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Complex (humanized antibody/hydrolase)
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PDB id
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1bvk
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Contents |
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108 a.a.
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116 a.a.
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129 a.a.
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* Residue conservation analysis
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PDB id:
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| Name: |
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Complex (humanized antibody/hydrolase)
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Title:
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Humanized anti-lysozyme fv complexed with lysozyme
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Structure:
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Hulys11. Chain: a, d. Fragment: fv. Engineered: yes. Hulys11. Chain: b, e. Fragment: fv. Engineered: yes. Lysozyme.
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Gallus gallus. Chicken. Organism_taxid: 9031. Other_details: egg white
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Biol. unit:
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Trimer (from
)
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Resolution:
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2.70Å
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R-factor:
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0.208
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R-free:
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0.297
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Authors:
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M.A.Holmes,T.N.Buss,J.Foote
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Key ref:
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M.A.Holmes
et al.
(1998).
Conformational correction mechanisms aiding antigen recognition by a humanized antibody.
J Exp Med,
187,
479-485.
PubMed id:
DOI:
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Date:
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16-Sep-98
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Release date:
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16-Feb-99
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PROCHECK
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Headers
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References
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No UniProt id for this chain
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Enzyme class:
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Chains C, F:
E.C.3.2.1.17
- Lysozyme.
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Reaction:
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Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.
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Gene Ontology (GO) functional annotation
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Cellular component
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extracellular region
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2 terms
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Biological process
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metabolic process
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4 terms
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Biochemical function
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catalytic activity
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5 terms
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DOI no:
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J Exp Med
187:479-485
(1998)
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PubMed id:
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Conformational correction mechanisms aiding antigen recognition by a humanized antibody.
|
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M.A.Holmes,
T.N.Buss,
J.Foote.
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ABSTRACT
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The crystal structure of the complex between hen egg lysozyme and the Fv
fragment of a humanized antilysozyme antibody was determined to 2.7-A
resolution. The structure of the antigen combining site in the complex is nearly
identical to that of the complexed form of the parent mouse antibody, D1.3. In
contrast, the combining sites of the unliganded mouse and humanized
antilysozymes show moderate conformational differences. This disparity suggests
that a conformational readjustment process linked to antigen binding reverses
adverse conformations in the complementarity determining regions that had been
introduced by engineering these segments next to human framework regions in the
humanized antibody.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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G.Malviya,
F.Conti,
M.Chianelli,
F.Scopinaro,
R.A.Dierckx,
and
A.Signore
(2010).
Molecular imaging of rheumatoid arthritis by radiolabelled monoclonal antibodies: new imaging strategies to guide molecular therapies.
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Eur J Nucl Med Mol Imaging, 37,
386-398.
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T.Nakanishi,
K.Tsumoto,
A.Yokota,
H.Kondo,
and
I.Kumagai
(2008).
Critical contribution of VH-VL interaction to reshaping of an antibody: the case of humanization of anti-lysozyme antibody, HyHEL-10.
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Protein Sci, 17,
261-270.
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PDB codes:
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D.Segal,
and
M.Eisenstein
(2005).
The effect of resolution-dependent global shape modifications on rigid-body protein-protein docking.
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Proteins, 59,
580-591.
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A.Berchanski,
B.Shapira,
and
M.Eisenstein
(2004).
Hydrophobic complementarity in protein-protein docking.
|
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Proteins, 56,
130-142.
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H.Haruyama,
S.Ito,
K.Miyadai,
T.Takahashi,
R.Kawaida,
T.Takayama,
H.Hanzawa,
T.Hata,
J.Yamaguchi,
H.Yoshida-Kato,
K.Ichikawa,
J.Ohsumi,
S.Yonehara,
and
N.Serizawa
(2002).
Humanization of the mouse anti-Fas antibody HFE7A and crystal structure of the humanized HFE7A Fab fragment.
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Biol Pharm Bull, 25,
1537-1545.
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PDB code:
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R.B.Fong,
Z.Ding,
A.S.Hoffman,
and
P.S.Stayton
(2002).
Affinity separation using an Fv antibody fragment-"smart" polymer conjugate.
|
| |
Biotechnol Bioeng, 79,
271-276.
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C.Rader
(2001).
Antibody libraries in drug and target discovery.
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Drug Discov Today, 6,
36-43.
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W.Dall'Acqua,
and
P.Carter
(1998).
Antibody engineering.
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| |
Curr Opin Struct Biol, 8,
443-450.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
