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Hydrolase(carboxylic esterase)
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PDB id
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1crl
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Contents |
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* Residue conservation analysis
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Enzyme class:
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E.C.3.1.1.3
- Triacylglycerol lipase.
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Reaction:
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Triacylglycerol + H2O = diacylglycerol + a carboxylate
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Triacylglycerol
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+
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H(2)O
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=
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diacylglycerol
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+
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carboxylate
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Gene Ontology (GO) functional annotation
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Biological process
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lipid catabolic process
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1 term
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Biochemical function
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hydrolase activity
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2 terms
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J Biol Chem
268:12843-12847
(1993)
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PubMed id:
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Insights into interfacial activation from an open structure of Candida rugosa lipase.
|
|
P.Grochulski,
Y.Li,
J.D.Schrag,
F.Bouthillier,
P.Smith,
D.Harrison,
B.Rubin,
M.Cygler.
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ABSTRACT
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The structure of the Candida rugosa lipase determined at 2.06-A resolution
reveals a conformation with a solvent-accessible active site. Comparison with
the crystal structure of the homologous lipase from Geotrichum candidum, in
which the active site is covered by surface loops and is inaccessible from the
solvent, shows that the largest structural differences occur in the vicinity of
the active site. Three loops in this region differ significantly in
conformation, and the interfacial activation of these lipases is likely to be
associated with conformational rearrangements of these loops. The
"open" structure provides a new image of the substrate binding region
and active site access, which is different from that inferred from the structure
of the "closed" form of the G. candidum lipase.
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Literature references that cite this PDB file's key reference
|
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| |
PubMed id
|
|
Reference
|
|
|
|
|
|
J.P.Fuciños González,
G.Bassani,
B.Farruggia,
G.A.Picó,
L.Pastrana Castro,
and
M.L.Rua
(2011).
Conformational flexibility of lipase lip1 from Candida rugosa studied by electronic spectroscopies and thermodynamic approaches.
|
| |
Protein J, 30,
77-83.
|
|
|
|
|
|
|
X.Zheng,
X.Chu,
W.Zhang,
N.Wu,
and
Y.Fan
(2011).
A novel cold-adapted lipase from Acinetobacter sp. XMZ-26: gene cloning and characterisation.
|
| |
Appl Microbiol Biotechnol, 90,
971-980.
|
|
|
|
|
|
|
M.Widmann,
P.B.Juhl,
and
J.Pleiss
(2010).
Structural classification by the Lipase Engineering Database: a case study of Candida antarctica lipase A.
|
| |
BMC Genomics, 11,
123.
|
|
|
|
|
|
|
S.Rehm,
P.Trodler,
and
J.Pleiss
(2010).
Solvent-induced lid opening in lipases: a molecular dynamics study.
|
| |
Protein Sci, 19,
2122-2130.
|
|
|
|
|
|
|
P.Trodler,
R.D.Schmid,
and
J.Pleiss
(2009).
Modeling of solvent-dependent conformational transitions in Burkholderia cepacia lipase.
|
| |
BMC Struct Biol, 9,
38.
|
|
|
|
|
|
|
D.Brady,
J.Jordaan,
C.Simpson,
A.Chetty,
C.Arumugam,
and
F.S.Moolman
(2008).
Spherezymes: a novel structured self-immobilisation enzyme technology.
|
| |
BMC Biotechnol, 8,
8.
|
|
|
|
|
|
|
J.G.Mala,
and
S.Takeuchi
(2008).
Understanding structural features of microbial lipases-an overview.
|
| |
Anal Chem Insights, 3,
9.
|
|
|
|
|
|
|
M.Moniruzzaman,
M.R.Talukder,
Y.Hayashi,
and
T.Kawanishi
(2007).
Effect of the pretreatment of lipase with organic solvents on its conformation and activity in reverse micelles.
|
| |
Appl Biochem Biotechnol, 142,
253-262.
|
|
|
|
|
|
|
H.A.Wahab,
N.B.Ahmad Khairudin,
M.R.Samian,
and
N.Najimudin
(2006).
