PDBsum entry 1c5g

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protein links
Blood clotting PDB id
Protein chain
379 a.a. *
* Residue conservation analysis
PDB id:
Name: Blood clotting
Title: Plasminogen activator inhibitor-1
Structure: Plasminogen activator inhibitor-1. Chain: a. Synonym: pai-1. Engineered: yes. Other_details: latent form
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562.
2.60Å     R-factor:   not given    
Authors: E.J.Goldsmith
Key ref: H.M.Tucker et al. (1995). Engineering of plasminogen activator inhibitor-1 to reduce the rate of latency transition. Nat Struct Biol, 2, 442-445. PubMed id: 7664104
07-Dec-99     Release date:   17-Dec-99    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
P05121  (PAI1_HUMAN) -  Plasminogen activator inhibitor 1
402 a.a.
379 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular space   1 term 


Nat Struct Biol 2:442-445 (1995)
PubMed id: 7664104  
Engineering of plasminogen activator inhibitor-1 to reduce the rate of latency transition.
H.M.Tucker, J.Mottonen, E.J.Goldsmith, R.D.Gerard.


Literature references that cite this PDB file's key reference

  PubMed id Reference
21280127 L.C.Thompson, S.Goswami, D.S.Ginsberg, D.E.Day, I.M.Verhamme, and C.B.Peterson (2011).
Metals affect the structure and activity of human plasminogen activator inhibitor-1. I. Modulation of stability and protease inhibition.
  Protein Sci, 20, 353-365.  
17400919 J.Y.Yi, and H.Im (2007).
Structural factors affecting the choice between latency transition and polymerization in inhibitory serpins.
  Protein Sci, 16, 833-841.  
15576554 Y.R.Na, and H.Im (2005).
The length of the reactive center loop modulates the latency transition of plasminogen activator inhibitor-1.
  Protein Sci, 14, 55-63.  
14963029 Z.Xu, R.D.Balsara, N.V.Gorlatova, D.A.Lawrence, F.J.Castellino, and V.A.Ploplis (2004).
Conservation of critical functional domains in murine plasminogen activator inhibitor-1.
  J Biol Chem, 279, 17914-17920.  
12694181 T.Wind, J.K.Jensen, D.M.Dupont, P.Kulig, and P.A.Andreasen (2003).
Mutational analysis of plasminogen activator inhibitor-1.
  Eur J Biochem, 270, 1680-1688.  
12244055 H.Im, M.S.Woo, K.Y.Hwang, and M.H.Yu (2002).
Interactions causing the kinetic trap in serpin protein folding.
  J Biol Chem, 277, 46347-46354.
PDB code: 1iz2
11928815 T.Wind, M.Hansen, J.K.Jensen, and P.A.Andreasen (2002).
The molecular basis for anti-proteolytic and non-proteolytic functions of plasminogen activator inhibitor type-1: roles of the reactive centre loop, the shutter region, the flexible joint region and the small serpin fragment.
  Biol Chem, 383, 21-36.  
11555638 D.N.Saunders, L.Jankova, S.J.Harrop, P.M.Curmi, A.R.Gould, M.Ranson, and M.S.Baker (2001).
Interaction between the P14 residue and strand 2 of beta-sheet B is critical for reactive center loop insertion in plasminogen activator inhibitor-2.
  J Biol Chem, 276, 43383-43389.  
11714919 S.P.Bottomley, I.D.Lawrenson, D.Tew, W.Dai, J.C.Whisstock, and R.N.Pike (2001).
The role of strand 1 of the C beta-sheet in the structure and function of alpha(1)-antitrypsin.
  Protein Sci, 10, 2518-2524.  
11685246 S.Ye, A.L.Cech, R.Belmares, R.C.Bergstrom, Y.Tong, D.R.Corey, M.R.Kanost, and E.J.Goldsmith (2001).
The structure of a Michaelis serpin-protease complex.
  Nat Struct Biol, 8, 979-983.
PDB codes: 1i99 1k9o
10848991 M.Fa, F.Bergström, J.Karolin, L.B.Johansson, and T.Ny (2000).
