› 7nl1

X-ray diffraction
2.33Å resolution

Crystal structure of cystathionine gamma-lyase from Toxoplasma gondii

Released:
DOI: 10.2210/pdb7nl1/pdb
PDB entry 7nl1 coloured by chain, front view.
PDB entry 7nl1 coloured by chain, side view.
PDB entry 7nl1 coloured by chain, top view.
Source organism: Toxoplasma gondii ME49
Primary publication:
Structural basis of the inhibition of cystathionine γ-lyase from Toxoplasma gondii by propargylglycine and cysteine.
Fernández-Rodríguez C, Conter C, Oyenarte I, Favretto F, Quintana I, Martinez-Chantar ML, Astegno A, Martínez-Cruz LA
Protein Sci 32 e4619 (2023)
PMID: 36883335

Function and Biology Details

Reaction catalysed: 
(1a) L-cystathionine = L-cysteine + 2-aminobut-2-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102880 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathione gamma lyase, putative Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 417 amino acids
Theoretical weight: 45.98 KDa
Source organism: Toxoplasma gondii ME49
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A125YN40 (Residues: 1-417; Coverage: 100%)
Gene name: TGME49_312930
Sequence domains: Cys/Met metabolism PLP-dependent enzyme

Ligands and Environments


Cofactor: Ligand PLP 8 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: C2221
Unit cell:
a: 126.53Å b: 138.991Å c: 472.997Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.21
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structural basis of the inhibition of cystathionine γ-lyase from Toxoplasma gondii by propargylglycine and cysteine.
Fernández-Rodríguez et al. (2023)