Sequence analysis and structure prediction of type II Pseudomonas sp. USM 4-55 PHA synthase and an insight into its catalytic mechanism.
|
| |
BMC Struct Biol, 6,
23.
|
|
|
|
|
|
|
T.W.Xu,
J.H.Xu,
W.Yu,
and
J.H.Zhong
(2006).
Investigating pH and Cu (II) effects on lipase activity and enantioselectivity via kinetic and spectroscopic methods.
|
| |
Biotechnol J, 1,
1293-1301.
|
|
|
|
|
|
|
S.L.Cherukuvada,
A.S.Seshasayee,
K.Raghunathan,
S.Anishetty,
and
G.Pennathur
(2005).
Evidence of a double-lid movement in Pseudomonas aeruginosa lipase: insights from molecular dynamics simulations.
|
| |
PLoS Comput Biol, 1,
e28.
|
|
|
|
|
|
|
S.Y.Chiou,
C.Y.Lai,
L.Y.Lin,
and
G.Lin
(2005).
Probing stereoselective inhibition of the acyl binding site of cholesterol esterase with four diastereomers of 2'-N-alpha-methylbenzylcarbamyl-1, 1'-bi-2-naphthol.
|
| |
BMC Biochem, 6,
17.
|
|
|
|
|
|
|
B.A.Tejo,
A.B.Salleh,
and
J.Pleiss
(2004).
Structure and dynamics of Candida rugosa lipase: the role of organic solvent.
|
| |
J Mol Model, 10,
358-366.
|
|
|
|
|
|
|
C.C.Akoh,
G.C.Lee,
and
J.F.Shaw
(2004).
Protein engineering and applications of Candida rugosa lipase isoforms.
|
| |
Lipids, 39,
513-526.
|
|
|
|
|
|
|
S.H.Chiou,
and
W.T.Wu
(2004).
Immobilization of Candida rugosa lipase on chitosan with activation of the hydroxyl groups.
|
| |
Biomaterials, 25,
197-204.
|
|
|
|
|
|
|
Z.Guo,
and
Y.Sun
(2004).
Characteristics of immobilized lipase on hydrophobic superparamagnetic microspheres to catalyze esterification.
|
| |
Biotechnol Prog, 20,
500-506.
|
|
|
|
|
|
|
G.Fernández-Lorente,
J.M.Palomo,
M.Fuentes,
C.Mateo,
J.M.Guisán,
and
R.Fernández-Lafuente
(2003).
Self-assembly of Pseudomonas fluorescens lipase into bimolecular aggregates dramatically affects functional properties.
|
| |
Biotechnol Bioeng, 82,
232-237.
|
|
|
|
|
|
|
J.M.Mancheño J,
M.A.Pernas M,
M.L.Rúa M,
and
J.A.Hermoso
(2003).
Crystallization and preliminary X-ray diffraction studies of two different crystal forms of the lipase 2 isoform from the yeast Candida rugosa.
|
| |
Acta Crystallogr D Biol Crystallogr, 59,
499-501.
|
|
|
|
|
|
|
S.Brocca,
F.Secundo,
M.Ossola,
L.Alberghina,
G.Carrea,
and
M.Lotti
(2003).
Sequence of the lid affects activity and specificity of Candida rugosa lipase isoenzymes.
|
| |
Protein Sci, 12,
2312-2319.
|
|
|
|
|
|
|
M.Ã.˜.Jensen,
T.R.Jensen,
K.Kjaer,
T.Bjørnholm,
O.G.Mouritsen,
and
G.H.Peters
(2002).
Orientation and conformation of a lipase at an interface studied by molecular dynamics simulations.
|
| |
Biophys J, 83,
98.
|
|
|
|
|
|
|
S.Noinville,
M.Revault,
M.H.Baron,
A.Tiss,
S.Yapoudjian,
M.Ivanova,
and
R.Verger
(2002).
