PDBsum entry 1a7h

Eye-lens protein

PDB id

1a7h

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Contents

			Protein chains

					86 a.a. *

			Waters ×50

* Residue conservation analysis

PDB id:

1a7h

Links

Name:

Eye-lens protein

Title:

Gamma s crystallin c-terminal domain

Structure:

Gammas crystallin. Chain: a, b. Fragment: c-terminal domain. Engineered: yes. Mutation: yes

Source:

Bos taurus. Cattle. Organism_taxid: 9913. Cell_line: bl21. Organ: eye. Organelle: eye-lens. Gene: crygs. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.56Å

R-factor:

0.208

R-free:

0.295

Authors:

A.K.Basak,C.Slingsby

Key ref:

A.K.Basak et al. (1998). The C-terminal domains of gammaS-crystallin pair about a distorted twofold axis. Protein Eng, 11, 337-344. PubMed id: 9681865

Date:

13-Mar-98

Release date:

27-May-98

PROCHECK

	Headers

	References

Protein chains

P06504 (CRBS_BOVIN) - Gamma-crystallin S from Bos taurus

Seq: Struc:					178 a.a. 86 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 2 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.?

[IntEnz] [ExPASy] [KEGG] [BRENDA]

	Protein Eng 11:337-344 (1998)
PubMed id: 9681865

The C-terminal domains of gammaS-crystallin pair about a distorted twofold axis.
A.K.Basak, R.C.Kroone, N.H.Lubsen, C.E.Naylor, R.Jaenicke, C.Slingsby.

ABSTRACT

The 2-domain gammaS-crystallin, a highly conserved early evolutionary off-shoot of the gamma-crystallin family, is located in the water-rich region of eye lenses. The expressed C-terminal domain, gammaS-C, has been crystallized and the 2.56 A X-ray structure determined. There are two domains in the asymmetric unit which pair about a distorted twofold axis. One of the domains has an altered conformation in a highly conserved region of the protein, the tyrosine corner. The distorted gammaS-C dimer of domains is compared with the highly symmetrical, equivalent recombinant dimer of C-terminal domains from gammaB-crystallin. Sequence changes close to the interface, that distinguish gammaS from the other gamma-crystallins, are examined in order to evaluate their role in symmetrical domain pairing.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20725717

G.Kappé, A.G.Purkiss, S.T.van Genesen, C.Slingsby, and N.H.Lubsen (2010).
Explosive expansion of betagamma-crystallin genes in the ancestral vertebrate.

J Mol Evol, 71, 219-230.

16260758

Z.Wu, F.Delaglio, K.Wyatt, G.Wistow, and A.Bax (2005).
Solution structure of (gamma)S-crystallin by molecular fragment replacement NMR.

Protein Sci, 14, 3101-3114.

PDB codes:

1zwm 1zwo

15754064

C.Baraguey, F.Skouri-Panet, F.Bontems, A.Tardieu, G.Chassaing, and O.Lequin (2004).
(1)H, (15)N and (13)C resonance assignment of human gammaS-crystallin, a 21 kDa eye-lens protein.

J Biomol NMR, 30, 385-386.

11026675

M.Wenk, R.Herbst, D.Hoeger, M.Kretschmar, N.H.Lubsen, and R.Jaenicke (2000).
Gamma S-crystallin of bovine and human eye lens: solution structure, stability and folding of the intact two-domain protein and its separate domains.

Biophys Chem, 86, 95.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.