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PDBsum entry 1ebv

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protein ligands links
Oxidoreductase PDB id
1ebv
Jmol
Contents
Protein chain
551 a.a. *
Ligands
NAG ×2
NAG-NAG
SCL
HEM
* Residue conservation analysis
PDB id:
1ebv
Name: Oxidoreductase
Title: Ovine pghs-1 complexed with salicyl hydroxamic acid
Structure: Prostaglandin h2 synthase. Chain: a. Ec: 1.14.99.1
Source: Ovis aries. Sheep. Organism_taxid: 9940. Organ: seminal vesicles
Resolution:
3.20Å     R-factor:   0.218     R-free:   0.248
Authors: P.J.Loll,C.T.Sharkey,S.J.O'Connor,D.J.Fitzgerald
Key ref: P.J.Loll et al. (2001). O-acetylsalicylhydroxamic acid, a novel acetylating inhibitor of prostaglandin H2 synthase: structural and functional characterization of enzyme-inhibitor interactions. Mol Pharmacol, 60, 1407-1413. PubMed id: 11723249
Date:
24-Jan-00     Release date:   24-Feb-00    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P05979  (PGH1_SHEEP) -  Prostaglandin G/H synthase 1
Seq:
Struc:
 
Seq:
Struc:
600 a.a.
551 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.1.14.99.1  - Prostaglandin-endoperoxide synthase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Arachidonate + AH2 + 2 O2 = prostaglandin H2 + A + H2O
Arachidonate
Bound ligand (Het Group name = HEM)
matches with 51.16% similarity
+ AH(2)
+ 2 × O(2)
= prostaglandin H(2)
+
+ H(2)O
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   8 terms 
  Biological process     oxidation-reduction process   10 terms 
  Biochemical function     oxidoreductase activity     6 terms  

 

 
    reference    
 
 
Mol Pharmacol 60:1407-1413 (2001)
PubMed id: 11723249  
 
 
O-acetylsalicylhydroxamic acid, a novel acetylating inhibitor of prostaglandin H2 synthase: structural and functional characterization of enzyme-inhibitor interactions.
P.J.Loll, C.T.Sharkey, S.J.O'Connor, C.M.Dooley, E.O'Brien, M.Devocelle, K.B.Nolan, B.S.Selinsky, D.J.Fitzgerald.
 
  ABSTRACT  
 
Aspirin is unique among clinically used nonsteroidal antiinflammatory drugs in that it irreversibly inactivates prostaglandin (PG) H2 synthase (PGHS) via acetylation of an active-site serine residue. We report the synthesis and characterization of a novel acetylating agent, O-acetylsalicylhydroxamic acid (AcSHA), which inhibits PGE2 synthesis in vivo and blocks the cyclooxygenase activity of PGHS in vitro. AcSHA requires the presence of the active-site residue Ser-529 to be active against human PGHS-1; the S529A mutant is resistant to inactivation by the inhibitor. Analysis of PGHS inactivation by AcSHA, coupled with the X-ray crystal structure of the complex of ovine PGHS-1 with AcSHA, confirms that the inhibitor elicits its effects via acetylation of Ser-529 in the cyclooxygenase active site. The crystal structure reveals an intact inhibitor molecule bound in the enzyme's cyclooxygenase active-site channel, hydrogen bonding with Arg-119 of the enzyme. The structure-activity profile of AcSHA can be rationalized in terms of the crystal structure of the enzyme-ligand complex. AcSHA may prove useful as a lead compound to facilitate the development of new acetylating inhibitors.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20196542 R.S.Deeb, C.Cheung, T.Nuriel, B.D.Lamon, R.K.Upmacis, S.S.Gross, and D.P.Hajjar (2010).
Physical evidence for substrate binding in preventing cyclooxygenase inactivation under nitrative stress.
  J Am Chem Soc, 132, 3914-3922.  
19433337 C.E.Rogge, W.Liu, R.J.Kulmacz, and A.L.Tsai (2009).
Peroxide-induced radical formation at TYR385 and TYR504 in human PGHS-1.
  J Inorg Biochem, 103, 912-922.  
19326386 M.Scholz, K.Bensdorf, R.Gust, and E.Hey-Hawkins (2009).
Asborin: the carbaborane analogue of aspirin.
  ChemMedChem, 4, 746-748.  
16211102 J.Lee, A.J.Chubb, E.Moman, B.M.McLoughlin, C.T.Sharkey, J.G.Kelly, K.B.Nolan, M.Devocelle, and D.J.Fitzgerald (2005).
Parallel synthesis and in vitro activity of novel anthranilic hydroxamate-based inhibitors of the prostaglandin H2 synthase peroxidase activity.
  Org Biomol Chem, 3, 3678-3685.  
17191953 R.G.Huff, E.Bayram, H.Tan, S.T.Knutson, M.H.Knaggs, A.B.Richon, P.Santago, and J.S.Fetrow (2005).
Chemical and structural diversity in cyclooxygenase protein active sites.
  Chem Biodivers, 2, 1533-1552.  
15289285 D.Taubert, R.Berkels, N.Grosser, H.Schröder, D.Gründemann, and E.Schömig (2004).
Aspirin induces nitric oxide release from vascular endothelium: a novel mechanism of action.
  Br J Pharmacol, 143, 159-165.  
12574066 R.M.Garavito, and A.M.Mulichak (2003).
The structure of mammalian cyclooxygenases.
  Annu Rev Biophys Biomol Struct, 32, 183-206.  
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