PDBsum entry: 1bqh

Immune system

PDB-id

1bqh


	Asymmetric unit

	Main view

Contents

Description

Header details

Protein chains

122 a.a. *

Ligands

ARG-GLY-TYR-VAL-
TYR-GLN-GLY-LEU ×2

NDG

NAG ×3

* Residue conservation analysis

Tools

Image Generation

AstexViewer™@PDBe

Run PROCHECK

Clefts Calculation

		Biological unit, pentamer (as deduced by PQS)

PDB id:

1bqh

Name:

Immune system

Title:

Murine cd8aa ectodomain fragment in complex with h-2kb/vsv8

Structure:

Protein (h-2 class i histocompatibility antigen). Chain: a, d. Fragment: alpha chain. Protein (beta-2-microglobulin ). Chain: b, e. Fragment: beta chain. Protein (vsv8). Chain: c, f. Fragment: antigenic peptide.

Source:

Mus musculus. House mouse. Organism_taxid: 10090. Synthetic: yes. Expressed in: mus musculus. Expression_system_taxid: 10090. Expression_system_cell_line: cho lec 3.2.8.1. Other_details: gene fused to thrombin cleavage site and leucine zipper

Biological unit:

Pentamer (from PQS)

UniProt:

Chains A, D: P01901 (HA1B_MOUSE)

Seq:

Struc:


Seq:				369 a.a.

Struc:				274 a.a.

Chains B, E: P01887 (B2MG_MOUSE)

Seq:				119 a.a.

Struc:				99 a.a.

Chains G, H, I, K: P01731 (CD8A_MOUSE)

Seq:

247 a.a.

Struc:

122 a.a.

Key:		PfamA domain
		Secondary structure			CATH domain

Resolution:

2.80Å

R-factor:

0.212

R-free:

0.296

Authors:

J.H.Wang,E.L.Reinherz,P.S.Kern,H.C.Chang

Key ref:

P.S.Kern et al. (1998). Structural basis of CD8 coreceptor function revealed by crystallographic analysis of a murine CD8alphaalpha ectodomain fragment in complex with H-2Kb.. Immunity, 9, 519-530. [PubMed id: 9806638] [DOI: 10.1016/S1074-7613(00)80635-4]

Date:

16-Aug-98

Release date:

19-Aug-98

Quick_links

RCSB

PDBe

SRS

MMDB

JenaLib

OCA

PDBWiki

Proteopedia

CATH

SCOP

FSSP

HSSP

PDBSWS

PQS

ProSAT

Whatcheck

EDS

Procheck

Surface

Key reference

DOI no: 10.1016/S1074-7613(00)80635-4 Immunity 9:519-530 (1998)

PubMed id: 9806638

Structural basis of CD8 coreceptor function revealed by crystallographic analysis of a murine CD8alphaalpha ectodomain fragment in complex with H-2Kb.

P.S.Kern, M.K.Teng, A.Smolyar, J.H.Liu, J.Liu, R.E.Hussey, R.Spoerl, H.C.Chang, E.L.Reinherz, J.H.Wang.

ABSTRACT

The crystal structure of the two immunoglobulin variable-like domains of the murine CD8alphaalpha homodimer complexed to the class I MHC H-2Kb molecule at 2.8 A resolution shows that CD8alphaalpha binds to the protruding MHC alpha3 domain loop in an antibody-like manner. Comparison of mouse CD8alphaalpha/H-2Kb and human CD8alphaalpha/HLA-A2 complexes reveals shared as well as species-specific recognition features. In both species, coreceptor function apparently involves the participation of CD8 dimer in a bidentate attachment to an MHC class I molecule in conjunction with a T cell receptor without discernable conformational alteration of the peptide or MHC antigen-presenting platform.

Selected figure(s)

Figure 1.
Figure 1. Overall View of the m-CD8aa/ VSV8-H-2K b Crystal Structure Figure 5.
Figure 5. Detailed Interaction among mCD8aa and H-2K b

The above figures are reprinted by permission from Cell Press: Immunity (1998, 9, 519-530) copyright 1998.

Figures were selected by the author.

Literature references that cite this PDB file's key reference

PubMed id Reference

19565478 J.D.Taurog, M.L.Dorris, N.Satumtira, T.M.Tran, R.Sharma, R.Dressel, J.van den Brandt, and H.M.Reichardt (2009).
Spondylarthritis in HLA-B27/human beta(2)-microglobulin-transgenic rats is not prevented by lack of CD8.

Arthritis Rheum, 60, 1977-1984.

19137599 L.Zong, Y.Chen, H.Peng, F.Gao, A.Iwamoto, and G.F.Gao (2009).
Rhesus macaque: a tight homodimeric CD8alphaalpha.

Proteins, 75, 241-244.

