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Endonuclease PDB id
1agi
Jmol
Contents
Protein chain
125 a.a. *
Waters ×91
* Residue conservation analysis
PDB id:
1agi
Name: Endonuclease
Title: Crystal structure of bovine angiogenin at 1.5 angstroms resolution
Structure: Angiogenin. Chain: a. Ec: 3.1.27.-
Source: Bos taurus. Cattle. Organism_taxid: 9913. Tissue: milk
Resolution:
1.50Å     R-factor:   0.203    
Authors: K.R.Acharya,R.Shapiro,J.F.Riordan,B.L.Vallee
Key ref: K.R.Acharya et al. (1995). Crystal structure of bovine angiogenin at 1.5-A resolution. Proc Natl Acad Sci U S A, 92, 2949-2953. PubMed id: 7708754 DOI: 10.1073/pnas.92.7.2949
Date:
06-Jan-95     Release date:   03-Apr-96    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P10152  (ANG1_BOVIN) -  Angiogenin-1
Seq:
Struc: 		148 a.a.
125 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   8 terms 
  Biological process     multicellular organismal development   14 terms 
  Biochemical function     nucleic acid binding     11 terms  

 

 
DOI no: 10.1073/pnas.92.7.2949 Proc Natl Acad Sci U S A 92:2949-2953 (1995)
PubMed id: 7708754  
 
 
Crystal structure of bovine angiogenin at 1.5-A resolution.
K.R.Acharya, R.Shapiro, J.F.Riordan, B.L.Vallee.
 
  ABSTRACT  
 
The capacity of angiogenin (Ang) to induce blood vessel growth is critically dependent on its ribonucleolytic activity. Crystallography and mutagenesis of human Ang have previously shown that its pyrimidine binding site is obstructed by Gln-117, implying that a conformational change is a key part of the mechanism of Ang action. The 1.5-A-resolution crystal structure of bovine Ang, in which glutamic acid is substituted for Gln-117, now confirms that a blocked active site is characteristic of these proteins. Indeed, the inactive conformation of bovine Ang is stabilized by a more extensive set of interactions than is that of human Ang. The three-dimensional structure of the putative receptor binding site is also well conserved in the two proteins. The Arg-Gly-Asp segment of this site in bovine Ang, which is replaced by Arg-Glu-Asn in human Ang, does not have a conformation typical of an integrin recognition site.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21050179 E.Pizzo, A.Merlino, M.Turano, I.Russo Krauss, F.Coscia, A.Zanfardino, M.Varcamonti, A.Furia, C.Giancola, L.Mazzarella, F.Sica, and G.D'Alessio (2010).
A new RNase sheds light on the RNase/angiogenin subfamily from zebrafish.
  Biochem J, 433, 345-355.
PDB codes: 3ljd 3lje 3ln8
  20827423 S.Li, and G.F.Hu (2010).
Angiogenin-mediated rRNA transcription in cancer and neurodegeneration.
  Int J Biochem Mol Biol, 1, 26-35.  
19549190 D.M.Monti, W.Yu, E.Pizzo, K.Shima, M.G.Hu, C.Di Malta, R.Piccoli, G.D'Alessio, and G.F.Hu (2009).
Characterization of the angiogenic activity of zebrafish ribonucleases.
  FEBS J, 276, 4077-4090.  
16301790 D.E.Holloway, G.B.Chavali, M.C.Hares, V.Subramanian, and K.R.Acharya (2005).
Structure of murine angiogenin: features of the substrate- and cell-binding regions and prospects for inhibitor-binding studies.
  Acta Crystallogr D Biol Crystallogr, 61, 1568-1578.
PDB codes: 2bwk 2bwl
  12842050 G.B.Chavali, A.C.Papageorgiou, K.A.Olson, J.W.Fett, G.Hu, R.Shapiro, and K.R.Acharya (2003).
The crystal structure of human angiogenin in complex with an antitumor neutralizing antibody.
  Structure, 11, 875-885.
PDB code: 1h0d
10070263 M.S.Madhusudhan, and S.Vishveshwara (1999).
Comparison of the dynamics of bovine and human angiogenin: a molecular dynamics study.
  Biopolymers, 49, 131-144.  
9578571 R.Shapiro (1998).
Structural features that determine the enzymatic potency and specificity of human angiogenin: threonine-80 and residues 58-70 and 116-123.
  Biochemistry, 37, 6847-6856.  
9266695 D.J.Strydom, M.D.Bond, and B.L.Vallee (1997).
An angiogenic protein from bovine serum and milk--purification and primary structure of angiogenin-2.
  Eur J Biochem, 247, 535-544.  
9461294 O.Lequin, H.Thüring, M.Robin, and J.Y.Lallemand (1997).
Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values.
  Eur J Biochem, 250, 712-726.
PDB code: 1awz
8622921 N.Russo, V.Nobile, A.Di Donato, J.F.Riordan, and B.L.Vallee (1996).
The C-terminal region of human angiogenin has a dual role in enzymatic activity.
  Proc Natl Acad Sci U S A, 93, 3243-3247.  
8688423 O.Lequin, C.Albaret, F.Bontems, G.Spik, and J.Y.Lallemand (1996).
Solution structure of bovine angiogenin by 1H nuclear magnetic resonance spectroscopy.
  Biochemistry, 35, 8870-8880.
PDB code: 1gio
8633065 V.Nobile, B.L.Vallee, and R.Shapiro (1996).
Characterization of mouse angiogenin-related protein: implications for functional studies on angiogenin.
  Proc Natl Acad Sci U S A, 93, 4331-4335.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.