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PDBsum entry 1ckk

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protein metals Protein-protein interface(s) links
Calmodulin-peptide complex PDB id
1ckk
Jmol
Contents
Protein chains
148 a.a. *
26 a.a. *
Metals
_CA ×4
* Residue conservation analysis
PDB id:
1ckk
Name: Calmodulin-peptide complex
Title: Calmodulin/rat ca2+/calmodulin dependent protein kinase fragment
Structure: Protein (calmodulin). Chain: a. Synonym: cam. Engineered: yes. Protein (rat ca2+/calmodulin dependent protein kinase). Chain: b. Fragment: calmodulin binding domain. Synonym: camkk.
Source: Xenopus laevis. African clawed frog. Organism_taxid: 8355. Gene: xenopus laevis. Expressed in: escherichia coli. Expression_system_taxid: 562. Rattus norvegicus. Norway rat. Organism_taxid: 10116
NMR struc: 30 models
Authors: M.Osawa,H.Tokumitsu,M.B.Swindells,H.Kurihara,M.Orita, T.Shibanuma,T.Furuya,M.Ikura
Key ref:
M.Osawa et al. (1999). A novel target recognition revealed by calmodulin in complex with Ca2+-calmodulin-dependent kinase kinase. Nat Struct Biol, 6, 819-824. PubMed id: 10467092 DOI: 10.1038/12271
Date:
20-Nov-98     Release date:   10-Sep-99    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P62155  (CALM_XENLA) -  Calmodulin
Seq:
Struc:
149 a.a.
148 a.a.
Protein chain
Pfam   ArchSchema ?
P97756  (KKCC1_RAT) -  Calcium/calmodulin-dependent protein kinase kinase 1
Seq:
Struc:
505 a.a.
26 a.a.
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chain B: E.C.2.7.11.17  - Calcium/calmodulin-dependent protein kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + a protein = ADP + a phosphoprotein
ATP
+ protein
= ADP
+ phosphoprotein
      Cofactor: Ca(2+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biochemical function     protein binding     4 terms  

 

 
    reference    
 
 
DOI no: 10.1038/12271 Nat Struct Biol 6:819-824 (1999)
PubMed id: 10467092  
 
 
A novel target recognition revealed by calmodulin in complex with Ca2+-calmodulin-dependent kinase kinase.
M.Osawa, H.Tokumitsu, M.B.Swindells, H.Kurihara, M.Orita, T.Shibanuma, T.Furuya, M.Ikura.
 
  ABSTRACT  
 
The structure of calcium-bound calmodulin (Ca2+/CaM) complexed with a 26-residue peptide, corresponding to the CaM-binding domain of rat Ca2+/CaM-dependent protein kinase kinase (CaMKK), has been determined by NMR spectroscopy. In this complex, the CaMKK peptide forms a fold comprising an alpha-helix and a hairpin-like loop whose C-terminus folds back on itself. The binding orientation of this CaMKK peptide by the two CaM domains is opposite to that observed in all other CaM-target complexes determined so far. The N- and C-terminal hydrophobic pockets of Ca2+/CaM anchor Trp 444 and Phe 459 of the CaMKK peptide, respectively. This 14-residue separation between two key hydrophobic groups is also unique among previously determined CaM complexes. The present structure represents a new and distinct class of Ca2+/CaM target recognition that may be shared by other Ca2+/CaM-stimulated proteins.
 
  Selected figure(s)  
 
Figure 2.
Figure 2. a, Schematic drawing of interacting residues between Ca ^2+ /CaM and CaMKK peptide. Residues in N-domain and C-domain are colored in cyan and violet, respectively. Key residues of the CaMKK peptide anchoring the hydrophobic pocket in each domain, Trp 444 and Phe 459, are shown in green. b, Portions of ^13 C/F[3]-filtered ^13 C/F[1]-edited HMQC-NOESY spectrum ^24 showing intermolecular NOEs between CaM and the CaMKK peptide. Stereo drawing of the key residues of CaMKK peptide in the hydrophobic pocket of c, N-domain and d,C−domain. esidues within 5 Å of the key residues, Trp 444 and Phe 459, are shown. N, O and S atoms are colored in blue, red and yellow, respectively, while C atoms of N- and C-domain of CaM and CaMKK are shown in cyan, violet and gray, respectively. Diagrams (c) and (d) were generated using the program MOLMOL^55.
