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Signaling protein PDB id
1bo0
Jmol
Contents
Protein chain
76 a.a. *
* Residue conservation analysis
PDB id:
1bo0
Name: Signaling protein
Title: Monocyte chemoattractant protein-3, nmr, minimized average structure
Structure: Protein (monocyte chemoattractant protein-3). Chain: a. Synonym: mcp-3. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
NMR struc: 1 models
Authors: D.Kwon,D.Lee,B.D.Sykes,K.-S.Kim
Key ref:
K.S.Kim et al. (1996). Structural characterization of a monomeric chemokine: monocyte chemoattractant protein-3. FEBS Lett, 395, 277-282. PubMed id: 8898111 DOI: 10.1016/0014-5793(96)01024-1
Date:
10-Aug-98     Release date:   10-Oct-99    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P80098  (CCL7_HUMAN) -  C-C motif chemokine 7
Seq:
Struc: 		99 a.a.
76 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   2 terms 
  Biological process     immune response   6 terms 
  Biochemical function     cytokine activity     4 terms  

 

 
DOI no: 10.1016/0014-5793(96)01024-1 FEBS Lett 395:277-282 (1996)
PubMed id: 8898111  
 
 
Structural characterization of a monomeric chemokine: monocyte chemoattractant protein-3.
K.S.Kim, K.Rajarathnam, I.Clark-Lewis, B.D.Sykes.
 
  ABSTRACT  
 
1H-NMR spectroscopy and analytical ultracentrifugation studies reveal that monocyte chemoattractant protein-3 (MCP-3) is a monomer. NMR solution structure shows that MCP-3 adopts an alphabeta fold similar to what is observed in structures of other known chemokines. However, MCP-3 is unique in that it does not show a propensity to form dimers. The closely related chemokines MCP-1 and MCP-2 show a monomer-dimer equilibrium in sedimentation equilibrium studies (approximately 0.2-2 mg/ml). As these proteins are present at nanomolar concentrations in vivo, the results suggest that they are monomeric at functional concentrations and that the monomer is the functionally significant form of MCP-1, MCP-2 and MCP-3.
 
  Selected figure(s)  
 
Figure 1.
Fig. 1. lignment of the primary amino acid sequence of MCP-1, MCP-2 and MCP-3. Disulfide bonds between conserved cysteines are indi- cated. 		Figure 6.
Fig. 6. Comparison of MCP-3 (thick black) with MIP-113 (thick gray) and monomeric L-8 black). The best-fit superpositions of the bac- bone atoms are shown.
 
