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488 a.a.
_CA
Key reference
DOI no: 10.1016/j.febslet.2006.04.017 FEBS Lett 580:2646-2652 (2006) PubMed id: 16647060
Crystal structure of AmyA lacks acidic surface and provide insights into protein stability at poly-extreme condition. N.Sivakumar, N.Li, J.W.Tang, B.K.Patel, K.Swaminathan. ABSTRACT
Here we report the first crystal structure of a protein, AmyA, a secretory alpha-amylase isolated from Halothermothrix orenii, which is both halophilic and thermophilic. The crystal structure was determined at 1.6 A resolution. AmyA lacks the conserved acidic surface, which is considered essential for protein stability at high salinity. Sedimentation velocity and CD experiments on AmyA reveal the formation of unique reversible poly-dispersed oligomers that show unusually high thermal stability. These studies provide valuable insight into the structural elements that contribute to the stability of AmyA at both physical and chemical extremes and their functional implications.
Selected figure(s)
Figure 1.
The above figure is reprinted by permission from the Federation of European Biochemical Societies: FEBS Lett (2006, 580, 2646-2652) copyright 2006.
Literature references that cite this PDB file's key reference
PubMed id Reference
19698123 J.A.Winter, P.Christofi, S.Morroll, and K.A.Bunting (2009).
The crystal structure of Haloferax volcanii proliferating cell nuclear antigen reveals unique surface charge characteristics due to halophilic adaptation.BMC Struct Biol, 9, 55.
19145256 K.Mavromatis, N.Ivanova, I.Anderson, A.Lykidis, S.D.Hooper, H.Sun, V.Kunin, A.Lapidus, P.Hugenholtz, B.Patel, and N.C.Kyrpides (2009).
Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii.PLoS ONE, 4, e4192.
17310272 S.Srimathi, G.Jayaraman, G.Feller, B.Danielsson, and P.R.Narayanan (2007).
Intrinsic halotolerance of the psychrophilic alpha-amylase from Pseudoalteromonas haloplanktis.Extremophiles, 11, 505-515. The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.
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