Figure 3.
Figure 3. Electrostatic potential surfaces of the CaM−target peptide complexes. In the upper panels, the surface of Ca ^2+ /CaM is shown with the target peptide as an yellow tube. The peptide surface is in the lower panels. The surface is colored according to the local electrostatic potential, with blue and red representing positive and negative potential, respectively. Acidic and basic residues interacting with the target peptide are labeled in red and blue, respectively. a, CaM−CaMKK; b, CaM−MLCK^19, ^20; c, CaM−CaMKII^21. The domain linker of CaM in the CaM−CaMKII complex was modeled in the Insight II, since residues 74−83 are absent from the PDB file because of the high flexibility of this region.
 
  The above figures are reprinted by permission from Macmillan Publishers Ltd: Nat Struct Biol (1999, 6, 819-824) copyright 1999.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21360154 J.L.Gifford, H.Ishida, and H.J.Vogel (2011).
Fast methionine-based solution structure determination of calcium-calmodulin complexes.
  J Biomol NMR, 50, 71-81.
PDB code: 2l7l
20653564 H.Tokumitsu, N.Hatano, M.Tsuchiya, S.Yurimoto, T.Fujimoto, N.Ohara, R.Kobayashi, and H.Sakagami (2010).
Identification and characterization of PRG-1 as a neuronal calmodulin-binding protein.
  Biochem J, 431, 81-91.  
20383153 M.S.Marlow, J.Dogan, K.K.Frederick, K.G.Valentine, and A.J.Wand (2010).
The role of conformational entropy in molecular recognition by calmodulin.
  Nat Chem Biol, 6, 352-358.  
19996092 N.Juranic, E.Atanasova, A.G.Filoteo, S.Macura, F.G.Prendergast, J.T.Penniston, and E.E.Strehler (2010).
Calmodulin wraps around its binding domain in the plasma membrane Ca2+ pump anchored by a novel 18-1 motif.
  J Biol Chem, 285, 4015-4024.
PDB code: 2kne
20459766 N.Uchikoga, and T.Hirokawa (2010).
Analysis of protein-protein docking decoys using interaction fingerprints: application to the reconstruction of CaM-ligand complexes.
  BMC Bioinformatics, 11, 236.  
19998355 Y.Zhang, H.Tan, G.Chen, and Z.Jia (2010).
Investigating the disorder-order transition of calmodulin binding domain upon binding calmodulin using molecular dynamics simulation.
  J Mol Recognit, 23, 360-368.  
19197237 C.Neufeld, F.V.Filipp, B.Simon, A.Neuhaus, N.Schüller, C.David, H.Kooshapur, T.Madl, R.Erdmann, W.Schliebs, M.Wilmanns, and M.Sattler (2009).
Structural basis for competitive interactions of Pex14 with the import receptors Pex5 and Pex19.
  EMBO J, 28, 745-754.
PDB codes: 2w84 2w85
19305019 D.A.Macdougall, S.Wachten, A.Ciruela, A.Sinz, and D.M.Cooper (2009).
Separate elements within a single IQ-like motif in adenylyl cyclase type 8 impart ca2+/calmodulin binding and autoinhibition.
  J Biol Chem, 284, 15573-15588.  
19667195 J.P.Junker, and M.Rief (2009).
Single-molecule force spectroscopy distinguishes target binding modes of calmodulin.
  Proc Natl Acad Sci U S A, 106, 14361-14366.  
19214217 L.Wang, K.Tsuda, M.Sato, J.D.Cohen, F.Katagiri, and J.Glazebrook (2009).
Arabidopsis CaM binding protein CBP60g contributes to MAMP-induced SA accumulation and is involved in disease resistance against Pseudomonas syringae.
  PLoS Pathog, 5, e1000301.  
19452629 N.T.Wright, B.R.Cannon, P.T.Wilder, M.T.Morgan, K.M.Varney, D.B.Zimmer, and D.J.Weber (2009).
Solution structure of S100A1 bound to the CapZ peptide (TRTK12).
