specificity of ankyrin-B: a site in the divergent C-terminal domain is required for intramolecular association.
  J Biol Chem, 281, 5741-5749.  
16908865 L.P.Erwig, K.A.McPhilips, M.W.Wynes, A.Ivetic, A.J.Ridley, and P.M.Henson (2006).
Differential regulation of phagosome maturation in macrophages and dendritic cells mediated by Rho GTPases and ezrin-radixin-moesin (ERM) proteins.
  Proc Natl Acad Sci U S A, 103, 12825-12830.  
16352594 L.Stanasila, L.Abuin, D.Diviani, and S.Cotecchia (2006).
Ezrin directly interacts with the alpha1b-adrenergic receptor and plays a role in receptor recycling.
  J Biol Chem, 281, 4354-4363.  
16521120 M.E.Baser (2006).
The distribution of constitutional and somatic mutations in the neurofibromatosis 2 gene.
  Hum Mutat, 27, 297-306.  
16830345 S.J.Franco, M.A.Senetar, W.T.Simonson, A.Huttenlocher, and R.O.McCann (2006).
The conserved C-terminal I/LWEQ module targets Talin1 to focal adhesions.
  Cell Motil Cytoskeleton, 63, 563-581.  
16782899 S.Y.Chen, and H.C.Chen (2006).
Direct interaction of focal adhesion kinase (FAK) with Met is required for FAK to promote hepatocyte growth factor-induced cell invasion.
  Mol Cell Biol, 26, 5155-5167.  
16140532 A.D.Sousa, and R.E.Cheney (2005).
Myosin-X: a molecular motor at the cell's fingertips.
  Trends Cell Biol, 15, 533-539.  
16102045 B.I.Ratnikov, A.W.Partridge, and M.H.Ginsberg (2005).
Integrin activation by talin.
  J Thromb Haemost, 3, 1783-1790.  
15531580 H.Shiue, M.W.Musch, Y.Wang, E.B.Chang, and J.R.Turner (2005).
Akt2 phosphorylates ezrin to trigger NHE3 translocation and activation.
  J Biol Chem, 280, 1688-1695.  
16322781 J.S.Bennett (2005).
Structure and function of the platelet integrin alphaIIbbeta3.
  J Clin Invest, 115, 3363-3369.  
15647376 J.Srivastava, B.E.Elliott, D.Louvard, and M.Arpin (2005).
Src-dependent ezrin phosphorylation in adhesion-mediated signaling.
  Mol Biol Cell, 16, 1481-1490.  
16324214 K.Golovnina, A.Blinov, E.M.Akhmametyeva, L.V.Omelyanchuk, and L.S.Chang (2005).
Evolution and origin of merlin, the product of the Neurofibromatosis type 2 (NF2) tumor-suppressor gene.
  BMC Evol Biol, 5, 69.  
16086323 K.Itoh, M.Lisovsky, H.Hikasa, and S.Y.Sokol (2005).
Reorganization of actin cytoskeleton by FRIED, a Frizzled-8 associated protein tyrosine phosphatase.
  Dev Dyn, 234, 90.  
15611137 L.A.Cohen, and J.L.Guan (2005).
Residues within the first subdomain of the FERM-like domain in focal adhesion kinase are important in its regulation.
  J Biol Chem, 280, 8197-8207.  
15623525 L.Heiska, and O.Carpén (2005).
Src phosphorylates ezrin at tyrosine 477 and induces a phosphospecific association between ezrin and a kelch-repeat protein family member.
  J Biol Chem, 280, 10244-10252.  
15819698 M.Zhang, S.S.Bohlson, M.Dy, and A.J.Tenner (2005).
Modulated interaction of the ERM protein, moesin, with CD93.
  Immunology, 115, 63-73.  
15960311 N.Terada, N.Ohno, H.Yamakawa, O.Ohara, and S.Ohno (2005).
Topographical significance of membrane skeletal component protein 4.1 B in mammalian organs.
  Anat Sci Int, 80, 61-70.  
16151629 S.Fais, A.De Milito, and F.Lozupone (2005).
The role of FAS to ezrin association in FAS-mediated apoptosis.
  Apoptosis, 10, 941-947.  
15843435 S.H.Cant, and J.A.Pitcher (2005).
G protein-coupled receptor kinase 2-mediated phosphorylation of ezrin is required for G protein-coupled receptor-dependent reorganization of the actin cytoskeleton.
