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PDBsum entry 1bel

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protein ligands links
Hydrolase PDB id
1bel
Jmol
Contents
Protein chain
124 a.a. *
Ligands
SO4 ×2
MOH ×4
Waters ×82
* Residue conservation analysis
PDB id:
1bel
Name: Hydrolase
Title: Hydrolase phosphoric diester, RNA
Structure: Ribonuclease a. Chain: a. Other_details: ph 4.5
Source: Bos taurus. Cattle. Organism_taxid: 9913. Organ: pancreas
Resolution:
1.60Å     R-factor:   0.150    
Authors: M.H.Dung,J.A.Bell
Key ref:
M.H.Dung and J.A.Bell (1997). Structure of crystal form IX of bovine pancreatic ribonuclease A. Acta Crystallogr D Biol Crystallogr, 53, 419-425. PubMed id: 15299907 DOI: 10.1107/S0907444997000929
Date:
21-Dec-95     Release date:   14-Oct-96    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P61823  (RNAS1_BOVIN) -  Ribonuclease pancreatic
Seq:
Struc:
150 a.a.
124 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.1.27.5  - Pancreatic ribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in C-P or U-P with 2',3'-cyclic phosphate intermediates.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     metabolic process   3 terms 
  Biochemical function     nucleic acid binding     7 terms  

 

 
DOI no: 10.1107/S0907444997000929 Acta Crystallogr D Biol Crystallogr 53:419-425 (1997)
PubMed id: 15299907  
 
 
Structure of crystal form IX of bovine pancreatic ribonuclease A.
M.H.Dung, J.A.Bell.
 
  ABSTRACT  
 
The X-ray structure of crystal form IX of bovine pancre- atic ribonuclease A (space group P2(1)2(1)2(1)) is reported at 1.6 A resolution. The structure was refined to an R factor of 15.0% and includes coordinates for two sulfate ions, four methanol molecules and 82 waters. The structure could be superimposed on the highest resolution crystal structure of bovine pancreatic fibonuclease available (in space group P2(1)) with an r.m.s, difference in main-chain atomic positions of 0.51 A. Most of the larger differences between the two structures could be related to crystal lattice contacts. Superposition of the new structure with eight other structures of ribonuclease in six crystal forms resulted in an r.m.s, deviation from the average structure of 0.43 A for all main-chain atoms. This similarity among structures exists in spite of the fact that all nine molecules are in different crystal environments.
 
  Selected figure(s)  
 
Figure 2.
Fig. 2. Stereo pair of backbone tracing of the structures of nine independent molecules of ribonu- clease from seven different crystal forms. The two images show the same set of structures from oppo- site sides of the molecule. All other structures were superim- posed by linear least-squares fit to the form IX crystal structure. Color key: ribonuclease crystal form IX, blue (this work); 7RSA, purple (Wlodawer et al., 1988): IRSM, green-blue (Weber, Sheriff, Ohlendorf, Finzel & Sale- mme, 1985); 2RNS, magenta (Kim et al., 1992); 8RSA mole- cule A, red; 8RSA molecule B, red-orange (Nachman et al. , 1990); 4SRN, orange (deMel et al., 1992); IRBB molecule A, cyan: IRBB molecule B, green (Williams, Greene & McPherson, 1987).
 
  The above figure is reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (1997, 53, 419-425) copyright 1997.  
  Figure was selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
  10548049 J.A.Bell (1999).
X-ray crystal structures of a severely desiccated protein.
  Protein Sci, 8, 2033-2040.
PDB codes: 1c0b 1c0c
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