  J Mol Biol, 386, 1265-1277.  
19106096 Q.K.Kleerekoper, and J.A.Putkey (2009).
PEP-19, an Intrinsically Disordered Regulator of Calmodulin Signaling.
  J Biol Chem, 284, 7455-7464.  
19583261 S.J.Abraham, R.P.Nolet, R.J.Calvert, L.M.Anderson, and V.Gaponenko (2009).
The hypervariable region of K-Ras4B is responsible for its specific interactions with calmodulin.
  Biochemistry, 48, 7575-7583.  
18089570 A.Scheschonka, S.Findlow, R.Schemm, O.El Far, J.H.Caldwell, M.P.Crump, K.Holden-Dye, V.O'Connor, H.Betz, and J.M.Werner (2008).
Structural determinants of calmodulin binding to the intracellular C-terminal domain of the metabotropic glutamate receptor 7A.
  J Biol Chem, 283, 5577-5588.  
18326633 D.S.Libich, and G.Harauz (2008).
Backbone dynamics of the 18.5 kDa isoform of myelin basic protein reveals transient alpha-helices and a calmodulin-binding site.
  Biophys J, 94, 4847-4866.  
18940602 E.Y.Kim, C.H.Rumpf, Y.Fujiwara, E.S.Cooley, F.Van Petegem, and D.L.Minor (2008).
Structures of CaV2 Ca2+/CaM-IQ domain complexes reveal binding modes that underlie calcium-dependent inactivation and facilitation.
  Structure, 16, 1455-1467.
PDB codes: 3dve 3dvj 3dvk 3dvm
18326489 L.He, Z.Hou, and R.Z.Qi (2008).
Calmodulin binding and Cdk5 phosphorylation of p35 regulate its effect on microtubules.
  J Biol Chem, 283, 13252-13260.  
18400181 M.X.Mori, C.W.Vander Kooi, D.J.Leahy, and D.T.Yue (2008).
Crystal structure of the CaV2 IQ domain in complex with Ca2+/calmodulin: high-resolution mechanistic implications for channel regulation by Ca2+.
  Structure, 16, 607-620.  
18518982 N.V.Valeyev, D.G.Bates, P.Heslop-Harrison, I.Postlethwaite, and N.V.Kotov (2008).
Elucidating the mechanisms of cooperative calcium-calmodulin interactions: a structural systems biology approach.
  BMC Syst Biol, 2, 48.  
18583346 Q.Guo, J.E.Jureller, J.T.Warren, E.Solomaha, J.Florián, and W.J.Tang (2008).
Protein-protein docking and analysis reveal that two homologous bacterial adenylyl cyclase toxins interact with calmodulin differently.
  J Biol Chem, 283, 23836-23845.  
18384083 Q.Ye, H.Wang, J.Zheng, Q.Wei, and Z.Jia (2008).
The complex structure of calmodulin bound to a calcineurin peptide.
  Proteins, 73, 19-27.
PDB code: 2r28
18461636 Y.Zhang, H.Tan, Z.Jia, and G.Chen (2008).
Ligand-induced dimer formation of calmodulin.
  J Mol Recognit, 21, 267-274.  
17502097 E.Pham, J.Chiang, I.Li, W.Shum, and K.Truong (2007).
A computational tool for designing FRET protein biosensors by rigid-body sampling of their conformational space.
  Structure, 15, 515-523.  
17947243 H.S.Kim, B.O.Park, J.H.Yoo, M.S.Jung, S.M.Lee, H.J.Han, K.E.Kim, S.H.Kim, C.O.Lim, D.J.Yun, S.Y.Lee, and W.S.Chung (2007).
Identification of a calmodulin-binding NAC protein as a transcriptional repressor in Arabidopsis.
  J Biol Chem, 282, 36292-36302.  
17377988 J.R.Horton, S.J.Elgar, S.I.Khan, X.Zhang, P.A.Wade, and X.Cheng (2007).
Structure of the SANT domain from the Xenopus chromatin remodeling factor ISWI.
  Proteins, 67, 1198-1202.