  Mol Biol Cell, 16, 3088-3099.  
15729356 S.Pust, H.Morrison, J.Wehland, A.S.Sechi, and P.Herrlich (2005).
Listeria monocytogenes exploits ERM protein functions to efficiently spread from cell to cell.
  EMBO J, 24, 1287-1300.  
15965491 T.Kinashi (2005).
Intracellular signalling controlling integrin activation in lymphocytes.
  Nat Rev Immunol, 5, 546-559.  
15869386 W.Cho, and R.V.Stahelin (2005).
Membrane-protein interactions in cell signaling and membrane trafficking.
  Annu Rev Biophys Biomol Struct, 34, 119-151.  
15178693 A.Ivetic, O.Florey, J.Deka, D.O.Haskard, A.Ager, and A.J.Ridley (2004).
Mutagenesis of the ezrin-radixin-moesin binding domain of L-selectin tail affects shedding, microvillar positioning, and leukocyte tethering.
  J Biol Chem, 279, 33263-33272.  
14704146 A.Iwase, R.Shen, D.Navarro, and D.M.Nanus (2004).
Direct binding of neutral endopeptidase 24.11 to ezrin/radixin/moesin (ERM) proteins competes with the interaction of CD44 with ERM proteins.
  J Biol Chem, 279, 11898-11905.  
15075368 A.Lee, and J.E.Treisman (2004).
Excessive Myosin activity in mbs mutants causes photoreceptor movement out of the Drosophila eye disc epithelium.
  Mol Biol Cell, 15, 3285-3295.  
14993232 B.T.Fievet, A.Gautreau, C.Roy, L.Del Maestro, P.Mangeat, D.Louvard, and M.Arpin (2004).
Phosphoinositide binding and phosphorylation act sequentially in the activation mechanism of ezrin.
  J Cell Biol, 164, 653-659.  
15183186 C.Polesello, and F.Payre (2004).
Small is beautiful: what flies tell us about ERM protein function in development.
  Trends Cell Biol, 14, 294-302.  
15277531 C.Steindler, Z.Li, M.Algarté, A.Alcover, V.Libri, J.Ragimbeau, and S.Pellegrini (2004).
Jamip1 (marlin-1) defines a family of proteins interacting with janus kinases and microtubules.
  J Biol Chem, 279, 43168-43177.  
15579689 D.P.Kiehart, J.D.Franke, M.K.Chee, R.A.Montague, T.L.Chen, J.Roote, and M.Ashburner (2004).
Drosophila crinkled, mutations of which disrupt morphogenesis and cause lethality, encodes fly myosin VIIA.
  Genetics, 168, 1337-1352.  
14676203 F.Lozupone, L.Lugini, P.Matarrese, F.Luciani, C.Federici, E.Iessi, P.Margutti, G.Stassi, W.Malorni, and S.Fais (2004).
Identification and relevance of the CD95-binding domain in the N-terminal region of ezrin.
  J Biol Chem, 279, 9199-9207.  
15156152 H.Zhang, J.S.Berg, Z.Li, Y.Wang, P.Lång, A.D.Sousa, A.Bhaskar, R.E.Cheney, and S.Strömblad (2004).
Myosin-X provides a motor-based link between integrins and the cytoskeleton.
  Nat Cell Biol, 6, 523-531.  
15123646 I.Behrmann, T.Smyczek, P.C.Heinrich, H.Schmitz-Van de Leur, W.Komyod, B.Giese, G.Müller-Newen, S.Haan, and C.Haan (2004).
Janus kinase (Jak) subcellular localization revisited: the exclusive membrane localization of endogenous Janus kinase 1 by cytokine receptor interaction uncovers the Jak.receptor complex to be equivalent to a receptor tyrosine kinase.
  J Biol Chem, 279, 35486-35493.  
15362227 I.D.Campbell, and M.H.Ginsberg (2004).
The talin-tail interaction places integrin activation on FERM ground.
  Trends Biochem Sci, 29, 429-435.  
15039356 I.Iontcheva, S.Amar, K.H.Zawawi, A.Kantarci, and T.E.Van Dyke (2004).
Role for moesin in lipopolysaccharide-stimulated signal transduction.
  Infect Immun, 72, 2312-2320.  
15169899 J.M.Dunty, V.Gabarra-Niecko, M.L.King, D.F.Ceccarelli, M.J.Eck, and M.D.Schaller (2004).
FERM domain interaction promotes FAK signaling.
