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PDBsum entry 1ad6

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protein links
Transcription regulation PDB id
1ad6
Jmol
Contents
Protein chain
185 a.a. *
Waters ×76
* Residue conservation analysis
PDB id:
1ad6
Name: Transcription regulation
Title: Domain a of human retinoblastoma tumor suppressor
Structure: Retinoblastoma tumor suppressor. Chain: a. Fragment: domain a. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Cell_line: bl21. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Resolution:
2.30Å     R-factor:   0.201     R-free:   0.240
Authors: H.Y.Kim,Y.Cho
Key ref: H.Y.Kim and Y.Cho (1997). Structural similarity between the pocket region of retinoblastoma tumour suppressor and the cyclin-box. Nat Struct Biol, 4, 390-395. PubMed id: 9145110
Date:
21-Feb-97     Release date:   26-Aug-98    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P06400  (RB_HUMAN) -  Retinoblastoma-associated protein
Seq:
Struc:
 
Seq:
Struc:
928 a.a.
185 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     nucleus   1 term 
  Biological process     regulation of cell cycle   2 terms 

 

 
Nat Struct Biol 4:390-395 (1997)
PubMed id: 9145110  
 
 
Structural similarity between the pocket region of retinoblastoma tumour suppressor and the cyclin-box.
H.Y.Kim, Y.Cho.
 
  ABSTRACT  
 
The pocket region of retinoblastoma tumour suppressor (Rb) is essential for tumour suppressing activity. The Rb pocket is primarily composed of two domains, A and B. We have determined the X-ray crystal structure of domain A (residues 378-562) at 2.3 A resolution. Domain A consists of nine alpha-helices. The overall arrangement of helices in domain A is remarkably similar to the cyclin-box folds found in the crystal structures of cyclin A and TFIIB. This structure, along with domain B which is predicted to be homologous to the cyclin-box, suggests that the Rb pocket is composed of two cyclin-box fold domains. We present the structural/functional features of the Rb pocket, and the potential binding region for cellular or viral proteins within domain A.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
16638975 J.W.Harbour (2006).
Eye cancer: unique insights into oncogenesis: the Cogan Lecture.
  Invest Ophthalmol Vis Sci, 47, 1736-1745.  
16479010 O.Binda, J.S.Roy, and P.E.Branton (2006).
RBP1 family proteins exhibit SUMOylation-dependent transcriptional repression and induce cell growth inhibition reminiscent of senescence.
  Mol Cell Biol, 26, 1917-1931.  
11921304 C.Lee, and Y.Cho (2002).
Interactions of SV40 large T antigen and other viral proteins with retinoblastoma tumour suppressor.
  Rev Med Virol, 12, 81-92.  
  12225593 P.P.Claudio, T.Tonini, and A.Giordano (2002).
The retinoblastoma family: twins or distant cousins?
  Genome Biol, 3, reviews3012.  
11226179 H.Y.Kim, B.Y.Ahn, and Y.Cho (2001).
Structural basis for the inactivation of retinoblastoma tumor suppressor by SV40 large T antigen.
  EMBO J, 20, 295-304.
PDB code: 1gh6
11732181 P.C.John, M.Mews, and R.Moore (2001).
Cyclin/Cdk complexes: their involvement in cell cycle progression and mitotic division.
  Protoplasma, 216, 119-142.  
10982824 S.Yao, A.Neiman, and G.Prelich (2000).
BUR1 and BUR2 encode a divergent cyclin-dependent kinase-cyclin complex important for transcription in vivo.
  Mol Cell Biol, 20, 7080-7087.  
  10516048 L.A.LaPierre, D.L.Holzschu, P.R.Bowser, and J.W.Casey (1999).
Sequence and transcriptional analyses of the fish retroviruses walleye epidermal hyperplasia virus types 1 and 2: evidence for a gene duplication.
  J Virol, 73, 9393-9403.  
9609687 F.Hayashi, R.Ishima, D.Liu, K.I.Tong, S.Kim, D.Reinberg, S.Bagby, and M.Ikura (1998).
Human general transcription factor TFIIB: conformational variability and interaction with VP16 activation domain.
  Biochemistry, 37, 7941-7951.
PDB code: 1tfb
9634691 J.A.Endicott, and M.E.Noble (1998).
Structural principles in cell-cycle control: beyond the CDKs.
  Structure, 6, 535-541.  
  9841670 M.D.Mendenhall, and A.E.Hodge (1998).
Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae.
  Microbiol Mol Biol Rev, 62, 1191-1243.  
9914179 P.D.Adams, and W.G.Kaelin (1998).
Negative control elements of the cell cycle in human tumors.
  Curr Opin Cell Biol, 10, 791-797.  
9433129 M.E.Noble, J.A.Endicott, N.R.Brown, and L.N.Johnson (1997).
The cyclin box fold: protein recognition in cell-cycle and transcription control.
  Trends Biochem Sci, 22, 482-487.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.