PDB code: 2nog
17552906 S.L.Russell, N.V.McFerran, E.M.Hoey, A.Trudgett, and D.J.Timson (2007).
Characterisation of two calmodulin-like proteins from the liver fluke, Fasciola hepatica.
  Biol Chem, 388, 593-599.  
17901047 Y.Zhou, W.Yang, M.M.Lurtz, Y.Ye, Y.Huang, H.W.Lee, Y.Chen, C.F.Louis, and J.J.Yang (2007).
Identification of the calmodulin binding domain of connexin 43.
  J Biol Chem, 282, 35005-35017.  
17027503 A.A.Maximciuc, J.A.Putkey, Y.Shamoo, and K.R.Mackenzie (2006).
Complex of calmodulin with a ryanodine receptor target reveals a novel, flexible binding mode.
  Structure, 14, 1547-1556.
PDB code: 2bcx
16568447 A.Ganoth, E.Nachliel, R.Friedman, and M.Gutman (2006).
Molecular dynamics study of a calmodulin-like protein with an IQ peptide: spontaneous refolding of the protein around the peptide.
  Proteins, 64, 133-146.  
16844751 A.Ganoth, R.Friedman, E.Nachliel, and M.Gutman (2006).
A molecular dynamics study and free energy analysis of complexes between the Mlc1p protein and two IQ motif peptides.
  Biophys J, 91, 2436-2450.  
17151196 A.Houdusse, J.F.Gaucher, E.Krementsova, S.Mui, K.M.Trybus, and C.Cohen (2006).
Crystal structure of apo-calmodulin bound to the first two IQ motifs of myosin V reveals essential recognition features.
  Proc Natl Acad Sci U S A, 103, 19326-19331.
PDB code: 2ix7
16464867 A.Raichaudhuri, R.Bhattacharyya, S.Chaudhuri, P.Chakrabarti, and M.Dasgupta (2006).
Domain analysis of a groundnut calcium-dependent protein kinase: nuclear localization sequence in the junction domain is coupled with nonconsensus calcium binding domains.
  J Biol Chem, 281, 10399-10409.
PDB code: 2g3u
16623711 D.E.Spratt, E.Newman, J.Mosher, D.K.Ghosh, J.C.Salerno, and J.G.Guillemette (2006).
Binding and activation of nitric oxide synthase isozymes by calmodulin EF hand pairs.
  FEBS J, 273, 1759-1771.  
16956364 J.Martinez-Sanz, A.Yang, Y.Blouquit, P.Duchambon, L.Assairi, and C.T.Craescu (2006).
Binding of human centrin 2 to the centrosomal protein hSfi1.
  FEBS J, 273, 4504-4515.  
16721661 K.Chen, J.Ruan, and L.A.Kurgan (2006).
Prediction of three dimensional structure of calmodulin.
  Protein J, 25, 57-70.  
16267044 L.Baekgaard, L.Luoni, M.I.De Michelis, and M.G.Palmgren (2006).
The plant plasma membrane Ca2+ pump ACA8 contains overlapping as well as physically separated autoinhibitory and calmodulin-binding domains.
  J Biol Chem, 281, 1058-1065.  
16432210 M.Ikura, and J.B.Ames (2006).
Genetic polymorphism and protein conformational plasticity in the calmodulin superfamily: two ways to promote multifunctionality.
  Proc Natl Acad Sci U S A, 103, 1159-1164.  
16846216 M.S.Marlow, and A.J.Wand (2006).
Conformational dynamics of calmodulin in complex with the calmodulin-dependent kinase kinase alpha calmodulin-binding domain.
  Biochemistry, 45, 8732-8741.  
16493654 P.Radivojac, S.Vucetic, T.R.O'Connor, V.N.Uversky, Z.Obradovic, and A.K.Dunker (2006).
Calmodulin signaling: analysis and prediction of a disorder-dependent molecular recognition.
  Proteins, 63, 398-410.  
16785321 S.Li, A.M.Sandercock, P.Conduit, C.V.Robinson, R.L.Williams, and J.V.Kilmartin (2006).
Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication.
  J Cell Biol, 173, 867-877.