  Mol Cell Biol, 24, 5353-5368.  
15138281 K.J.Ralston, S.L.Hird, X.Zhang, J.L.Scott, B.Jin, R.F.Thorne, M.C.Berndt, A.W.Boyd, and G.F.Burns (2004).
The LFA-1-associated molecule PTA-1 (CD226) on T cells forms a dynamic molecular complex with protein 4.1G and human discs large.
  J Biol Chem, 279, 33816-33828.  
15313460 K.P.Hoeflich, and M.Ikura (2004).
Radixin: cytoskeletal adopter and signaling protein.
  Int J Biochem Cell Biol, 36, 2131-2136.  
15143187 L.S.Argetsinger, J.L.Kouadio, H.Steen, A.Stensballe, O.N.Jensen, and C.Carter-Su (2004).
Autophosphorylation of JAK2 on tyrosines 221 and 570 regulates its activity.
  Mol Cell Biol, 24, 4955-4967.  
14672952 M.S.Sozio, M.A.Mathis, J.A.Young, S.Wälchli, L.A.Pitcher, P.C.Wrage, B.Bartók, A.Campbell, J.D.Watts, R.Aebersold, R.H.Van Huijsduijnen, and N.S.van Oers (2004).
PTPH1 is a predominant protein-tyrosine phosphatase capable of interacting with and dephosphorylating the T cell receptor zeta subunit.
  J Biol Chem, 279, 7760-7769.  
14634021 S.Kloeker, M.B.Major, D.A.Calderwood, M.H.Ginsberg, D.A.Jones, and M.C.Beckerle (2004).
The Kindler syndrome protein is regulated by transforming growth factor-beta and involved in integrin-mediated adhesion.
  J Biol Chem, 279, 6824-6833.  
15195146 V.Gervais, V.Lamour, A.Jawhari, F.Frindel, E.Wasielewski, S.Dubaele, J.M.Egly, J.C.Thierry, B.Kieffer, and A.Poterszman (2004).
TFIIH contains a PH domain involved in DNA nucleotide excision repair.
  Nat Struct Mol Biol, 11, 616-622.
PDB code: 1pfj
15152196 V.Ramesh (2004).
Merlin and the ERM proteins in Schwann cells, neurons and growth cones.
  Nat Rev Neurosci, 5, 462-470.  
15211576 Y.Zhan, D.He, P.E.Newburger, and G.W.Zhou (2004).
p47(phox) PX domain of NADPH oxidase targets cell membrane via moesin-mediated association with the actin cytoskeleton.
  J Cell Biochem, 92, 795-809.  
12471027 A.Gautreau, B.T.Fievet, E.Brault, C.Antony, A.Houdusse, D.Louvard, and M.Arpin (2003).
Isolation and characterization of an aggresome determinant in the NF2 tumor suppressor.
  J Biol Chem, 278, 6235-6242.  
14668818 A.I.McClatchey (2003).
Merlin and ERM proteins: unappreciated roles in cancer development?
  Nat Rev Cancer, 3, 877-883.  
12878601 B.Giese, C.K.Au-Yeung, A.Herrmann, S.Diefenbach, C.Haan, A.Küster, S.B.Wortmann, C.Roderburg, P.C.Heinrich, I.Behrmann, and G.Müller-Newen (2003).
Long term association of the cytokine receptor gp130 and the Janus kinase Jak1 revealed by FRAP analysis.
  J Biol Chem, 278, 39205-39213.  
12900915 C.D.Mintz, T.C.Dickson, M.L.Gripp, S.R.Salton, and D.L.Benson (2003).
ERMs colocalize transiently with L1 during neocortical axon outgrowth.
  J Comp Neurol, 464, 438-448.  
12471164 H.L.Yin, and P.A.Janmey (2003).
Phosphoinositide regulation of the actin cytoskeleton.
  Annu Rev Physiol, 65, 761-789.  
12769842 H.S.Yang, and P.W.Hinds (2003).
Increased ezrin expression and activation by CDK5 coincident with acquisition of the senescent phenotype.
  Mol Cell, 11, 1163-1176.  
14580336 J.L.Kissil, E.W.Wilker, K.C.Johnson, M.S.Eckman, M.B.Yaffe, and T.Jacks (2003).
Merlin, the product of the Nf2 tumor suppressor gene, is an inhibitor of the p21-activated kinase, Pak1.
  Mol Cell, 12, 841-849.  
