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Chemokine PDB id
1cm9
Jmol
Contents
Protein chains
68 a.a. *
Waters ×22
* Residue conservation analysis
PDB id:
1cm9
Name: Chemokine
Title: Crystal structure of viral macrophage inflammatory protein-i
Structure: Protein (viral macrophage inflammatory protein-ii chain: a, b. Synonym: vmip-ii. Other_details: synthetic
Source: Human herpesvirus 8. Organism_taxid: 37296. Other_details: non-biological sequence
Biol. unit: Dimer (from PQS)
Resolution:
2.10Å     R-factor:   0.240     R-free:   0.275
Authors: E.J.Fernandez,E.Lolis
Key ref:
E.J.Fernandez et al. (2000). Comparison of the structure of vMIP-II with eotaxin-1, RANTES, and MCP-3 suggests a unique mechanism for CCR3 activation. Biochemistry, 39, 12837-12844. PubMed id: 11041848 DOI: 10.1021/bi001166f
Date:
19-May-99     Release date:   24-Jun-99    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q98157  (VMI2_HHV8P) -  Viral macrophage inflammatory protein 2
Seq:
Struc: 		94 a.a.
68 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   2 terms 
  Biological process     immune response   1 term 
  Biochemical function     cytokine activity     2 terms  

 

 
DOI no: 10.1021/bi001166f Biochemistry 39:12837-12844 (2000)
PubMed id: 11041848  
 
 
Comparison of the structure of vMIP-II with eotaxin-1, RANTES, and MCP-3 suggests a unique mechanism for CCR3 activation.
E.J.Fernandez, J.Wilken, D.A.Thompson, S.C.Peiper, E.Lolis.
 
  ABSTRACT  
 
Herpesvirus-8 macrophage inflammatory protein-II (vMIP-II) binds a uniquely wide spectrum of chemokine receptors. We report the X-ray structure of vMIP-II determined to 2.1 A resolution. Like RANTES, vMIP-II crystallizes as a dimer and displays the conventional chemokine tertiary fold. We have compared the surface topology and electrostatic potential of vMIP-II to those of eotaxin-1, RANTES, and MCP-3, three CCR3 physiological agonists with known three-dimensional structures. Surface epitopes identified on RANTES to be involved in binding to CCR3 are mimicked on the eotaxin-1 and MCP-3 surface. However, the surface topology of vMIP-II in these regions is markedly different. The results presented here indicate that the structural basis for interaction with the chemokine receptor CCR3 by vMIP-II is different from that for the physiological agonists eotaxin-1, RANTES, and MCP-3. These differences on vMIP-II may be a consequence of its broad-range receptor recognition capabilities.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20077567 J.W.Murphy, H.Yuan, Y.Kong, Y.Xiong, and E.J.Lolis (2010).
Heterologous quaternary structure of CXCL12 and its relationship to the CC chemokine family.
  Proteins, 78, 1331-1337.
PDB codes: 3gv3 3hp3
18059478 G.V.Crichlow, H.Zhou, H.H.Hsiao, K.B.Frederick, M.Debrosse, Y.Yang, E.J.Folta-Stogniew, H.J.Chung, C.Fan, E.M.De la Cruz, D.Levens, E.Lolis, and D.Braddock (2008).
Dimerization of FIR upon FUSE DNA binding suggests a mechanism of c-myc inhibition.
  EMBO J, 27, 277-289.
PDB code: 2qfj
17686848 D.Liu, N.Madani, Y.Li, R.Cao, W.T.Choi, S.P.Kawatkar, M.Y.Lim, S.Kumar, C.Z.Dong, J.Wang, J.D.Russell, C.R.Lefebure, J.An, S.Wilson, Y.G.Gao, L.A.Pallansch, J.G.Sodroski, and Z.Huang (2007).
Crystal structure and structural mechanism of a novel anti-human immunodeficiency virus and D-amino acid-containing chemokine.
  J Virol, 81, 11489-11498.  
17433398 M.Miller-Kittrell, J.Sai, M.Penfold, A.Richmond, and T.E.Sparer (2007).
Functional characterization of chimpanzee cytomegalovirus chemokine, vCXCL-1(CCMV).
  Virology, 364, 454-465.  
17243149 Y.Li, D.Liu, R.Cao, S.Kumar, C.Dong, J.An, S.R.Wilson, Y.G.Gao, and Z.Huang (2007).
Crystal structure of chemically synthesized vMIP-II.
  Proteins, 67, 243-246.
PDB codes: 2fht 2fj2
15931460 A.Jeshtadi, G.Henriquet, S.M.Laidlaw, D.Hot, Y.Zhang, and M.A.Skinner (2005).
In vitro expression and analysis of secreted fowlpox virus CC chemokine-like proteins Fpv060, Fpv061, Fpv116 and Fpv121.
  Arch Virol, 150, 1745-1762.  
15039444 V.Petkovic, C.Moghini, S.Paoletti, M.Uguccioni, and B.Gerber (2004).
Eotaxin-3/CCL26 is a natural antagonist for CC chemokine receptors 1 and 5. A human chemokine with a regulatory role.
  J Biol Chem, 279, 23357-23363.  
11807180 E.J.Fernandez, and E.Lolis (2002).
Structure, function, and inhibition of chemokines.
  Annu Rev Pharmacol Toxicol, 42, 469-499.  
11745373 M.Houimel, P.Loetscher, M.Baggiolini, and L.Mazzucchelli (2001).
Functional inhibition of CCR3-dependent responses by peptides derived from phage libraries.
  Eur J Immunol, 31, 3535-3545.  
11551942 N.Zhou, Z.Luo, J.Luo, D.Liu, J.W.Hall, R.J.Pomerantz, and Z.Huang (2001).
Structural and functional characterization of human CXCR4 as a chemokine receptor and HIV-1 co-receptor by mutagenesis and molecular modeling studies.
  J Biol Chem, 276, 42826-42833.  
11737246 T.Rozmyslowicz, J.Kijowski, D.O.Conover, M.Majka, M.Baj-Krzyworzeka, R.Reca, J.J.Libura, G.N.Gaulton, and M.Z.Ratajczak (2001).
New T-lymphocytic cell lines for studying cell infectability by human immunodeficiency virus.
  Eur J Haematol, 67, 142-151.  
11358512 W.Shao, E.Fernandez, A.Sachpatzidis, J.Wilken, D.A.Thompson, B.I.Schweitzer, and E.Lolis (2001).
CCR2 and CCR5 receptor-binding properties of herpesvirus-8 vMIP-II based on sequence analysis and its solution structure.
  Eur J Biochem, 268, 2948-2959.
PDB code: 1hhv
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.