8gs2

Electron Microscopy
2.84Å resolution

Structure of the Cas7-11-Csx29-guide RNA-target RNA (non-matching PFS) complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-105005 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (4 distinct):
CRISPR-associated RAMP family protein Chain: A
Molecule details ›
Chain: A
Length: 1616 amino acids
Theoretical weight: 185.6 KDa
Source organism: Desulfonema ishimotonii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A401FT36 (Residues: 1-1601; Coverage: 100%)
Gene name: DENIS_1082
Sequence domains: RAMP superfamily
CHAT domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 751 amino acids
Theoretical weight: 88.31 KDa
Source organism: Desulfonema ishimotonii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A401FT52 (Residues: 1-751; Coverage: 100%)
Gene name: DENIS_1081
Sequence domains: CHAT domain
crRNA Chain: R
Molecule details ›
Chain: R
Length: 95 nucleotides
Theoretical weight: 30.53 KDa
target RNA with non-matching PFS Chain: D
Molecule details ›
Chain: D
Length: 33 nucleotides
Theoretical weight: 10.66 KDa

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.84Å
Relevant EMDB volumes: EMD-34218
Expression system: Escherichia coli