Conformational changes and orientation of Humicola lanuginosa lipase on a solid hydrophobic surface: an in situ interface Fourier transform infrared-attenuated total reflection study.
|
| |
Biophys J, 82,
2709-2719.
|
|
|
|
|
|
|
B.Shenoy,
Y.Wang,
W.Shan,
and
A.L.Margolin
(2001).
Stability of crystalline proteins.
|
| |
Biotechnol Bioeng, 73,
358-369.
|
|
|
|
|
|
|
H.González-Navarro,
M.C.Bañó,
and
C.Abad
(2001).
The closed/open model for lipase activation. Addressing intermediate active forms of fungal enzymes by trapping of conformers in water-restricted environments.
|
| |
Biochemistry, 40,
3174-3183.
|
|
|
|
|
|
|
P.J.O'Connell,
and
J.Varley
(2001).
Immobilization of Candida rugosa lipase on colloidal gas aphrons (CGAs).
|
| |
Biotechnol Bioeng, 74,
264-269.
|
|
|
|
|
|
|
U.H.Kahlow,
R.D.Schmid,
and
J.Pleiss
(2001).
A model of the pressure dependence of the enantioselectivity of Candida rugosalipase towards (+/-)-menthol.
|
| |
Protein Sci, 10,
1942-1952.
|
|
|
|
|
|
|
A.Svendsen
(2000).
Lipase protein engineering.
|
| |
Biochim Biophys Acta, 1543,
223-238.
|
|
|
|
|
|
|
S.Brocca,
M.Persson,
E.Wehtje,
P.Adlercreutz,
L.Alberghina,
and
M.Lotti
(2000).
Mutants provide evidence of the importance of glycosydic chains in the activation of lipase 1 from Candida rugosa.
|
| |
Protein Sci, 9,
985-990.
|
|
|
|
|
|
|
Y.Cajal,
A.Svendsen,
J.De Bolós,
S.A.Patkar,
and
M.A.Alsina
(2000).
Effect of the lipid interface on the catalytic activity and spectroscopic properties of a fungal lipase.
|
| |
Biochimie, 82,
1053-1061.
|
|
|
|
|
|
|
Y.Cajal,
A.Svendsen,
V.Girona,
S.A.Patkar,
and
M.A.Alsina
(2000).
Interfacial control of lid opening in Thermomyces lanuginosa lipase.
|
| |
Biochemistry, 39,
413-423.
|
|
|
|
|
|
|
B.M.Veneziani,
F.Giallauria,
and
F.Gentile
(1999).
The disulfide bond pattern between fragments obtained by the limited proteolysis of bovine thyroglobulin.
|
| |
Biochimie, 81,
517-525.
|
|
|
|
|
|
|
J.G.Oakeshott,
C.Claudianos,
R.J.Russell,
and
G.C.Robin
(1999).
Carboxyl/cholinesterases: a case study of the evolution of a successful multigene family.
|
| |
Bioessays, 21,
1031-1042.
|
|
|
|
|
|
|
J.Zuegg,
K.Gruber,
M.Gugganig,
U.G.Wagner,
and
C.Kratky
(1999).
Three-dimensional structures of enzyme-substrate complexes of the hydroxynitrile lyase from Hevea brasiliensis.
|
| |
Protein Sci, 8,
1990-2000.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
K.E.Jaeger,
B.W.Dijkstra,
and
M.T.Reetz
(1999).
Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases.
|
| |
Annu Rev Microbiol, 53,
315-351.
|
|
|
|
|
|
|
S.Canaan,
A.Roussel,
R.Verger,
and
C.Cambillau
(1999).
Gastric lipase: crystal structure and activity.
|
| |
Biochim Biophys Acta, 1441,
197-204.
|
|
|
|
|
|
|
S.Longhi,
and
C.Cambillau
(1999).
