PDBsum entry 1axn

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Calcium/phospholipid-binding protein PDB id
Protein chain
323 a.a. *
_CA ×5
Waters ×281
* Residue conservation analysis
PDB id:
Name: Calcium/phospholipid-binding protein
Title: The high resolution structure of annexin iii shows differences with annexin v
Structure: Annexin iii. Chain: a. Engineered: yes. Other_details: human recombinant
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: human annexin iii. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: prepared as a gst-fusion, then cleaved yes
1.78Å     R-factor:   0.177     R-free:   0.221
Authors: B.Favier-Perron,A.Lewit-Bentley,F.Russo-Marie
Key ref:
B.Favier-Perron et al. (1996). The high-resolution crystal structure of human annexin III shows subtle differences with annexin V. Biochemistry, 35, 1740-1744. PubMed id: 8639653 DOI: 10.1021/bi952092o
21-Aug-95     Release date:   08-Mar-96    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
P12429  (ANXA3_HUMAN) -  Annexin A3
323 a.a.
323 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   6 terms 
  Biological process     negative regulation of catalytic activity   7 terms 
  Biochemical function     calcium-dependent protein binding     5 terms  


DOI no: 10.1021/bi952092o Biochemistry 35:1740-1744 (1996)
PubMed id: 8639653  
The high-resolution crystal structure of human annexin III shows subtle differences with annexin V.
B.Favier-Perron, A.Lewit-Bentley, F.Russo-Marie.
The structure of recombinant human annexin III was solved to 1.8 A resolution. Though homologous to annexin I and V, the annexin III structure shows significant differences. The tryptophan in the calcium loop of the third domain is exposed to the solvent, as in the structure of annexin V crystallized in high calcium concentrations, although the annexin III crystals were prepared at low calcium concentrations. The position of domain III relative to the other domains is different from both annexin V and I, suggesting further flexibility of the molecule. The entire N-terminus of the protein is well-defined in the present structure. The side chain of tryptophan 5 interacts with the hinge region of the hydrophillic channel, which could have an effect on the potential mobility of this region, as well as on its possible calcium channel behavior.

