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Immune system PDB id
1cl7
Jmol
Contents
Protein chains
216 a.a. *
135 a.a. *
82 a.a. *
* Residue conservation analysis
PDB id:
1cl7
Name: Immune system
Title: Anti hiv1 protease fab
Structure: Protein (igg1 antibody 1696 (light chain)). Chain: l. Fragment: fab. Synonym: immunoglobulin, igg1. Protein (igg1 antibody 1696 (variable heavy chain)). Chain: h. Fragment: fab. Synonym: immunoglobulin, igg1.
Source: Mus musculus. House mouse. Organism_taxid: 10090. Strain: balb/c. Cell_line: hybridoma. Cell_line: hybridoma
Biol. unit: Trimer (from PQS)
Resolution:
3.00Å     R-factor:   0.183     R-free:   0.280
Authors: J.Lescar,G.A.Bentley
Key ref: J.Lescar et al. (1999). Inhibition of the HIV-1 and HIV-2 proteases by a monoclonal antibody. Protein Sci, 8, 2686-2696. PubMed id: 10631984 Ref: Full text
Date:
06-May-99     Release date:   12-Jan-00    
PROCHECK
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 Headers
 References

Protein chain
No UniProt id for this chain
Protein chain
No UniProt id for this chain
Protein chain
Pfam   ArchSchema ?
P01869  (IGH1M_MOUSE) -  Ig gamma-1 chain C region, membrane-bound form
Seq:
Struc: 		393 a.a.
82 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
Full text Protein Sci 8:2686-2696 (1999)
PubMed id: 10631984  
 
 
Inhibition of the HIV-1 and HIV-2 proteases by a monoclonal antibody.
J.Lescar, J.Brynda, P.Rezacova, R.Stouracova, M.M.Riottot, V.Chitarra, M.Fabry, M.Horejsi, J.Sedlacek, G.A.Bentley.
 
  ABSTRACT  
 
The monoclonal antibody 1696, directed against the HIV-1 protease, displays strong inhibitory effects toward the catalytic activity of the enzyme of both the HIV-1 and HIV-2 isolates. This antibody cross-reacts with peptides that include the N-terminus of the enzyme, a region that is well conserved in sequence among different viral strains and which, furthermore, is crucial for homodimerization to the active enzymatic form. This observation, as well as antigen-binding studies in the presence of an active site inhibitor, suggest that 1696 inhibits the HIV protease by destabilizing its active homodimeric form. To characterize further how the antibody 1696 inhibits the HIV-1 and HIV-2 proteases, we have solved the crystal structure of its Fab fragment by molecular replacement and refined it at 3.0 A resolution. The antigen binding site has a deep cavity at its center, which is lined mainly by acidic and hydrophobic residues, and is large enough to accommodate several antigen residues. The structure of the Fab 1696 could form a starting basis for the design of alternative HIV protease-inhibiting molecules of broad specificity.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19264660 R.Rajamanonmani, C.Nkenfou, P.Clancy, Y.H.Yau, S.G.Shochat, S.Sukupolvi-Petty, W.Schul, M.S.Diamond, S.G.Vasudevan, and J.Lescar (2009).
On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes.
  J Gen Virol, 90, 799-809.  
12777823 J.Lescar, J.Brynda, M.Fabry, M.Horejsi, P.Rezacova, J.Sedlacek, and G.A.Bentley (2003).
Structure of a single-chain Fv fragment of an antibody that inhibits the HIV-1 and HIV-2 proteases.
  Acta Crystallogr D Biol Crystallogr, 59, 955-957.
PDB code: 1n4x
12447903 P.Rezacova, J.Brynda, M.Fabry, M.Horejsi, R.Stouracova, J.Lescar, V.Chitarra, M.M.Riottot, J.Sedlacek, and G.A.Bentley (2002).
Inhibition of HIV protease by monoclonal antibodies.
  J Mol Recognit, 15, 272-276.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.