› 6wih

X-ray diffraction
1.9Å resolution

N-terminal mutation of ISCU2 (L35H36) traps Nfs1 Cys loop in the active site of ISCU2 without metal present. Structure of human mitochondrial complex Nfs1-ISCU2(L35H36)-ISD11 with E.coli ACP1 at 1.9 A resolution (NIAU)2.

Released:
DOI: 10.2210/pdb6wih/pdb
PDB entry 6wih coloured by chain, front view.
PDB entry 6wih coloured by chain, side view.
PDB entry 6wih coloured by chain, top view.
Source organisms:
Entry authors: Boniecki MT, Cygler M

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-141278 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Cysteine desulfurase Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 45.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y697 (Residues: 56-457; Coverage: 88%)
Gene names: HUSSY-08, NFS1, NIFS
Sequence domains: Aminotransferase class-V
LYR motif-containing protein 4 Chain: B
Molecule details ›
Chain: B
Length: 91 amino acids
Theoretical weight: 10.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HD34 (Residues: 1-91; Coverage: 100%)
Gene names: C6orf149, CGI-203, ISD11, LYRM4
Sequence domains: Complex 1 protein (LYR family)
Acyl carrier protein Chain: C
Molecule details ›
Chain: C
Length: 77 amino acids
Theoretical weight: 8.51 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A6A8 (Residues: 2-78; Coverage: 99%)
Gene names: JW1080, acpP, b1094
Sequence domains: Phosphopantetheine attachment site
Iron-sulfur cluster assembly enzyme ISCU Chain: D
Molecule details ›
Chain: D
Length: 143 amino acids
Theoretical weight: 15.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H1K1 (Residues: 35-167; Coverage: 80%)
Gene names: ISCU, NIFUN
Sequence domains: NifU-like N terminal domain

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
9 bound ligands:
Ligand PEG 9 x PEG
Ligand GOL 5 x GOL
Ligand EDO 38 x EDO
Ligand PG4 2 x PG4
Ligand P15 1 x P15
Ligand PGE 3 x PGE
Ligand EDT 1 x EDT
Ligand 8Q1 1 x 8Q1
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P41212
Unit cell:
a: 86.35Å b: 86.35Å c: 245.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.186
Expression system: Escherichia coli

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