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PDBsum entry 1avc

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protein metals links
Calcium/phospholipid-binding protein PDB id
1avc
Jmol
Contents
Protein chain
642 a.a. *
Metals
_CA ×6
Waters ×117
* Residue conservation analysis
PDB id:
1avc
Name: Calcium/phospholipid-binding protein
Title: Bovine annexin vi (calcium-bound)
Structure: Annexin vi. Chain: a. Synonym: p68, protein iii, 67-kda-calcimedin, lipocortin vi, 67-kda-calelectrin, chromobindin 20, calphobindin ii
Source: Bos taurus. Cattle. Organism_taxid: 9913. Organ: liver. Cellular_location: membrane-peripheral
Resolution:
2.90Å     R-factor:   0.205     R-free:   0.268
Authors: A.J.Avila-Sakar,C.E.Creutz,R.H.Kretsinger
Key ref: A.J.Avila-Sakar et al. (1998). Crystal structure of bovine annexin VI in a calcium-bound state. Biochim Biophys Acta, 1387, 103-116. PubMed id: 9748523 DOI: 10.1016/S0167-4838(98)00111-3
Date:
16-Sep-97     Release date:   28-Jan-98    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P79134  (ANXA6_BOVIN) -  Annexin A6
Seq:
Struc:
 
Seq:
Struc:
673 a.a.
642 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 8 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   12 terms 
  Biological process     growth plate cartilage chondrocyte differentiation   5 terms 
  Biochemical function     chondroitin sulfate binding     10 terms  

 

 
DOI no: 10.1016/S0167-4838(98)00111-3 Biochim Biophys Acta 1387:103-116 (1998)
PubMed id: 9748523  
 
 
Crystal structure of bovine annexin VI in a calcium-bound state.
A.J.Avila-Sakar, C.E.Creutz, R.H.Kretsinger.
 
  ABSTRACT  
 
The crystal structure of a calcium-bound form of bovine annexin VI has been determined with X-ray diffraction data to 2.9 A by molecular replacement. Six Ca2+ ions were found, five in AB loops, one in a DE loop. Two loops (II-AB, which binds calcium, and V-AB, which does not) have conformations that differ significantly from those in calcium-free, human recombinant annexin VI. There are only small differences between the calci- and the apo-annexin VI in the rest of the molecule. Calcium by itself does not promote a major conformational change.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20888375 C.Enrich, C.Rentero, S.V.de Muga, M.Reverter, V.Mulay, P.Wood, M.Koese, and T.Grewal (2011).
Annexin A6-Linking Ca(2+) signaling with cholesterol transport.
  Biochim Biophys Acta, 1813, 935-947.  
18795951 A.C.Rintala-Dempsey, A.Rezvanpour, and G.S.Shaw (2008).
S100-annexin complexes--structural insights.
  FEBS J, 275, 4956-4966.  
17613532 B.R.Genge, L.N.Wu, and R.E.Wuthier (2007).
In vitro modeling of matrix vesicle nucleation: synergistic stimulation of mineral formation by annexin A5 and phosphatidylserine.
  J Biol Chem, 282, 26035-26045.  
17355882 P.Pathuri, E.T.Nguyen, S.G.Svärd, and H.Luecke (2007).
Apo and calcium-bound crystal structures of Alpha-11 giardin, an unusual annexin from Giardia lamblia.
  J Mol Biol, 368, 493-508.
PDB codes: 2ii2 2iic
17267400 T.Fischer, L.Lu, H.T.Haigler, and R.Langen (2007).
Annexin B12 is a sensor of membrane curvature and undergoes major curvature-dependent structural changes.
  J Biol Chem, 282, 9996.  
15904872 D.G.Naidu, A.Raha, X.L.Chen, A.R.Spitzer, and A.Chander (2005).
Partial truncation of the NH2-terminus affects physical characteristics and membrane binding, aggregation, and fusion properties of annexin A7.
  Biochim Biophys Acta, 1734, 152-168.  
15928709 V.Gerke, C.E.Creutz, and S.E.Moss (2005).
Annexins: linking Ca2+ signalling to membrane dynamics.
  Nat Rev Mol Cell Biol, 6, 449-461.  
15388925 A.Korostelev, M.O.Fenley, and M.S.Chapman (2004).
Impact of a Poisson-Boltzmann electrostatic restraint on protein structures refined at medium resolution.
  Acta Crystallogr D Biol Crystallogr, 60, 1786-1794.  
15298924 M.Golczak, A.Kirilenko, J.Bandorowicz-Pikula, B.Desbat, and S.Pikula (2004).
Structure of human annexin a6 at the air-water interface and in a membrane-bound state.
  Biophys J, 87, 1215-1226.  
12401794 Y.Mo, B.Campos, T.R.Mealy, L.Commodore, J.F.Head, J.R.Dedman, and B.A.Seaton (2003).
Interfacial basic cluster in annexin V couples phospholipid binding and trimer formation on membrane surfaces.
  J Biol Chem, 278, 2437-2443.
PDB codes: 1n41 1n42 1n44
11964259 A.Kirilenko, M.Golczak, S.Pikula, R.Buchet, and J.Bandorowicz-Pikula (2002).
GTP-induced membrane binding and ion channel activity of annexin VI: is annexin VI a GTP biosensor?
  Biophys J, 82, 2737-2745.  
12021440 F.Fabiola, R.Bertram, A.Korostelev, and M.S.Chapman (2002).
An improved hydrogen bond potential: impact on medium resolution protein structures.
  Protein Sci, 11, 1415-1423.  
12070178 I.de Diego, F.Schwartz, H.Siegfried, P.Dauterstedt, J.Heeren, U.Beisiegel, C.Enrich, and T.Grewal (2002).
Cholesterol modulates the membrane binding and intracellular distribution of annexin 6.
  J Biol Chem, 277, 32187-32194.  
11948176 P.Montaville, J.M.Neumann, F.Russo-Marie, F.Ochsenbein, and A.Sanson (2002).
A new consensus sequence for phosphatidylserine recognition by annexins.
  J Biol Chem, 277, 24684-24693.  
10492018 M.Danieluk, S.Pikuła, and J.Bandorowicz-Pikuła (1999).
Annexin VI interacts with adenine nucleotides and their analogs.
  Biochimie, 81, 717-726.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.