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Oxidoreductase PDB id
1hfu
Jmol
Contents
Protein chain
500 a.a. *
Ligands
MAN-MAN
NAG-NDG
Metals
_CU ×3
Waters ×461
* Residue conservation analysis
PDB id:
1hfu
Name: Oxidoreductase
Title: Type-2 cu-depleted laccase from coprinus cinereus at 1.68 a resolution
Structure: Laccase 1. Chain: a. Engineered: yes
Source: Coprinus cinereus. Inky cap fungus. Organism_taxid: 5346. Expressed in: aspergillus oryzae. Expression_system_taxid: 5062.
Resolution:
1.68Å     R-factor:   0.184     R-free:   0.207
Authors: V.Ducros,A.M.Brzozowski,G.J.Davies
Key ref:
V.Ducros et al. (2001). Structure of the laccase from Coprinus cinereus at 1.68 A resolution: evidence for different 'type 2 Cu-depleted' isoforms. Acta Crystallogr D Biol Crystallogr, 57, 333-336. PubMed id: 11173497 DOI: 10.1107/S0907444900013779
Date:
08-Dec-00     Release date:   06-Dec-01    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q9Y780  (Q9Y780_COPCI) -  Laccase 1
Seq:
Struc: 		 
Seq:
Struc: 		539 a.a.
500 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.1.10.3.2  - Laccase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: 4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O
4 × benzenediol
Bound ligand (Het Group name = MAN)
matches with 72.00% similarity 		+ O(2)
 = 4 × benzosemiquinone
 + 2 × H(2)O
      Cofactor: Copper
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     oxidation-reduction process   1 term 
  Biochemical function     oxidoreductase activity     5 terms  

 

 
    Added reference    
 
 
DOI no: 10.1107/S0907444900013779 Acta Crystallogr D Biol Crystallogr 57:333-336 (2001)
PubMed id: 11173497  
 
 
Structure of the laccase from Coprinus cinereus at 1.68 A resolution: evidence for different 'type 2 Cu-depleted' isoforms.
V.Ducros, A.M.Brzozowski, K.S.Wilson, P.Ostergaard, P.Schneider, A.Svendson, G.J.Davies.
 
  ABSTRACT  
 
Laccases (E.C. 1.10.3.2; benzenediol oxygen oxidoreductases) couple the four-electron reduction of dioxygen to water to four one-electron oxidations of a reducing substrate. The three-dimensional structure of the 'blue' multi-copper oxidase laccase from the fungus Coprinus cinereus at 1.68 A reveals the structural basis for isoforms of the type 2 Cu-depleted species.
 
  Selected figure(s)  
 
Figure 1.
Figure 1 Cartoon representation of the three-dimensional structure of the C. cinereus laccase. The figure is colour-ramped from the N-terminus (blue) to the C-terminus (red). The Cu atoms are shown as shaded spheres, with the T1 site in blue and the T3 pair in yellow. This figure was produced using MOLSCRIPT/BOBSCRIPT (Esnouf, 1997[Esnouf, R. M. (1997). J. Mol. Graph. 15, 133-138.]; Kraulis, 1991[Kraulis, P. J. (1991). J. Appl. Cryst. 24, 946-950.]) and rendered using Raster3D (Merritt & Bacon, 1997[Merritt, E. A. & Bacon, D. J. (1997). Methods Enzymol. 277, 505-524.]; Merritt & Murphy, 1994[Merritt, E. A. & Murphy, M. E. P. (1994). Acta Cryst. D50, 869-873.]). The figure is in divergent (wall-eyed) stereo.
 
  The above figure is reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (2001, 57, 333-336) copyright 2001.  
  Figure was selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
19701722 M.B.Rajasekaran, S.Nilapwar, S.C.Andrews, and K.A.Watson (2010).
EfeO-cupredoxins: major new members of the cupredoxin superfamily with roles in bacterial iron transport.
  Biometals, 23, 1.  
19034452 C.Pezzella, F.Autore, P.Giardina, A.Piscitelli, G.Sannia, and V.Faraco (2009).
The Pleurotus ostreatus laccase multi-gene family: isolation and heterologous expression of new family members.
  Curr Genet, 55, 45-57.  
19346471 J.Yoon, S.Fujii, and E.I.Solomon (2009).
Geometric and electronic structure differences between the type 3 copper sites of the multicopper oxidases and hemocyanin/tyrosinase.
  Proc Natl Acad Sci U S A, 106, 6585-6590.  
18021071 O.V.Morozova, G.P.Shumakovich, M.A.Gorbacheva, S.V.Shleev, and A.I.Yaropolov (2007).
"Blue" laccases.
  Biochemistry (Mosc), 72, 1136-1150.  
  18084099 T.Skálová, J.Dohnálek, L.H.Ostergaard, P.R.Ostergaard, P.Kolenko, J.Dusková, and J.Hasek (2007).
Crystallization and preliminary X-ray diffraction analysis of the small laccase from Streptomyces coelicolor.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 63, 1077-1079.  
17334840 Y.Z.Hong, H.M.Zhou, X.M.Tu, J.F.Li, and Y.Z.Xiao (2007).
Cloning of a laccase gene from a novel basidiomycete Trametes sp. 420 and its heterologous expression in Pichia pastoris.
  Curr Microbiol, 54, 260-265.  
16944230 A.V.Lyashenko, I.Bento, V.N.Zaitsev, N.E.Zhukhlistova, Y.N.Zhukova, A.G.Gabdoulkhakov, E.Y.Morgunova, W.Voelter, G.S.Kachalova, E.V.Stepanova, O.V.Koroleva, V.S.Lamzin, V.I.Tishkov, C.Betzel, P.F.Lindley, and A.M.Mikhailov (2006).
X-ray structural studies of the fungal laccase from Cerrena maxima.
  J Biol Inorg Chem, 11, 963-973.  
  17012782 A.V.Lyashenko, N.E.Zhukhlistova, A.G.Gabdoulkhakov, Y.N.Zhukova, W.Voelter, V.N.Zaitsev, I.Bento, E.V.Stepanova, G.S.Kachalova, O.V.Koroleva, E.A.Cherkashyn, V.I.Tishkov, V.S.Lamzin, K.Schirwitz, E.Y.Morgunova, C.Betzel, P.F.Lindley, and A.M.Mikhailov (2006).
Purification, crystallization and preliminary X-ray study of the fungal laccase from Cerrena maxima.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 62, 954-957.
PDB code: 2h5u
16680453 P.Durão, I.Bento, A.T.Fernandes, E.P.Melo, P.F.Lindley, and L.O.Martins (2006).
Perturbations of the T1 copper site in the CotA laccase from Bacillus subtilis: structural, biochemical, enzymatic and stability studies.
  J Biol Inorg Chem, 11, 514-526.  
16234932 I.Bento, L.O.Martins, G.Gato Lopes, M.Arménia Carrondo, and P.F.Lindley (2005).
Dioxygen reduction by multi-copper oxidases; a structural perspective.
  Dalton Trans, 0, 3507-3513.
PDB codes: 1w6l 1w6w 1w8e 2bhf
12118243 N.Hakulinen, L.L.Kiiskinen, K.Kruus, M.Saloheimo, A.Paananen, A.Koivula, and J.Rouvinen (2002).
Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site.
  Nat Struct Biol, 9, 601-605.
PDB code: 1gw0
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.