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Immunoreceptor
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PDB id
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1ah1
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* Residue conservation analysis
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PDB id:
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Immunoreceptor
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Title:
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Ctla-4, nmr, 20 structures
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Structure:
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Ctla-4. Chain: a. Fragment: extracellular n-terminal immunoglobulin v-like. Synonym: cd152. Engineered: yes. Other_details: tetrasaccharide core (glcnac, glcnac, fucose attached at n 78 and n 111
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: cho
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NMR struc:
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20 models
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Authors:
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W.J.Metzler,J.Bajorath,W.Fenderson,S.-Y.Shaw,R.Peach,K.L.Con J.Naemura,G.Leytze,T.B.Lavoie,L.Mueller,P.S.Linsley
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Key ref:
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W.J.Metzler
et al.
(1997).
Solution structure of human CTLA-4 and delineation of a CD80/CD86 binding site conserved in CD28.
Nat Struct Biol,
4,
527-531.
PubMed id:
DOI:
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Date:
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11-Apr-97
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Release date:
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15-Apr-98
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PROCHECK
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Headers
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References
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P16410
(CTLA4_HUMAN) -
Cytotoxic T-lymphocyte protein 4
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Seq:
Struc:
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223 a.a.
129 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 4 residue positions (black
crosses)
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Gene Ontology (GO) functional annotation
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Cellular component
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membrane
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1 term
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Biological process
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immune response
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1 term
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DOI no:
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Nat Struct Biol
4:527-531
(1997)
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PubMed id:
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Solution structure of human CTLA-4 and delineation of a CD80/CD86 binding site conserved in CD28.
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W.J.Metzler,
J.Bajorath,
W.Fenderson,
S.Y.Shaw,
K.L.Constantine,
J.Naemura,
G.Leytze,
R.J.Peach,
T.B.Lavoie,
L.Mueller,
P.S.Linsley.
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ABSTRACT
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The structure of human CTLA-4 reveals that residues Met 99, Tyr 100 and Tyr 104
of the M99YPPPY104 motif are adjacent to a patch of charged surface residues on
the A'GFCC' face of the protein. Mutation of these residues, which are conserved
in the CTLA-4/CD28 family, significantly reduces binding to CD80 and/or CD86,
implicating this patch as a ligand binding site.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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S.Zhu,
S.Liu,
L.Wan,
G.Yang,
H.Yang,
J.Cheng,
and
X.Lu
(2011).
Molecular cloning, expression and characterization of the functional domain of CTLA4 from the rhesus monkey, Macaca mulatta.
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Dev Comp Immunol, 35,
736-744.
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|
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Z.Chen,
R.Liu,
J.Che,
H.Shi,
Y.Hou,
Y.Liu,
L.Sun,
L.Tan,
C.Shan,
P.Hu,
and
Y.Cheng
(2011).
Qualitative and quantitative studies on human B7.1-Fc fusion protein and the application in pharmacokinetic study in rhesus monkeys.
|
| |
J Pharm Biomed Anal, 54,
133-140.
|
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L.Skrisovska,
M.Schubert,
and
F.H.Allain
(2010).
Recent advances in segmental isotope labeling of proteins: NMR applications to large proteins and glycoproteins.
|
| |
J Biomol NMR, 46,
51-65.
|
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T.Pentcheva-Hoang,
E.Corse,
and
J.P.Allison
(2009).
Negative regulators of T-cell activation: potential targets for therapeutic intervention in cancer, autoimmune disease, and persistent infections.
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| |
Immunol Rev, 229,
67-87.
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G.P.Bondinas,
A.K.Moustakas,
and
G.K.Papadopoulos
(2007).
The spectrum of HLA-DQ and HLA-DR alleles, 2006: a listing correlating sequence and structure with function.
|
| |
Immunogenetics, 59,
539-553.
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G.Szakonyi,
M.G.Klein,
J.P.Hannan,
K.A.Young,
R.Z.Ma,
R.Asokan,
V.M.Holers,
and
X.S.Chen
(2006).
Structure of the Epstein-Barr virus major envelope glycoprotein.
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| |
Nat Struct Mol Biol, 13,
996.
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PDB code:
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W.A.Teft,
M.G.Kirchhof,
and
J.Madrenas
(2006).
A molecular perspective of CTLA-4 function.
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Annu Rev Immunol, 24,
65-97.
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D.M.Compaan,
L.C.Gonzalez,
I.Tom,
K.M.Loyet,
D.Eaton,
and
S.G.Hymowitz
(2005).
Attenuating lymphocyte activity: the crystal structure of the BTLA-HVEM complex.
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| |
J Biol Chem, 280,
39553-39561.
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PDB code:
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E.J.Evans,
R.M.Esnouf,
R.Manso-Sancho,
R.J.Gilbert,
J.R.James,
C.Yu,
J.A.Fennelly,
C.Vowles,
T.Hanke,
B.Walse,
T.Hünig,
P.Sørensen,
D.I.Stuart,
and
S.J.Davis
(2005).
Crystal structure of a soluble CD28-Fab complex.
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Nat Immunol, 6,
271-279.
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PDB code:
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M.Srinivasan,
D.Lu,
R.Eri,
D.D.Brand,
A.Haque,
and
J.S.Blum
(2005).
CD80 binding polyproline helical peptide inhibits T cell activation.
|
| |
J Biol Chem, 280,
10149-10155.
|
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|
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J.F.Giguère,
S.Bounou,
J.S.Paquette,
J.Madrenas,
and
M.J.Tremblay
(2004).
Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle.
|
| |
J Virol, 78,
6222-6232.