12554651 K.Hamada, T.Shimizu, S.Yonemura, S.Tsukita, S.Tsukita, and T.Hakoshima (2003).
Structural basis of adhesion-molecule recognition by ERM proteins revealed by the crystal structure of the radixin-ICAM-2 complex.
  EMBO J, 22, 502-514.
PDB code: 1j19
14529273 K.P.Hoeflich, S.Tsukita, L.Hicks, C.M.Kay, S.Tsukita, and M.Ikura (2003).
Insights into a single rod-like helix in activated radixin required for membrane-cytoskeletal cross-linking.
  Biochemistry, 42, 11634-11641.  
14653806 K.S.Erdmann (2003).
The protein tyrosine phosphatase PTP-Basophil/Basophil-like. Interacting proteins and molecular functions.
  Eur J Biochem, 270, 4789-4798.  
12808036 M.Koltzscher, C.Neumann, S.König, and V.Gerke (2003).
Ca2+-dependent binding and activation of dormant ezrin by dimeric S100P.
  Mol Biol Cell, 14, 2372-2384.  
12573235 R.C.Liddington, L.A.Bankston, and J.M.de Pereda (2003).
Cell adhesion: a FERM grasp of membrane dynamics.
  Curr Biol, 13, R94-R95.  
12840026 R.Zhou, X.Cao, C.Watson, Y.Miao, Z.Guo, J.G.Forte, and X.Yao (2003).
Characterization of protein kinase A-mediated phosphorylation of ezrin in gastric parietal cell activation.
  J Biol Chem, 278, 35651-35659.  
14521927 S.Chauhan, R.Pandey, J.F.Way, T.C.Sroka, M.C.Demetriou, S.Kunz, A.E.Cress, D.W.Mount, and R.L.Miesfeld (2003).
Androgen regulation of the human FERM domain encoding gene EHM2 in a cell model of steroid-induced differentiation.
  Biochem Biophys Res Commun, 310, 421-432.  
12517699 S.J.Winder (2003).
Structural insights into actin-binding, branching and bundling proteins.
  Curr Opin Cell Biol, 15, 14-22.  
12711360 T.A.Martin, G.Harrison, R.E.Mansel, and W.G.Jiang (2003).
The role of the CD44/ezrin complex in cancer metastasis.
  Crit Rev Oncol Hematol, 46, 165-186.  
12429733 W.J.Smith, N.Nassar, A.Bretscher, R.A.Cerione, and P.A.Karplus (2003).
Structure of the active N-terminal domain of Ezrin. Conformational and mobility changes identify keystone interactions.
  J Biol Chem, 278, 4949-4956.
PDB code: 1ni2
12154370 A.Bretscher, K.Edwards, and R.G.Fehon (2002).
ERM proteins and merlin: integrators at the cell cortex.
  Nat Rev Mol Cell Biol, 3, 586-599.  
11792551 A.Gautreau, D.Louvard, and M.Arpin (2002).
ERM proteins and NF2 tumor suppressor: the Yin and Yang of cortical actin organization and cell growth signaling.
  Curr Opin Cell Biol, 14, 104-109.  
11856822 B.S.Kang, D.R.Cooper, Y.Devedjiev, U.Derewenda, and Z.S.Derewenda (2002).
The structure of the FERM domain of merlin, the neurofibromatosis type 2 gene product.
  Acta Crystallogr D Biol Crystallogr, 58, 381-391.
PDB code: 1h4r
12360288 C.Polesello, I.Delon, P.Valenti, P.Ferrer, and F.Payre (2002).
Dmoesin controls actin-based cell shape and polarity during Drosophila melanogaster oogenesis.
  Nat Cell Biol, 4, 782-789.  
11932255 D.A.Calderwood, B.Yan, J.M.de Pereda, B.G.Alvarez, Y.Fujioka, R.C.Liddington, and M.H.Ginsberg (2002).
The phosphotyrosine binding-like domain of talin activates integrins.
  J Biol Chem, 277, 21749-21758.  
12383800 F.R.Maxfield (2002).
Plasma membrane microdomains.
  Curr Opin Cell Biol, 14, 483-487.  
12422219 G.Di Paolo, L.Pellegrini, K.Letinic, G.Cestra, R.Zoncu, S.Voronov, S.Chang, J.Guo, M.R.Wenk, and P.De Camilli (2002).
Recruitment and regulation of phosphatidylinositol phosphate kinase type 1 gamma by the FERM domain of talin.