Structure-activity of cutinase, a small lipolytic enzyme.
|
| |
Biochim Biophys Acta, 1441,
185-196.
|
|
|
|
|
|
|
A.Bastida,
P.Sabuquillo,
P.Armisen,
R.Fernandez-Lafuente,
J.Huguet,
and
J.M.Guisan
(1998).
A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports
|
| |
Biotechnol Bioeng, 58,
486-493.
|
|
|
|
|
|
|
D.Mileto,
S.Brocca,
M.Lotti,
M.Takagi,
C.Alquati,
and
L.Alberghina
(1998).
Characterization of the Candida rugosa lipase system and overexpression of the lip1 isoenzyme in a non-conventional yeast.
|
| |
Chem Phys Lipids, 93,
47-55.
|
|
|
|
|
|
|
D.W.Choo,
T.Kurihara,
T.Suzuki,
K.Soda,
and
N.Esaki
(1998).
A cold-adapted lipase of an Alaskan psychrotroph, Pseudomonas sp. strain B11-1: gene cloning and enzyme purification and characterization.
|
| |
Appl Environ Microbiol, 64,
486-491.
|
|
|
|
|
|
|
F.Haeffner,
T.Norin,
and
K.Hult
(1998).
Molecular modeling of the enantioselectivity in lipase-catalyzed transesterification reactions.
|
| |
Biophys J, 74,
1251-1262.
|
|
|
|
|
|
|
J.C.Chen,
L.J.Miercke,
J.Krucinski,
J.R.Starr,
G.Saenz,
X.Wang,
C.A.Spilburg,
L.G.Lange,
J.L.Ellsworth,
and
R.M.Stroud
(1998).
Structure of bovine pancreatic cholesterol esterase at 1.6 A: novel structural features involved in lipase activation.
|
| |
Biochemistry, 37,
5107-5117.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
K.Matsui,
M.Nishioka,
M.Ikeyoshi,
Y.Matsumura,
T.Mori,
and
T.Kajiwara
(1998).
Cucumber root lipoxygenase can act on acyl groups in phosphatidylcholine.
|
| |
Biochim Biophys Acta, 1390,
8.
|
|
|
|
|
|
|
M.Dahim,
and
H.Brockman
(1998).
How colipase-fatty acid interactions mediate adsorption of pancreatic lipase to interfaces.
|
| |
Biochemistry, 37,
8369-8377.
|
|
|
|
|
|
|
S.Brocca,
C.Schmidt-Dannert,
M.Lotti,
L.Alberghina,
and
R.D.Schmid
(1998).
Design, total synthesis, and functional overexpression of the Candida rugosa lip1 gene coding for a major industrial lipase.
|
| |
Protein Sci, 7,
1415-1422.
|
|
|
|
|
|
|
Y.Wei,
L.Swenson,
C.Castro,
U.Derewenda,
W.Minor,
H.Arai,
J.Aoki,
K.Inoue,
L.Servin-Gonzalez,
and
Z.S.Derewenda
(1998).
Structure of a microbial homologue of mammalian platelet-activating factor acetylhydrolases: Streptomyces exfoliatus lipase at 1.9 A resolution.
|
| |
Structure, 6,
511-519.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
A.C.Wallace,
N.Borkakoti,
and
J.M.Thornton
(1997).
TESS: a geometric hashing algorithm for deriving 3D coordinate templates for searching structural databases. Application to enzyme active sites.
|
| |
Protein Sci, 6,
2308-2323.
|
|
|
|
|
|
|
A.V.Efimov
(1997).
Structural trees for protein superfamilies.
|
| |
Proteins, 28,
241-260.
|
|
|
|
|
|
|
C.Löwendahl,
and
S.Allenmark
(1997).
Analysis of a lipase-catalyzed kinetic resolution by chiral normal-phase liquid chromatography.
|
| |
Biomed Chromatogr, 11,
289-295.
|
|
|
|
|
|
|
J.D.Schrag,
Y.Li,
M.Cygler,
D.Lang,
T.Burgdorf,
H.J.Hecht,
R.Schmid,
D.Schomburg,
T.J.Rydel,
J.D.Oliver,
L.C.Strickland,
C.M.Dunaway,
S.B.Larson,
J.Day,
and
A.McPherson
(1997).