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|
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X.Zhang,
J.C.Schwartz,
X.Guo,
S.Bhatia,
E.Cao,
M.Lorenz,
M.Cammer,
L.Chen,
Z.Y.Zhang,
M.A.Edidin,
S.G.Nathenson,
and
S.C.Almo
(2004).
Structural and functional analysis of the costimulatory receptor programmed death-1.
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| |
Immunity, 20,
337-347.
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PDB code:
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D.H.Margulies
(2003).
CD28, costimulator or agonist receptor?
|
| |
J Exp Med, 197,
949-953.
|
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|
|
|
|
|
F.Lühder,
Y.Huang,
K.M.Dennehy,
C.Guntermann,
I.Müller,
E.Winkler,
T.Kerkau,
S.Ikemizu,
S.J.Davis,
T.Hanke,
and
T.Hünig
(2003).
Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists.
|
| |
J Exp Med, 197,
955-966.
|
|
|
|
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|
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P.A.van der Merwe,
and
S.J.Davis
(2003).
Molecular interactions mediating T cell antigen recognition.
|
| |
Annu Rev Immunol, 21,
659-684.
|
|
|
|
|
|
|
S.Wang,
J.Bajorath,
D.B.Flies,
H.Dong,
T.Honjo,
and
L.Chen
(2003).
Molecular modeling and functional mapping of B7-H1 and B7-DC uncouple costimulatory function from PD-1 interaction.
|
| |
J Exp Med, 197,
1083-1091.
|
|
|
|
|
|
|
S.Anjos,
A.Nguyen,
H.Ounissi-Benkalha,
M.C.Tessier,
and
C.Polychronakos
(2002).
A common autoimmunity predisposing signal peptide variant of the cytotoxic T-lymphocyte antigen 4 results in inefficient glycosylation of the susceptibility allele.
|
| |
J Biol Chem, 277,
46478-46486.
|
|
|
|
|
|
|
S.Lazetic,
S.R.Leong,
J.C.Chang,
R.Ong,
G.Dawes,
and
J.Punnonen
(2002).
Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells.
|
| |
J Biol Chem, 277,
38660-38668.
|
|
|
|
|
|
|
S.Wang,
G.Zhu,
K.Tamada,
L.Chen,
and
J.Bajorath
(2002).
Ligand binding sites of inducible costimulator and high avidity mutants with improved function.
|
| |
J Exp Med, 195,
1033-1041.
|
|
|
|
|
|
|
C.A.Chambers
(2001).
The expanding world of co-stimulation: the two-signal model revisited.
|
| |
Trends Immunol, 22,
217-223.
|
|
|
|
|
|
|
D.A.Ostrov,
W.Shi,
J.C.Schwartz,
S.C.Almo,
and
S.G.Nathenson
(2000).
Structure of murine CTLA-4 and its role in modulating T cell responsiveness.
|
| |
Science, 290,
816-819.
|
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PDB code:
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|
|
D.L.Mueller
(2000).
T cells: A proliferation of costimulatory molecules.
|
| |
Curr Biol, 10,
R227-R230.
|
|
|
|
|
|
|
K.L.Bennett,
M.Kussmann,
P.Björk,
M.Godzwon,
M.Mikkelsen,
P.Sørensen,
and
P.Roepstorff
(2000).
Chemical cross-linking with thiol-cleavable reagents combined with differential mass spectrometric peptide mapping--a novel approach to assess intermolecular protein contacts.
|
| |
Protein Sci, 9,
1503-1518.
|
|
|
|
|
|
|
M.A.Bowen,
A.A.Aruffo,
and
J.Bajorath
(2000).
Cell surface receptors and their ligands: in vitro analysis of CD6-CD166 interactions.
|
| |
Proteins, 40,
420-428.
|
|
|
|
|
|
|
M.P.Pistillo,
P.L.Tazzari,
J.H.Ellis,
and
G.B.Ferrara
(2000).
Molecular characterization and applications of recombinant scFv antibodies to CD152 co-stimulatory molecule.
|
| |
Tissue Antigens, 55,
229-238.
|
|
|
|
|
|
|
S.Ikemizu,
R.J.Gilbert,
J.A.Fennelly,
A.V.Collins,
K.Harlos,
E.Y.Jones,
D.I.Stuart,
and
S.J.Davis
(2000).
Structure and dimerization of a soluble form of B7-1.
|
| |
Immunity, 12,
51-60.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
C.A.Chambers,
and
J.P.Allison
(1999).
Costimulatory regulation of T cell function.
|
| |
Curr Opin Cell Biol, 11,
203-210.
|
|
|
|
|
|
|
P.J.Erbel,
Y.Karimi-Nejad,
T.De Beer,
R.Boelens,
J.P.Kamerling,
and
J.F.Vliegenthart
(1999).
Solution structure of the alpha-subunit of human chorionic gonadotropin.
|
| |
Eur J Biochem, 260,
490-498.
|
|
|
PDB code:
|
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|
|
S.D.Nuttall,
M.J.Rousch,
R.A.Irving,
S.E.Hufton,
H.R.Hoogenboom,
and
P.J.Hudson
(1999).
Design and expression of soluble CTLA-4 variable domain as a scaffold for the display of functional polypeptides.
|
| |
Proteins, 36,
217-227.
|
|
|
|
|
|
|
P.Scheipers,
and
H.Reiser
(1998).
Role of the CTLA-4 receptor in T cell activation and immunity. Physiologic function of the CTLA-4 receptor.
|
| |
Immunol Res, 18,
103-115.
|
|
|
|
|
|
|
S.J.Davis,
S.Ikemizu,
M.K.Wild,
and
P.A.van der Merwe
(1998).
CD2 and the nature of protein interactions mediating cell-cell recognition.
|
| |
Immunol Rev, 163,
217-236.
|
|
|
|
|
|
The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