PDB codes: 2doq 2gv5
  16511158 C.L.Chyan, P.C.Huang, T.H.Lin, J.W.Huang, S.S.Lin, H.B.Huang, and Y.C.Chen (2005).
Purification, crystallization and preliminary crystallographic studies of a calmodulin-OLFp hybrid molecule.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 61, 785-787.  
16299511 F.Van Petegem, F.C.Chatelain, and D.L.Minor (2005).
Insights into voltage-gated calcium channel regulation from the structure of the CaV1.2 IQ domain-Ca2+/calmodulin complex.
  Nat Struct Mol Biol, 12, 1108-1115.
PDB code: 2be6
16193483 G.Fiorin, R.R.Biekofsky, A.Pastore, and P.Carloni (2005).
Unwinding the helical linker of calcium-loaded calmodulin: a molecular dynamics study.
  Proteins, 61, 829-839.  
15803393 G.M.Contessa, M.Orsale, S.Melino, V.Torre, M.Paci, A.Desideri, and D.O.Cicero (2005).
Structure of calmodulin complexed with an olfactory CNG channel fragment and role of the central linker: residual dipolar couplings to evaluate calmodulin binding modes outside the kinase family.
  J Biomol NMR, 31, 185-199.
PDB code: 1sy9
16338416 J.L.Fallon, D.B.Halling, S.L.Hamilton, and F.A.Quiocho (2005).
Structure of calmodulin bound to the hydrophobic IQ domain of the cardiac Ca(v)1.2 calcium channel.
  Structure, 13, 1881-1886.
PDB codes: 2f3y 2f3z
15583004 L.Xiong, Q.K.Kleerekoper, R.He, J.A.Putkey, and S.L.Hamilton (2005).
Sites on calmodulin that interact with the C-terminal tail of Cav1.2 channel.
  J Biol Chem, 280, 7070-7079.  
14670974 A.P.Yamniuk, and H.J.Vogel (2004).
Structurally homologous binding of plant calmodulin isoforms to the calmodulin-binding domain of vacuolar calcium-ATPase.
  J Biol Chem, 279, 7698-7707.  
15213382 C.H.Yun, J.Bai, D.Y.Sun, D.F.Cui, W.R.Chang, and D.C.Liang (2004).
Structure of potato calmodulin PCM6: the first report of the three-dimensional structure of a plant calmodulin.
  Acta Crystallogr D Biol Crystallogr, 60, 1214-1219.
PDB code: 1rfj
15138276 E.Newman, D.E.Spratt, J.Mosher, B.Cheyne, H.J.Montgomery, D.L.Wilson, J.B.Weinberg, S.M.Smith, J.C.Salerno, D.K.Ghosh, and J.G.Guillemette (2004).
Differential activation of nitric-oxide synthase isozymes by calmodulin-troponin C chimeras.
  J Biol Chem, 279, 33547-33557.  
15100408 I.Bertini, C.Del Bianco, I.Gelis, N.Katsaros, C.Luchinat, G.Parigi, M.Peana, A.Provenzani, and M.A.Zoroddu (2004).
Experimentally exploring the conformational space sampled by domain reorientation in calmodulin.
  Proc Natl Acad Sci U S A, 101, 6841-6846.
PDB code: 1sw8
14570888 J.H.Yoo, M.S.Cheong, C.Y.Park, B.C.Moon, M.C.Kim, Y.H.Kang, H.C.Park, M.S.Choi, J.H.Lee, W.Y.Jung, H.W.Yoon, W.S.Chung, C.O.Lim, S.Y.Lee, and M.J.Cho (2004).
Regulation of the dual specificity protein phosphatase, DsPTP1, through interactions with calmodulin.
  J Biol Chem, 279, 848-858.  
15130477 M.A.Schumacher, M.Crum, and M.C.Miller (2004).
Crystal structures of apocalmodulin and an apocalmodulin/SK potassium channel gating domain complex.
  Structure, 12, 849-860.
PDB codes: 1qx5 1qx7
14765114 M.Matsubara, T.Nakatsu, H.Kato, and H.Taniguchi (2004).
Crystal structure of a myristoylated CAP-23/NAP-22 N-terminal domain complexed with Ca2+/calmodulin.