19472182 Y.Chen, Y.Shi, H.Cheng, Y.Q.An, and G.F.Gao (2009).
Structural immunology and crystallography help immunologists see the immune system in action: how T and NK cells touch their ligands.

IUBMB Life, 61, 579-590.

18726714 E.J.Collins, and D.S.Riddle (2008).
TCR-MHC docking orientation: natural selection, or thymic selection?

Immunol Res, 41, 267-294.

18650389 G.J.Freeman (2008).
Structures of PD-1 with its ligands: sideways and dancing cheek to cheek.

Proc Natl Acad Sci U S A, 105, 10275-10276.

18703505 T.Mareeva, E.Martinez-Hackert, and Y.Sykulev (2008).
How a T cell receptor-like antibody recognizes major histocompatibility complex-bound peptide.

J Biol Chem, 283, 29053-29059.

PDB codes: 3cvh 3cvi

17369261 D.Hatherley, K.Harlos, D.C.Dunlop, D.I.Stuart, and A.N.Barclay (2007).
The structure of the macrophage signal regulatory protein alpha (SIRPalpha) inhibitory receptor reveals a binding face reminiscent of that used by T cell receptors.

J Biol Chem, 282, 14567-14575.

PDB code: 2uv3

17438291 P.D.Holler, T.Yamagata, W.Jiang, M.Feuerer, C.Benoist, and D.Mathis (2007).
The same genomic region conditions clonal deletion and clonal deviation to the CD8alphaalpha and regulatory T cell lineages in NOD versus C57BL/6 mice.

Proc Natl Acad Sci U S A, 104, 7187-7192.

16918695 B.Rubin, J.Riond, J.Leghait, and J.E.Gairin (2006).
Interactions between CD8alphabeta and the TCRalphabeta/CD3-receptor complex.

Scand J Immunol, 64, 260-270.

16840780 H.C.Chang, K.Tan, and Y.M.Hsu (2006).
CD8alphabeta has two distinct binding modes of interaction with peptide-major histocompatibility complex class I.

J Biol Chem, 281, 28090-28096.

16500675 K.Natarajan, A.Hicks, J.Mans, H.Robinson, R.Guan, R.A.Mariuzza, and D.H.Margulies (2006).
Crystal structure of the murine cytomegalovirus MHC-I homolog m144.

J Mol Biol, 358, 157-171.

PDB code: 1u58

16551255 M.G.Rudolph, R.L.Stanfield, and I.A.Wilson (2006).
How TCRs bind MHCs, peptides, and coreceptors.

Annu Rev Immunol, 24, 419-466.

15614759 F.Glaser, Y.Rosenberg, A.Kessel, T.Pupko, and N.Ben-Tal (2005).
The ConSurf-HSSP database: the mapping of evolutionary conservation among homologs onto PDB structures.

Proteins, 58, 610-617.

15837791 L.Wooldridge, H.A.van den Berg, M.Glick, E.Gostick, B.Laugel, S.L.Hutchinson, A.Milicic, J.M.Brenchley, D.C.Douek, D.A.Price, and A.K.Sewell (2005).
Interaction between the CD8 coreceptor and major histocompatibility complex class I stabilizes T cell receptor-antigen complexes at the cell surface.

J Biol Chem, 280, 27491-27501.

15805102 M.Cebecauer, P.Guillaume, S.Mark, O.Michielin, N.Boucheron, M.Bezard, B.H.Meyer, J.M.Segura, H.Vogel, and I.F.Luescher (2005).
CD8+ cytotoxic T lymphocyte activation by soluble major histocompatibility complex-peptide dimers.

J Biol Chem, 280, 23820-23828.

16089503 R.Olson, K.E.Huey-Tubman, C.Dulac, and P.J.Bjorkman (2005).
Structure of a pheromone receptor-associated MHC molecule with an open and empty groove.

PLoS Biol, 3, e257.

PDB code: 1zs8

15660246 W.Held, A.Chalifour, and J.D.Coudert (2005).
Regulation of natural killer cell function: a role for the NK cell's own MHC class I molecules.

Med Microbiol Immunol, 194, 169-174.

16231286 W.Zhong, and E.L.Reinherz (2005).
CD8 alpha alpha homodimer expression and role in CD8 T cell memory generation during influenza virus A infection in mice.

Eur J Immunol, 35, 3103-3110.

14555655 R.C.Hillig, M.Hülsmeyer, W.Saenger, K.Welfle, R.Misselwitz, H.Welfle, C.Kozerski, A.Volz, B.Uchanska-Ziegler, and A.Ziegler (2004).
Thermodynamic and structural analysis of peptide- and allele-dependent properties of two HLA-B27 subtypes exhibiting differential disease association.

J Biol Chem, 279, 652-663.

