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344 a.a.
Key reference
DOI no: 10.1107/S0907444999013943 Acta Crystallogr D Biol Crystallogr 56:3 (2000) PubMed id: 10666621
The three-dimensional structure of a Trichoderma reesei beta-mannanase from glycoside hydrolase family 5. E.Sabini, H.Schubert, G.Murshudov, K.S.Wilson, M.Siika-Aho, M.Penttilä. ABSTRACT
The crystal structure of the catalytic core domain of beta-mannanase from the fungus Trichoderma reesei has been determined at a resolution of 1.5 A. The structure was solved using the anomalous scattering from a single non-isomorphous platinum complex with two heavy-metal sites in space group P2(1). The map computed with the experimental phases was enhanced by the application of an automated model building and refinement procedure using the amplitudes and experimental phases as observations. This approach is expected to be of more general application. The structure of the native enzyme and complexes with Tris-HCl and mannobiose are also reported: the mannobiose binds in subsites +1 and +2. The structure is briefly compared with that of the homologous beta-mannanase from the bacterium Thermomonospora fusca.
Selected figure(s)
Figure 1.
Figure 1 (a) Anomalous and (b) isomorphous difference Patterson syntheses at 1.65 Å resolution for the Pty complex: the Harker vectors of the two platinum sites are indicated.Figure 5.
Figure 5 As Fig. 4-. Aromatic residues (here Trp164 is shown) had a `hole' at the centre of the ring in the REFMAC/ARP map (c).The above figures are reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (2000, 56, 3-0) copyright 2000. Figures were selected by an automated process.
Literature references that cite this PDB file's key reference
PubMed id Reference
19912637 B.C.Do, T.T.Dang, J.G.Berrin, D.Haltrich, K.A.To, J.C.Sigoillot, and M.Yamabhai (2009).
Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-beta-mannosidase from Aspergillus niger BK01.Microb Cell Fact, 8, 59.
18755688 Y.Zhang, J.Ju, H.Peng, F.Gao, C.Zhou, Y.Zeng, Y.Xue, Y.Li, B.Henrissat, G.F.Gao, and Y.Ma (2008).
Biochemical and Structural Characterization of the Intracellular Mannanase AaManA of Alicyclobacillus acidocaldarius Reveals a Novel Glycoside Hydrolase Family Belonging to Clan GH-A.J Biol Chem, 283, 31551-31558.
PDB code: 3civ
17287210 L.E.Tailford, V.A.Money, N.L.Smith, C.Dumon, G.J.Davies, and H.J.Gilbert (2007).
Mannose foraging by Bacteroides thetaiotaomicron: structure and specificity of the beta-mannosidase, BtMan2A.J Biol Chem, 282, 11291-11299.
PDB code: 2je8
16240096 E.Papaleo, P.Fantucci, M.Vai, and L.De Gioia (2006).
Three-dimensional structure of the catalytic domain of the yeast beta-(1,3)-glucan transferase Gas1: a molecular modeling investigation.J Mol Model, 12, 237-248.
15840830 R.Bourgault, A.J.Oakley, J.D.Bewley, and M.C.Wilce (2005).
Three-dimensional structure of (1,4)-beta-D-mannan mannanohydrolase from tomato fruit.Protein Sci, 14, 1233-1241.
PDB code: 1rh9
12364331 L.K.Skov, O.Mirza, D.Sprogøe, I.Dar, M.Remaud-Simeon, C.Albenne, P.Monsan, and M.Gajhede (2002).
Oligosaccharide and sucrose complexes of amylosucrase. Structural implications for the polymerase activity.J Biol Chem, 277, 47741-47747.
PDB codes: 1mvy 1mw0 1mw1 1mw2 1mw3
10759859 E.Sabini, K.S.Wilson, M.Siika-aho, C.Boisset, and H.Chanzy (2000).
Digestion of single crystals of mannan I by an endo-mannanase from Trichoderma reesei.Eur J Biochem, 267, 2340-2344. The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.
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