  EMBO J, 23, 712-718.
PDB code: 1l7z
12538886 A.M.Weljie, A.P.Yamniuk, H.Yoshino, Y.Izumi, and H.J.Vogel (2003).
Protein conformational changes studied by diffusion NMR spectroscopy: application to helix-loop-helix calcium binding proteins.
  Protein Sci, 12, 228-236.  
12890685 A.Popescu, S.Miron, Y.Blouquit, P.Duchambon, P.Christova, and C.T.Craescu (2003).
Xeroderma pigmentosum group C protein possesses a high affinity binding site to human centrin 2 and calmodulin.
  J Biol Chem, 278, 40252-40261.  
12824496 D.S.Libich, C.M.Hill, I.R.Bates, F.R.Hallett, S.Armstrong, A.Siemiarczuk, and G.Harauz (2003).
Interaction of the 18.5-kD isoform of myelin basic protein with Ca2+ -calmodulin: effects of deimination assessed by intrinsic Trp fluorescence spectroscopy, dynamic light scattering, and circular dichroism.
  Protein Sci, 12, 1507-1521.  
12577052 E.Yamauchi, T.Nakatsu, M.Matsubara, H.Kato, and H.Taniguchi (2003).
Crystal structure of a MARCKS peptide containing the calmodulin-binding domain in complex with Ca2+-calmodulin.
  Nat Struct Biol, 10, 226-231.
PDB code: 1iwq
12540834 H.Tokumitsu, H.Inuzuka, Y.Ishikawa, and R.Kobayashi (2003).
A single amino acid difference between alpha and beta Ca2+/calmodulin-dependent protein kinase kinase dictates sensitivity to the specific inhibitor, STO-609.
  J Biol Chem, 278, 10908-10913.  
12626519 I.Berger, C.Bieniossek, C.Schaffitzel, M.Hassler, E.Santelli, and T.J.Richmond (2003).
Direct interaction of Ca2+/calmodulin inhibits histone deacetylase 5 repressor core binding to myocyte enhancer factor 2.
  J Biol Chem, 278, 17625-17635.  
14597710 J.M.Shifman, and S.L.Mayo (2003).
Exploring the origins of binding specificity through the computational redesign of calmodulin.
  Proc Natl Acad Sci U S A, 100, 13274-13279.  
12574113 M.Aoyagi, A.S.Arvai, J.A.Tainer, and E.D.Getzoff (2003).
Structural basis for endothelial nitric oxide synthase binding to calmodulin.
  EMBO J, 22, 766-775.
PDB code: 1niw
12542690 S.W.Vetter, and E.Leclerc (2003).
Novel aspects of calmodulin target recognition and activation.
  Eur J Biochem, 270, 404-414.  
  12234092 D.S.Libich, and G.Harauz (2002).
Interactions of the 18.5-kDa isoform of myelin basic protein with Ca(2+)-calmodulin: in vitro studies using fluorescence microscopy and spectroscopy.
  Biochem Cell Biol, 80, 395-406.  
11867640 H.Tokumitsu, H.Inuzuka, Y.Ishikawa, M.Ikeda, I.Saji, and R.Kobayashi (2002).
STO-609, a specific inhibitor of the Ca(2+)/calmodulin-dependent protein kinase kinase.
  J Biol Chem, 277, 15813-15818.  
12455978 J.D.Joseph, and A.R.Means (2002).
Calcium binding is required for calmodulin function in Aspergillus nidulans.
  Eukaryot Cell, 1, 119-125.  
11904288 J.K.Kranz, E.K.Lee, A.C.Nairn, and A.J.Wand (2002).
A direct test of the reductionist approach to structural studies of calmodulin activity: relevance of peptide models of target proteins.
  J Biol Chem, 277, 16351-16354.  
11904292 M.C.Kim, S.H.Lee, J.K.Kim, H.J.Chun, M.S.Choi, W.S.Chung, B.C.Moon, C.H.Kang, C.Y.Park, J.H.Yoo, Y.H.Kang, S.C.Koo, Y.D.Koo, J.C.Jung, S.T.Kim, P.Schulze-Lefert, S.Y.Lee, and M.J.Cho (2002).