  The above figures are reprinted by permission from the Federation of European Biochemical Societies: FEBS Lett (1996, 395, 277-282) copyright 1996.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21223963 C.L.Salanga, and T.M.Handel (2011).
Chemokine oligomerization and interactions with receptors and glycosaminoglycans: the role of structural dynamics in function.
  Exp Cell Res, 317, 590-601.  
19122657 R.R.Koenen, P.von Hundelshausen, I.V.Nesmelova, A.Zernecke, E.A.Liehn, A.Sarabi, B.K.Kramp, A.M.Piccinini, S.R.Paludan, M.A.Kowalska, A.J.Kungl, T.M.Hackeng, K.H.Mayo, and C.Weber (2009).
Disrupting functional interactions between platelet chemokines inhibits atherosclerosis in hyperlipidemic mice.
  Nat Med, 15, 97.  
18323622 C.Barinka, A.Prahl, and J.Lubkowski (2008).
Structure of human monocyte chemoattractant protein 4 (MCP-4/CCL13).
  Acta Crystallogr D Biol Crystallogr, 64, 273-278.
PDB code: 2ra4
17024562 L.Rajagopalan, and K.Rajarathnam (2006).
Structural basis of chemokine receptor function--a model for binding affinity and ligand selectivity.
  Biosci Rep, 26, 325-339.  
16803905 S.E.Crown, Y.Yu, M.D.Sweeney, J.A.Leary, and T.M.Handel (2006).
Heterodimerization of CCR2 chemokines and regulation by glycosaminoglycan binding.
  J Biol Chem, 281, 25438-25446.  
15979374 J.Y.Springael, E.Urizar, and M.Parmentier (2005).
Dimerization of chemokine receptors and its functional consequences.
  Cytokine Growth Factor Rev, 16, 611-623.  
16033763 Y.Yu, M.D.Sweeney, O.M.Saad, S.E.Crown, A.R.Hsu, T.M.Handel, and J.A.Leary (2005).
Chemokine-glycosaminoglycan binding: specificity for CCR2 ligand binding to highly sulfated oligosaccharides using FTICR mass spectrometry.
  J Biol Chem, 280, 32200-32208.  
15039444 V.Petkovic, C.Moghini, S.Paoletti, M.Uguccioni, and B.Gerber (2004).
Eotaxin-3/CCL26 is a natural antagonist for CC chemokine receptors 1 and 5. A human chemokine with a regulatory role.
  J Biol Chem, 279, 23357-23363.  
12571364 A.E.Proudfoot, T.M.Handel, Z.Johnson, E.K.Lau, P.LiWang, I.Clark-Lewis, F.Borlat, T.N.Wells, and M.H.Kosco-Vilbois (2003).
Glycosaminoglycan binding and oligomerization are essential for the in vivo activity of certain chemokines.
  Proc Natl Acad Sci U S A, 100, 1885-1890.  
11807180 E.J.Fernandez, and E.Lolis (2002).
Structure, function, and inhibition of chemokines.
  Annu Rev Pharmacol Toxicol, 42, 469-499.  
11470923 B.T.Seet, R.Singh, C.Paavola, E.K.Lau, T.M.Handel, and G.McFadden (2001).
Molecular determinants for CC-chemokine recognition by a poxvirus CC-chemokine inhibitor.
  Proc Natl Acad Sci U S A, 98, 9008-9013.  
11276085 C.Baysal, and A.R.Atilgan (2001).
Elucidating the structural mechanisms for biological activity of the chemokine family.
  Proteins, 43, 150-160.  
  11152129 Buyong, J.Xiong, J.Lubkowski, and R.Nussinov (2000).
Homology modeling and molecular dynamics simulations of lymphotactin.
  Protein Sci, 9, 2192-2199.  
11041848 E.J.Fernandez, J.Wilken, D.A.Thompson, S.C.Peiper, and E.Lolis (2000).
Comparison of the structure of vMIP-II with eotaxin-1, RANTES, and MCP-3 suggests a unique mechanism for CCR3 activation.
  Biochemistry, 39, 12837-12844.
PDB code: 1cm9
11087354 J.Blaszczyk, E.V.Coillie, P.Proost, J.V.Damme, G.Opdenakker, G.D.Bujacz, J.M.Wang, and X.Ji (2000).
Complete crystal structure of monocyte chemotactic protein-2, a CC chemokine that interacts with multiple receptors.
  Biochemistry, 39, 14075-14081.
PDB code: 1esr
10913244 K.L.Mayer, and M.J.Stone (2000).
NMR solution structure and receptor peptide binding of the CC chemokine eotaxin-2.
  Biochemistry, 39, 8382-8395.
PDB codes: 1eig 1eih
9890927 A.C.LiWang, J.J.Cao, H.Zheng, Z.Lu, S.C.Peiper, and P.J.LiWang (1999).
Dynamics study on the anti-human immunodeficiency virus chemokine viral macrophage-inflammatory protein-II (VMIP-II) reveals a fully monomeric protein.
  Biochemistry, 38, 442-453.  
  10595530 A.C.Liwang, Z.X.Wang, Y.Sun, S.C.Peiper, and P.J.Liwang (1999).
The solution structure of the anti-HIV chemokine vMIP-II.
  Protein Sci, 8, 2270-2280.
PDB code: 1vmp
  10379912 E.Van Coillie, J.Van Damme, and G.Opdenakker (1999).
The MCP/eotaxin subfamily of CC chemokines.
  Cytokine Growth Factor Rev, 10, 61-86.  
10320325 H.Sticht, S.E.Escher, K.Schweimer, W.G.Forssmann, P.Rösch, and K.Adermann (1999).
Solution structure of the human CC chemokine 2: A monomeric representative of the CC chemokine subtype.
  Biochemistry, 38, 5995-6002.
PDB code: 2hcc
9931005 L.S.Mizoue, J.F.Bazan, E.C.Johnson, and T.M.Handel (1999).
Solution structure and dynamics of the CX3C chemokine domain of fractalkine and its interaction with an N-terminal fragment of CX3CR1.
  Biochemistry, 38, 1402-1414.
PDB code: 1b2t
  10548050 M.P.Crump, L.Spyracopoulos, P.Lavigne, K.S.Kim, I.Clark-lewis, and B.D.Sykes (1999).
Backbone dynamics of the human CC chemokine eotaxin: fast motions, slow motions, and implications for receptor binding.
  Protein Sci, 8, 2041-2054.  
9837883 C.D.Paavola, S.Hemmerich, D.Grunberger, I.Polsky, A.Bloom, R.Freedman, M.Mulkins, S.Bhakta, D.McCarley, L.Wiesent, B.Wong, K.Jarnagin, and T.M.Handel (1998).
Monomeric monocyte chemoattractant protein-1 (MCP-1) binds and activates the MCP-1 receptor CCR2B.
  J Biol Chem, 273, 33157-33165.  
9649315 J.S.Laurence, A.C.LiWang, and P.J.LiWang (1998).
Effect of N-terminal truncation and solution conditions on chemokine dimer stability: nuclear magnetic resonance structural analysis of macrophage inflammatory protein 1 beta mutants.
  Biochemistry, 37, 9346-9354.  
9712872 M.P.Crump, K.Rajarathnam, K.S.Kim, I.Clark-Lewis, and B.D.Sykes (1998).
Solution structure of eotaxin, a chemokine that selectively recruits eosinophils in allergic inflammation.
  J Biol Chem, 273, 22471-22479.
PDB codes: 1eot 2eot
9622482 W.Shao, L.F.Jerva, J.West, E.Lolis, and B.I.Schweitzer (1998).
Solution structure of murine macrophage inflammatory protein-2.
  Biochemistry, 37, 8303-8313.
PDB code: 1mi2
9109648 S.Meunier, J.M.Bernassau, J.C.Guillemot, P.Ferrara, and H.Darbon (1997).
Determination of the three-dimensional structure of CC chemokine monocyte chemoattractant protein 3 by 1H two-dimensional NMR spectroscopy.
  Biochemistry, 36, 4412-4422.
PDB code: 1ncv
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.