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PDBsum entry 1e4i

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protein ligands links
Hydrolase PDB id
1e4i
Jmol
Contents
Protein chain
447 a.a. *
Ligands
G2F
NFG
Waters ×234
* Residue conservation analysis
PDB id:
1e4i
Name: Hydrolase
Title: 2-deoxy-2-fluoro-beta-d-glucosyl/enzyme intermediate complex of the beta-glucosidase from bacillus polymyxa
Structure: Beta-glucosidase. Chain: a. Engineered: yes. Mutation: yes
Source: Bacillus polymyxa. Organism_taxid: 1406. Atcc: 842. Plasmid: puc derivative. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Homo-Octamer (from PDB file)
Resolution:
2.00Å     R-factor:   0.210     R-free:   0.250
Authors: J.Sanz-Aparicio,B.Gonzalez,J.A.Hermoso,J.C.Arribas,F.J.Canad J.Polaina
Key ref: J.Sanz-Aparicio et al. (1998). Structural basis of increased resistance to thermal denaturation induced by single amino acid substitution in the sequence of beta-glucosidase A from Bacillus polymyxa. Proteins, 33, 567-576. PubMed id: 9849940
Date:
06-Jul-00     Release date:   05-Jul-01    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P22073  (BGLA_PAEPO) -  Beta-glucosidase A
Seq:
Struc:
448 a.a.
447 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.2.1.21  - Beta-glucosidase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     metabolic process   4 terms 
  Biochemical function     hydrolase activity     4 terms  

 

 
Proteins 33:567-576 (1998)
PubMed id: 9849940  
 
 
Structural basis of increased resistance to thermal denaturation induced by single amino acid substitution in the sequence of beta-glucosidase A from Bacillus polymyxa.
J.Sanz-Aparicio, J.A.Hermoso, M.Martínez-Ripoll, B.González, C.López-Camacho, J.Polaina.
 
  ABSTRACT  
 
The increasing development of the biotechnology industry demands the design of enzymes suitable to be used in conditions that often require broad resistance against adverse conditions. beta-glucosidase A from Bacillus polymyxa is an interesting model for studies of protein engineering. This is a well-characterized enzyme, belonging to glycosyl hydrolase family 1. Its natural substrate is cellobiose, but is also active against various artificial substrates. In its native state has an octameric structure. Its subunit conserves the general (alpha/beta)8 barrel topology of its family, with the active site being in a cavity defined along the axis of the barrel. Using random-mutagenesis, we have identified several mutations enhancing its stability and it was found that one them, the E96K substitution, involved structural changes. The crystal structure of this mutant has been determined by X-ray diffraction and compared with the native structure. The only difference founded between both structures is a new ion pair linking Lys96 introduced at the N-terminus of helix alpha2, to Asp28, located in one of the loops surrounding the active-site cavity. The new ion pair binds two segments of the chain that are distant in sequence and, therefore, this favorable interaction must exert a determinant influence in stabilizing the tertiary structure. Furthermore, analysis of the crystallographic isotropic temperature factors reveals that, as a direct consequence of the introduced ion pair, an unexpected decreased mobility of secondary structure units of the barrel which are proximal to the site of mutation is observed. However, this effect is observed only in the surrounding of one of the partners forming the salt bridge and not around the other. These results show that far-reaching effects can be achieved by a single amino acid replacement within the protein structure. Consequently, the identification and combination of a few single substitutions affecting stability may be sufficient to obtain a highly resistant enzyme, suitable to be used under extreme conditions.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
  10788490 G.Gonzalez-Blasco, J.Sanz-Aparicio, B.Gonzalez, J.A.Hermoso, and J.Polaina (2000).
Directed evolution of beta -glucosidase A from Paenibacillus polymyxa to thermal resistance.
  J Biol Chem, 275, 13708-13712.  
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