  Nature, 420, 85-89.  
11782491 J.L.Kissil, K.C.Johnson, M.S.Eckman, and T.Jacks (2002).
Merlin phosphorylation by p21-activated kinase 2 and effects of phosphorylation on merlin localization.
  J Biol Chem, 277, 10394-10399.  
12011064 J.S.Greiser, C.Stross, P.C.Heinrich, I.Behrmann, and H.M.Hermanns (2002).
Orientational constraints of the gp130 intracellular juxtamembrane domain for signaling.
  J Biol Chem, 277, 26959-26965.  
12032545 J.W.Legg, C.A.Lewis, M.Parsons, T.Ng, and C.M.Isacke (2002).
A novel PKC-regulated mechanism controls CD44 ezrin association and directional cell motility.
  Nat Cell Biol, 4, 399-407.  
12142282 M.A.Pufall, and B.J.Graves (2002).
Autoinhibitory domains: modular effectors of cellular regulation.
  Annu Rev Cell Dev Biol, 18, 421-462.  
12391143 M.Toutant, A.Costa, J.M.Studler, G.Kadaré, M.Carnaud, and J.A.Girault (2002).
Alternative splicing controls the mechanisms of FAK autophosphorylation.
  Mol Cell Biol, 22, 7731-7743.  
12032337 N.P.Hoe, R.M.Ireland, F.R.DeLeo, B.B.Gowen, D.W.Dorward, J.M.Voyich, M.Liu, E.H.Burns, D.M.Culnan, A.Bretscher, and J.M.Musser (2002).
Insight into the molecular basis of pathogen abundance: group A Streptococcus inhibitor of complement inhibits bacterial adherence and internalization into human cells.
  Proc Natl Acad Sci U S A, 99, 7646-7651.  
11792550 P.J.Renfranz, and M.C.Beckerle (2002).
Doing (F/L)PPPPs: EVH1 domains and their proline-rich partners in cell polarity and migration.
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12213832 R.C.Liddington, and M.H.Ginsberg (2002).
Integrin activation takes shape.
  J Cell Biol, 158, 833-839.  
11891121 S.P.Denker, and D.L.Barber (2002).
Ion transport proteins anchor and regulate the cytoskeleton.
  Curr Opin Cell Biol, 14, 214-220.  
11907041 S.Pokutta, F.Drees, Y.Takai, W.J.Nelson, and W.I.Weis (2002).
Biochemical and structural definition of the l-afadin- and actin-binding sites of alpha-catenin.
  J Biol Chem, 277, 18868-18874.
PDB code: 1l7c
11959505 S.Pokutta, and W.I.Weis (2002).
The cytoplasmic face of cell contact sites.
  Curr Opin Struct Biol, 12, 255-262.  
11756419 T.Shimizu, A.Seto, N.Maita, K.Hamada, S.Tsukita, S.Tsukita, and T.Hakoshima (2002).
Structural basis for neurofibromatosis type 2. Crystal structure of the merlin FERM domain.
  J Biol Chem, 277, 10332-10336.
PDB code: 1isn
12445270 V.Das, B.Nal, A.Roumier, V.Meas-Yedid, C.Zimmer, J.C.Olivo-Marin, P.Roux, P.Ferrier, A.Dautry-Varsat, and A.Alcover (2002).
Membrane-cytoskeleton interactions during the formation of the immunological synapse and subsequent T-cell activation.
  Immunol Rev, 189, 123-135.  
12136155 W.J.Smith, and R.A.Cerione (2002).
Crystallization and preliminary crystallographic analysis of the ezrin FERM domain.
  Acta Crystallogr D Biol Crystallogr, 58, 1359-1361.  
11285285 D.Reczek, and A.Bretscher (2001).
Identification of EPI64, a TBC/rabGAP domain-containing microvillar protein that binds to the first PDZ domain of EBP50 and E3KARP.
  J Cell Biol, 153, 191-206.  
11316791 H.Morrison, L.S.Sherman, J.Legg, F.Banine, C.Isacke, C.A.Haipek, D.H.Gutmann, H.Ponta, and P.Herrlich (2001).
The NF2 tumor suppressor gene product, merlin, mediates contact inhibition of growth through interactions with CD44.
  Genes Dev, 15, 968-980.  