Literature references that cite this PDB file's key reference

  PubMed id Reference
  21348939 L.Zhou, J.Duan, X.M.Wang, H.M.Zhang, M.X.Duan, and J.Y.Liu (2011).
Characterization of a novel annexin gene from cotton (Gossypium hirsutum cv CRI 35) and antioxidative role of its recombinant protein.
  J Integr Plant Biol, 53, 347-357.  
19381436 K.Monastyrskaya, E.B.Babiychuk, and A.Draeger (2009).
The annexins: spatial and temporal coordination of signaling events during cellular stress.
  Cell Mol Life Sci, 66, 2623-2642.  
15904872 D.G.Naidu, A.Raha, X.L.Chen, A.R.Spitzer, and A.Chander (2005).
Partial truncation of the NH2-terminus affects physical characteristics and membrane binding, aggregation, and fusion properties of annexin A7.
  Biochim Biophys Acta, 1734, 152-168.  
15280367 M.Jin, C.Smith, H.Y.Hsieh, D.F.Gibson, and J.F.Tait (2004).
Essential role of B-helix calcium binding sites in annexin V-membrane binding.
  J Biol Chem, 279, 40351-40357.  
12070314 J.Sopkova, C.Raguenes-Nicol, M.Vincent, A.Chevalier, A.Lewit-Bentley, F.Russo-Marie, and J.Gallay (2002).
Ca(2+) and membrane binding to annexin 3 modulate the structure and dynamics of its N terminus and domain III.
  Protein Sci, 11, 1613-1625.  
11948176 P.Montaville, J.M.Neumann, F.Russo-Marie, F.Ochsenbein, and A.Sanson (2002).
A new consensus sequence for phosphatidylserine recognition by annexins.
  J Biol Chem, 277, 24684-24693.  
12124256 T.Huynh, J.C.Smith, and A.Sanson (2002).
Protein unfolding transitions in an intrinsically unstable annexin domain: molecular dynamics simulation and comparison with nuclear magnetic resonance data.
  Biophys J, 83, 681-698.  
10869176 A.Hofmann, C.Raguénès-Nicol, B.Favier-Perron, J.Mesonero, R.Huber, F.Russo-Marie, and A.Lewit-Bentley (2000).
The annexin A3-membrane interaction is modulated by an N-terminal tryptophan.
  Biochemistry, 39, 7712-7721.  
11053851 A.Rosengarth, and H.Luecke (2000).
Crystallization and preliminary X-ray analysis of full-length annexin I comprising the core and N-terminal domain.
  Acta Crystallogr D Biol Crystallogr, 56, 1459-1461.  
11087353 J.Sopkova-De Oliveira Santos, S.Fischer, C.Guilbert, A.Lewit-Bentley, and J.C.Smith (2000).
Pathway for large-scale conformational change in annexin V.
  Biochemistry, 39, 14065-14074.  
10353474 F.Russo-Marie (1999).
Annexin V and phospholipid metabolism.
  Clin Chem Lab Med, 37, 287-291.  
10220332 J.Sopkova, M.Vincent, M.Takahashi, A.Lewit-Bentley, and J.Gallay (1999).
Conformational flexibility of domain III of annexin V at membrane/water interfaces.
  Biochemistry, 38, 5447-5458.  
9446599 A.Hofmann, A.Escherich, A.Lewit-Bentley, J.Benz, C.Raguenes-Nicol, F.Russo-Marie, V.Gerke, L.Moroder, and R.Huber (1998).
Interactions of benzodiazepine derivatives with annexins.
  J Biol Chem, 273, 2885-2894.  
9718321 J.Sopkova, M.Vincent, M.Takahashi, A.Lewit-Bentley, and J.Gallay (1998).
Conformational flexibility of domain III of annexin V studied by fluorescence of tryptophan 187 and circular dichroism: the effect of pH.
  Biochemistry, 37, 11962-11970.  
9511742 M.P.Fernández, N.G.Copeland, D.J.Gilbert, N.A.Jenkins, and R.O.Morgan (1998).
Mouse annexin III cDNA, genetic mapping and evolution.
  Gene, 207, 43-51.  
9111038 B.Perron, A.Lewit-Bentley, B.Geny, and F.Russo-Marie (1997).
Can enzymatic activity, or otherwise, be inferred from structural studies of annexin III?
  J Biol Chem, 272, 11321-11326.
PDB code: 1aii
9182528 G.Kassam, A.Manro, C.E.Braat, P.Louie, S.L.Fitzpatrick, and D.M.Waisman (1997).
Characterization of the heparin binding properties of annexin II tetramer.
  J Biol Chem, 272, 15093-15100.  
9047302 H.M.Kang, G.Kassam, S.E.Jarvis, S.L.Fitzpatrick, and D.M.Waisman (1997).
Characterization of human recombinant annexin II tetramer purified from bacteria: role of N-terminal acetylation.
  Biochemistry, 36, 2041-2050.  
  17708913 S.E.Moss (1997).
  Trends Cell Biol, 7, 87-89.  
9223619 V.Gerke, and S.E.Moss (1997).
Annexins and membrane dynamics.
  Biochim Biophys Acta, 1357, 129-154.  
  9165062 W.Baumeister, Z.Cejka, M.Kania, and E.Seemüller (1997).
The proteasome: a macromolecular assembly designed to confine proteolysis to a nanocompartment.
  Biol Chem, 378, 121-130.  
9220993 W.S.Mailliard, H.Luecke, and H.T.Haigler (1997).
Annexin XII forms calcium-dependent multimers in solution and on phospholipid bilayers: a chemical cross-linking study.
  Biochemistry, 36, 9045-9050.  
8756690 F.Cordier-Ochsenbein, R.Guerois, F.Baleux, T.Huynh-Dinh, A.Chaffotte, J.M.Neumann, and A.Sanson (1996).
Folding properties of an annexin I domain: a 1H-15N NMR and CD study.
  Biochemistry, 35, 10347-10357.  
8898865 H.Kawasaki, A.Avila-Sakar, C.E.Creutz, and R.H.Kretsinger (1996).
The crystal structure of annexin VI indicates relative rotation of the two lobes upon membrane binding.
  Biochim Biophys Acta, 1313, 277-282.  
8898867 T.Dubois, J.P.Oudinet, J.P.Mira, and F.Russo-Marie (1996).
Annexins and protein kinases C.
  Biochim Biophys Acta, 1313, 290-294.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.