PDB code: 1jgd

12676960 A.H.Merry, R.J.Gilbert, D.A.Shore, L.Royle, O.Miroshnychenko, M.Vuong, M.R.Wormald, D.J.Harvey, R.A.Dwek, B.J.Classon, P.M.Rudd, and S.J.Davis (2003).
O-glycan sialylation and the structure of the stalk-like region of the T cell co-receptor CD8.

J Biol Chem, 278, 27119-27128.

12459555 A.M.Moody, S.J.North, B.Reinhold, S.J.Van Dyken, M.E.Rogers, M.Panico, A.Dell, H.R.Morris, J.D.Marth, and E.L.Reinherz (2003).
Sialic acid capping of CD8beta core 1-O-glycans controls thymocyte-major histocompatibility complex class I interaction.

J Biol Chem, 278, 7240-7246.

12615890 P.A.van der Merwe, and S.J.Davis (2003).
Molecular interactions mediating T cell antigen recognition.

Annu Rev Immunol, 21, 659-684.

11861620 K.Natarajan, N.Dimasi, J.Wang, R.A.Mariuzza, and D.H.Margulies (2002).
Structure and function of natural killer cell receptors: multiple molecular solutions to self, nonself discrimination.

Annu Rev Immunol, 20, 853-885.

11988465 M.G.Rudolph, J.G.Luz, and I.A.Wilson (2002).
Structural and thermodynamic correlates of T cell signaling.

Annu Rev Biophys Biomol Struct, 31, 121-149.

11536178 A.M.Moody, Y.Xiong, H.C.Chang, and E.L.Reinherz (2001).
The CD8alphabeta co-receptor on double-positive thymocytes binds with differing affinities to the products of distinct class I MHC loci.

Eur J Immunol, 31, 2791-2799.

11535811 J.H.Wang, R.Meijers, Y.Xiong, J.H.Liu, T.Sakihama, R.Zhang, A.Joachimiak, and E.L.Reinherz (2001).
Crystal structure of the human CD4 N-terminal two-domain fragment complexed to a class II MHC molecule.

Proc Natl Acad Sci U S A, 98, 10799-10804.

PDB code: 1jl4

11148219 N.Matsumoto, M.Mitsuki, K.Tajima, W.M.Yokoyama, and K.Yamamoto (2001).
The functional binding site for the C-type lectin-like natural killer cell receptor Ly49A spans three domains of its major histocompatibility complex class I ligand.

J Exp Med, 193, 147-158.

11579290 S.Jurcevic, P.Chandler, S.H.Sacks, and E.Simpson (2001).
Rapid rejection of HLA-A2 transgenic skin graft due to indirect allorecognition.

Transplantation, 72, 994-997.

11606747 T.A.Potter, K.Grebe, B.Freiberg, and A.Kupfer (2001).
Formation of supramolecular activation clusters on fresh ex vivo CD8+ T cells after engagement of the T cell antigen receptor and CD8 by antigen-presenting cells.

Proc Natl Acad Sci U S A, 98, 12624-12629.

10634787 C.Wingren, M.P.Crowley, M.Degano, Y.Chien, and I.A.Wilson (2000).
Crystal structure of a gammadelta T cell receptor ligand T22: a truncated MHC-like fold.

Science, 287, 310-314.

PDB code: 1c16

10809759 G.F.Gao, B.E.Willcox, J.R.Wyer, J.M.Boulter, C.A.O'Callaghan, K.Maenaka, D.I.Stuart, E.Y.Jones, P.A.Van Der Merwe, J.I.Bell, and B.K.Jakobsen (2000).
Classical and nonclassical class I major histocompatibility complex molecules exhibit subtle conformational differences that affect binding to CD8alphaalpha.

J Biol Chem, 275, 15232-15238.

11045629 O.Schueler-Furman, Y.Altuvia, A.Sette, and H.Margalit (2000).
Structure-based prediction of binding peptides to MHC class I molecules: application to a broad range of MHC alleles.

Protein Sci, 9, 1838-1846.

10377186 F.Tilloy, E.Treiner, S.H.Park, C.Garcia, F.Lemonnier, H.de la Salle, A.Bendelac, M.Bonneville, and O.Lantz (1999).
An invariant T cell receptor alpha chain defines a novel TAP-independent major histocompatibility complex class Ib-restricted alpha/beta T cell subpopulation in mammals.

J Exp Med, 189, 1907-1921.

10631938 K.C.Garcia (1999).
Molecular interactions between extracellular components of the T-cell receptor signaling complex.

Immunol Rev, 172, 73-85.

10493174 L.Devine, and P.B.Kavathas (1999).
Molecular analysis of protein interactions mediating the function of the cell surface protein CD8.

Immunol Res, 19, 201-210.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.