11500444 I.Simonovic, M.Arpin, A.Koutsouris, H.J.Falk-Krzesinski, and G.Hecht (2001).
Enteropathogenic Escherichia coli activates ezrin, which participates in disruption of tight junction barrier function.
  Infect Immun, 69, 5679-5688.  
11248547 J.H.Hurley, and T.Meyer (2001).
Subcellular targeting by membrane lipids.
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11445584 J.K.Klarlund, J.Holik, A.Chawla, J.G.Park, J.Buxton, and M.P.Czech (2001).
Signaling complexes of the FERM domain-containing protein GRSP1 bound to ARF exchange factor GRP1.
  J Biol Chem, 276, 40065-40070.  
11779507 L.J.Huang, S.N.Constantinescu, and H.F.Lodish (2001).
The N-terminal domain of Janus kinase 2 is required for Golgi processing and cell surface expression of erythropoietin receptor.
  Mol Cell, 8, 1327-1338.  
11684412 L.S.Sherman, and D.H.Gutmann (2001).
Merlin: hanging tumor suppression on the Rac.
  Trends Cell Biol, 11, 442-444.  
11481332 R.Prekeris, J.M.Davies, and R.H.Scheller (2001).
Identification of a novel Rab11/25 binding domain present in Eferin and Rip proteins.
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11239466 S.N.Constantinescu, L.J.Huang, H.Nam, and H.F.Lodish (2001).
The erythropoietin receptor cytosolic juxtamembrane domain contains an essential, precisely oriented, hydrophobic motif.
  Mol Cell, 7, 377-385.  
11387207 T.Ng, M.Parsons, W.E.Hughes, J.Monypenny, D.Zicha, A.Gautreau, M.Arpin, S.Gschmeissner, P.J.Verveer, P.I.Bastiaens, and P.J.Parker (2001).
Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motility.
  EMBO J, 20, 2723-2741.  
11590013 V.Niggli (2001).
Structural properties of lipid-binding sites in cytoskeletal proteins.
  Trends Biochem Sci, 26, 604-611.  
11741532 Y.J.Zhou, M.Chen, N.A.Cusack, L.H.Kimmel, K.S.Magnuson, J.G.Boyd, W.Lin, J.L.Roberts, A.Lengi, R.H.Buckley, R.L.Geahlen, F.Candotti, M.Gadina, P.S.Changelian, and J.J.O'Shea (2001).
Unexpected effects of FERM domain mutations on catalytic activity of Jak3: structural implication for Janus kinases.
  Mol Cell, 8, 959-969.  
11224681 Y.Takakuwa (2001).
Regulation of red cell membrane protein interactions: implications for red cell function.
  Curr Opin Hematol, 8, 80-84.  
11031232 A.Bretscher, D.Chambers, R.Nguyen, and D.Reczek (2000).
ERM-Merlin and EBP50 protein families in plasma membrane organization and function.
  Annu Rev Cell Dev Biol, 16, 113-143.  
10893267 A.Gautreau, D.Louvard, and M.Arpin (2000).
Morphogenic effects of ezrin require a phosphorylation-induced transition from oligomers to monomers at the plasma membrane.
  J Cell Biol, 150, 193-203.  
11086008 C.Barret, C.Roy, P.Montcourrier, P.Mangeat, and V.Niggli (2000).
Mutagenesis of the phosphatidylinositol 4,5-bisphosphate (PIP(2)) binding site in the NH(2)-terminal domain of ezrin correlates with its altered cellular distribution.
  J Cell Biol, 151, 1067-1080.  
10970850 C.Tran Quang, A.Gautreau, M.Arpin, and R.Treisman (2000).
Ezrin function is required for ROCK-mediated fibroblast transformation by the Net and Dbl oncogenes.
  EMBO J, 19, 4565-4576.  
11114512 J.H.Hurley, Y.Tsujishita, and M.A.Pearson (2000).
Floundering about at cell membranes: a structural view of phospholipid signaling.
  Curr Opin Struct Biol, 10, 737-743.  
10970839 K.Hamada, T.Shimizu, T.Matsui, S.Tsukita, and T.Hakoshima (2000).
Structural basis of the membrane-targeting and unmasking mechanisms of the radixin FERM domain.
  EMBO J, 19, 4449-4462.
PDB codes: 1gc6 1gc7
11163215 S.P.Denker, D.C.Huang, J.Orlowski, H.Furthmayr, and D.L.Barber (2000).
Direct binding of the Na--H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation.
  Mol Cell, 6, 1425-1436.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.