The open conformation of a Pseudomonas lipase.
|
| |
Structure, 5,
187-202.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
K.K.Kim,
H.K.Song,
D.H.Shin,
K.Y.Hwang,
and
S.W.Suh
(1997).
The crystal structure of a triacylglycerol lipase from Pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor.
|
| |
Structure, 5,
173-185.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
M.E.Lowe
(1997).
Structure and function of pancreatic lipase and colipase.
|
| |
Annu Rev Nutr, 17,
141-158.
|
|
|
|
|
|
|
S.Longhi,
M.Mannesse,
H.M.Verheij,
G.H.De Haas,
M.Egmond,
E.Knoops-Mouthuy,
and
C.Cambillau
(1997).
Crystal structure of cutinase covalently inhibited by a triglyceride analogue.
|
| |
Protein Sci, 6,
275-286.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
S.R.Feaster,
D.M.Quinn,
and
B.L.Barnett
(1997).
Molecular modeling of the structures of human and rat pancreatic cholesterol esterases.
|
| |
Protein Sci, 6,
73-79.
|
|
|
|
|
|
|
X.Wang,
C.S.Wang,
J.Tang,
F.Dyda,
and
X.C.Zhang
(1997).
The crystal structure of bovine bile salt activated lipase: insights into the bile salt activation mechanism.
|
| |
Structure, 5,
1209-1218.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
Y.Ikushima
(1997).
Supercritical fluids: an interesting medium for chemical and biochemical processes.
|
| |
Adv Colloid Interface Sci, 71,
259-280.
|
|
|
|
|
|
|
A.Kovac,
P.Stadler,
L.Haalck,
F.Spener,
and
F.Paltauf
(1996).
Hydrolysis and esterification of acylglycerols and analogs in aqueous medium catalyzed by microbial lipases.
|
| |
Biochim Biophys Acta, 1301,
57-66.
|
|
|
|
|
|
|
A.Louwrier,
G.J.Drtina,
and
A.M.Klibanov
(1996).
On the issue of interfacial activation of lipase in nonaqueous media.
|
| |
Biotechnol Bioeng, 50,
1-5.
|
|
|
|
|
|
|
A.Nicolas,
M.Egmond,
C.T.Verrips,
J.de Vlieg,
S.Longhi,
C.Cambillau,
and
C.Martinez
(1996).
Contribution of cutinase serine 42 side chain to the stabilization of the oxyanion transition state.
|
| |
Biochemistry, 35,
398-410.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
E.Kynclova,
E.Elsner,
A.Köpf,
G.Hawa,
T.Schalkhammer,
and
F.Pittner
(1996).
Novel method for coupling of poly(ethyleneglycol) to carboxylic acid moieties of proteins.
|
| |
J Mol Recognit, 9,
644-651.
|
|
|
|
|
|
|
G.H.Peters,
D.M.van Aalten,
O.Edholm,
S.Toxvaerd,
and
R.Bywater
(1996).
Dynamics of proteins in different solvent systems: analysis of essential motion in lipases.
|
| |
Biophys J, 71,
2245-2255.
|
|
|
|
|
|
|
J.W.Simons,
H.Adams,
R.C.Cox,
N.Dekker,
F.Götz,
A.J.Slotboom,
and
H.M.Verheij
(1996).
The lipase from Staphylococcus aureus. Expression in Escherichia coli, large-scale purification and comparison of substrate specificity to Staphylococcus hyicus lipase.
|
| |
Eur J Biochem, 242,
760-769.
|
|
|
|
|
|
|
K.Gulomova,
E.Ziomek,
J.D.Schrag,
K.Davranov,
and
M.Cygler
(1996).
Purification and characterization of a Penicillium sp. lipase which discriminates against diglycerides.
|
| |
Lipids, 31,
379-384.
|
|
|
|
|
|
|
M.E.Lowe
(1996).
