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PDBsum entry 1cs3

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protein ligands metals links
Transcription PDB id
1cs3
Jmol
Contents
Protein chain
116 a.a. *
Ligands
GOL
Metals
_MG
Waters ×61
* Residue conservation analysis
PDB id:
1cs3
Name: Transcription
Title: Structure of btb/poz transcription repression domain from pr leukemia zinc finger oncoprotein
Structure: Zinc finger protein plzf. Chain: a. Fragment: btb/poz. Synonym: promyelocytic leukemia zinc finger protein. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Dimer (from PQS)
Resolution:
2.00Å     R-factor:   0.228     R-free:   0.272
Authors: X.Li,F.J.Rauscher 3rd,R.Marmorstein
Key ref: X.Li et al. (1999). Structure-function studies of the BTB/POZ transcriptional repression domain from the promyelocytic leukemia zinc finger oncoprotein. Cancer Res, 59, 5275-5282. PubMed id: 10537309 Ref: Full text
Date:
16-Aug-99     Release date:   27-Aug-99    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q05516  (ZBT16_HUMAN) -  Zinc finger and BTB domain-containing protein 16
Seq:
Struc:
 
Seq:
Struc:
673 a.a.
116 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
Full text Cancer Res 59:5275-5282 (1999)
PubMed id: 10537309  
 
 
Structure-function studies of the BTB/POZ transcriptional repression domain from the promyelocytic leukemia zinc finger oncoprotein.
X.Li, H.Peng, D.C.Schultz, J.M.Lopez-Guisa, F.J.Rauscher, R.Marmorstein.
 
