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Toxin PDB id
1dw5
Jmol
Contents
Protein chain
26 a.a.
PDB id:
1dw5
Name: Toxin
Title: Nmr structure of omega-conotoxin mviia: no constraints on disulphide bridges
Structure: Omega-conotoxin mviia. Chain: a. Engineered: yes
Source: Synthetic: yes. Other_details: this peptide was chemically synthesized. The sequence of this peptide naturally occurs in conus magus (magus cone)
NMR struc: 25 models
Authors: R.A.Atkinson,B.Kieffer,A.Dejaegere,F.Sirockin,J.-F.Lefevre
Key ref:
R.A.Atkinson et al. (2000). Structural and dynamic characterization of omega-conotoxin MVIIA: the binding loop exhibits slow conformational exchange. Biochemistry, 39, 3908-3919. PubMed id: 10747778 DOI: 10.1021/bi992651h
Date:
24-Jan-00     Release date:   01-Mar-00    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P05484  (CO17A_CONMA) -  Omega-conotoxin MVIIA
Seq:
Struc: 		71 a.a.
26 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     pathogenesis   1 term 
  Biochemical function     ion channel inhibitor activity     1 term  

 

 
DOI no: 10.1021/bi992651h Biochemistry 39:3908-3919 (2000)
PubMed id: 10747778  
 
 
Structural and dynamic characterization of omega-conotoxin MVIIA: the binding loop exhibits slow conformational exchange.
R.A.Atkinson, B.Kieffer, A.Dejaegere, F.Sirockin, J.F.Lefèvre.
 
  ABSTRACT  
 
omega-Conotoxin MVIIA is a 25-residue, disulfide-bridged polypeptide from the venom of the sea snail Conus magus that binds to neuronal N-type calcium channels. It forms a compact folded structure, presenting a loop between Cys8 and Cys15 that contains a set of residues critical for its binding. The loop does not have a unique defined structure, nor is it intrinsically flexible. Broadening of a subset of resonances in the NMR spectrum at low temperature, anomalous temperature dependence of the chemical shifts of some resonances, and exchange contributions to J(0) from (13)C relaxation measurements reveal that conformational exchange affects the residues in this loop. The effects of this exchange on the calculated structure of omega-conotoxin MVIIA are discussed. The exchange appears to be associated with a change in the conformation of the disulfide bridge Cys8-Cys20. The implications for the use of the omega-conotoxins as a scaffold for carrying other functions is discussed.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19707262 H.S.Smith, and T.R.Deer (2009).
Safety and efficacy of intrathecal ziconotide in the management of severe chronic pain.
  Ther Clin Risk Manag, 5, 521-534.  
19165896 N.L.Daly, and D.J.Craik (2009).
Structural studies of conotoxins.
  IUBMB Life, 61, 144-150.  
19096380 T.F.Molinski, D.S.Dalisay, S.L.Lievens, and J.P.Saludes (2009).
Drug development from marine natural products.
  Nat Rev Drug Discov, 8, 69-85.  
18831583 A.Walewska, J.J.Skalicky, D.R.Davis, M.M.Zhang, E.Lopez-Vera, M.Watkins, T.S.Han, D.Yoshikami, B.M.Olivera, and G.Bulaj (2008).
NMR-based mapping of disulfide bridges in cysteine-rich peptides: application to the mu-conotoxin SxIIIA.
  J Am Chem Soc, 130, 14280-14286.  
17961068 G.Bulaj, and B.M.Olivera (2008).
Folding of conotoxins: formation of the native disulfide bridges during chemical synthesis and biosynthesis of conus peptides.
  Antioxid Redox Signal, 10, 141-156.  
14976206 L.Volpon, H.Lamthanh, J.Barbier, N.Gilles, J.Molgó, A.Ménez, and J.M.Lancelin (2004).
NMR solution structures of delta-conotoxin EVIA from Conus ermineus that selectively acts on vertebrate neuronal Na+ channels.
  J Biol Chem, 279, 21356-21366.
PDB codes: 1g1p 1g1z
15044438 N.L.Daly, J.A.Ekberg, L.Thomas, D.J.Adams, R.J.Lewis, and D.J.Craik (2004).
Structures of muO-conotoxins from Conus marmoreus. I nhibitors of tetrodotoxin (TTX)-sensitive and TTX-resistant sodium channels in mammalian sensory neurons.
  J Biol Chem, 279, 25774-25782.
PDB code: 1rmk
12557184 C.González, J.L.Neira, S.Ventura, S.Bronsoms, M.Rico, and F.X.Avilés (2003).
Structure and dynamics of the potato carboxypeptidase inhibitor by 1H and 15N NMR.
  Proteins, 50, 410-422.
PDB code: 1h20
11876659 M.Price-Carter, G.Bulaj, and D.P.Goldenberg (2002).
Initial disulfide formation steps in the folding of an omega-conotoxin.
  Biochemistry, 41, 3507-3519.  
11344322 D.P.Goldenberg, R.E.Koehn, D.E.Gilbert, and G.Wagner (2001).
Solution structure and backbone dynamics of an omega-conotoxin precursor.
  Protein Sci, 10, 538-550.
PDB code: 1feo
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.