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Hydrolase PDB id
1awz
Jmol
Contents
Protein chain
123 a.a. *
* Residue conservation analysis
PDB id:
1awz
Name: Hydrolase
Title: 3d solution structure of human angiogenin determined by 1h, 15n nmr spectroscopy, 30 structures
Structure: Angiogenin. Chain: a. Engineered: yes. Other_details: pancreatic ribonuclease superfamily
Source: Homo sapiens. Human. Organism_taxid: 9606. Tissue: plasma. Expressed in: escherichia coli b. Expression_system_taxid: 37762.
NMR struc: 30 models
Authors: O.Lequin,H.Thuring,M.Robin,J.-Y.Lallemand
Key ref: O.Lequin et al. (1997). Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values. Eur J Biochem, 250, 712-726. PubMed id: 9461294 DOI: 10.1111/j.1432-1033.1997.00712.x
Date:
07-Oct-97     Release date:   25-Feb-98    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P03950  (ANGI_HUMAN) -  Angiogenin
Seq:
Struc: 		147 a.a.
123 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   8 terms 
  Biological process     metabolic process   24 terms 
  Biochemical function     nucleic acid binding     14 terms  

 

 
DOI no: 10.1111/j.1432-1033.1997.00712.x Eur J Biochem 250:712-726 (1997)
PubMed id: 9461294  
 
 
Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values.
O.Lequin, H.Thüring, M.Robin, J.Y.Lallemand.
 
  ABSTRACT  
 
Human angiogenin is a member of the pancreatic ribonuclease superfamily that induces blood vessel formation. Its three-dimensional solution structure has been determined to high resolution by heteronuclear NMR spectroscopy. 30 structures were calculated, based on a total of 1441 assigned NOE correlations, 64 coupling constants and 50 hydrogen bonds. The backbone atomic rms difference from the mean coordinates is 0.067 +/- 0.012 nm and 0.13 nm from the previously determined crystal structure. The side-chain of Gln117 was found to obstruct the active site as observed in the crystal state. There was no evidence of an alternative open form of angiogenin, although two sets of chemical shifts were observed for some residues, mainly around the active site and in the C-terminal segment. The topology of the ribonucleolytic active site is described with a particular emphasis on the conformation and protonation of active-site His residues. The side-chain of His114 adopts two main conformations in solution. In contrast to pancreatic ribonuclease A, His13 was shown to be more basic than His114, with pKa values of 6.65 and 6.05 respectively. The His47 residue is located in an environment very resistant to protonation with a pKa lower than 4.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20941439 D.La Mendola, A.Magrì, L.I.Vagliasindi, Ã.–.Hansson, R.P.Bonomo, and E.Rizzarelli (2010).
Copper(II) complex formation with a linear peptide encompassing the putative cell binding site of angiogenin.
  Dalton Trans, 39, 10678-10684.  
20653509 V.N.Uversky (2010).
Targeting intrinsically disordered proteins in neurodegenerative and protein dysfunction diseases: another illustration of the D(2) concept.
  Expert Rev Proteomics, 7, 543-564.  
16301790 D.E.Holloway, G.B.Chavali, M.C.Hares, V.Subramanian, and K.R.Acharya (2005).
Structure of murine angiogenin: features of the substrate- and cell-binding regions and prospects for inhibitor-binding studies.
  Acta Crystallogr D Biol Crystallogr, 61, 1568-1578.
PDB codes: 2bwk 2bwl
15772759 Y.R.Pan, K.P.Wu, Y.C.Lou, Y.D.Liao, and C.Chen (2005).
1H, 13C and 15N resonance assignments and secondary structure of murine angiogenin 4.
  J Biomol NMR, 31, 175-176.  
12945053 A.Merlino, L.Vitagliano, M.A.Ceruso, and L.Mazzarella (2003).
Subtle functional collective motions in pancreatic-like ribonucleases: from ribonuclease A to angiogenin.
  Proteins, 53, 101-110.  
  12842050 G.B.Chavali, A.C.Papageorgiou, K.A.Olson, J.W.Fett, G.Hu, R.Shapiro, and K.R.Acharya (2003).
The crystal structure of human angiogenin in complex with an antitumor neutralizing antibody.
  Structure, 11, 875-885.
PDB code: 1h0d
11468363 D.D.Leonidas, G.B.Chavali, A.M.Jardine, S.Li, R.Shapiro, and K.R.Acharya (2001).
Binding of phosphate and pyrophosphate ions at the active site of human angiogenin as revealed by X-ray crystallography.
  Protein Sci, 10, 1669-1676.
PDB codes: 1h52 1h53 1hby
11093266 M.S.Madhusudhan, and S.Vishveshwara (2001).
Computer modeling of human angiogenin-dinucleotide substrate interaction.
  Proteins, 42, 125-135.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.