PDBsum entry: 1aw6

Transcription regulation

PDB id: 1aw6

Contents

Protein chain

43 a.a. *

Metals

_CD ×2

* Residue conservation analysis

PDB id:

1aw6

Name:

Transcription regulation

Title:

Gal4 (cd), nmr, 24 structures

Structure:

Gal4 (cd). Chain: a. Fragment: DNA-binding domain. Engineered: yes

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

24 models

Authors:

J.D.Baleja,G.Wagner

Key ref:

J.D.Baleja et al. (1997). Refined solution structure of the DNA-binding domain of GAL4 and use of 3J(113Cd,1H) in structure determination. J Biomol Nmr, 10, 397-401. PubMed id: 9460244 DOI: 10.1023/A:1018332327565

Date:

10-Oct-97 Release date: 15-Apr-98

Supersedes:

125d

Protein chain

P04386  (GAL4_YEAST) -  Regulatory protein GAL4

Seq: 881 a.a.

Struc: 43 a.a.

 Gene Ontology (GO) functional annotation 

• Cellular component: nucleus (1 term)
• Biological process: regulation of transcription, DNA-dependent (1 term)
• Biochemical function: transcription factor activity (2 terms)

DOI no: 10.1023/A:1018332327565

J Biomol Nmr 10:397-401 (1997)

PubMed id: 9460244

Refined solution structure of the DNA-binding domain of GAL4 and use of 3J(113Cd,1H) in structure determination.

J.D.Baleja, V.Thanabal, G.Wagner.

ABSTRACT

We have refined the solution structure of cadmium-bound GAL4 and present its 15N and 1H NMR assignments. The root-mean-square (rms) deviation to the average structure was 0.4 +/- 0.05 A for backbone atoms, and 0.9 +/- 0.1 A for all heavy atoms. The three-bond heteronuclear 3J(113Cd,1H) coupling constants were found to disobey a Karplus-type relationship, which was attributable to the unusual constraints imposed by the bimetal-thiolate cluster in GAL4. We conclude that the structural parameters that correlate to 3J(113Cd,1H) are complex.