  ABSTRACT  
 
The evolutionarily conserved BTB/POZ domain from the promyelocytic leukemia zinc finger (PLZF) oncoprotein mediates transcriptional repression through the recruitment of corepressor proteins containing histone deacetylases in acute promyelocytic leukemia. We have determined the 2.0 A crystal structure of the BTB/POZ domain from PLZF (PLZF-BTB/POZ), and have carried out biochemical analysis of PLZF-BTB/POZ harboring site-directed mutations to probe structure-function relationships. The structure reveals a novel alpha/beta homodimeric fold in which dimer interactions occur along two surfaces of the protein subunits. The conservation of BTB/POZ domain residues at the core of the protomers and at the dimer interface implies an analogous fold and dimerization mode for BTB/POZ domains from otherwise functionally unrelated proteins. Unexpectedly, the BTB/POZ domain forms dimer-dimer interactions in the crystals, suggesting a mode for higher-order protein oligomerization for BTB/POZ-mediated transcriptional repression. Biochemical characterization of PLZF-BTB/POZ harboring mutations in conserved residues involved in protein dimerization reveals that the integrity of the dimer interface is exquisitely sensitive to mutation and that dimer formation is required for wild-type levels of transcriptional repression. Interestingly, similar mutational analysis of residues within a pronounced protein cleft along the dimer interface, which had been implicated previously for interaction with corepressors, has negligible effects on dimerization or transcriptional repression. Together, these studies form a structure-function framework for understanding BTB/POZ-mediated oligomerization and transcriptional repression properties.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21301057 P.H.Wu, S.H.Hung, T.Ren, I.e.M.Shih, and Y.Tseng (2011).
Cell cycle-dependent alteration in NAC1 nuclear body dynamics and morphology.
  Phys Biol, 8, 015005.  
21193930 S.A.Devaney, S.E.Mate, J.M.Devaney, and E.P.Hoffman (2011).
Characterization of the ZBTB42 gene in humans and mice.
  Hum Genet, 129, 433-441.  
19361449 I.S.Dementieva, V.Tereshko, Z.A.McCrossan, E.Solomaha, D.Araki, C.Xu, N.Grigorieff, and S.A.Goldstein (2009).
Pentameric assembly of potassium channel tetramerization domain-containing protein 5.
  J Mol Biol, 387, 175-191.
PDB codes: 3drx 3dry 3drz
19170764 N.Ito, M.Watanabe-Matsui, K.Igarashi, and K.Murayama (2009).
Crystal structure of the Bach1 BTB domain and its regulation of homodimerization.
  Genes Cells, 14, 167-178.  
19115315 X.Ding, C.Luo, J.Zhou, Y.Zhong, X.Hu, F.Zhou, K.Ren, L.Gan, A.He, J.Zhu, X.Gao, and J.Zhang (2009).
The interaction of KCTD1 with transcription factor AP-2alpha inhibits its transactivation.
  J Cell Biochem, 106, 285-295.  
18221766 B.A.Wilton, S.Campbell, N.Van Buuren, R.Garneau, M.Furukawa, Y.Xiong, and M.Barry (2008).
Ectromelia virus BTB/kelch proteins, EVM150 and EVM167, interact with cullin-3-based ubiquitin ligases.
  Virology, 374, 82-99.  
  19052359 M.A.Stead, G.O.Rosbrook, J.M.Hadden, C.H.Trinh, S.B.Carr, and S.C.Wright (2008).
Structure of the wild-type human BCL6 POZ domain.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 64, 1101-1104.
PDB code: 3e4u
18358072 X.F.Ding, C.Luo, K.Q.Ren, J.Zhang, J.L.Zhou, X.Hu, R.S.Liu, Y.Wang, X.Gao, and J.Zhang (2008).
Characterization and expression of a human KCTD1 gene containing the BTB domain, which mediates transcriptional repression and homomeric interactions.
  DNA Cell Biol, 27, 257-265.  
17254023 L.Korutla, R.Degnan, P.Wang, and S.A.Mackler (2007).
NAC1, a cocaine-regulated POZ/BTB protein interacts with CoREST.
  J Neurochem, 101, 611-618.  
17189472 P.J.Stogios, L.Chen, and G.G.Privé (2007).
Crystal structure of the BTB domain from the LRF/ZBTB7 transcriptional regulator.
  Protein Sci, 16, 336-342.
PDB code: 2nn2
16542149 F.Schoenen, and B.Wirth (2006).
The zinc finger protein ZNF297B interacts with BDP1, a subunit of TFIIIB.
  Biol Chem, 387, 277-284.  
17120193 R.Perez-Torrado, D.Yamada, and P.A.Defossez (2006).
Born to bind: the BTB protein-protein interaction domain.
  Bioessays, 28, 1194-1202.  
15526281 A.J.Edgar, S.L.Dover, M.N.Lodrick, I.J.McKay, F.J.Hughes, and W.Turner (2005).
Bone morphogenetic protein-2 induces expression of murine zinc finger transcription factor ZNF450.
  J Cell Biochem, 94, 202-215.  
15726626 J.Zhou, X.Hu, X.Xiong, X.Liu, Y.Liu, K.Ren, T.Jiang, X.Hu, and J.Zhang (2005).
Cloning of two rat PDIP1 related genes and their interactions with proliferating cell nuclear antigen.
  J Exp Zoolog A Comp Exp Biol, 303, 227-240.  
16207353 P.J.Stogios, G.S.Downs, J.J.Jauhal, S.K.Nandra, and G.G.Privé (2005).
Sequence and structural analysis of BTB domain proteins.
  Genome Biol, 6, R82.  
16169482 S.Luke-Glaser, L.Pintard, C.Lu, P.E.Mains, and M.Peter (2005).
The BTB protein MEL-26 promotes cytokinesis in C. elegans by a CUL-3-independent mechanism.
  Curr Biol, 15, 1605-1615.  
14690607 K.F.Ahmad, A.Melnick, S.Lax, D.Bouchard, J.Liu, C.L.Kiang, S.Mayer, S.Takahashi, J.D.Licht, and G.G.Privé (2003).
Mechanism of SMRT corepressor recruitment by the BCL6 BTB domain.
  Mol Cell, 12, 1551-1564.
PDB codes: 1r28 1r29 1r2b
14527422 R.Geyer, S.Wee, S.Anderson, J.Yates, and D.A.Wolf (2003).
BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases.
  Mol Cell, 12, 783-790.  
11865059 A.Melnick, G.Carlile, K.F.Ahmad, C.L.Kiang, C.Corcoran, V.Bardwell, G.G.Prive, and J.D.Licht (2002).
Critical residues within the BTB domain of PLZF and Bcl-6 modulate interaction with corepressors.
  Mol Cell Biol, 22, 1804-1818.  
  11818025 L.Xu, L.Yang, K.Hashimoto, M.Anderson, G.Kohlhagen, Y.Pommier, and P.D'Arpa (2002).
Characterization of BTBD1 and BTBD2, two similar BTB-domain-containing Kelch-like proteins that interact with Topoisomerase I.
  BMC Genomics, 3, 1.  
12215542 S.Li, C.Xu, and R.W.Carthew (2002).
Phyllopod acts as an adaptor protein to link the sina ubiquitin ligase to the substrate protein tramtrack.
  Mol Cell Biol, 22, 6854-6865.  
12384587 S.Pagans, M.Ortiz-Lombardía, M.L.Espinás, J.Bernués, and F.Azorín (2002).
The Drosophila transcription factor tramtrack (TTK) interacts with Trithorax-like (GAGA) and represses GAGA-mediated activation.
  Nucleic Acids Res, 30, 4406-4413.  
11340155 T.Collins, J.R.Stone, and A.J.Williams (2001).
All in the family: the BTB/POZ, KRAB, and SCAN domains.
  Mol Cell Biol, 21, 3609-3615.  
10938130 A.Melnick, K.F.Ahmad, S.Arai, A.Polinger, H.Ball, K.L.Borden, G.W.Carlile, G.G.Prive, and J.D.Licht (2000).
In-depth mutational analysis of the promyelocytic leukemia zinc finger BTB/POZ domain reveals motifs and residues required for biological and transcriptional functions.
  Mol Cell Biol, 20, 6550-6567.  
11024164 D.Read, M.J.Butte, A.F.Dernburg, M.Frasch, and T.B.Kornberg (2000).
Functional studies of the BTB domain in the Drosophila GAGA and Mod(mdg4) proteins.
  Nucleic Acids Res, 28, 3864-3870.  
  10898795 K.D.Huynh, W.Fischle, E.Verdin, and V.J.Bardwell (2000).
BCoR, a novel corepressor involved in BCL-6 repression.
  Genes Dev, 14, 1810-1823.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.