Mutation of the catalytic site Asp177 to Glu177 in human pancreatic lipase produces an active lipase with increased sensitivity to proteases.
|
| |
Biochim Biophys Acta, 1302,
177-183.
|
|
|
|
|
|
|
M.Holmquist,
F.Haeffner,
T.Norin,
and
K.Hult
(1996).
A structural basis for enantioselective inhibition of Candida rugosa lipase by long-chain aliphatic alcohols.
|
| |
Protein Sci, 5,
83-88.
|
|
|
|
|
|
|
M.J.Brumlik,
and
J.T.Buckley
(1996).
Identification of the catalytic triad of the lipase/acyltransferase from Aeromonas hydrophila.
|
| |
J Bacteriol, 178,
2060-2064.
|
|
|
|
|
|
|
R.Tsuboi,
H.Komatsuzaki,
and
H.Ogawa
(1996).
Induction of an extracellular esterase from Candida albicans and some of its properties.
|
| |
Infect Immun, 64,
2936-2940.
|
|
|
|
|
|
|
S.Jääskeläinen,
X.Y.Wu,
S.Linko,
Y.Wang,
Y.Y.Linko,
O.Teleman,
and
P.Linko
(1996).
Production, characterization, and molecular modeling of lipases for esterification.
|
| |
Ann N Y Acad Sci, 799,
129-138.
|
|
|
|
|
|
|
D.Ghosh,
Z.Wawrzak,
V.Z.Pletnev,
N.Li,
R.Kaiser,
W.Pangborn,
H.Jörnvall,
M.Erman,
and
W.L.Duax
(1995).
Structure of uncomplexed and linoleate-bound Candida cylindracea cholesterol esterase.
|
| |
Structure, 3,
279-288.
|
|
|
PDB code:
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|
G.Zandonella,
L.Haalck,
F.Spener,
K.Faber,
F.Paltauf,
and
A.Hermetter
(1995).
Inversion of lipase stereospecificity for fluorogenic alkyldiacyl glycerols. Effect of substrate solubilization.
|
| |
Eur J Biochem, 231,
50-55.
|
|
|
|
|
|
|
I.Mingarro,
C.Abad,
and
L.Braco
(1995).
Interfacial activation-based molecular bioimprinting of lipolytic enzymes.
|
| |
Proc Natl Acad Sci U S A, 92,
3308-3312.
|
|
|
|
|
|
|
K.M.Loomes
(1995).
Structural organisation of human bile-salt-activated lipase probed by limited proteolysis and expression of a recombinant truncated variant.
|
| |
Eur J Biochem, 230,
607-613.
|
|
|
|
|
|
|
M.A.Swairjo,
N.O.Concha,
M.A.Kaetzel,
J.R.Dedman,
and
B.A.Seaton
(1995).
Ca(2+)-bridging mechanism and phospholipid head group recognition in the membrane-binding protein annexin V.
|
| |
Nat Struct Biol, 2,
968-974.
|
|
|
PDB codes:
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|
|
M.C.Bertolini,
J.D.Schrag,
M.Cygler,
E.Ziomek,
D.Y.Thomas,
and
T.Vernet
(1995).
Expression and characterization of Geotrichum candidum lipase I gene. Comparison of specificity profile with lipase II.
|
| |
Eur J Biochem, 228,
863-869.
|
|
|
|
|
|
|
P.Stadler,
A.Kovac,
L.Haalck,
F.Spener,
and
F.Paltauf
(1995).
Stereoselectivity of microbial lipases. The substitution at position sn-2 of triacylglycerol analogs influences the stereoselectivity of different microbial lipases.
|
| |
Eur J Biochem, 227,
335-343.
|
|
|
|
|
|
|
B.Rubin
(1994).
Grease pit chemistry exposed.
|
| |
Nat Struct Biol, 1,
568-572.
|
|
|
|
|
|
|
D.R.Gjellesvik,
J.B.Lorens,
and
R.Male
(1994).
