› 7qv8

X-ray diffraction
2.15Å resolution

Leishmania infantum BRC1 repeat in complex with LiRAD51

Released:
DOI: 10.2210/pdb7qv8/pdb
PDB entry 7qv8 coloured by chain, front view.
PDB entry 7qv8 coloured by chain, side view.
PDB entry 7qv8 coloured by chain, top view.
Source organism: Leishmania infantum
Primary publication:
Divergent binding mode for a protozoan BRC repeat to RAD51.
Pantelejevs T, Hyvönen M
Biochem J 479 1031-1043 (2022)
PMID: 35502837

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107323 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA repair protein RAD51 homolog Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 24.84 KDa
Source organism: Leishmania infantum
Expression system: Escherichia coli
UniProt:
  • Canonical: A4I3C9 (Residues: 134-376; Coverage: 60%)
Gene names: LINJ_28_0580, RAD51
Sequence domains: Rad51
BRCA2 OB1 domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 35 amino acids
Theoretical weight: 3.66 KDa
Source organism: Leishmania infantum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4HYJ9 (Residues: 108-140; Coverage: 3%)
Gene name: LINJ_20_0070
Sequence domains: BRCA2 repeat

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P41212
Unit cell:
a: 61.004Å b: 61.004Å c: 119.224Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.279 0.278 0.306
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

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