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PDBsum entry 1bts
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Transmembrane protein PDB id
1bts

 

 

 

 

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Contents
Protein chain
23 a.a.
PDB id:
1bts
Name: Transmembrane protein
Title: The solution structures of the first and second transmembrane-spanning segments of band 3
Structure: Band 3 anion transport protein. Chain: a. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606
NMR struc: 1 models
Authors: A.R.Gargaro,G.B.Bloomberg,C.E.Dempsey,M.Murray,M.J.A.Tanner
Key ref: A.R.Gargaro et al. (1994). The solution structures of the first and second transmembrane-spanning segments of band 3. Eur J Biochem, 221, 445-454. PubMed id: 8168533
Date:
03-Aug-94     Release date:   01-Nov-94    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P02730  (B3AT_HUMAN) -  Band 3 anion transport protein from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		911 a.a.
22 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 1 residue position (black cross)

 

 
Eur J Biochem 221:445-454 (1994)
PubMed id: 8168533  
 
 
The solution structures of the first and second transmembrane-spanning segments of band 3.
A.R.Gargaro, G.B.Bloomberg, C.E.Dempsey, M.Murray, M.J.Tanner.
 
  ABSTRACT  
 
We have studied the structures of synthetic peptides which correspond to the proposed first and second membrane-spanning segments of the human red cell anion transporter (band 3). The peptides, which were acetylated at their N-termini and amidated at the C-termini, comprise the 20 amino acids of residues 405-424 and 21 amino acids of residues 436-456 of the human band 3 sequence. The solution structures of the peptides in trifluoroethanol were studied by two-dimensional NMR spectroscopy. Characteristic NOEs were observed indicating that the peptides adopted a predominantly alpha-helical structure in trifluoroethanol solution. Dynamical simulated annealing using the program XPLOR was employed for the structure calculations. The amide exchange rates in trifluoroethanol have also been measured and are consistent with an alpha-helical structure for the peptides.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21455267 T.Hirai, N.Hamasaki, T.Yamaguchi, and Y.Ikeda (2011).
Topology models of anion exchanger 1 that incorporate the anti-parallel V-shaped motifs found in the EM structure.
  Biochem Cell Biol, 89, 148-156.  
  19177358 R.C.Page, S.Lee, J.D.Moore, S.J.Opella, and T.A.Cross (2009).
Backbone structure of a small helical integral membrane protein: A unique structural characterization.
  Protein Sci, 18, 134-146.
PDB code: 2k3m
17215879 J.K.Rainey, L.Fliegel, and B.D.Sykes (2006).
Strategies for dealing with conformational sampling in structural calculations of flexible or kinked transmembrane peptides.
  Biochem Cell Biol, 84, 918-929.  
15946193 B.S.Lou, T.H.Lin, and C.Z.Lo (2005).
The interactions of phenytoin and its binding site in DI-S6 segment of Na+ channel voltage-gated peptide by NMR spectroscopy and molecular modeling study.
  J Pept Res, 66, 27-38.  
12044163 G.Choi, J.Landin, J.F.Galan, R.R.Birge, A.D.Albert, and P.L.Yeagle (2002).
Structural studies of metarhodopsin II, the activated form of the G-protein coupled receptor, rhodopsin.
  Biochemistry, 41, 7318-7324.
PDB code: 1ln6
11876646 H.Kuma, A.A.Shinde, T.R.Howren, and M.L.Jennings (2002).
Topology of the anion exchange protein AE1: the controversial sidedness of lysine 743.
  Biochemistry, 41, 3380-3388.  
10895257 L.J.Bruce, and M.J.Tanner (1999).
Erythroid band 3 variants and disease.
  Baillieres Best Pract Res Clin Haematol, 12, 637-654.  
10213635 V.Wray, R.Kinder, T.Federau, P.Henklein, B.Bechinger, and U.Schubert (1999).
Solution structure and orientation of the transmembrane anchor domain of the HIV-1-encoded virus protein U by high-resolution and solid-state NMR spectroscopy.
  Biochemistry, 38, 5272-5282.  
9709005 D.Askin, G.B.Bloomberg, E.J.Chambers, and M.J.Tanner (1998).
NMR solution structure of a cytoplasmic surface loop of the human red cell anion transporter, band 3.
  Biochemistry, 37, 11670-11678.
PDB code: 1bh7
  9491367 M.J.Tanner (1997).
The structure and function of band 3 (AE1): recent developments (review).
  Mol Membr Biol, 14, 155-165.  
7632880 C.M.Deber, and S.C.Li (1995).
Peptides in membranes: helicity and hydrophobicity.
  Biopolymers, 37, 295-318.  
8528765 R.A.Reithmeier (1995).
Characterization and modeling of membrane proteins using sequence analysis.
  Curr Opin Struct Biol, 5, 491-500.  
7994570 L.J.Smith, A.T.Alexandrescu, M.Pitkeathly, and C.M.Dobson (1994).
Solution structure of a peptide fragment of human alpha-lactalbumin in trifluoroethanol: a model for local structure in the molten globule.
  Structure, 2, 703-712.  
7986536 R.A.Reithmeier (1994).
Mammalian exchangers and co-transporters.
  Curr Opin Cell Biol, 6, 583-594.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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