› 7bbc

Hybrid

Joint X-ray/neutron room temperature structure of perdeuterated PLL lectin in complex with perdeuterated L-fucose

Released:
DOI: 10.2210/pdb7bbc/pdb
PDB entry 7bbc coloured by chain, front view.
PDB entry 7bbc coloured by chain, side view.
PDB entry 7bbc coloured by chain, top view.
Source organism: Photorhabdus laumondii
Primary publication:
Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions.
Gajdos L, Blakeley MP, Kumar A, Wimmerová M, Haertlein M, Forsyth VT, Imberty A, Devos JM
Structure 29 1003-1013.e4 (2021)
PMID: 33765407

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181803 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bulb-type lectin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 381 amino acids
Theoretical weight: 41.94 KDa
Source organism: Photorhabdus laumondii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7N8J0 (Residues: 8-375; Coverage: 98%)
Gene name: plu0732
Sequence domains: Repeat of unknown function (DUF346)

Ligands and Environments

2 bound ligands:
Ligand FUL 3 x FUL
Ligand FUC 3 x FUC
No modified residues

Experiments and Validation Details

Experimental Method: X-ray diffraction, Neutron Diffraction
X-ray source: Xenocs GeniX 3D Cu HF, ILL BEAMLINE LADI III
Spacegroup: I222
Unit cell:
a: 72.665Å b: 89.21Å c: 159.235Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.121 0.14
Expression system: Escherichia coli

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Citations

4 citation in other articles

AQcalc: A web server that identifies weak molecular interactions in protein structures.
Afshinpour et al. (2023)
3 more