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Hydrolase (vascularization) PDB id
1ang
Jmol
Contents
Protein chain
123 a.a. *
* Residue conservation analysis
PDB id:
1ang
Name: Hydrolase (vascularization)
Title: Crystal structure of human angiogenin reveals the structural basis for its functional divergence from ribonuclease
Structure: Angiogenin. Chain: a. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
2.40Å     R-factor:   0.220    
Authors: K.R.Acharya,S.Allen,R.Shapiro,J.F.Riordan,B.L.Vallee
Key ref: K.R.Acharya et al. (1994). Crystal structure of human angiogenin reveals the structural basis for its functional divergence from ribonuclease. Proc Natl Acad Sci U S A, 91, 2915-2919. PubMed id: 8159679 DOI: 10.1073/pnas.91.8.2915
Date:
18-Jan-94     Release date:   20-Apr-95    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P03950  (ANGI_HUMAN) -  Angiogenin
Seq:
Struc: 		147 a.a.
123 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   8 terms 
  Biological process     metabolic process   24 terms 
  Biochemical function     nucleic acid binding     14 terms  

 

 
DOI no: 10.1073/pnas.91.8.2915 Proc Natl Acad Sci U S A 91:2915-2919 (1994)
PubMed id: 8159679  
 
 
Crystal structure of human angiogenin reveals the structural basis for its functional divergence from ribonuclease.
K.R.Acharya, R.Shapiro, S.C.Allen, J.F.Riordan, B.L.Vallee.
 
