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DNA binding protein PDB id
1b8z
Jmol
Contents
Protein chains
67 a.a. *
Waters ×77
* Residue conservation analysis
PDB id:
1b8z
Name: DNA binding protein
Title: Hu from thermotoga maritima
Structure: Protein (histonelike protein hu). Chain: a, b. Engineered: yes
Source: Thermotoga maritima. Organism_taxid: 2336. Expressed in: escherichia coli. Expression_system_taxid: 562
Biol. unit: Dimer (from PQS)
Resolution:
1.60Å     R-factor:   0.215     R-free:   0.236
Authors: E.Christodoulou,W.R.Rypniewski,C.E.Vorgias
Key ref:
E.Christodoulou and C.E.Vorgias (1998). Cloning, overproduction, purification and crystallization of the DNA binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima. Acta Crystallogr D Biol Crystallogr, 54, 1043-1045. PubMed id: 9757133 DOI: 10.1107/S0907444998000341
Date:
03-Feb-99     Release date:   03-Feb-00    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P36206  (DBH_THEMA) -  DNA-binding protein HU
Seq:
Struc: 		90 a.a.
67 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     chromosome condensation   1 term 
  Biochemical function     DNA binding     1 term  

 

 
DOI no: 10.1107/S0907444998000341 Acta Crystallogr D Biol Crystallogr 54:1043-1045 (1998)
PubMed id: 9757133  
 
 
Cloning, overproduction, purification and crystallization of the DNA binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima.
E.Christodoulou, C.E.Vorgias.
 
  ABSTRACT  
 
The humar gene encoding for the histone-like DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima was efficiently overexpressed in Escherichia coli under the T7 promoter. The HU protein was purified using SP-Sepharose ion-exchange and heparin-affinity chromatography and was successfully crystallized in ammonium sulfate. The crystals were grown in the tetragonal form in space group P43 or P41 and have unit-cell dimensions a = b = 46.12, c = 77.56 A, alpha = beta = gamma = 90 degrees. The crystals diffract X-rays to 1.6 A resolution using synchrotron radiation and are suitable for determination of the HU structure at high resolution.
 
  Selected figure(s)  
 
Figure 2.
Fig. 2. Crystals of HUmar protein obtained as described in §3. Bar represents 1 mm.
 
  The above figure is reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (1998, 54, 1043-1045) copyright 1998.  
  Figure was selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
15066175 J.Ruiz-Sanz, V.V.Filimonov, E.Christodoulou, C.E.Vorgias, and P.L.Mateo (2004).
Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant.
  Eur J Biochem, 271, 1497-1507.  
12853489 K.K.Swinger, K.M.Lemberg, Y.Zhang, and P.A.Rice (2003).
Flexible DNA bending in HU-DNA cocrystal structures.
  EMBO J, 22, 3749-3760.
PDB codes: 1p51 1p71 1p78
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