PDBsum entry 1b8x

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protein links
Signal protein PDB id
Protein chain
260 a.a. *
* Residue conservation analysis
PDB id:
Name: Signal protein
Title: Glutathione s-transferase fused with the nuclear matrix targ signal of the transcription factor aml-1
Structure: Protein (aml-1b). Chain: a. Fragment: nmts fragment. Engineered: yes
Source: Escherichia coli. Organism_taxid: 562. Cell_line: bl21. Expressed in: escherichia coli. Expression_system_taxid: 562
Biol. unit: Monomer (from PDB file)
2.70Å     R-factor:   0.209     R-free:   0.310
Authors: L.Tang,B.Guo,A.J.Van Wijnen,J.B.Lian,J.L.Stein,G.S.Stein,G.W
Key ref: L.Tang et al. (1998). Preliminary crystallographic study of glutathione S-transferase fused with the nuclear matrix targeting signal of the transcription factor AML-1/CBF-alpha2. J Struct Biol, 123, 83-85. PubMed id: 9774548 DOI: 10.1006/jsbi.1998.4016
03-Feb-99     Release date:   12-Apr-99    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
P08515  (GST26_SCHJA) -  Glutathione S-transferase class-mu 26 kDa isozyme
218 a.a.
260 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.  - Glutathione transferase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: RX + glutathione = HX + R-S-glutathione
+ glutathione
= HX
+ R-S-glutathione
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     metabolic process   1 term 
  Biochemical function     transferase activity     2 terms  


DOI no: 10.1006/jsbi.1998.4016 J Struct Biol 123:83-85 (1998)
PubMed id: 9774548  
Preliminary crystallographic study of glutathione S-transferase fused with the nuclear matrix targeting signal of the transcription factor AML-1/CBF-alpha2.
L.Tang, B.Guo, A.J.van Wijnen, J.B.Lian, J.L.Stein, G.S.Stein, G.W.Zhou.
A glutathione S-transferase fused with the nuclear matrix targeting signal (GST-NMTS) of AML-1/CBF-alpha2 has been crystallized by the vapor diffusion method using polyethylene glycol (PEG) as the precipitant. The NMTS is a 31-amino-acid signal peptide that can target the AML-1/CBF-alpha2 protein to the nuclear matrix. The crystal belongs to tetragonal space group P43212 with unit cell dimensions a = b = 93.4 A, c = 57.6 A. There is one GST-fusion protein per asymmetric unit. Crystals diffracted to at least 2.7 A and are appropriate for structure determination.

Literature references that cite this PDB file's key reference

  PubMed id Reference
21184003 G.S.Stein, J.L.Stein, A.J.van Wijnen, J.B.Lian, S.K.Zaidi, J.A.Nickerson, M.A.Montecino, and D.W.Young (2011).
An architectural genetic and epigenetic perspective.
  Integr Biol (Camb), 3, 297-303.  
14743389 G.S.Stein, J.B.Lian, A.J.van Wijnen, J.L.Stein, A.Javed, M.Montecino, S.K.Zaidi, D.Young, J.Y.Choi, S.Gutierrez, and S.Pockwinse (2004).
Nuclear microenvironments support assembly and organization of the transcriptional regulatory machinery for cell proliferation and differentiation.
  J Cell Biochem, 91, 287-302.  
12520536 G.S.Stein, J.B.Lian, J.L.Stein, A.J.Wijnen, M.Montecino, A.Javed, J.Pratap, J.Choi, S.K.Zaidi, S.Gutierrez, K.Harrington, J.Shen, and D.Young (2003).
Intranuclear trafficking of transcription factors: Requirements for vitamin D-mediated biological control of gene expression.
  J Cell Biochem, 88, 340-355.  
10898331 G.S.Stein, J.B.Lian, J.L.Stein, and A.J.van Wijnen (2000).
Bone tissue specific transcriptional control: options for targeting gene therapy to the skeleton.
  Cancer, 88, 2899-2902.  
10679814 J.A.Gordon, S.M.Pockwinse, F.M.Stewart, P.J.Quesenberry, T.Nakamura, C.M.Croce, J.B.Lian, J.L.Stein, A.J.van Wijnen, and G.S.Stein (2000).
Modified intranuclear organization of regulatory factors in human acute leukemias: reversal after treatment.
  J Cell Biochem, 77, 30-43.  
10497303 G.S.Stein, A.J.Van Wijnen, M.Montecino, J.L.Stein, and J.B.Lian (1999).
Nuclear structure/gene expression interrelationships.
  J Cell Physiol, 181, 240-250.  
10559245 L.Tang, B.Guo, A.Javed, J.Y.Choi, S.Hiebert, J.B.Lian, A.J.van Wijnen, J.L.Stein, G.S.Stein, and G.W.Zhou (1999).
Crystal structure of the nuclear matrix targeting signal of the transcription factor acute myelogenous leukemia-1/polyoma enhancer-binding protein 2alphaB/core binding factor alpha2.
  J Biol Chem, 274, 33580-33586.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.