  ABSTRACT  
 
Angiogenin, a potent inducer of neovascularization, is the only angiogenic molecule known to exhibit ribonucleolytic activity. Its overall structure, as determined at 2.4 A, is similar to that of pancreatic ribonuclease A, but it differs markedly in several distinct areas, particularly the ribonucleolytic active center and the putative receptor binding site, both of which are critically involved in biological function. Most strikingly, the site that is spatially analogous to that for pyrimidine binding in ribonuclease A differs significantly in conformation and is "obstructed" by glutamine-117. Movement of this and adjacent residues may be required for substrate binding to angiogenin and, hence, constitute a key part of its mechanism of action.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21050179 E.Pizzo, A.Merlino, M.Turano, I.Russo Krauss, F.Coscia, A.Zanfardino, M.Varcamonti, A.Furia, C.Giancola, L.Mazzarella, F.Sica, and G.D'Alessio (2010).
A new RNase sheds light on the RNase/angiogenin subfamily from zebrafish.
  Biochem J, 433, 345-355.
PDB codes: 3ljd 3lje 3ln8
20950426 F.M.Codoñer, S.Alfonso-Loeches, and M.A.Fares (2010).
Mutational dynamics of murine angiogenin duplicates.
  BMC Evol Biol, 10, 310.  
20045391 M.G.Pyatibratov, D.Tolkatchev, J.Plamondon, P.Xu, F.Ni, and A.S.Kostyukova (2010).
Binding of human angiogenin inhibits actin polymerization.
  Arch Biochem Biophys, 495, 74-81.  
20675404 R.Tomecki, and A.Dziembowski (2010).
Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism.
  RNA, 16, 1692-1724.  
19968858 W.M.Li, T.Barnes, and C.H.Lee (2010).
Endoribonucleases--enzymes gaining spotlight in mRNA metabolism.
  FEBS J, 277, 627-641.  
19191310 D.E.Holloway, G.B.Chavali, D.D.Leonidas, M.D.Baker, and K.R.Acharya (2009).
Influence of naturally-occurring 5'-pyrophosphate-linked substituents on the binding of adenylic inhibitors to ribonuclease a: An X-ray crystallographic study.
  Biopolymers, 91, 995.
PDB codes: 2w5g 2w5i 2w5k 2w5l 2w5m
19549190 D.M.Monti, W.Yu, E.Pizzo, K.Shima, M.G.Hu, C.Di Malta, R.Piccoli, G.D'Alessio, and G.F.Hu (2009).
Characterization of the angiogenic activity of zebrafish ribonucleases.
  FEBS J, 276, 4077-4090.  
18087731 C.Gellera, C.Colombrita, N.Ticozzi, B.Castellotti, C.Bragato, A.Ratti, F.Taroni, and V.Silani (2008).
Identification of new ANG gene mutations in a large cohort of Italian patients with amyotrophic lateral sclerosis.
  Neurogenetics, 9, 33-40.  
18781822 H.Kishikawa, D.Wu, and G.F.Hu (2008).
Targeting angiogenin in therapy of amyotropic lateral sclerosis.
  Expert Opin Ther Targets, 12, 1229-1242.  
18508078 K.Kazakou, D.E.Holloway, S.H.Prior, V.Subramanian, and K.R.Acharya (2008).
Ribonuclease A homologues of the zebrafish: polymorphism, crystal structures of two representatives and their evolutionary implications.
  J Mol Biol, 380, 206-222.
PDB codes: 2vq8 2vq9
18604453 X.Gao, and Z.Xu (2008).
Mechanisms of action of angiogenin.
  Acta Biochim Biophys Sin (Shanghai), 40, 619-624.  
17883850 D.S.Osorio, A.Antunes, and M.J.Ramos (2007).
Structural and functional implications of positive selection at the primate angiogenin gene.
  BMC Evol Biol, 7, 167.  
16961595 A.Tello-Montoliu, J.V.Patel, and G.Y.Lip (2006).
Angiogenin: a review of the pathophysiology and potential clinical applications.
  J Thromb Haemost, 4, 1864-1874.  
16433931 H.T.Chang, T.W.Pai, T.C.Fan, B.H.Su, P.C.Wu, C.Y.Tang, C.T.Chang, S.H.Liu, and M.D.Chang (2006).
A reinforced merging methodology for mapping unique peptide motifs in members of protein families.
  BMC Bioinformatics, 7, 38.  
16501576 M.J.Greenway, P.M.Andersen, C.Russ, S.Ennis, S.Cashman, C.Donaghy, V.Patterson, R.Swingler, D.Kieran, J.Prehn, K.E.Morrison, A.Green, K.R.Acharya, R.H.Brown, and O.Hardiman (2006).
ANG mutations segregate with familial and 'sporadic' amyotrophic lateral sclerosis.
  Nat Genet, 38, 411-413.  
15746964 A.K.Dudani, M.Ben-Tchavtchavadze, S.Porter, and E.Tackaberry (2005).
Angiostatin and plasminogen share binding to endothelial cell surface actin.
  Biochem Cell Biol, 83, 28-35.  
16301790 D.E.Holloway, G.B.Chavali, M.C.Hares, V.Subramanian, and K.R.Acharya (2005).
Structure of murine angiogenin: features of the substrate- and cell-binding regions and prospects for inhibitor-binding studies.
  Acta Crystallogr D Biol Crystallogr, 61, 1568-1578.
PDB codes: 2bwk 2bwl
12945053 A.Merlino, L.Vitagliano, M.A.Ceruso, and L.Mazzarella (2003).
Subtle functional collective motions in pancreatic-like ribonucleases: from ribonuclease A to angiogenin.
  Proteins, 53, 101-110.  
  12842050 G.B.Chavali, A.C.Papageorgiou, K.A.Olson, J.W.Fett, G.Hu, R.Shapiro, and K.R.Acharya (2003).
The crystal structure of human angiogenin in complex with an antitumor neutralizing antibody.
  Structure, 11, 875-885.
PDB code: 1h0d
12471601 J.L.Jenkins, R.Y.Kao, and R.Shapiro (2003).
Virtual screening to enrich hit lists from high-throughput screening: a case study on small-molecule inhibitors of angiogenin.
  Proteins, 50, 81-93.  
11468363 D.D.Leonidas, G.B.Chavali, A.M.Jardine, S.Li, R.Shapiro, and K.R.Acharya (2001).