Pancreatic carboxylester lipase from Atlantic salmon (Salmo salar). cDNA sequence and computer-assisted modelling of tertiary structure.
|
| |
Eur J Biochem, 226,
603-612.
|
|
|
|
|
|
|
J.Uppenberg,
M.T.Hansen,
S.Patkar,
and
T.A.Jones
(1994).
The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica.
|
| |
Structure, 2,
293-308.
|
|
|
PDB codes:
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|
|
K.E.Jaeger,
S.Ransac,
B.W.Dijkstra,
C.Colson,
M.van Heuvel,
and
O.Misset
(1994).
Bacterial lipases.
|
| |
FEMS Microbiol Rev, 15,
29-63.
|
|
|
|
|
|
|
L.Swenson,
R.Green,
R.Joerger,
M.Haas,
K.Scott,
Y.Wei,
U.Derewenda,
D.M.Lawson,
and
Z.S.Derewenda
(1994).
Crystallization and preliminary crystallographic studies of the precursor and mature forms of a neutral lipase from the fungus Rhizopus delemar.
|
| |
Proteins, 18,
301-306.
|
|
|
|
|
|
|
M.C.Bertolini,
L.Laramée,
D.Y.Thomas,
M.Cygler,
J.D.Schrag,
and
T.Vernet
(1994).
Polymorphism in the lipase genes of Geotrichum candidum strains.
|
| |
Eur J Biochem, 219,
119-125.
|
|
|
|
|
|
|
M.Holmquist,
M.Martinelle,
I.G.Clausen,
S.Patkar,
A.Svendsen,
and
K.Hult
(1994).
Trp89 in the lid of Humicola lanuginosa lipase is important for efficient hydrolysis of tributyrin.
|
| |
Lipids, 29,
599-603.
|
|
|
|
|
|
|
M.Norin,
F.Haeffner,
A.Achour,
T.Norin,
and
K.Hult
(1994).
Computer modeling of substrate binding to lipases from Rhizomucor miehei, Humicola lanuginosa, and Candida rugosa.
|
| |
Protein Sci, 3,
1493-1503.
|
|
|
|
|
|
|
N.P.Walker,
R.V.Talanian,
K.D.Brady,
L.C.Dang,
N.J.Bump,
C.R.Ferenz,
S.Franklin,
T.Ghayur,
M.C.Hackett,
and
L.D.Hammill
(1994).
Crystal structure of the cysteine protease interleukin-1 beta-converting enzyme: a (p20/p10)2 homodimer.
|
| |
Cell, 78,
343-352.
|
|
|
|
|
|
|
P.Grochulski,
Y.Li,
J.D.Schrag,
and
M.Cygler
(1994).
Two conformational states of Candida rugosa lipase.
|
| |
Protein Sci, 3,
82-91.
|
|
|
PDB code:
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|
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|
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|
|
R.D.Joerger,
and
M.J.Haas
(1994).
Alteration of chain length selectivity of a Rhizopus delemar lipase through site-directed mutagenesis.
|
| |
Lipids, 29,
377-384.
|
|
|
|
|
|
|
R.J.Kazlauskas
(1994).
Elucidating structure-mechanism relationships in lipases: prospects for predicting and engineering catalytic properties.
|
| |
Trends Biotechnol, 12,
464-472.
|
|
|
|
|
|
|
S.Santamarina-Fojo,
and
H.B.Brewer
(1994).
Lipoprotein lipase: structure, function and mechanism of action.
|
| |
Int J Clin Lab Res, 24,
143-147.
|
|
|
|
|
|
|
U.Derewenda,
L.Swenson,
R.Green,
Y.Wei,
G.G.Dodson,
S.Yamaguchi,
M.J.Haas,
and
Z.S.Derewenda
(1994).
An unusual buried polar cluster in a family of fungal lipases.
|
| |
Nat Struct Biol, 1,
36-47.
|
|
|
PDB code:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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|
Links