Mlo, a modulator of plant defense and cell death, is a novel calmodulin-binding protein. Isolation and characterization of a rice Mlo homologue.
  J Biol Chem, 277, 19304-19314.  
12111723 M.Ikura, M.Osawa, and J.B.Ames (2002).
The role of calcium-binding proteins in the control of transcription: structure to function.
  Bioessays, 24, 625-636.  
12124297 M.L.Mattinen, K.Pääkkönen, T.Ikonen, J.Craven, T.Drakenberg, R.Serimaa, J.Waltho, and A.Annila (2002).
Quaternary structure built from subunits combining NMR and small-angle x-ray scattering data.
  Biophys J, 83, 1177-1183.  
11847276 N.Hayashi, M.Matsubara, Y.Jinbo, K.Titani, Y.Izumi, and N.Matsushima (2002).
Nef of HIV-1 interacts directly with calcium-bound calmodulin.
  Protein Sci, 11, 529-537.  
11723128 R.Wissmann, W.Bildl, H.Neumann, A.F.Rivard, N.Klöcker, D.Weitz, U.Schulte, J.P.Adelman, D.Bentrop, and B.Fakler (2002).
A helical region in the C terminus of small-conductance Ca2+-activated K+ channels controls assembly with apo-calmodulin.
  J Biol Chem, 277, 4558-4564.
PDB code: 1kkd
12441389 S.R.Martin, and P.M.Bayley (2002).
Regulatory implications of a novel mode of interaction of calmodulin with a double IQ-motif target sequence from murine dilute myosin V.
  Protein Sci, 11, 2909-2923.  
12023286 W.T.Barry, C.Boudignon-Proudhon, D.D.Shock, A.McFadden, J.M.Weiss, J.Sondek, and L.V.Parise (2002).
Molecular basis of CIB binding to the integrin alpha IIb cytoplasmic domain.
  J Biol Chem, 277, 28877-28883.  
11170410 B.R.Sorensen, J.T.Eppel, and M.A.Shea (2001).
Paramecium calmodulin mutants defective in ion channel regulation associate with melittin in the absence of calcium but require it for tertiary collapse.
  Biochemistry, 40, 896-903.  
11266605 G.Larsson, J.Schleucher, J.Onions, S.Hermann, T.Grundström, and S.S.Wijmenga (2001).
A novel target recognition revealed by calmodulin in complex with the basic helix--loop--helix transcription factor SEF2-1/E2-2.
  Protein Sci, 10, 169-186.  
11702071 K.Truong, A.Sawano, H.Mizuno, H.Hama, K.I.Tong, T.K.Mal, A.Miyawaki, and M.Ikura (2001).
FRET-based in vivo Ca2+ imaging by a new calmodulin-GFP fusion molecule.
  Nat Struct Biol, 8, 1069-1073.  
11114499 A.Lewit-Bentley, and S.Réty (2000).
EF-hand calcium-binding proteins.
  Curr Opin Struct Biol, 10, 637-643.  
10792048 A.Ulrich, A.A.Schmitz, T.Braun, T.Yuan, H.J.Vogel, and G.Vergères (2000).
Mapping the interface between calmodulin and MARCKS-related protein by fluorescence spectroscopy.
  Proc Natl Acad Sci U S A, 97, 5191-5196.  
10884684 D.Chin, and A.R.Means (2000).
Calmodulin: a prototypical calcium sensor.
  Trends Cell Biol, 10, 322-328.  
11076504 H.Ishida, K.Takahashi, K.Nakashima, Y.Kumaki, M.Nakata, K.Hikichi, and M.Yazawa (2000).
Solution structures of the N-terminal domain of yeast calmodulin: Ca2+-dependent conformational change and its functional implication.
  Biochemistry, 39, 13660-13668.
PDB codes: 1f54 1f55
10841769 O.R.Jaren, S.Harmon, A.F.Chen, and M.A.Shea (2000).
Paramecium calmodulin mutants defective in ion channel regulation can bind calcium and undergo calcium-induced conformational switching.
  Biochemistry, 39, 6881-6890.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.