Conformational studies of plasminogen activator inhibitor type 1 by fluorescence spectroscopy. Analysis of the reactive centre of inhibitory and substrate forms, and of their respective reactive-centre cleaved forms.
  Eur J Biochem, 267, 3729-3734.  
10913251 T.J.Stout, H.Graham, D.I.Buckley, and D.J.Matthews (2000).
Structures of active and latent PAI-1: a possible stabilizing role for chloride ions.
  Biochemistry, 39, 8460-8469.
PDB codes: 1dvm 1dvn
10368279 A.M.Sharp, P.E.Stein, N.S.Pannu, R.W.Carrell, M.B.Berkenpas, D.Ginsburg, D.A.Lawrence, and R.J.Read (1999).
The active conformation of plasminogen activator inhibitor 1, a target for drugs to control fibrinolysis and cell adhesion.
  Structure, 7, 111-118.
PDB code: 1b3k
9521649 C.M.Lukacs, H.Rubin, and D.W.Christianson (1998).
Engineering an anion-binding cavity in antichymotrypsin modulates the "spring-loaded" serpin-protease interaction.
  Biochemistry, 37, 3297-3304.
PDB codes: 1as4 3caa 4caa
9477948 P.Björquist, J.Ehnebom, T.Inghardt, L.Hansson, M.Lindberg, M.Linschoten, M.Strömqvist, and J.Deinum (1998).
Identification of the binding site for a low-molecular-weight inhibitor of plasminogen activator inhibitor type 1 by site-directed mutagenesis.
  Biochemistry, 37, 1227-1234.  
9452500 W.S.Chang, and D.A.Lomas (1998).
Latent alpha1-antichymotrypsin. A molecular explanation for the inactivation of alpha1-antichymotrypsin in chronic bronchitis and emphysema.
  J Biol Chem, 273, 3695-3701.  
9634700 Y.Xue, P.Björquist, T.Inghardt, M.Linschoten, D.Musil, L.Sjölin, and J.Deinum (1998).
Interfering with the inhibitory mechanism of serpins: crystal structure of a complex formed between cleaved plasminogen activator inhibitor type 1 and a reactive-centre loop peptide.
  Structure, 6, 627-636.
PDB code: 1a7c
9335576 M.R.Wardell, W.S.Chang, D.Bruce, R.Skinner, A.M.Lesk, and R.W.Carrell (1997).
Preparative induction and characterization of L-antithrombin: a structural homologue of latent plasminogen activator inhibitor-1.
  Biochemistry, 36, 13133-13142.  
9241254 S.H.Ke, and E.L.Madison (1997).
Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method.
  Nucleic Acids Res, 25, 3371-3372.  
9252355 S.H.Ke, G.S.Coombs, K.Tachias, D.R.Corey, and E.L.Madison (1997).
Optimal subsite occupancy and design of a selective inhibitor of urokinase.
  J Biol Chem, 272, 20456-20462.  
8652525 A.Gils, I.Knockaert, and P.J.Declerck (1996).
Substrate behavior of plasminogen activator inhibitor-1 is not associated with a lack of insertion of the reactive site loop.
  Biochemistry, 35, 7474-7481.  
8836107 C.M.Lukacs, J.Q.Zhong, M.I.Plotnick, H.Rubin, B.S.Cooperman, and D.W.Christianson (1996).
Arginine substitutions in the hinge region of antichymotrypsin affect serpin beta-sheet rearrangement.
  Nat Struct Biol, 3, 888-893.
PDB codes: 1ct3 2caa
8662688 G.Deng, G.Royle, S.Wang, K.Crain, and D.J.Loskutoff (1996).
Structural and functional analysis of the plasminogen activator inhibitor-1 binding motif in the somatomedin B domain of vitronectin.
  J Biol Chem, 271, 12716-12723.  
8787534 H.T.Wright (1996).
The structural puzzle of how serpin serine proteinase inhibitors work.
  Bioessays, 18, 453-464.  
8987976 Z.Wang, J.Mottonen, and E.J.Goldsmith (1996).
Kinetically controlled folding of the serpin plasminogen activator inhibitor 1.
  Biochemistry, 35, 16443-16448.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.