Binding of phosphate and pyrophosphate ions at the active site of human angiogenin as revealed by X-ray crystallography.
  Protein Sci, 10, 1669-1676.
PDB codes: 1h52 1h53 1hby
11264578 J.Pous, G.Mallorquí-Fernández, R.Peracaula, S.S.Terzyan, J.Futami, H.Tada, H.Yamada, M.Seno, R.de Llorens, F.X.Gomis-Rüth, and M.Coll (2001).
Three-dimensional structure of human RNase 1 delta N7 at 1.9 A resolution.
  Acta Crystallogr D Biol Crystallogr, 57, 498-505.
PDB code: 1e21
11093266 M.S.Madhusudhan, and S.Vishveshwara (2001).
Computer modeling of human angiogenin-dinucleotide substrate interaction.
  Proteins, 42, 125-135.  
10070263 M.S.Madhusudhan, and S.Vishveshwara (1999).
Comparison of the dynamics of bovine and human angiogenin: a molecular dynamics study.
  Biopolymers, 49, 131-144.  
10090281 V.Gupta, S.Muyldermans, L.Wyns, and D.M.Salunke (1999).
The crystal structure of recombinant rat pancreatic RNase A.
  Proteins, 35, 1.
PDB code: 1rra
9649305 J.Hofsteenge, C.Moldow, A.M.Vicentini, O.Zelenko, Z.Jarai-Kote, and U.Neumann (1998).
A single amino acid substitution changes ribonuclease 4 from a uridine-specific to a cytidine-specific enzyme.
  Biochemistry, 37, 9250-9257.  
9539780 R.Piccoli, K.A.Olson, B.L.Vallee, and J.W.Fett (1998).
Chimeric anti-angiogenin antibody cAb 26-2F inhibits the formation of human breast cancer xenografts in athymic mice.
  Proc Natl Acad Sci U S A, 95, 4579-4583.  
9578571 R.Shapiro (1998).
Structural features that determine the enzymatic potency and specificity of human angiogenin: threonine-80 and residues 58-70 and 116-123.
  Biochemistry, 37, 6847-6856.  
9578572 V.Nobile, N.Russo, G.Hu, and J.F.Riordan (1998).
Inhibition of human angiogenin by DNA aptamers: nuclear colocalization of an angiogenin-inhibitor complex.
  Biochemistry, 37, 6857-6863.  
9311977 A.C.Papageorgiou, R.Shapiro, and K.R.Acharya (1997).
Molecular recognition of human angiogenin by placental ribonuclease inhibitor--an X-ray crystallographic study at 2.0 A resolution.
  EMBO J, 16, 5162-5177.
PDB code: 1a4y
9050852 C.Z.Chen, and R.Shapiro (1997).
Site-specific mutagenesis reveals differences in the structural bases for tight binding of RNase inhibitor to angiogenin and RNase A.
  Proc Natl Acad Sci U S A, 94, 1761-1766.  
9154942 D.D.Leonidas, R.Shapiro, L.I.Irons, N.Russo, and K.R.Acharya (1997).
Crystal structures of ribonuclease A complexes with 5'-diphosphoadenosine 3'-phosphate and 5'-diphosphoadenosine 2'-phosphate at 1.7 A resolution.
  Biochemistry, 36, 5578-5588.
PDB codes: 1afk 1afl 1afu
9266695 D.J.Strydom, M.D.Bond, and B.L.Vallee (1997).
An angiogenic protein from bovine serum and milk--purification and primary structure of angiogenin-2.
  Eur J Biochem, 247, 535-544.  
9122172 G.F.Hu, J.F.Riordan, and B.L.Vallee (1997).
A putative angiogenin receptor in angiogenin-responsive human endothelial cells.
  Proc Natl Acad Sci U S A, 94, 2204-2209.  
9461294 O.Lequin, H.Thüring, M.Robin, and J.Y.Lallemand (1997).
Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values.
  Eur J Biochem, 250, 712-726.
PDB code: 1awz
  9032278 X.Fu, and M.P.Kamps (1997).
E2a-Pbx1 induces aberrant expression of tissue-specific and developmentally regulated genes when expressed in NIH 3T3 fibroblasts.
  Mol Cell Biol, 17, 1503-1512.  
8703917 A.M.Vicentini, Z.Kote-Jarai, and J.Hofsteenge (1996).
Structural determinants of the uridine-preferring specificity of RNase PL3.
  Biochemistry, 35, 9128-9132.  
8570639 N.Russo, K.R.Acharya, B.L.Vallee, and R.Shapiro (1996).
A combined kinetic and modeling study of the catalytic center subsites of human angiogenin.
  Proc Natl Acad Sci U S A, 93, 804-808.  
8622921 N.Russo, V.Nobile, A.Di Donato, J.F.Riordan, and B.L.Vallee (1996).
The C-terminal region of human angiogenin has a dual role in enzymatic activity.
  Proc Natl Acad Sci U S A, 93, 3243-3247.  
8688423 O.Lequin, C.Albaret, F.Bontems, G.Spik, and J.Y.Lallemand (1996).
Solution structure of bovine angiogenin by 1H nuclear magnetic resonance spectroscopy.
  Biochemistry, 35, 8870-8880.
PDB code: 1gio
8633065 V.Nobile, B.L.Vallee, and R.Shapiro (1996).
Characterization of mouse angiogenin-related protein: implications for functional studies on angiogenin.
  Proc Natl Acad Sci U S A, 93, 4331-4335.  
7831307 K.A.Olson, J.W.Fett, T.C.French, M.E.Key, and B.L.Vallee (1995).
Angiogenin antagonists prevent tumor growth in vivo.
  Proc Natl Acad Sci U S A, 92, 442-446.  
7708754 K.R.Acharya, R.Shapiro, J.F.Riordan, and B.L.Vallee (1995).
Crystal structure of bovine angiogenin at 1.5-A resolution.
  Proc Natl Acad Sci U S A, 92, 2949-2953.
PDB code: 1agi
8159680 N.Russo, R.Shapiro, K.R.Acharya, J.F.Riordan, and B.L.Vallee (1994).
Role of glutamine-117 in the ribonucleolytic activity of human angiogenin.
  Proc Natl Acad Sci U S A, 91, 2920-2924.  
7702747 S.C.Allen, K.R.Acharya, K.A.Palmer, R.Shapiro, B.L.Vallee, and H.A.Scheraga (1994).
A comparison of the predicted and X-ray structures of angiogenin. Implications for further studies of model building of homologous proteins.
  J Protein Chem, 13, 649-658.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.